To improve the efficiency of fetching and transplanting seedlings for the mechanization of strawberry planting,an integrated transplanting mechanism was designed with protruding,fetching and planting performance to ac...To improve the efficiency of fetching and transplanting seedlings for the mechanization of strawberry planting,an integrated transplanting mechanism was designed with protruding,fetching and planting performance to achieve rapid fetching and pushing bowl movements.According to the working principle of the slewing mechanism,a kinematics model and the optimization goal were established,respectively.Based on visual auxiliary analysis software,optimal parameters were obtained.A three-dimensional model was established to obtain a simulation trajectory by means of a virtual simulation design analysis.Three-dimensional printing technology was used to manufacture the test prototype,and the actual working trajectories of the test prototype were extracted using high-speed photography technology,which verified the consistency of the actual trajectory with the theoretical and simulated trajectories.A prototype transplanting experiment was performed with the success rate of seedling extraction of 91.2%and excellent planting rate of 82.8%,which met the requirements for integrated strawberry harvesting,planting and transplanting.The experimental results verified the correctness and feasibility of the design of integrated transplanting mechanism.展开更多
The title compound 1-(3-amino-[1,2,4]triazol-1-yl)-3,3-dimethyl-butan-2-one(3) was synthesized by Hofmann-alkylation reaction of 1-chloro-3,3-dimethyl-butan-2-one(1) and ~1H-[1,2,4]triazol-3-ylamine(2) with eq...The title compound 1-(3-amino-[1,2,4]triazol-1-yl)-3,3-dimethyl-butan-2-one(3) was synthesized by Hofmann-alkylation reaction of 1-chloro-3,3-dimethyl-butan-2-one(1) and ~1H-[1,2,4]triazol-3-ylamine(2) with equal amount of K_2CO_3 as acid acceptor. The structure of compound 3 was characterized by ~1H NMR, 13 C NMR, HRMS and single-crystal X-ray diffraction. The compound crystallizes in the monoclinic system, space group P21/n with a = 5.7227(8), b = 27.924(4), c = 6.2282(7) ?, β = 101.892(11)°, V = 973.9(2) ?~3, Z = 4, T = 180.00(10) K, μ(MoKα) = 0.087 mm^(-1), Dc = 1.243 g/cm^3, 3832 reflections measured(3.648≤θ≤26.022°), 1916 unique reflections(Rint = 0.0359, Rsigma = 0.0572) used in all calculations. The final R = 0.0557(I 〉 2σ(I)) and w R = 0.1276(all data). Bioassay showed that 3 displayed excellent activity as plant growth regulator with inducing lateral root formation and enhancing primary root elongation at 0.27 mmol/L(50 ppm) in soybeen(He Feng-50). Good water solubility was found with 50 mg in 1 m L of water. Therefore, application of 3 in agriculture is more environmentally friendly due to cosolvent-free condition, and results in improved abiotic-stress tolerance by affecting the root growth. And furthermore, it can be used as a precursor to investigate the function of regulating plant root growth.展开更多
The effects of soil moisture content, pot seedling buried depth, and pot seedling height on the survival rate and growth of dry planted seedlings were researched by field experiment. The results showed that the seedli...The effects of soil moisture content, pot seedling buried depth, and pot seedling height on the survival rate and growth of dry planted seedlings were researched by field experiment. The results showed that the seedling survival rate and growth were enhanced with the increase of soil moisture content after pot seedling transplanting, but there was no significant difference in the survival rate and growth of seedlings with 85% and 100% of soil moisture content. Pot seedling buried depth was one of the important factors affecting the survival rate, and when the pot seedlings were buried 2 cm deep, the seedlings showed high survival rate and excellent growth. Under the same condition of soil moisture content and buried depth, seedlings with pot seedling height of 15 cm had the best survival rate and growth. Therefore, the optimized conditions for dry-cultivated rice seedling planting were soil moisture content of 85%, pot seedling buried depth of 2 cm, and pot seedling height of 15 cm.展开更多
Hibiscus rosa-sinensis, Rosa sp. (miniature roses), Sinningia speciosa, Gerbera hybrida, Kalanchoe blossfeldiana, Hydrangea, Begonia x hiemalis, Calceolaria, Cyclamen persicum and Pelargonium domesticum were grown at ...Hibiscus rosa-sinensis, Rosa sp. (miniature roses), Sinningia speciosa, Gerbera hybrida, Kalanchoe blossfeldiana, Hydrangea, Begonia x hiemalis, Calceolaria, Cyclamen persicum and Pelargonium domesticum were grown at six photon flux densities (85, 130, 170, 215, 255 and 300 μmol·m-2·s-1, PFD) during lighting periods of 20 h·day-1 at three air temperatures (18°C, 21°C and 24°C) in midwinter at latitude 59° north. This corresponded to photosynthetic active radiations (PAR) ranging from 6.1 to 21.6 mol·m-2·day-1. Time until flowering decreased in all species except Cyclamen when the temperature increased from 18°C to 21°C, particularly at lower PFD levels. A further increase in temperature, from 21°C to 24°C, clearly decreased time until flowering in six of the ten tested species. Generally, this represented a reduction in the time until flowering between 20% and 40%. The dry weight of the plants at time of flowering increased up to 170 μmol·m-2·s-1 PFD (12.2 mol·m-2·day-1 PAR) in Hibiscus, miniature rose, Kalanchoe and Pelargonium, while the dry weight reached a maximum at 85 to 130 μmol·m-2·s-1 PFD mol·m-2·day-1 (6.1 to 9.4 mol·m-2·day-1)in the other species. Based on the present results a PAR level of 6 to 8 mol m-2·day-1 is recommended for Calceolaria and Cyclamen, of 8 to 10 mol·m-2·day-1 for Sinningia, Gerbera, Kalanchoe, Hydrangea and Begonia, of 10 to 12 mol·m-2·day-1 for Pelargonium and of 12 to 15 mol·m-2 day-1 for Hibiscus and miniature roses.展开更多
It is demonstrated that (3Z)-nonenal (NON) and (3Z)-hexenal (HEX) are oxidized in a cascade by lipoxygenase (LOX) and hydroperoxide peroxygenase (HP peroxygenase) into (2E)-4-hydroxy-2- nonenal (HNE) and (2E)-4-hydrox...It is demonstrated that (3Z)-nonenal (NON) and (3Z)-hexenal (HEX) are oxidized in a cascade by lipoxygenase (LOX) and hydroperoxide peroxygenase (HP peroxygenase) into (2E)-4-hydroxy-2- nonenal (HNE) and (2E)-4-hydroxy-2-hexenal (HHE), respectively. In turn, HNE inactivates LOX terminating the cascade. The hydroxy-alkenals produced serve to inhibit plant pathogens, which initiated the cascade. In addition to LOX, other unknown oxygenases may be involved in the cascade.展开更多
OBJECTIVE: Anti-asthma herbal medicine intervention (ASHMITM), a combination of three tradi- tional Chinese medicinal herbs developed in our laboratory, has demonstrated efficacy in both mouse models of allergic as...OBJECTIVE: Anti-asthma herbal medicine intervention (ASHMITM), a combination of three tradi- tional Chinese medicinal herbs developed in our laboratory, has demonstrated efficacy in both mouse models of allergic asthma, and a double-blind placebo-controlled clinical trial in patients with asthma. This study was designed to determine if the anti-inflammatory effects of individual herbal constituents of ASHMITM exhibited synergy. METHODS: Effects of ASHMI and its components aqueous extracts of Lingzhi (Ganoderma lucidum), Kushen (Sophora flavescens) and Gancao (Glycyrrhiza uralensis), on Th2 cytokine secretion by murine memory Th2 cells (D10.G4.1) and eotaxin-1 secretion by human lung fibroblast (HLF-1) cells were determined by measuring levels in culture supernatants by enzyme- linked immunosorbent assay. Potential synergistic effects were determined by computing interaction indices from concentration-effect curve parameters. RESULTS: Individual Lingzhi, Kushen and Gancao extracts and ASHMI (the combination of individual extracts) inhibited production of interleukin (IL)-4 and IL-5 by murine memory Th2 cells and eotaxin-1 production by HLF-1 cells. The mean 25%-inhibitory-concentration (IC2s) values (mg/mL) forASHMI, Lingzhi, Kushen and Gancao for IL-4 production were 30.9, 79.4, 123, and 64.6, respectively; for IL-5 production were 30.2, 263, 123.2 and 100, respectively; for eotaxin-1 were 13.2, 16.2, 30.2, and 25.1, respectively. The IC50values (mg/mL) for ASHMI, Lingzhi, Kushen and Gancao for IL-4 production were 158.5, 239.9, 446.7, and 281.8, respectively; for eotaxin-1 were 38.1, 33.1, 100, and 158.5, respectively. The interaction indices of ASHMI constituents at IC25 were 0.35 for IL-4, 0.21 for IL-5 and 0.59 for eotaxin-l. The interaction indices at IC^0 values were 0.50 for IL-4 and 0.62 for eotaxin-1 inhibition. Inhibition of IL-5 did not reach IC^0 values. All interaction indices were below 1 which indicated synergy. CONCLUSION: By comparing the interaction index values, we find that constituents in ASHMITM synergistically inhibited eotaxin-1 production as well as Th2 cytokine production.展开更多
The influence of a 40-fold attenuated geomagnetic field and its combined action with low doses of α- and γ-irradiation on the physiological characteristics of seeds of the highest plants and redox properties of wate...The influence of a 40-fold attenuated geomagnetic field and its combined action with low doses of α- and γ-irradiation on the physiological characteristics of seeds of the highest plants and redox properties of water was investigated. It established the reduction of seed germination both under direct and indirect effects due to water action of attenuated geomagnetic field. A negative effect of hypomagnetic field on grown characteristics of seeds under indirect effect via water was decreased by the low doses of γ-irradiation, and was increased by low doses of α-irradiation, i.e. ionized radiation was the dominant factor in their combined action. It was revealed the increasing of the value of the oxidation-reduction potential of water under the influence of low-intensive α-ir-radiation (239Pu), γ-irradiation (137Cs) and also that the magnetic induction attenuated pointing to a natural decline. The increasing of the oxidation-reduction potential value testifies about “the regular decreasing of internal energy of water molecules” and the increasing of its oxidative properties, which, in our opinion, is caused the inhibition of the germination of seeds. It is supposed that namely water is the main component in the effects of studying factors on bio-objects, which acts due to the alterations of the properties and structural content of water.展开更多
基金Supported by the National Natural Science Foundation of China Youth Fund Project(52005221)"the 13th Five-Year"National Key Research and Development Program:High-speed Planting Technology and Equipment Research and Development(2017YFD0700800)。
文摘To improve the efficiency of fetching and transplanting seedlings for the mechanization of strawberry planting,an integrated transplanting mechanism was designed with protruding,fetching and planting performance to achieve rapid fetching and pushing bowl movements.According to the working principle of the slewing mechanism,a kinematics model and the optimization goal were established,respectively.Based on visual auxiliary analysis software,optimal parameters were obtained.A three-dimensional model was established to obtain a simulation trajectory by means of a virtual simulation design analysis.Three-dimensional printing technology was used to manufacture the test prototype,and the actual working trajectories of the test prototype were extracted using high-speed photography technology,which verified the consistency of the actual trajectory with the theoretical and simulated trajectories.A prototype transplanting experiment was performed with the success rate of seedling extraction of 91.2%and excellent planting rate of 82.8%,which met the requirements for integrated strawberry harvesting,planting and transplanting.The experimental results verified the correctness and feasibility of the design of integrated transplanting mechanism.
基金supported by the National Natural Science Foundation of China(No.2012BAD20B04)
文摘The title compound 1-(3-amino-[1,2,4]triazol-1-yl)-3,3-dimethyl-butan-2-one(3) was synthesized by Hofmann-alkylation reaction of 1-chloro-3,3-dimethyl-butan-2-one(1) and ~1H-[1,2,4]triazol-3-ylamine(2) with equal amount of K_2CO_3 as acid acceptor. The structure of compound 3 was characterized by ~1H NMR, 13 C NMR, HRMS and single-crystal X-ray diffraction. The compound crystallizes in the monoclinic system, space group P21/n with a = 5.7227(8), b = 27.924(4), c = 6.2282(7) ?, β = 101.892(11)°, V = 973.9(2) ?~3, Z = 4, T = 180.00(10) K, μ(MoKα) = 0.087 mm^(-1), Dc = 1.243 g/cm^3, 3832 reflections measured(3.648≤θ≤26.022°), 1916 unique reflections(Rint = 0.0359, Rsigma = 0.0572) used in all calculations. The final R = 0.0557(I 〉 2σ(I)) and w R = 0.1276(all data). Bioassay showed that 3 displayed excellent activity as plant growth regulator with inducing lateral root formation and enhancing primary root elongation at 0.27 mmol/L(50 ppm) in soybeen(He Feng-50). Good water solubility was found with 50 mg in 1 m L of water. Therefore, application of 3 in agriculture is more environmentally friendly due to cosolvent-free condition, and results in improved abiotic-stress tolerance by affecting the root growth. And furthermore, it can be used as a precursor to investigate the function of regulating plant root growth.
基金Supported by the Key Research and Development Project of Shandong Province(2016GNC111005&2015GNC111019)
文摘The effects of soil moisture content, pot seedling buried depth, and pot seedling height on the survival rate and growth of dry planted seedlings were researched by field experiment. The results showed that the seedling survival rate and growth were enhanced with the increase of soil moisture content after pot seedling transplanting, but there was no significant difference in the survival rate and growth of seedlings with 85% and 100% of soil moisture content. Pot seedling buried depth was one of the important factors affecting the survival rate, and when the pot seedlings were buried 2 cm deep, the seedlings showed high survival rate and excellent growth. Under the same condition of soil moisture content and buried depth, seedlings with pot seedling height of 15 cm had the best survival rate and growth. Therefore, the optimized conditions for dry-cultivated rice seedling planting were soil moisture content of 85%, pot seedling buried depth of 2 cm, and pot seedling height of 15 cm.
基金funded by the Norwegian Research Council and the Norwegian Growers Association
文摘Hibiscus rosa-sinensis, Rosa sp. (miniature roses), Sinningia speciosa, Gerbera hybrida, Kalanchoe blossfeldiana, Hydrangea, Begonia x hiemalis, Calceolaria, Cyclamen persicum and Pelargonium domesticum were grown at six photon flux densities (85, 130, 170, 215, 255 and 300 μmol·m-2·s-1, PFD) during lighting periods of 20 h·day-1 at three air temperatures (18°C, 21°C and 24°C) in midwinter at latitude 59° north. This corresponded to photosynthetic active radiations (PAR) ranging from 6.1 to 21.6 mol·m-2·day-1. Time until flowering decreased in all species except Cyclamen when the temperature increased from 18°C to 21°C, particularly at lower PFD levels. A further increase in temperature, from 21°C to 24°C, clearly decreased time until flowering in six of the ten tested species. Generally, this represented a reduction in the time until flowering between 20% and 40%. The dry weight of the plants at time of flowering increased up to 170 μmol·m-2·s-1 PFD (12.2 mol·m-2·day-1 PAR) in Hibiscus, miniature rose, Kalanchoe and Pelargonium, while the dry weight reached a maximum at 85 to 130 μmol·m-2·s-1 PFD mol·m-2·day-1 (6.1 to 9.4 mol·m-2·day-1)in the other species. Based on the present results a PAR level of 6 to 8 mol m-2·day-1 is recommended for Calceolaria and Cyclamen, of 8 to 10 mol·m-2·day-1 for Sinningia, Gerbera, Kalanchoe, Hydrangea and Begonia, of 10 to 12 mol·m-2·day-1 for Pelargonium and of 12 to 15 mol·m-2 day-1 for Hibiscus and miniature roses.
文摘It is demonstrated that (3Z)-nonenal (NON) and (3Z)-hexenal (HEX) are oxidized in a cascade by lipoxygenase (LOX) and hydroperoxide peroxygenase (HP peroxygenase) into (2E)-4-hydroxy-2- nonenal (HNE) and (2E)-4-hydroxy-2-hexenal (HHE), respectively. In turn, HNE inactivates LOX terminating the cascade. The hydroxy-alkenals produced serve to inhibit plant pathogens, which initiated the cascade. In addition to LOX, other unknown oxygenases may be involved in the cascade.
基金supported by NIH/NCCAM center grant # 1P01 AT002644725-01"Center for Chinese Herbal Therapy(CHT) for Asthma"to Dr.Xiu-Min Li
文摘OBJECTIVE: Anti-asthma herbal medicine intervention (ASHMITM), a combination of three tradi- tional Chinese medicinal herbs developed in our laboratory, has demonstrated efficacy in both mouse models of allergic asthma, and a double-blind placebo-controlled clinical trial in patients with asthma. This study was designed to determine if the anti-inflammatory effects of individual herbal constituents of ASHMITM exhibited synergy. METHODS: Effects of ASHMI and its components aqueous extracts of Lingzhi (Ganoderma lucidum), Kushen (Sophora flavescens) and Gancao (Glycyrrhiza uralensis), on Th2 cytokine secretion by murine memory Th2 cells (D10.G4.1) and eotaxin-1 secretion by human lung fibroblast (HLF-1) cells were determined by measuring levels in culture supernatants by enzyme- linked immunosorbent assay. Potential synergistic effects were determined by computing interaction indices from concentration-effect curve parameters. RESULTS: Individual Lingzhi, Kushen and Gancao extracts and ASHMI (the combination of individual extracts) inhibited production of interleukin (IL)-4 and IL-5 by murine memory Th2 cells and eotaxin-1 production by HLF-1 cells. The mean 25%-inhibitory-concentration (IC2s) values (mg/mL) forASHMI, Lingzhi, Kushen and Gancao for IL-4 production were 30.9, 79.4, 123, and 64.6, respectively; for IL-5 production were 30.2, 263, 123.2 and 100, respectively; for eotaxin-1 were 13.2, 16.2, 30.2, and 25.1, respectively. The IC50values (mg/mL) for ASHMI, Lingzhi, Kushen and Gancao for IL-4 production were 158.5, 239.9, 446.7, and 281.8, respectively; for eotaxin-1 were 38.1, 33.1, 100, and 158.5, respectively. The interaction indices of ASHMI constituents at IC25 were 0.35 for IL-4, 0.21 for IL-5 and 0.59 for eotaxin-l. The interaction indices at IC^0 values were 0.50 for IL-4 and 0.62 for eotaxin-1 inhibition. Inhibition of IL-5 did not reach IC^0 values. All interaction indices were below 1 which indicated synergy. CONCLUSION: By comparing the interaction index values, we find that constituents in ASHMITM synergistically inhibited eotaxin-1 production as well as Th2 cytokine production.
文摘The influence of a 40-fold attenuated geomagnetic field and its combined action with low doses of α- and γ-irradiation on the physiological characteristics of seeds of the highest plants and redox properties of water was investigated. It established the reduction of seed germination both under direct and indirect effects due to water action of attenuated geomagnetic field. A negative effect of hypomagnetic field on grown characteristics of seeds under indirect effect via water was decreased by the low doses of γ-irradiation, and was increased by low doses of α-irradiation, i.e. ionized radiation was the dominant factor in their combined action. It was revealed the increasing of the value of the oxidation-reduction potential of water under the influence of low-intensive α-ir-radiation (239Pu), γ-irradiation (137Cs) and also that the magnetic induction attenuated pointing to a natural decline. The increasing of the oxidation-reduction potential value testifies about “the regular decreasing of internal energy of water molecules” and the increasing of its oxidative properties, which, in our opinion, is caused the inhibition of the germination of seeds. It is supposed that namely water is the main component in the effects of studying factors on bio-objects, which acts due to the alterations of the properties and structural content of water.