Transient receptor potential channels as predictive marker and potential indicator of chemoresistance in colon cancer

Transient receptor potential(TRP)channels are strongly associated with colon cancer development and progression.This study leveraged a multivariate Cox regression model on publicly available datasets to construct a TRP channels-associated gene signature,with further validation of signature in real world samples from our hospital treated patient samples.Kaplan-Meier(K-M)survival analysis and receiver operating characteristic(ROC)curves were employed to evaluate this gene signature’s predictive accuracy and robustness in both training and testing cohorts,respectively.Additionally,the study utilized the CIBERSORT algorithm and single-sample gene set enrichment analysis to explore the signature’s immune infiltration landscape and underlying functional implications.The support vector machine algorithm was applied to evaluate the signature’s potential in predicting chemotherapy outcomes.The findings unveiled a novel three TRP channels-related gene signature(MCOLN1,TRPM5,and TRPV4)in colon adenocarcinoma(COAD).The ROC and K-M survival curves in the training dataset(AUC=0.761;p=1.58e-05)and testing dataset(AUC=0.699;p=0.004)showed the signature’s robust predictive capability for the overall survival of COAD patients.Analysis of the immune infiltration landscape associated with the signature revealed higher immune infiltration,especially an increased presence of M2 macrophages,in high-risk group patients compared to their low-risk counterparts.High-risk score patients also exhibited potential responsiveness to immune checkpoint inhibitor therapy,evident through increased CD86 and PD-1 expression profiles.Moreover,the TRPM5 gene within the signature was highly expressed in the chemoresistance group(p=0.00095)and associated with poor prognosis(p=0.036)in COAD patients,highlighting its role as a hub gene of chemoresistance.Ultimately,this signature emerged as an independent prognosis factor for COAD patients(p=6.48e-06)and expression of model gene are validated by public data and real-world patients.Overall,this bioinformatics study provides valuable insights into the prognostic implications and potential chemotherapy resistance mechanisms associated with TRPs-related genes in colon cancer.

Prediction of damage potential in mainshock–aftershock sequences using machine learning algorithms

Assessing the potential damage caused by earthquakes is crucial for a community’s emergency response.In this study,four machine learning(ML)methods—random forest,extremely randomized trees,AdaBoost(AB),and gradient boosting(GB)—were employed to develop prediction models for the damage potential of the mainshock(DIMS)and mainshock–aftershock sequences(DIMA).Building structures were modeled using eight single-degree-of-freedom(SDOF)systems with different hysteretic rules.A set of 662 recorded mainshock–aftershock(MS-AS)ground motions was selected from the PEER database.Seven intensity measures(IMs)were chosen to represent the characteristics of the mainshock and aftershock.The results revealed that the selected ML methods can well predict the structural damage potential of the SDOF systems,except for the AB method.The GB model exhibited the best performance,making it the recommended choice for predicting DIMS and DIMA among the four ML models.Additionally,the impact of input variables in the prediction was investigated using the shapley additive explanations(SHAP)method.The high-correlation variables were sensitive to the structural period(T).At T=1.0 s,the mainshock peak ground velocity(PGVM)and aftershock peak ground displacement(PGDA)significantly influenced the prediction of DIMA.When T increased to 5.0 s,the primary high-correlation factor of the mainshock IMs changed from PGVM to the mainshock peak ground displacement(PGDM);however,the highcorrelation variable of the aftershock IMs remained PGDA.The high-correlation factors for DIMS showed trends similar to those of DIMA.Finally,a table summarizing the first and second high-correlation variables for predicting DIMS and DIMA were provided,offering a valuable reference for parameter selection in seismic damage prediction for mainshock–aftershock sequences.

Massive Dirac particles based on gapped graphene with Rosen-Morse potential in a uniform magnetic field

We explore the gapped graphene structure in the two-dimensional plane in the presence of the Rosen-Morse potential and an external uniform magnetic field.In order to describe the corresponding structure,we consider the propagation of electrons in graphene as relativistic fermion quasi-particles,and analyze it by the wave functions of two-component spinors with pseudo-spin symmetry using the Dirac equation.Next,to solve and analyze the Dirac equation,we obtain the eigenvalues and eigenvectors using the Legendre differential equation.After that,we obtain the bounded states of energy depending on the coefficients of Rosen-Morse and magnetic potentials in terms of quantum numbers of principal n and spin-orbit k.Then,the values of the energy spectrum for the ground state and the first excited state are calculated,and the wave functions and the corresponding probabilities are plotted in terms of coordinates r.In what follows,we explore the band structure of gapped graphene by the modified dispersion relation and write it in terms of the two-dimensional wave vectors K_(x) and K_(y).Finally,the energy bands are plotted in terms of the wave vectors K_(x) and K_(y) with and without the magnetic term.

Gene targets with therapeutic potential in hepatocellular carcinoma

Hepatocellular carcinoma(HCC)is the third leading cause of cancer-related deaths worldwide.Major treatments include liver transplantation,resection,and chemotherapy,but the 5-year recurrence rate remains high.Late diagnosis often prevents surgical intervention,contributing to poor patient survival rates.Carcinogenesis in HCC involves genetic alterations that drive the transformation of normal cells into malignant ones.Enhancer of zeste homolog 2(EZH2),a key regulator of cell cycle progression,is frequently upregulated in HCC and is associated with advanced stages and poor prognosis,making it a potential biomarker.Additionally,signal transducer and activator of transcription 3,which binds to EZH2,affects disease staging and outcomes.Targeting EZH2 presents a promising therapeutic strategy.On the other hand,abnormal lipid metabolism is a hallmark of HCC and impacts prognosis.Fatty acid binding protein 5 is highly expressed in HCC tissues and correlates with key oncogenes,suggesting its potential as a biomarker.Other genes such as guanine monophosphate synthase,cell division cycle associated 5,and epidermal growth factor receptor provide insights into the molecular mechanisms of HCC,offering potential as biomarkers and therapeutic targets.

Grasshopper Incidence and Severity of Damage as Influenced by Cyanogenic Potential in Leaf Tissue of Cassava (Manihot esculenta Crantz)

This study assessed the effect of cyanogenic potential (CNP) in leaf tissue on grasshopper incidence and severity of damage in cassava for the identification of parents with desired complementary traits for crossing. The experiment was conducted at the Foya Wulleh, Njala experimental site in Sierra Leone during 2020 and 2021 cropping seasons in a randomized complete block design with three replications. A total of 30 genotypes comprising 26 breeding lines, two improved and two local genotypes were assessed. Results showed a significant (p < 0.05) linear relationship between leaf CNP and grasshopper infestation (incidence and severity of damage) among cassava genotypes. Findings showed that the higher leaf CNP, the lower the grasshopper infestation in cassava genotypes. About two genotypes (Cooksoon and Cocoa) had low leaf CNP;three genotypes (TR0020, TR0037 and TR0013) CNP had moderately low leaf CNP;eight genotypes (SLICASS 6, TR0029, TR0032, TR0011, TR0012, TR0016-1/17, TR0002 and TR0010) had intermediate leaf CNP;seven (TR0009, TR0015-1/17, TR0036, TR0022-1/17, SLICASS 4, TR0007 and TR0026-1/17) had moderately high leaf CNP;eight (TR0008, TR0019-1/17, TR0006, TR0005, TR0021, TR0021-1/17, TR0022 and TR0024-1/17) had high leaf CNP;and two genotypes (TR0001 and TR0018-1/17) had very high leaf CNP. This suggests the indirect dependence of leaf cyanogenic potential on grasshopper infestation (incidence and severity of damage) in cassava that could be exploited for the genetic improvement of cassava for improved resistance to grasshopper infestation, nutrition and utilization of the crop.

A Comprehensive Review of Exosomes with Therapeutic Potential in Cancer and Coeliac Disease

The aim of this review was to evaluate the therapeutic potential of exosomes, extracellular vesicles secreted by cells. They have emerged as potential therapeutic transporters for several diseases. This review provides an overview of exosomes’ therapeutic potential in cancer therapy and autoimmune conditions such as Coeliac Disease. The therapeutic effect is that the phospholipid-binding protein ANXA1 improves its anti-inflammatory properties. The review also analyzes the intricate processes of exosome production and composition ability to transport biomolecules such as proteins, microRNAs, and lipids, which promote intercellular communication and alter recipient cell behavior. Exosomes, linked to neurological disorders, cardiovascular disease, and cancer, present the means of targeted drug administration due to their innate specificity. Through genetic engineering and chemical modifications, exosomes can be tailored for specific purposes, demonstrating their versatility in targeted therapy. With ongoing research uncovering their therapeutic potential, exosomes present a promising frontier in novel medical treatments across various health conditions.

The Study of Mutual Benefits and Potential in China-Mongolia Agricultural Trade

This article aims to explore the mutual benefits and potential of agricultural trade between China and Mongolia,analyzing the current situation of agricultural resources,trade history,and policy environment of the two countries.The study finds that China-Mongolia agricultural trade shows significant economic complementarity.Trade activities not only promote economic growth in both countries but also deepen social and cultural exchanges.However,technical standards,political risks,and logistics costs remain major challenges facing trade cooperation.By strengthening policy coordination,investing in agricultural technology and infrastructure,and expanding the variety and markets of trade,the potential for cooperation in China-Mongolia agricultural trade can be further enhanced.These measures will help improve the efficiency and benefits of bilateral trade,achieving a win-win development.

Dissecting the key genomic regions underlying high yield potential in common wheat variety‘Kenong 9204’

The foundation parents play key roles in the genetic improvement of both yield potential and end-use quality in wheat.Characterizing the genetic basis that underlies certain beneficial traits in the foundation parents will provide theoretical reference for molecular breeding by a design approach.‘Kenong 9204’(KN9204)is a candidate foundation parent characterized by ideotype,high yield potential,and particularly high nitrogen fertilizer utilization.To better understand the genetic basis of its high yield potential,high throughput whole-genome re-sequencing(10×)was performed on KN9204,its parental lines and its derivatives.A high-resolution genetic composition map of KN9204 was constructed,which showed the parental origin of the favorable genomic segments based on the identification of excellent yield-related quantitative trait loci(QTL)from a bi-parental mapping population.Xiaoyan 693(XY693),a wheat–Thinopyrum ponticum partial amphidiploid,contributed a great deal to the high yield potential of KN9204,and three major stable QTLs from XY693 were fine mapped.The transmissibility of key genomic segments from KN9204 to its derivatives were delineated,indicating that haplotype blocks containing beneficial gene combinations were conserved along with directional selection by breeders.Evidence for selection sweeps in the breeding programs was identified.This study provides a theoretical reference for the breeding of high-yield wheat varieties by a molecular design approach.

A Comparative study on generalized Manev potential and Newtonian potential in perturbed restricted four-body problem

The motion of a test particle within the context of the restricted four-body problem(R4BP)driven by a new kind of potential,called the generalized Manev potential,with perturbations in the Coriolis and centrifugal forces is considered in this study.The system possesses eight libration points which were distributed on its plane of motion in different manner from those of the usual Newtonian potential.Unlike the case of the perturbed R4BP under Newtonian potential,where two of these librations are stable,all of them are unstable in linear sense under Manev potential.We found that a gradual perturbation in the centrifugal force causes the trajectories of motion to drift inward but the Coriolis force was proven to have no effect on the location of the libration points of the system.Using first order Lyapunov characteristic exponents,the dynamical behavior of the system is found irregular.We experimented with a high velocity stellar system(82 G.Eridani)to establish the applicability of the model in astrophysics.

Production of Railwayscape in urban environment:Analysing railway heritage tourism potential in Siliguri City,India

For a long time,it has been argued that the theories and practices devoted to urban planning and management should conform to the fundamental role of planning policies in the production of urban space,but not merely the spatial distribution of the produced services.Towards this wider connotation,this study introduces the notion of Railwayscape,grounded on the theory of‘The Production of Space’,to examine the role of railway station districts as catalysts of urban development through the social production of urban space.The present research sets out to establish the notion of Railwayscape and apply it in a railway heritage,i.e.,the Darjeeling Himalayan Railway(DHR)and its point of inception,Siliguri City,India.Accordingly,a criteria-based evaluation of four railway station districts(New Jalpaiguri,Siliguri Town,Siliguri Junction,and Sukna)in Siliguri and its surroundings was performed.The information regarding the selected four railway station districts is obtained through field observation and key informant consultation,supplemented by published literature and remote sensing data.This evaluation is succeeded by the strengths,weaknesses,opportunities and threats(SWOT)analysis accentuating the potential strengths,weaknesses,opportunities and threats associated with the selected four railway station districts and their prospects to become the potential Railwayscape.The results of this research show that there is no railway station district in Siliguri that can fully meet the demands of the locals and tourists,therefore,relfecting a lack of awareness of the historical values of these districts.The results also indicate that there are significant differences in the relative potentials of railway station districts to become the Railwayscape in urban environment.The outcomes of this research,therefore,are expected to encourage policy-making insitutions and practitioners to realise the‘place value’of some railway station districts and their potentials to yield better economic,social and structural virtue for a wide range of actors.

A review of pharmacological activity and application potential in food of tea polyphenols

Tea polyphenols(TP)is a class of polyhydroxy compounds isolated from tea.Modern biological and medical studies have shown that TP has many pharmacological activities,such as anti-inflammatory,anti-virus,anti-oxidation,anti-tumor and anti-radiation.Furthermore,these substances can be used as a potential drug component to positively guide the occurrence and development of certain diseases.Furthermore,because of the activities of TP,such as anti-oxidation and anti-bacteria,it can be applied in food preservation,color preservation,deodorization,and treatment of food processing by-products.Based on the research progress of TP in recent years,this paper summarizes the pharmacological activities of TP and expounds on its application potential in the field of food.In order to provide a theoretical reference for the research,development and utilization of TP.

Changes in the Tropical Cyclone Genesis Potential Index over the Western North Pacific in the SRES A2 Scenario

The Tropical Cyclone Genesis Potential Index （GPI） was employed to investigate possible impacts of global warming on tropical cyclone genesis over the western North Pacific （WNP）. The outputs of 20th century climate simulation by eighteen GCMs were used to evaluate the models＇ ability to reproduce tropical cyclone genesis via the GPI. The GCMs were found in general to reasonably reproduce the observed spatial distribution of genesis. Some of the models also showed ability in capturing observed temporal variation. Based on the evaluation, the models （CGCM3.1-T47 and IPSL-CM4） found to perform best when reproducing both spatial and temporal features were chosen to project future GPI. Results show that both of these models project an upward trend of the GPI under the SRES A2 scenario, however the rate of increase differs between them.

Enthalpies Estimation of Formation of Monosubstituted Alkanes by Interaction Potential Index

The interaction potential index IPI（X） of 16 Br, C1, I, NO2, CN, CHO, COOH, CH3, CH： kinds of substituents X （X---OH, SH, NH2, ：CH2, C-CH, Ph, COCH3, COOCH3） were proposed, which are derived from the experimental enthalpies of formation △fHФ （g） values of monosubstituted straight-chain alkanes. Based on the IPI（X） and polarizability effect index, a simple and effective model was constructed to estimate the △fHФ （g） values of monosubstituted alkanes RX （including the branched derivatives）. The present model takes into account not only the contributions of the alkyl R and the substituent X, but also the contribution of the interaction between R and X. Its stability and prediction ability was confirmed by the results of leave-one-out method. Compared with previous reported studies, the obtained equation can be used to estimate enthalpies of formation for much more kinds of monosubstituted alkanes with less parameters. Thus, it is recommended for the calculation of the △fHФ（g） for the RX.

ANALYTIC STUDY ON POTENTIAL INSTABILITY AND SPIRAL STRUCTURE OF RAIN CLUSTERS

This paper presents a study on potential instability and spiral structure of unstable rain clusters.First,we develop a linearized non-axisymmetrical mathematic model for rain clusters in circular cylindrical coordinates and acquire its analytic solution.Second,we discuss the potential instability of non-axisymmetrical rain clusters.Finally,we conclude that spiral structures can exist in rain clusters.Our analysis indicates that potential instability occurs when humid stratification coefficient is less than zero.Unstable growth rate increases with the increase of the absolute value for humid stratification coefficient.The simpler the vertical structure of perturbation,the thicker the inversion layer;additionally,the smaller the radius of the rain clusters,the larger the unstable growth rate.Simulation results agree well with those from observation and forecast.The spiral structure simulated by our model is similar to a radar echo,suggesting that rain clusters with spiral structures can occur in the atmosphere.In addition,they are generally close to the model solution in this work.

In silico identification of potential inhibitors targeting Streptococcus mutans sortase A

Dental caries is one of the most common chronic diseases and is caused by acid fermentation of bacteria adhered to the teeth. Streptococcus mutans （S. mutans） utilizes sortase A （SrtA） to anchor surface proteins to the cell wall and forms a biofilm to facilitate its adhesion to the tooth surface. Some plant natural products, especially several flavonoids, are effective inhibitors of SrtA. However, given the limited number of inhibitors and the development of drug resistance, the discovery of new inhibitors is urgent. Here, the high-throughput virtual screening approach was performed to identify new potential inhibitors of S. mutans SrtA. Two libraries were used for screening, and nine compounds that had the lowest scores were chosen for further molecular dynamics simulation, binding free energy analysis and absorption, distribution, metabolism, excretion and toxicity （ADMET） properties analysis. The results revealed that several similar compounds composed of benzofuran, thiadiazole and pyrrole, which exhibited good affinities and appropriate pharmacokinetic In addition, the carbonyl of these compounds can have a strategy for microbial infection disease therapy. parameters, were potential inhibitors to impede the catalysis of SrtA. key role in the inhibition mechanism. These findings can provide a new

A potential integration method for Birkhoffian system

This paper is intended to apply the potential integration method to the differential equations of the Birkhoffian system. The method is that, for a given Birkhoffian system, its differential equations are first rewritten as 2n first-order differential equations. Secondly, the corresponding partial differential equations are obtained by potential integration method and the solution is expressed as a complete integral. Finally, the integral of the system is obtained.

Yield Increasing and Quality Improving Effects of Smash-ridging Method("4453" Effects) and Its Potential in Benefiting the Nation and the People

Yuan Longping proposed that smash-ridging technology could be extend-ed widely nationwide. ln the research, smash-ridging technology reconstruct cultiva-tion layers with loose soils in agricultural fields, creating ＂4453＂ effects, as fol ows： ＂Four increases＂ include to increase loosen soil quantity in cultivation layers, soil nutri-ents use, ＂water pool＂ in soils, and ＂oxygen pool＂ in soils. Four reductions are to reduce soil erosion, carbon emission, salt content and heavy metal in soils. Five resistances refer to improve crop resistance capacity in terms of drought, high tem-perature, lower temperature, disease and lodging. Three improvements indicate to enhance photosynthetic efficiency over 10%, yield in 10%-30% and quality over 5%. lt is researched that without additional chemical fertilizer, yield could increase by 10% by labor force, animal, tractor or smash-ridging machine. What’s more, by smash-ridging cultivation, the depth can be 20 times or higher compared with tractor cultivation, with looser soils. lt is estimated that if smash-ridging cultivation is applied once in agricultural lands in China, present soil layers can be twice as deep as present. Specifical y, the thickness of loose soil-layers could be extended from 10-18 cm at present to 25-35 cm, and natural rainfal would increase by 40 bil ion cm3. After vitalization of soil nutrients, chemical fertilizer would decrease by 7 bil ion kg, and the increased c rops would feed more than 300 mil ion population as per yield at 7 50 kg/hm2.

Diagnosis of the ENSO modulation of tropical cyclogenesis over the southern South China Sea using a genesis potential index

The modulation of tropical cyclogenesis over the southern South China Sea （SSCS） by the El Nin o- Southern Oscillation （ENSO） is examined in October–December （OND）, when tropical cyclone （TC） activities are most active in this region. The results reveal that there were more TCs formed over the SSCS during La Nin a years and less TCs during El Nin o years. How different environmental factors （including low-level vorticity, mid-level relative humidity, vertical wind shear, and potential intensity） contribute to this influence is investigated, using a genesis potential （GP） index developed by Emanuel and Nolan. Composite anomalies of the GP index are produced for El Nin o and La Nin a years separately, which could account for the changes of TC frequency over the SSCS in different ENSO phases. The degree of contribution by each factor is determined quantitatively by producing composites of modified indices in which only one of the contributing factors varies, with the others set to climatology. The results show that the mid-level relative humidity makes the largest contribution to the ENSO modulation of tropical cyclogenesis over the SSCS. Although warmer sea surface temperatures （SSTs） and larger amount of evaporation from the ocean surface were observed over the SSCS during El Nin o years, anomalous descending motions due to the anomalous Walker circulations inhibited the upward transports of water vapor and led to less moisture contents in the middle troposphere, which suppressed TC formations.

Tropical Cyclone Genesis Potential Index over the Western North Pacific Simulated by CMIP5 Models

Tropical cyclone （TC） genesis over the western North Pacific （WNP） is analyzed using 23 CMIP5 （Coupled Model Intercomparison Project Phase 5） models and reanalysis datasets. The models are evaluated according to TC genesis potential index （GPI）. The spatial and temporal variations of the GPI are first calculated using three atmospheric reanalysis datasets （ERA-Interim, NCEP/NCAR Reanalysis- 1, and NCEP/DOE Reanalysis-2）. Spatial distributions of July-October-mean TC frequency based on the GPI from ERA-interim are more consistent with observed ones derived from IBTrACS global TC data. So, the ERA-interim reanalysis dataset is used to examine the CMIP5 models in terms of reproducing GPI during the period 1982-2005. Although most models possess deficiencies in reproducing the spatial distribution of the GPI, their multi- model ensemble （MME） mean shows a reasonable climatological GPI pattern characterized by a high GPI zone along 20°N in the WNP. There was an upward trend of TC genesis frequency during 1982 to 1998, followed by a downward trend. Both MME results and reanalysis data can represent a robust increasing trend during 1982-1998, but the models cannot simulate the downward trend after 2000. Analysis based on future projection experiments shows that the GPI exhibits no significant change in the first half of the 21st century, and then starts to decrease at the end of the 21st century under the representative concentration pathway （RCP） 2.6 scenario. Under the RCP8.5 scenario, the GPI shows an increasing trend in the vicinity of 20°N, indicating more TCs could possibly be expected over the WNP under future global warming.

Differential Proteomics Reveals the Potential Injury Mechanism Induced by Heavy Ion Radiation in Mice Ovaries

In the present study, we used a proteomics approach based on a two-dimensional electrophoresis （2-DE） reference map to investigate protein expression in the ovarian tissues of pubertal Swiss-Webster mice subjected to carbon ion radiation （CIR）. Among the identified proteins, ubiquitin carboxy-terminal hydrolase L1 （UCH-L1） is associated with the cell cycle[1] and that it influences proliferation in ovarian tissues. We analyzed the expression of UCH-L1 and the proliferation marker proliferation cell nuclear antigen （PCNA） following CIR using immunoblotting and immunofluorescence. The proteomics and biochemical results provide insight into the underlying mechanisms of CIR toxicity in ovarian tissues.