Mitochondria–cGAS–STING axis is a potential therapeutic target for senescence-dependent inflammaging-associated neurodegeneration

The word “senescence” comes from the Latin senescens, meaning “to begin to age”, and is characterized by a long-lasting but reversible block in proliferation, resulting from stress-induced cell cycle arrest of previously replication-competent cells.

THE BALANCE BETWEEN SUPPLY AND DEMAND OF WATER RESOURCES AND THE WATER-SAVING POTENTIAL FOR AGRICULTURE IN THE HEXI CORRIDOR

The Hexi Corridor is an important base of agriculture development inNorthwest China. According to recent statistics, there are 65. 94 x 10~8m^3 of water resourcesavailable in the Hexi Corridor. At present, net consumption in development and utilization is 43. 33x 10~8m^3. Water supply and demand reach a balance on the recent level of production, but loss ofevaporation and evapotranspiration is as much as 25. 69 x 1010~8m^3. So net use efficiency of waterresources is 59% Based on analyzing balance between water and land considering ecologicalenvironment at present, there exists the serious water shortage in the Shiyang River system whereirrigation lands have overloaded. There is a comparative balance between supply and demand of waterresource in the Heihe River system; and the Sule River system has some surplus water to extendirrigation land. Use of agriculture water accounts for 83. 3% and ecological forest and grass for 6.9% . The Hexi Corridor still has a great potential for water saving in agriculture production.Water-saving efficiency of irrigation is about 10% by using such traditional technologies as furrowand border-dike irrigation and small check irrigation, and water-saving with plastic film cover andtechniques of advanced sprinkler and drip/micro irrigation etc. can save more than 60% of irrigatedwater. Incremental irrigation area for water-saving potential in the Hexi Corridor has beenestimated as 56% - 197% to original irrigation area. So the second water sources can be developedfrom water saving agriculture in the Hexi Corridor under Development of the Western Part of China inlarge scale. This potential can be realized step by step through developing the water-savingmeasures, improving the ecological condition of oasis agriculture, and optimizing allocation ofwater resources in three river systems.

The water-saving potential of using micro-sprinkling irrigation for winter wheat production on the North China Plain

The shortage of groundwater resources is a considerable challenge for winter wheat production on the North China Plain.Water-saving technologies and procedures are thus urgently required.To determine the water-saving potential of using micro-sprinkling irrigation(MSI)for winter wheat production,field experiments were conducted from 2012 to 2015.Compared to traditional flooding irrigation(TFI),micro-sprinkling thrice with 90 mm water(MSI1)and micro-sprinkling four times with 120 mm water(MSI2)increased the water use efficiency by 22.5 and 16.2%,respectively,while reducing evapotranspiration by 17.6 and 10.8%.Regardless of the rainfall pattern,MSI(i.e.,MSI1 or MSI2)either stabilized or significantly increased the grain yield,while reducing irrigation water volumes by 20–40%,compared to TFI.Applying the same volumes of irrigation water,MSI(i.e.,MSI3,micro-sprinkling five times with 150 mm water)increased the grain yield and water use efficiency of winter wheat by 4.6 and 11.7%,respectively,compared to TFI.Because MSI could supply irrigation water more frequently in smaller amounts each time,it reduced soil layer compaction,and may have also resulted in a soil water deficit that promoted the spread of roots into the deep soil layer,which is beneficial to photosynthetic production in the critical period.In conclusion,MSI1 or MSI2 either stabilized or significantly increased grain yield while reducing irrigation water volumes by 20–40%compared to TFI,and should provide water-saving technological support in winter wheat production for smallholders on the North China Plain.

Development Potentials and Benefit Analysis of Efficient Water-saving Irrigation in Lixin County

On the basis of analyzing water resources,crop planning structure,and irrigation mode in Lixin County,potentials and benefits of developing efficient water-saving irrigation in the county were explored to provide references for its future water-saving irrigation.

The Potential Contribution of Subsurface Drip Irrigation to Water-Saving Agriculture in the Western USA

Water shortages within the western USA are resulting in the adoption of water-saving agricultural practices within this region. Among the many possible methods for saving water in agriculture, the adoption of subsurface drip irrigation （SDI） provides a potential solution to the problem of low water use efficiency. Other advantages of SDI include reduced NO3 leaching compared to surface irrigation, higher yields, a dry soil surface for improved weed control, better crop health, and harvest flexibility for many specialty crops. The use of SDI also allows the virtual elimination of crop water stress, the ability to apply water and nutrients to the most active part of the root zone, protection of drip lines from damage due to cultivation and tillage, and the ability to irrigate with wastewater while preventing human contact. Yet, SDI is used only on a minority of cropland in the arid western USA. Reasons for the limited adoption of SDI include the high initial capital investment required, the need for intensive management, and the urbanization that is rapidly consuming farmland in parts of the western USA. The contributions of SDI to increasing yield, quality, and water use efficiency have been demonstrated. The two major barriers to SDI sustainability in arid regions are economics （i.e., paying for the SDI system）, including the high cost of installation; and salt accumulation, which requires periodic leaching, specialized tillage methods, or transplanting of seedlings rather than direct-seeding. We will review advances in irrigation management with SDI.

Water-saving Potential in aeolian sand soil under straight tube and surface drip irrigation in Taklimakan Desert in Northwest China

Evaporation loss from the saturated soil beneath drip irrigation emitters highly influences the irrigation efficiency of drip krigation （D1]. Subsurface drip irrigation （SDI） is one good approach to curb this inefficiency, but in a new irrigation method, straight tube irrigation （STI）, the irrigation tubes do not need to be buried and thus STI is recommended to increase the irrigation efficiency under normal surface-applied DI. STI consists of only connectors and water-transference tubes that can directly transfer irrigation water from the lateral emitters in the drip line to the root zone of plants. Five-month field experiments were carried out in aeolian sand soil in the forest-belts of the Taklimakan Desert, which have poor water storage capacity, to compare the potential water saving between STI and DI. The preliminary results showed that, compared with DI, STI （1） improved the soil water content in soil depths from 40 to 100 cm under the soil surface; （2） achieved the same irrigation effects in relatively shorter irrigation durations; （3） had very little water loss due to deep seepage; and （4） formed a layer of dry sand about 10 to 30 cm thick immediately below the soil surface, which lessened evaporation loss of soil water beneath the emitters on the soil surface. This demonstrates that STI can maximize the water-saving potential of DI through the reduction of wetted soil perimeters on the soil surface. This is valuable information for water-saving engineering applications and projects with STI in arid and semiarid regions.

Water-saving potential evaluation of water-receiving regions in Shandong province on the East Route of the South-to-North Water Transfer Project of China

Taking 13 water-receiving areas on the East Route of the South-to-North Water Diversion Project(ERSNWDP)in Shandong Province as the study area,and comparing it with Jiangsu Province on the ERSNWDP and the Middle Route of the South-to-North(MRSNWDP),the current water-saving potential of the water-receiving areas within the municipalities of Shandong was analyzed.Different water-saving scenarios were constructed and analyzed with key water-saving indexes in various industries.These indexes include the effective utilization coefficient of farmland irrigation water,total water consumption of industrial sectors with an added value of over 10000 RMB,average leakage rate of the urban public water supply pipe network and the penetration rate of water-saving appliances.Based on the scenarios,comprehensive water-saving potential of the 13 water-receiving area cities was calculated.The results show that the water-saving potential of the study area is at a relative high level.However,some cities still have a certain amount of water-saving potential for agriculture and industry to be elevated.Under the recommended water-saving scenario,the water-saving potential is 1.134 billion m3,accounting for 5.33%of the current total water consumption,of which 460 million m3 is in agriculture,600 million m3 in industry,is and 74.20 million m3 in urban domestic sector.Comprehensive water-saving measures for the study area were proposed from the aspects of agricultural,industrial and domestic water uses.Agricultural and industrial water saving are more significant.The major cities for agricultural water saving include Jining City,Heze City,Weifang City and Jinan City;the focus cities of industrial water saving mainly include Weihai City,Jining City and Qingdao City and etc.;the key water-saving areas for urban use mainly include Zaozhuang City,Jining City and Heze City.

Agricultural water-saving potential and feasibility of developing semi-dryland farming in Henan Province

Based on the collected data in the current status of developing and utilizing water resources and imple- menting water-saving agriculture in Henan Province, and taking into account the influence of engineering, agro- nomic and management measures, the water-saving potential in past years and the feasibility of implementing semi-dryland farming were analyzed in Henan Province. Finally, specific technical measures of developing semi- drvland farming in different areas of Henan Province were orooosed.

Quantitative effect of kerogen type on the hydrocarbon generation potential of Paleogene lacustrine source rocks,Liaohe Western Depression,China

Kerogen types exert a decisive effect on the onset and capacity of hydrocarbon generation of source rocks.Lacustrine source rocks in the Liaohe Western Depression are characterized by thick deposition,high total organic carbon(TOC)content,various kerogen types,and a wide range of thermal maturity.Consequently,their hydrocarbon generation potential and resource estimation can be misinterpreted.In this study,geochemical tests,numerical analysis,hydrocarbon generation kinetics,and basin modeling were integrated to investigate the differential effects of kerogen types on the hydrocarbon generation potential of lacustrine source rocks.Optimized hydrocarbon generation and expulsion(HGE)models of different kerogen types were established quantitatively upon abundant Rock-Eval/TOC/vitrinite reflectance(R_(o))datasets.Three sets of good-excellent source rocks deposited in the fourth(Es4),third(Es3),and first(Es1)members of Paleogene Shahejie Formation,are predominantly types I-II_(1),II_(1)-II_(2),and II-III,respectively.The activation energy of types I-II_(2)kerogen is concentrated(180-230 kcal/mol),whereas that of type III kerogen is widely distributed(150-280 kcal/mol).The original hydrocarbon generation potentials of types I,II_(1),II_(2),and III kerogens are 790,510,270,and 85 mg/g TOC,respectively.The Ro values of the hydrocarbon generation threshold for type I-III source rocks gradually increase from 0.42%to 0.74%,and Ro values of the hydrocarbon expulsion threshold increase from 0.49%to 0.87%.Types I and II_(1)source rocks are characterized by earlier hydrocarbon generation,more rapid hydrocarbon expulsion,and narrower hydrocarbon generation windows than types II_(2)and III source rocks.The kerogen types also affect the HGE history and resource potential.Three types(conventional,tight,and shale oil/gas)and three levels(realistic,expected,and prospective)of hydrocarbon resources of different members in the Liaohe Western Depression are evaluated.Findings suggest that the Es3 member has considerable conventional and unconventional hydrocarbon resources.This study can quantitatively characterize the hydrocarbon generation potential of source rocks with different kerogen types,and facilitate a quick and accurate assessment of hydrocarbon resources,providing strategies for future oil and gas exploration.

Identification of transient receptor potential channel genes and functional characterization of TRPA1 in Spodoptera frugiperda

Spodoptera frugiperda is a highly destructive pest that has become a global problem due to its robust reproductive and migratory capabilities.Transient receptor potential(TRP)channels,which constitute a vast ion channel family,play pivotal roles in sensing the external environment and maintaining internal homeostasis in insects.TRP channels have been widely investigated for their critical roles in regulating various insect behaviors in recent years.In this study,we identified 15 TRP gene loci encoding 26 transcripts in the genome of S.frugiperda and analyzed their expression profiles at different developmental stages.The results revealed that S.frugiperda possesses four TRPC genes,six TRPA genes,one TRPM gene,two TRPV genes,one TRPN gene,and one TRPML gene,while a canonical TRPP is absent.Moreover,the SfruTRPA1 was functionally characterized using the Xenopus oocyte expression system.The results showed that SfruTRPA1 is activated by temperature increases from 20 to 45℃,and there is no significant desensitization after repeated stimuli within the same temperature range.Additionally,SfruTRPA1 is activated by certain natural chemicals,including allyl isothiocyanate(AITC)and cinnamaldehyde(CA).These findings provide valuable insights to the TRP genes in S.frugiperda.

Transient receptor potential channels as predictive marker and potential indicator of chemoresistance in colon cancer

Transient receptor potential(TRP)channels are strongly associated with colon cancer development and progression.This study leveraged a multivariate Cox regression model on publicly available datasets to construct a TRP channels-associated gene signature,with further validation of signature in real world samples from our hospital treated patient samples.Kaplan-Meier(K-M)survival analysis and receiver operating characteristic(ROC)curves were employed to evaluate this gene signature’s predictive accuracy and robustness in both training and testing cohorts,respectively.Additionally,the study utilized the CIBERSORT algorithm and single-sample gene set enrichment analysis to explore the signature’s immune infiltration landscape and underlying functional implications.The support vector machine algorithm was applied to evaluate the signature’s potential in predicting chemotherapy outcomes.The findings unveiled a novel three TRP channels-related gene signature(MCOLN1,TRPM5,and TRPV4)in colon adenocarcinoma(COAD).The ROC and K-M survival curves in the training dataset(AUC=0.761;p=1.58e-05)and testing dataset(AUC=0.699;p=0.004)showed the signature’s robust predictive capability for the overall survival of COAD patients.Analysis of the immune infiltration landscape associated with the signature revealed higher immune infiltration,especially an increased presence of M2 macrophages,in high-risk group patients compared to their low-risk counterparts.High-risk score patients also exhibited potential responsiveness to immune checkpoint inhibitor therapy,evident through increased CD86 and PD-1 expression profiles.Moreover,the TRPM5 gene within the signature was highly expressed in the chemoresistance group(p=0.00095)and associated with poor prognosis(p=0.036)in COAD patients,highlighting its role as a hub gene of chemoresistance.Ultimately,this signature emerged as an independent prognosis factor for COAD patients(p=6.48e-06)and expression of model gene are validated by public data and real-world patients.Overall,this bioinformatics study provides valuable insights into the prognostic implications and potential chemotherapy resistance mechanisms associated with TRPs-related genes in colon cancer.

Geochemical fingerprints and hydrocarbon potential of Paleocene mudrocks in the Tano Basin, Ghana: insights from biomarkers and stable carbon isotopes

The Paleocene mudrocks in Ghana’s Tano Basin have received limited attention despite ongoing efforts to explore hydrocarbon resources.A thorough geochemical analysis is imperative to assess these mudrocks’petroleum generation potential and formulate effective exploration strategies.In this study,a comprehensive geochemical analysis was carried out on ten Paleocene rock cuttings extracted from TP-1,a discovery well within the Tano Basin.Various analytical techniques,including total organic carbon(TOC)analysis,Rock–Eval pyrolysis,gas chromatography-mass spectrometry,and isotope ratio-mass spectrometry,were employed to elucidate their hydrocar-bon potential and organic facies.Thefindings in this study were subsequently compared to existing geochemical data on Paleocene source rocks in the South Atlantic marginal basins.The results indicated that the Paleocene samples have TOC content ranging from 0.68 to 2.93 wt%.The prevalent kerogen types identified in these samples were Type Ⅱ and Type Ⅲ.Molecular and isotope data suggest that the organic matter found in the Paleocene mudrocks can be traced back to land plants and lower aquatic organisms.These mudrocks were deposited in a transi-tional environment withfluctuating water salinity,charac-terized by sub-oxic redox conditions.Maturity indices,both bulk and molecular,indicated a spectrum of maturity levels within the Paleocene mudrocks,spanning from immature to marginally mature,with increasing maturity observed with greater depth.In comparison,the organic composition and depositional environments of Paleocene source rocks in the Tano Basin closely resemble those found in the Niger Delta Basin,Douala,and Kribi-Campo Basins,the Kwanza Formation in Angola,and certain Brazilian marginal basins.However,it is worth noting that Paleocene source rocks in some of the basins,such as the Niger Delta and Brazilian marginal basins,exhibit rela-tively higher thermal maturity levels compared to those observed in the current Paleocene samples from the Tano Basin.In conclusion,the comprehensive geochemical analysis of Paleocene mudrocks within Ghana’s Tano Basin has unveiled their marginal hydrocarbon generation potential.The shared geochemical characteristics between the Paleocene mudrocks in the Tano Basin and those in the nearby South Atlantic marginal basins offer valuable insights into source rock quality,which is crucial for shaping future strategies in petroleum exploration in this region.

Natural gas exploration potential and favorable targets of Permian Fengcheng Formation in the western Central Depression of Junggar Basin,NW China

Based on the organic geochemical data and the molecular and stable carbon isotopic compositions of natural gas of the Lower Permian Fengcheng Formation in the western Central Depression of Junggar Basin,combined with sedimentary environment analysis and hydrocarbon-generating simulation,the gas-generating potential of the Fengcheng source rock is evaluated,the distribution of large-scale effective source kitchen is described,the genetic types of natural gas are clarified,and four types of favorable exploration targets are selected.The results show that:(1)The Fengcheng Formation is a set of oil-prone source rocks,and the retained liquid hydrocarbon is conducive to late cracking into gas,with characteristics of high gas-generating potential and late accumulation;(2)The maximum thickness of Fengcheng source rock reaches 900 m.The source rock has entered the main gas-generating stage in Penyijingxi and Shawan sags,and the area with gas-generating intensity greater than 20×10^(8) m^(3)/km^(2) is approximately 6500 km^(2).(3)Around the western Central Depression,highly mature oil-type gas with light carbon isotope composition was identified to be derived from the Fengcheng source rocks mainly,while the rest was coal-derived gas from the Carboniferous source rock;(4)Four types of favorable exploration targets with exploration potential were developed in the western Central Depression which are structural traps neighboring to the source,stratigraphic traps neighboring to the source,shale-gas type within the source,and structural traps within the source.Great attention should be paid to these targets.

Bimetallic In_(2)O_(3)/Bi_(2)O_(3) Catalysts Enable Highly Selective CO_(2) Electroreduction to Formate within Ultra-Broad Potential Windows

CO_(2)electrochemical reduction reaction(CO_(2)RR)to formate is a hopeful pathway for reducing CO_(2)and producing high-value chemicals,which needs highly selective catalysts with ultra-broad potential windows to meet the industrial demands.Herein,the nanorod-like bimetallic ln_(2)O_(3)/Bi_(2)O_(3)catalysts were successfully synthesized by pyrolysis of bimetallic InBi-MOF precursors.The abundant oxygen vacancies generated from the lattice mismatch of Bi_(2)O_(3)and ln_(2)O_(3)reduced the activation energy of CO_(2)to*CO_(2)·^(-)and improved the selectivity of*CO_(2)·^(-)to formate simultaneously.Meanwhile,the carbon skeleton derived from the pyrolysis of organic framework of InBi-MOF provided a conductive network to accelerate the electrons transmission.The catalyst exhibited an ultra-broad applied potential window of 1200 mV(from-0.4 to-1.6 V vs RHE),relativistic high Faradaic efficiency of formate(99.92%)and satisfactory stability after 30 h.The in situ FT-IR experiment and DFT calculation verified that the abundant oxygen vacancies on the surface of catalysts can easily absorb CO_(2)molecules,and oxygen vacancy path is dominant pathway.This work provides a convenient method to construct high-performance bimetallic catalysts for the industrial application of CO_(2)RR.

(2+1)维potential Kadomtsev-Petviashvili方程的有理解

基于Hirota双线性方法,求得一类potential Kadomtsev-Petviashvili(pKP)方程的有理解,并对该解作出了证明,进而求得方程的lump解、高阶lump解和多lump解,同时通过图像展现了两种lump波的不同碰撞方式,分析了多个lump波碰撞的动力学性质。

Sequestration of CO_(2)by concrete and natural minerals-current status,future potential,and additional benefits

Concrete structures are some of the largest constructions in human civilization.Their manufacture releases CO_(2)into atmosphere,which is partially readsorbed by standing structures,and further release occurs when they are demolished.Concrete is chemically similar to basaltic minerals,both adsorb CO_(2)where they are exposed on the earth's surface.Sequestration of CO_(2)is beneficial to reduce atmospheric concentrations,and thus limit future temperature increases.Therefore,multiple options are being examined for CO_(2)sequestration.For the first time,we compare the CO_(2)sequestration capacity of these two materials.We review previous work quantifying CO_(2)sequestration capacity of both materials and for the first time,compare their potential quantitative roles.Costs of that are compiled,to the extent they have been examined.Costly grinding of these materials to small particle sizes accelerates CO_(2)sequestration,and mycorrhizae in agricultural soils might reduce the associated costs.Both these materials can improve nutrient status in agricultural soils,and limit acidification from external nitrogen fertilization.Limitations are discussed in terms of land-use and material availability,and soil pH conditions.We call for further experiments with these materials that compare CO_(2)sequestration and other biogeochemical processes in agricultural systems across climates,carried out especially where such materials are conveniently available.

Probing the electric double layer structure at nitrogen-doped graphite electrodes by constant-potential molecular dynamics simulations

Electric double layer(EDL)is a critical topic in electrochemistry and largely determines the working performance of lithium batteries.However,atomic insights into the EDL structures on heteroatom-modified graphite anodes and EDL evolution with electrode potential are very lacking.Herein,a constant-potential molecular dynamics(CPMD)method is proposed to probe the EDL structure under working conditions,taking N-doped graphite electrodes and carbonate electrolytes as an example.An interface model was developed,incorporating the electrode potential and atom electronegativities.As a result,an insightful atomic scenario for the EDL structure under varied electrode potentials has been established,which unveils the important role of doping sites in regulating both the EDL structures and the following electrochemical reactions at the atomic level.Specifically,the negatively charged N atoms repel the anions and adsorb Li~+at high and low potentials,respectively.Such preferential adsorption suggests that Ndoped graphite can promote Li~+desolvation and regulate the location of Li~+deposition.This CPMD method not only unveils the mysterious function of N-doping from the viewpoint of EDL at the atomic level but also applies to probe the interfacial structure on other complicated electrodes.

Mass Increase with Strong and Gravitational Potentials, and Mass Defect with Electromagnetic Potential

The proposal is “mass increases due to strong and gravitational potentials, while it decreases due to Electromagnetic potential”. This proposal explains the big difference in mass between hadrons (protons, neutrons, & mesons) and their components (quarks), mass difference between nucleus and its individual components (protons and neutrons), massless of gamma photons, abnormal masses of mesons and bosons, and the excess in galaxy masses (dark matter). Also, this proposal shows the exact relation between mass and energy: Strong Potential=−3.04mc2| Electric Potential |=−5.57×10−3mc2Gravitational Potential=−1.22×10−7mc2where m represents the excess in mass due to strong potential, or gravitational potential and represents the decrease in mass due to electromagnetic potential. Released energy here equals potential energy and doesn’t equal decrease in mass using the formula E = mc2. Released energy is transferred to heat, photons, kinetic energy… Finally, proposal will try to describe the relation between photon energy and mass of its components using the general equation of kinetic energy: Photon Energy=1/2mc2m is the sum of the individual masses of its components, while the total mass of photon is zero.

Krüppel-like factor 2(KLF2),a potential target for neuroregeneration

Neurological disorders and injuries lead to the impairment or depletion of neurons in terms of quantity,structure,or function,resulting in the loss of cognitive,emotional,and physical abilities in human beings.This,in turn,accompanies the shortfall of valuable human resources and economic potential,causing a detriment to society.Addressing these challenges,therefore,becomes imperative not only from a humanitarian perspective but also as a means of preserving human capital and bolstering economic prosperity.

Grasshopper Incidence and Severity of Damage as Influenced by Cyanogenic Potential in Leaf Tissue of Cassava (Manihot esculenta Crantz)

This study assessed the effect of cyanogenic potential (CNP) in leaf tissue on grasshopper incidence and severity of damage in cassava for the identification of parents with desired complementary traits for crossing. The experiment was conducted at the Foya Wulleh, Njala experimental site in Sierra Leone during 2020 and 2021 cropping seasons in a randomized complete block design with three replications. A total of 30 genotypes comprising 26 breeding lines, two improved and two local genotypes were assessed. Results showed a significant (p < 0.05) linear relationship between leaf CNP and grasshopper infestation (incidence and severity of damage) among cassava genotypes. Findings showed that the higher leaf CNP, the lower the grasshopper infestation in cassava genotypes. About two genotypes (Cooksoon and Cocoa) had low leaf CNP;three genotypes (TR0020, TR0037 and TR0013) CNP had moderately low leaf CNP;eight genotypes (SLICASS 6, TR0029, TR0032, TR0011, TR0012, TR0016-1/17, TR0002 and TR0010) had intermediate leaf CNP;seven (TR0009, TR0015-1/17, TR0036, TR0022-1/17, SLICASS 4, TR0007 and TR0026-1/17) had moderately high leaf CNP;eight (TR0008, TR0019-1/17, TR0006, TR0005, TR0021, TR0021-1/17, TR0022 and TR0024-1/17) had high leaf CNP;and two genotypes (TR0001 and TR0018-1/17) had very high leaf CNP. This suggests the indirect dependence of leaf cyanogenic potential on grasshopper infestation (incidence and severity of damage) in cassava that could be exploited for the genetic improvement of cassava for improved resistance to grasshopper infestation, nutrition and utilization of the crop.