On Potentially Graphical Sequences of G-E(H)

A loopless graph on n vertices in which vertices are connected at least by a and at most by b edges is called a(a,b,n)-graph. A(b,b,n)-graph is called(b,n)-graph and is denoted by K_n^b(it is a complete graph), its complement by K_n^b. A non increasing sequence π =(d_1,…,d_n) of nonnegative integers is said to be(a,b,n) graphic if it is realizable by an(a,b,n)-graph. We say a simple graphic sequence π=(d_1,…,d_n) is potentially K_4-K_2∪K_2-graphic if it has a a realization containing an K_4-K_2∪K_2 as a subgraph where K_4 is a complete graph on four vertices and K_2∪K_2 is a set of independent edges. In this paper, we find the smallest degree sum such that every n-term graphical sequence contains K_4-K_2∪K_2 as subgraph.

Characterizing C6＋P2-graphic Sequences

For a given graph H, a graphic sequence π =(d1, d2, ···, dn) is said to be potentially H-graphic if π has a realization containing H as a subgraph. In this paper, we characterize the potentially C6+ P2-graphic sequences where C6+ P2 denotes the graph obtained from C6 by adding two adjacent edges to the three pairwise nonadjacent vertices of C6. Moreover, we use the characterization to determine the value of σ(C6+ P2, n).

转录组测序分析黄连总碱抗脑缺血的作用机制

背景:黄连清热燥湿、泻火解毒,黄连及其成分对脑缺血有显著的保护作用,基于网络药理学和转录组测序探究黄连总碱抗脑缺血的作用机制。目的:在明确黄连总碱对脑缺血大鼠保护作用的基础上,基于网络药理学和转录组测序技术探讨黄连总碱干预脑缺血的作用机制。方法:将SD大鼠随机分为假手术组、缺血再灌注组、阳性药物组和黄连总碱组,后3组采用改良线栓法制备大脑中动脉阻塞的缺血再灌注模型,假手术组不插入线栓,其余手术操作相同。通过TTC染色、Longa 5神经功能缺损评分、苏木精-伊红染色、尼氏染色评价黄连总碱对脑缺血再灌注模型大鼠的脑保护作用。对假手术组、缺血再灌注组、黄连总碱组大鼠脑组织进行转录组测序,通过差异表达基因筛选、基因本体论分析、京都基因与基因组百科全书分析以及转录组学与网络药理学关联分析,阐明黄连总碱干预脑缺血的作用特点,最后通过ELISA检测及免疫荧光染色对黄连总碱干预脑缺血的关键靶点进行验证。结果与结论:①黄连总碱可降低缺血再灌注模型大鼠Longa 5神经功能缺损评分及脑梗死面积,增加神经元、尼氏小体数目;②黄连总碱治疗后差异表达基因的基因本体论功能富集分析包括炎症反应、分裂原活化蛋白激酶级联的正调控等生物学过程;京都基因与基因组百科全书通路富集分析主要涉及白细胞介素17信号通路、神经活性配体-受体相互作用、环磷腺苷信号通路等通路;③转录组分析发现黄连总碱主要调控的基因有前列腺素内过氧化物合酶2、脑源性神经营养因子、瞬间受体电位A1等;④采用网络药理学分析发现,黄连总碱中9个成分可能通过87个成分靶点关联到过氧化物合酶2、脑源性神经营养因子及瞬间受体电位A1而发挥作用;⑤ELISA检测及免疫荧光染色结果进一步证实,黄连总碱调控脑缺血再灌注模型大鼠过氧化物合酶2、脑源性神经营养因子和瞬间受体电位A1的表达;⑥提示黄连总碱能明显改善脑缺血再灌注模型大鼠损伤,可能通过调控过氧化物合酶2、脑源性神经营养因子和瞬间受体电位A1而发挥作用。

The smallest degree sum that yields potentially K_(r,r)-graphic sequences

We consider a variation of a classical Turán-type extremal problem as follows: Determine the smallest even integer σ(Kr,r,n) such that every n-term graphic sequence π = (d1,d2,...,dn) with term sum σ(π) = d1 + d2 + ... + dn ≥ σ(Kr,r,n) is potentially Kr,r-graphic, where Kr,r is an r × r complete bipartite graph, i.e. π has a realization G containing Kr,r as its subgraph. In this paper, the values σ(Kr,r,n) for even r and n ≥ 4r2 - r - 6 and for odd r and n ≥ 4r2 + 3r - 8 are determined.

The Smallest Degree Sum That Yields Potentially Kr＋1 - K3-Graphic Sequences

Let a（Kr,＋1 - K3,n） be the smallest even integer such that each n-term graphic sequence п= （d1,d2,…dn） with term sum σ（п） = d1 ＋ d2 ＋…＋ dn 〉 σ（Kr＋1 -K3,n） has a realization containing Kr＋1 - K3 as a subgraph, where Kr＋1 -K3 is a graph obtained from a complete graph Kr＋1 by deleting three edges which form a triangle. In this paper, we determine the value σ（Kr＋1 - K3,n） for r ≥ 3 and n ≥ 3r＋ 5.

On-line Monitoring for Phosphorus Removal Process and Bacterial Community in Sequencing Batch Reactor

For efficient energy consumption and control of effluent quality, the cycle duration for a sequencing batch reactor (SBR) needs to be adjusted by real-time control according to the characteristics and loading of waste-water. In this study, an on-line information system for phosphorus removal processes was established. Based on the analysis for four systems with different ecological community structures and two operation modes, anaerobic-aerobic process and anaerobic-anaerobic process, the characteristic patterns of oxidation-reduction potential (ORP) and pH were related to phosphorous dynamics in the anaerobic, anoxic and aerobic phases, for determination of the end of phosphorous removal. In the operation mode of anaerobic-aerobic process, the pH profile in the anaerobic phase was used to estimate the relative amount of phosphorous accumulating organisms (PAOs) and glycogen accumulat-ing organisms (GAOs), which is beneficial to early detection of ecology community shifts. The on-line sensor val-ues of pH and ORP may be used as the parameters to adjust the duration for phosphorous removal and community shifts to cope with influent variations and maintain appropriate operation conditions.

Knowledge-based potentials in bioinformatics: From a physicist's viewpoint

Biological raw data are growing exponentially, providing a large amount of information on what life is. It is believed that potential functions and the rules governing protein behaviors can be revealed from analysis on known native structures of proteins. Many knowledge-based potentials for proteins have been proposed. Contrary to most existing review articles which mainly describe technical details and applications of various potential models, the main foci for the discussion here are ideas and concepts involving the construction of potentials, including the relation between free energy and energy, the additivity of potentials of mean force and some key issues in potential construction. Sequence analysis is briefly viewed from an energetic viewpoint.

On a Problem of Potentially Pancyclic-graphic Sequences Due to S.B. Rao

A non-increasing sequenceπ=(d_1,d_2,···,d_n)of nonnegative integers is said to be potentially hamiltonian-graphic(resp.potentially pancyclic-graphic)if it is realizable by a simple graph on n vertices containing a hamiltonian cycle(resp.containing cycles of every length from 3 to n).A.R.Rao and S.B.Rao(J.Combin.Theory Ser.B,13(1972),185–191)and Kundu(Discrete Math.,6(1973),367–376)presented a characterization ofπ=(d_1,d_2,···,d_n)that is potentially hamiltonian-graphic.S.B.Rao(Lecture Notes in Math.,No.855,Springer Verlag,1981,417–440,Unsolved Problem 2)further posed the following problem:present a characterization ofπ=(d_1,d_2,···,d_n)that is potentially pancyclic-graphic.In this paper,we first give solution to this problem for the case of 4≤n≤11.Moreover,we also show that a near regular graphic sequenceπ=(d_1,d_2,···,d_n)with dn≥3 is potentially pancyclic-graphic.

The transcriptome analysis of cleft lip/palate-related PTCH1 variants in GMSM-K cells show carcinogenic potential

Cancer progression involves the sonic hedgehog(SHH)pathway,in which the receptor PTCH1 actives the downstream pathways.Dysfunction of PTCH1 can lead to nevoid basal cell carcinoma Syndrome(NBCCs)including neoplastic disease and congenital disorder.To evaluate the relationship between PTCH1 and cancer,we applied the CRISPR/Cas9 system to knock out PTCH1 in oral nontumorous epithelial cells(GMSM-K).Then we screened six PTCH1 variants associated with cleft lip/palate(CL/P),one of the congenital disorders in NBCCs,and generated PTCH1 variant and wild-type recombinant PTCH1^(−/−)GMSM-K cell lines.Transcriptome sequencing was conducted in these cell lines.The results revealed that differentially expressed genes(DEGs)in PTCH1^(−/−)GMSM-K were enriched in extracellular compartments,contributing epithelial diseases by pathway enrichment analysis.RT-PCR confirmed that KRT34,KRT81,KRT86,PDGFB,and WNT10B genes,associated with extracellular compartments were highly expressed in PTCH1^(−/−).The Kyoto Encyclopedia of Genes and Genomes analysis also suggested that DEGs are closely related to focal adhesion,transcriptional misregulation,and proteoglycans in breast and gastric cancers.Comparative analysis of samples revealed that the CL/P-associated PTCH1 variants A443G and V908G are potentially carcinogenic.These findings provide new insights into the carcinogenic potential of PTCH1 dysfunction.

A neutral-point potential balance control strategy for three-level inverter based on VSVPWM

The topology of diode neutral-point-clamped(NPC)three-level inverter is prone to neutral-point potential offset.When the sum of three-phase current is zero,the virtual space vector pulse width modulation(VSVPWM)scheme does not cause the neutral-point voltage offset,but it lacks the ability to balance the deviation.For this reason,a neutral-point potential control strategy combining virtual space vector modulation and loop width control is proposed.The neutral-point potential is balanced by introducing the distribution factor for the regions with redundant vectors.For other regions,the potential is controlled by selecting a suitable switching sequence.Meanwhile,the effect on the virtual vector modulation is reduced within the loop width by setting an appropriate loop width,thereby improving the balance effect.The simulation results show that the proposed method has a strong ability to control the offset and has excellent potential balance performance under the conditions of balanced load,unbalanced load and asymmetric capacitance parameters.

一种适用三电平并联系统的虚拟矢量调制策略

直流侧中点电位平衡和零序环流抑制是保证三电平逆变器并联系统正常运行的重要问题,然而基于冗余小矢量对调节的环流抑制方案进一步增加空间矢量脉宽调制(space vector pulse width modulation,SVPWM)中点电流无法对称消除的场景,加剧双机系统中点电位不平衡程度。该文基于虚拟矢量合成思路,提出一种满足零序环流抑制和中点电位平衡需求的双机虚拟矢量调制方法。该方法重新定义虚拟中矢量,通过缩短中矢量作用时间和增加参与合成的对称冗余小矢量以减少中点电流不平衡场景;分析不同扇区不同控制目标下虚拟矢量合成规则,利用相占空比法优化虚拟矢量的作用顺序,减少矢量合成过程中的开关次数。与传统SVPWM策略下中点电压变化量的对比分析表明,所提方法能够削弱环流抑制和中点电位平衡的耦合关系。最后,利用Starsim平台实验验证该策略的有效性。

The Erds-Jacobson-Lehel conjecture on potentially P_k-graphic sequence is true

A variation in the classical Turn extremal problem is studied. A simple graph G of order n is said to have property P k if it contains a clique of size k+1 as its subgraph. An n term nonincreasing nonnegative integer sequence π=(d 1,d 2,...,d n) is said to be graphic if it is the degree sequence of a simple graph G of order n and such a graph G is referred to as a realization of π . A graphic sequence π is said to be potentially P k graphic if it has a realization G having property P k . The problem: determine the smallest positive even number σ(k,n) such that every n term graphic sequence π=(d 1,d 2,...,d n) without zero terms and with degree sum σ(π)=d 1+d 2+...+d n at least σ(k,n) is potentially P k graphic has been proved positive.

水华对中华绒螯蟹(Eriocheir sinensis)池塘养殖系统细菌群落及潜在致病菌的影响

近年来,水华频发制约着中华绒螯蟹养殖业的发展,环境微生物群落响应水华发生与消退机制是预防与治理水华的重要组成部分。为了解析中华绒螯蟹养殖系统细菌群落对水华的响应特征,采用16S rRNA基因扩增测序技术对拟浮丝藻水华下中华绒螯蟹养殖池塘细菌群落的组成、结构和潜在病原菌的变化进行了研究。结果显示,水体中的优势细菌类群为伯克霍尔德菌科(Burkholderiaceae)和微杆菌科(Microbacteriaaceae),底泥中的优势类群为Steroidobacteraceae菌科和Burkholderiaceae等;科水平下的蓝细菌门类群和其他大部分细菌群落呈显著正相关。养殖水体中潜在病原菌的丰度与水华的生消呈现出相反的趋势,OTU365和OTU1614(均属于Candidatus Similichlamydia epinepheli)是其主要的潜在病原菌,而在底泥中潜在致病菌表现出富集的特征,以OTU1280(大肠杆菌,Escherichia coli)为主。CCA结果显示,蓝藻暴发过程中,Tem、NO3--N、NO2--N及NH4+-N驱使水体蓝藻门细菌类群的变异,NO2--N是驱动养殖水体潜在致病菌群落变异的主导因子。研究结果为防治中华绒螯蟹养殖水华发生提供了宝贵的数据支撑。

基于潜力类型差异化的农村居民点建设用地整治潜力测算及释放——以华南丘陵区某县为例

从其他闲散用地、闲置建设用地及超标准宅基地3个角度对农村居民点建设用地整治潜力进行测算,从经济角度探索区域整治效益,并结合整治潜力、效益确定建设用地整治时序。结果表明,研究区农村居民点建设用地整治潜力充足,其中超标准宅基地整治潜力显著;其他闲散用地及闲置建设用地整治效益优越,超标准宅基地整治收支差异明显;农村居民点建设用地整治潜力释放大小与整治面积、效益、时序相关,由地区能力与需求决定。

A Characterization for a Sequence to be Potentially K_(r+1) — e-graphic

Let n 〉 r, let lr --- （dl,d2,-,dn） be a non-increasing sequence of nonnegative integers and let Kr＋l - e be the graph obtained from Kr＋l by deleting one edge. If zr has a realization G containing Kr＋l - e as a subgraph, then r is said to be potentially Kr＋l - e-graphic. In this paper, we give a characterization for a sequence π to be potentially Kr＋l - e-graphic.

中国潜在植被空间分布格局

潜在植被作为一种与所处立地达到平衡的演替终态,反映的是无人类干扰情况下,立地所能发育形成的最稳定成熟的一种顶极植被类型,是一个地区现状植被的发展趋势。潜在植被的研究能够真实反映气候条件对植被形态变化的影响,是植被-环境分类与关系研究的起点,也是全球变化与陆地生态系统研究的关键。以综合顺序分类法为基本理论基础,在GIS研究方法支持下,利用我国2348个气象台站1961～1990年30a的气候资料,对我国潜在植被类型进行了划分,得出以下结论:(1)分布在我国的潜在植被类型有41类,表现出了我国潜在植被的多样性特征;从面积相对比例来看,我国潜在植被在分布面积上很不均匀,面积最大的寒冷潮湿多雨冻原、高山草甸类分布面积达到1526188km2,面积最小的炎热微干稀树草原类分布面积仅为13km2。(2)海拔0～6800m之间,是我国潜在植被主要分布的海拔界限,并且此海拔段植被多样性随海拔变化呈现出一定的规律性,即随海拔增加植被多样性显著下降。(3)我国潜在植被的空间分布很好的体现了三向地带性规律,而决定这种分布的主要原因是太阳辐射的纬度性变化、距离海洋的远近引起水分条件变化以及由海拔高度引起的从基带向上热量和干湿变化。(4)我国潜在植被空间格局在重心分布上,主要表现为空间聚集(P1、P2、P3、P4、P5、P6)和空间线型邻接(L1、L2、L3)两个主要特征。

准噶尔盆地滴南凸起水西沟群层序地层特征

基于Vail经典层序地层学理论,综合运用地质、测井和地震资料,对准噶尔盆地滴南凸起水西沟群的层序地层特征及勘探潜力进行了研究。根据地震、钻井及测井资料显示的界面特征,识别出5个层序界面、4个初始湖泛面、4个最大湖泛面,据此将研究区侏罗系水西沟群划分为JSQ1、JSQ2、JSQ3J、SQ4 4个三级层序,12个体系域。对各三级层序特征进行了分析,建立了水西沟群八道湾组和三工河组沉积体系分布格架和地层层序模式。据八道湾组一段及三工河组二段一砂层组沉积相平面展布特征及油气显示情况,认为这两个层段油气勘探潜力巨大,勘探的主要方向为与辫状河道砂体有关的岩性圈闭和低幅度背斜圈闭。

论南方海相层系有效供烃能力的主要控制因素

通过对烃源条件和天然气成藏过程的动力学分析,从高有机质丰度海相碎屑岩层段发育、构造沉积类型特征、高—过成熟阶段烃源灶性质演变形成的气源接替效应以及生烃强度与非构造性散失强度(速率)在地质历史中数量关系等若干方面,探讨了影响中国南方海相层系天然气成藏的有效供烃能力的主要地质-地球化学因素。指出有效供烃效率是烃源岩性质、构造-沉积类型、烃源灶类型及其叠合、生烃强度历史演化和天然气散失速率诸控制要素的综合效应。提出烃源条件分级评价指标,并对南方海相层系的有效供烃能力进行了初步评价。

利用伪随机序列相关辨识检测视觉诱发电位

诱发电位在临床诊断及中枢神经系统的研究中有重要意义。目前的诱发电位检测方法是平均法，由平均法获得的诱发电位远不能满足许多实际应用的需要。本文利用伪随机序列相关辨识技术，实现了快速检测视觉诱发电位。文中对这项技术的原理作了详细的描述，给出了检测算法，并分析了检测信噪比相对平均法的改善情况。实验结果显示，这项技术能大大缩短检测时间，对克服时变因素的影响，减少病人的痛苦，以及对诱发电位在神经外科手术中的应用都有深远意义。

油藏规模序列法在胜坨油区资源评价中的应用

胜坨油区已勘探开发40多年,油气勘探程度高。应用油藏规模序列法对该地区资源量进行了评价,结果表明:胜坨油区仍有124个30×104级地质储量以上的待发现油藏,剩余资源总量达1.46×108t。待发现油藏的规模不仅局限于百万吨级储量以下的小规模油藏,百万吨级储量以上的油藏还有35个,地质储量9672×104t。应用油藏规模序列法所计算的胜坨油区剩余资源量及其规模分布能够为该地区今后的勘探工作提供指导。