Currently,artificial-membrane lungs consist of thousands of hollow fiber membranes where blood flows around the fibers and gas flows inside the fibers,achieving diffusive gas exchange.At both ends of the fibers,the in...Currently,artificial-membrane lungs consist of thousands of hollow fiber membranes where blood flows around the fibers and gas flows inside the fibers,achieving diffusive gas exchange.At both ends of the fibers,the interspaces between the hollow fiber membranes and the plastic housing are filled with glue to separate the gas from the blood phase.During a uniaxial centrifugation process,the glue forms the“potting.”The shape of the cured potting is then determined by the centrifugation process,limiting design possibilities and leading to unfavorable stagnation zones associated with blood clotting.In this study,a new multiaxial centrifugation process was developed,expanding the possible shapes of the potting and allowing for completely new module designs with potentially superior blood flow guidance within the potting margins.Two-phase simulations of the process in conceptual artificial lungs were performed to explore the possibilities of a biaxial centrifugation process and determine suitable parameter sets.A corresponding biaxial centrifugation setup was built to prove feasibility and experimentally validate four conceptual designs,resulting in good agreement with the simulations.In summary,this study shows the feasibility of a multiaxial centrifugation process allowing greater variety in potting shapes,eliminating inefficient stagnation zones and more favorable blood flow conditions in artificial lungs.展开更多
Spruce (Picea Smithiana) is an evergreen tree and distributed through the temperate Himalaya at the elevation of 2000 - 3500 m. Natural regeneration of spruce (Picea smithiana) is generally slow and almost negligible ...Spruce (Picea Smithiana) is an evergreen tree and distributed through the temperate Himalaya at the elevation of 2000 - 3500 m. Natural regeneration of spruce (Picea smithiana) is generally slow and almost negligible due to a number of factors e.g. presence of un decomposed raw humus on forest floor low germinative capacity of seed and infrequent good seed years. In order to supplement natural regeneration through artificial means, nursery raising assumes significance. The present study aimed at optimizing the root trainer container size and the potting mixture ingredients and their proportion for the production of quality nursery stock of Picea smithiana. Nine combinations of potting mixture ingredients (Forest Soil, Garden Soil, Sand, FYM and Lake weed) were tested in various ratios for the optimization of potty mixture in different root trainer sizes. Simultaneously, three different volumes (150cc, 250cc and 300cc) of root trainers were tested for suitability of container size. Chemical and physical analysis of potting mixture ingredients was carried out for better understanding of nutrient status. The best seedling growth at seven month age in terms of height (5.53 cm), Collar diameter (1.57 mm), fresh shoot weight (0.34 g), dry shoot weight (0.10 g), fresh root weight (0.27 g) , dry root weight (0.03 g), root shoot ratio(2:52), and total fresh biomass (0.61 g) were observed when seedlings were raised in root trainer container size (300cc) filled with potting mixture of (M8) Soil:Sand:FYM (1:1:2) and the lowest growth parameters were observed in root trainer container size (150cc) filled with forest soil only. Plantable quality seedlings can be recovered within a period of 7 months using potting mixture of Soil, Sand and FYM (1:1:2) and Soil, Sand and Lake weed (1:1:2) ratios in root trainer container size 300cc.展开更多
为获得高层建筑围护结构设计风荷载,通常需要考虑其表面风压系数的概率特征,进而进行极值估计。针对当前基于超越阈值模型的风压系数极值估计方法存在阈值选取困难,需要较大样本的不足,基于高层建筑标准模型进行风洞试验,首先研究其表...为获得高层建筑围护结构设计风荷载,通常需要考虑其表面风压系数的概率特征,进而进行极值估计。针对当前基于超越阈值模型的风压系数极值估计方法存在阈值选取困难,需要较大样本的不足,基于高层建筑标准模型进行风洞试验,首先研究其表面风压系数的概率特征,结果表明迎风区测点接近高斯分布,分离区测点风压系数母体接近Gamma分布,风压系数极小值接近GEV(general extreme value,GEV)分布;提出一种改进的POT(peak over threshold,POT)极值估计方法进行表面风压系数极值估计,进而与几种传统极值估计方法进行对比,结果表明改进POT极值估计方法可实现小样本的风压系数极值估计,其估计结果与大样本容量的标准极值偏差小于5%,且稳定性较好;最后给出了标准高层建筑模型表面极值风压系数。展开更多
Deformation can directly reflect the working behavior of the dam,so determining the deformation monitoring control value can effectively monitor the safety of dam operation.The traditional dam deformation monitoring c...Deformation can directly reflect the working behavior of the dam,so determining the deformation monitoring control value can effectively monitor the safety of dam operation.The traditional dam deformation monitoring control value only considers the single measuring point.In order to overcome the limitation,this paper presents a new method to determine the monitoring control value for concrete gravity dam based on the deformations of multi-measuring points.A dam’s comprehensive deformation displacement is determined by the measured values at different measuring points on the positive inverted vertical line and the corresponding weight of eachmeasuring point.The projection pursuit method(PPM)combined with the grey wolf optimization(GWO)algorithm is used to determine the weight of each measuring point according to the spatial correlation distribution characteristics of dam deformation.The peaks over threshold(POT)model based on the extreme value theory is adopted to determine the monitoring control value with the obtained dam comprehensive deformation displacement.In addition,the POTmodel is improved with the automatic threshold determinationmethod based on the 3σcriterion in probability theory and the GWO algorithm,which can avoid subjectivity and randomness in determining the threshold.The results of the engineering application show the feasibility and applicability of the proposed method.展开更多
To improve the efficiency of fetching and transplanting seedlings for the mechanization of strawberry planting,an integrated transplanting mechanism was designed with protruding,fetching and planting performance to ac...To improve the efficiency of fetching and transplanting seedlings for the mechanization of strawberry planting,an integrated transplanting mechanism was designed with protruding,fetching and planting performance to achieve rapid fetching and pushing bowl movements.According to the working principle of the slewing mechanism,a kinematics model and the optimization goal were established,respectively.Based on visual auxiliary analysis software,optimal parameters were obtained.A three-dimensional model was established to obtain a simulation trajectory by means of a virtual simulation design analysis.Three-dimensional printing technology was used to manufacture the test prototype,and the actual working trajectories of the test prototype were extracted using high-speed photography technology,which verified the consistency of the actual trajectory with the theoretical and simulated trajectories.A prototype transplanting experiment was performed with the success rate of seedling extraction of 91.2%and excellent planting rate of 82.8%,which met the requirements for integrated strawberry harvesting,planting and transplanting.The experimental results verified the correctness and feasibility of the design of integrated transplanting mechanism.展开更多
The objective of this study was to determine the role of SLC15A4 in the muramyl dipeptide(MDP)-mediated inflammatory response of bovine rumen epithelial cells(BRECs).First,changes in the m RNA expression of proinflamm...The objective of this study was to determine the role of SLC15A4 in the muramyl dipeptide(MDP)-mediated inflammatory response of bovine rumen epithelial cells(BRECs).First,changes in the m RNA expression of proinflammatory factor genes in BRECs following 10μg m L^(–1)MDP treatments were examined.RT-q PCR results showed that the expression of pro-inflammatory factor(IL-1β,IL-6,and TNF-α)m RNAs were significantly increased under MDP stimulation(P<0.001).Moreover,SLC15A4-Knockout(SLC15A4-KO)cells were obtained through lentivirus packaging,transfection,screening,and cell monoclonal culture.In order to gain further insight into the potential function of SLC15A4,we utilized transcriptome data,which revealed a change in the genes between WT-BRECs and SLC15A4-KO.Five down-regulated pro-inflammatory genes and 13 down-regulated chemokine genes related to the inflammatory response were identified.Meanwhile,the down-regulated genes were mostly enriched in the nuclear factorκB(NF-κB)and mitogen-activated protein kinase(MAPK)signaling pathways.The results of RT-q PCR also verified these detected changes.To further determine the mechanism of how WT and SLC15A4-KO BRECs are involved in inflammatory responses,we investigated the inflammatory responses of cells exposed to MDP.WT-BRECs and SLC15A4-KO were treated with a culture medium containing 10μg m L^(–1)MDP,in comparison to a control without MDP.Our results show that SLC15A4-KO BRECs had reduced the expression of genes(IL-6,TNF-α,CXCL2,CXCL3,CXCL9,and CCL2)and proteins(p-p65 and p-p44/42)from the MDP-mediated inflammatory response compared to WT-BRECs(P<0.05).In this experiment,CRISPR-Cas9 was used to KO the di/tripeptide transporter SLC15A4,and its role was confirmed via the MDP-induced inflammatory response in BRECs.This work will provide a theoretical basis for studying the pro-inflammatory mechanism of MDP and its application in the prevention and treatment of subacute rumen acidosis in dairy cows.展开更多
Introduction and Objectives: In patients with Post-Acute Sequelae of Coronavirus 2 infection (PASC), a post infectious autonomic dysfunction may be one of the underlying mechanisms. Patients often present with exercis...Introduction and Objectives: In patients with Post-Acute Sequelae of Coronavirus 2 infection (PASC), a post infectious autonomic dysfunction may be one of the underlying mechanisms. Patients often present with exercise intolerance and exaggerated heart rate response to exercise. We report a single centre experience of patients with PACS and suspected autonomic dysfunction. Methods: Forty-two patients evaluated in the Outpatient Cardiology Department with suspected PASC were included in the study. Patients complained of compromised exercise performance persisting >3 months after recovery from COVID-19 infection, compared to the pre-COVID-19 period. The patients were evaluated with 12-lead electrocardiogram, echocardiography, 24-hour ECG ambulatory monitoring and either exercise stress test or a 6-minute walk test. Results: All 42 patients demonstrated an exaggerated chronotropic response, defined as the inappropriate increase in heart rate before the 6th minute of exercise >100% of the age-predicted maximal heart rate value with reproduction of clinical symptoms. In addition, 24-hour ambulatory electrocardiography revealed an increased mean heart rate of 92 beats/minute and decreased mean standard deviation of sequential 5-minute N-N interval (SDNN) of 74.4 ms. Pharmaceutical treatment with b-blockers, ivabradine or both was administrated in 29 (69%) resulting in symptomatic improvement in 82.8% of those under treatment. However, residual symptoms persisted in 69% of patients after 3 months. Conclusions: In patients with “Post-acute COVID-19” syndrome, we found an excessive chronotropic response to exercise suggesting autonomic dysfunction as the underlying mechanism of symptoms. Treatment with beta blockers or ivabradine resulted in clinical improvement but a substantial proportion of patients remained symptomatic.展开更多
The objective of this work was to elaborate ceramic water filters from Kaolinite (Cameroon) clay for the elimination of suspended particles from domestic drinking water. In Sub-Sahara Africa and in Cameroon in particu...The objective of this work was to elaborate ceramic water filters from Kaolinite (Cameroon) clay for the elimination of suspended particles from domestic drinking water. In Sub-Sahara Africa and in Cameroon in particular health issues have been linked to the consummation of domestic tap water of high turbidity values both in the rural and urban areas. In order to remedy these problems, ceramic water pot filters have been elaborated in a pilot scale unit with aim of putting in place a unit production. The chemical composition, the thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC) analyses of the raw materials (clay and rice husks) was determined. The crystal phases and scanning electron microscope of Wack clay was also determined. The ceramic pot filter membranes were fabricated from the formulations 70/20/10 of clay/porogen/chamotte respectively with the particle size of the raw material less than or equal to 500 μm. The formulated ceramic pot filters were then sintered at 900˚C in a furnace. These ceramic pot filters were characterized by determining their porosity, withdrawal percentages, water permeability, mechanical and chemical resistance. The study of the efficiency consisted in evaluating the retention rate and permeate flux with respect to time (days) with synthetic water suspensions of turbidity 100 NTU and particle size of 2 μm. The ceramic pot filters were made aiming at studying the efficiency after physical defouling of filters. Physical defouling consisted in brushing the inner surface of the ceramic pot filters with water and drying them at ambient temperature after being used for 11 days and reusing them under the same initial conditions. The produced ceramic pot filter had a volume of 4 L, an average porosity of 36.15%, shrinkage in mass or withdrawal percentage of 18.23%, a water permeability of 59.6 × 10<sup>3</sup> L∙h<sup>−2</sup>∙m<sup>−</sup><sup>2</sup>, mechanical resistance of 6.8 MPa and corrosion resistance of 1.6% in acidic medium and 0.8% in alkaline medium. The evaluation of the retention efficiency reveals that the retention rate of 99.9% was obtained from the 9<sup>th</sup> day of filtration reducing the turbidity value from 100 NTU to less than 0.1 NTU. From the filtration test carried out during the 11 consecutive days, the flow rate varied between 1.46 L∙h<sup>−1</sup> to 2.63 L∙h<sup>−1</sup>. Similar results of retention and flow rate were obtained after physical defouling of the ceramic pot filter membranes and re-using for 11 consecutive days, showing the efficiency of the ceramic pot filter membranes in eliminating suspended particles from drinking water. Cost evaluation for the production unit reveals a total cost of production for 50 ceramic pot filters of 1593.6 USD consisting of fixed assets and variable assets. An estimated selling price of 3.3 USD was obtained which is affordable for both the urban and rural population in Cameroon and in sub-Saharan Africa.展开更多
The objective of this work was to elaborate ceramic water filters from Kaolinite (Cameroon) clay for the elimination of suspended particles from domestic drinking water. In Sub-Sahara Africa and in Cameroon in particu...The objective of this work was to elaborate ceramic water filters from Kaolinite (Cameroon) clay for the elimination of suspended particles from domestic drinking water. In Sub-Sahara Africa and in Cameroon in particular health issues have been linked to the consummation of domestic tap water of high turbidity values both in the rural and urban areas. In order to remedy these problems, ceramic water pot filters have been elaborated in a pilot scale unit with aim of putting in place a unit production. The chemical composition, the thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC) analyses of the raw materials (clay and rice husks) was determined. The crystal phases and scanning electron microscope of Wack clay was also determined. The ceramic pot filter membranes were fabricated from the formulations 70/20/10 of clay/porogen/chamotte respectively with the particle size of the raw material less than or equal to 500 μm. The formulated ceramic pot filters were then sintered at 900˚C in a furnace. These ceramic pot filters were characterized by determining their porosity, withdrawal percentages, water permeability, mechanical and chemical resistance. The study of the efficiency consisted in evaluating the retention rate and permeate flux with respect to time (days) with synthetic water suspensions of turbidity 100 NTU and particle size of 2 μm. The ceramic pot filters were made aiming at studying the efficiency after physical defouling of filters. Physical defouling consisted in brushing the inner surface of the ceramic pot filters with water and drying them at ambient temperature after being used for 11 days and reusing them under the same initial conditions. The produced ceramic pot filter had a volume of 4 L, an average porosity of 36.15%, shrinkage in mass or withdrawal percentage of 18.23%, a water permeability of 59.6 × 10<sup>3</sup> L∙h<sup>−2</sup>∙m<sup>−</sup><sup>2</sup>, mechanical resistance of 6.8 MPa and corrosion resistance of 1.6% in acidic medium and 0.8% in alkaline medium. The evaluation of the retention efficiency reveals that the retention rate of 99.9% was obtained from the 9<sup>th</sup> day of filtration reducing the turbidity value from 100 NTU to less than 0.1 NTU. From the filtration test carried out during the 11 consecutive days, the flow rate varied between 1.46 L∙h<sup>−1</sup> to 2.63 L∙h<sup>−1</sup>. Similar results of retention and flow rate were obtained after physical defouling of the ceramic pot filter membranes and re-using for 11 consecutive days, showing the efficiency of the ceramic pot filter membranes in eliminating suspended particles from drinking water. Cost evaluation for the production unit reveals a total cost of production for 50 ceramic pot filters of 1593.6 USD consisting of fixed assets and variable assets. An estimated selling price of 3.3 USD was obtained which is affordable for both the urban and rural population in Cameroon and in sub-Saharan Africa.展开更多
The People’s Army of Vietnam (PAVN) decided to use conventional warfare to unify Vietnam. The United States military operation against the Ho Chi Minh Trail traffic began in 1959. The (PAVN) connected a series of old...The People’s Army of Vietnam (PAVN) decided to use conventional warfare to unify Vietnam. The United States military operation against the Ho Chi Minh Trail traffic began in 1959. The (PAVN) connected a series of old trails leading from North Vietnam to South Vietnam via Laos and Cambodia. In the 1960s the network of trails was expanded. Trail traffic was interdicted by repeated CIA (Air America) and US Air Force (Operation Ranch Hand) tactical herbicide spraying and bombing missions. During the late 1960s, the Khmer Rouge Army slowly grew in eastern Cambodia during a time when America was spraying and bombing the Ho Chi Minh Trail. On February 22, 1969, the PAVN launched a new offensive against American forces in South Vietnam from their sanctuaries in Cambodia. President Richard Nixon and Dr. Henry Kissinger, decided to spray and bomb Cambodia, a neutral country, to eliminate the PAVN sanctuary base camps. The damage and loss of life as a result of U.S. air campaign resulted in the insurgency (Khmer Rouge) being able to recruit civilian members. This created a dual effect of strengthening the popularity of the Khmer Rouge, led by Pol Pot, enabling him to overthrow the Khmer Republic in 1975. The primary objectives of this study are to determine the political impacts and consequences of: 1) the 1959 United States secret war on the Ho Chi Minh Trail in Cambodia and 2) the 1969 President Nixon’s decision to destroy the PAVN bases hidden in the Cambodian jungles. These United States secret wars in Cambodia had long-lasting effects on Cambodian political, social, and economic stability.展开更多
文摘Currently,artificial-membrane lungs consist of thousands of hollow fiber membranes where blood flows around the fibers and gas flows inside the fibers,achieving diffusive gas exchange.At both ends of the fibers,the interspaces between the hollow fiber membranes and the plastic housing are filled with glue to separate the gas from the blood phase.During a uniaxial centrifugation process,the glue forms the“potting.”The shape of the cured potting is then determined by the centrifugation process,limiting design possibilities and leading to unfavorable stagnation zones associated with blood clotting.In this study,a new multiaxial centrifugation process was developed,expanding the possible shapes of the potting and allowing for completely new module designs with potentially superior blood flow guidance within the potting margins.Two-phase simulations of the process in conceptual artificial lungs were performed to explore the possibilities of a biaxial centrifugation process and determine suitable parameter sets.A corresponding biaxial centrifugation setup was built to prove feasibility and experimentally validate four conceptual designs,resulting in good agreement with the simulations.In summary,this study shows the feasibility of a multiaxial centrifugation process allowing greater variety in potting shapes,eliminating inefficient stagnation zones and more favorable blood flow conditions in artificial lungs.
文摘Spruce (Picea Smithiana) is an evergreen tree and distributed through the temperate Himalaya at the elevation of 2000 - 3500 m. Natural regeneration of spruce (Picea smithiana) is generally slow and almost negligible due to a number of factors e.g. presence of un decomposed raw humus on forest floor low germinative capacity of seed and infrequent good seed years. In order to supplement natural regeneration through artificial means, nursery raising assumes significance. The present study aimed at optimizing the root trainer container size and the potting mixture ingredients and their proportion for the production of quality nursery stock of Picea smithiana. Nine combinations of potting mixture ingredients (Forest Soil, Garden Soil, Sand, FYM and Lake weed) were tested in various ratios for the optimization of potty mixture in different root trainer sizes. Simultaneously, three different volumes (150cc, 250cc and 300cc) of root trainers were tested for suitability of container size. Chemical and physical analysis of potting mixture ingredients was carried out for better understanding of nutrient status. The best seedling growth at seven month age in terms of height (5.53 cm), Collar diameter (1.57 mm), fresh shoot weight (0.34 g), dry shoot weight (0.10 g), fresh root weight (0.27 g) , dry root weight (0.03 g), root shoot ratio(2:52), and total fresh biomass (0.61 g) were observed when seedlings were raised in root trainer container size (300cc) filled with potting mixture of (M8) Soil:Sand:FYM (1:1:2) and the lowest growth parameters were observed in root trainer container size (150cc) filled with forest soil only. Plantable quality seedlings can be recovered within a period of 7 months using potting mixture of Soil, Sand and FYM (1:1:2) and Soil, Sand and Lake weed (1:1:2) ratios in root trainer container size 300cc.
文摘为获得高层建筑围护结构设计风荷载,通常需要考虑其表面风压系数的概率特征,进而进行极值估计。针对当前基于超越阈值模型的风压系数极值估计方法存在阈值选取困难,需要较大样本的不足,基于高层建筑标准模型进行风洞试验,首先研究其表面风压系数的概率特征,结果表明迎风区测点接近高斯分布,分离区测点风压系数母体接近Gamma分布,风压系数极小值接近GEV(general extreme value,GEV)分布;提出一种改进的POT(peak over threshold,POT)极值估计方法进行表面风压系数极值估计,进而与几种传统极值估计方法进行对比,结果表明改进POT极值估计方法可实现小样本的风压系数极值估计,其估计结果与大样本容量的标准极值偏差小于5%,且稳定性较好;最后给出了标准高层建筑模型表面极值风压系数。
文摘Deformation can directly reflect the working behavior of the dam,so determining the deformation monitoring control value can effectively monitor the safety of dam operation.The traditional dam deformation monitoring control value only considers the single measuring point.In order to overcome the limitation,this paper presents a new method to determine the monitoring control value for concrete gravity dam based on the deformations of multi-measuring points.A dam’s comprehensive deformation displacement is determined by the measured values at different measuring points on the positive inverted vertical line and the corresponding weight of eachmeasuring point.The projection pursuit method(PPM)combined with the grey wolf optimization(GWO)algorithm is used to determine the weight of each measuring point according to the spatial correlation distribution characteristics of dam deformation.The peaks over threshold(POT)model based on the extreme value theory is adopted to determine the monitoring control value with the obtained dam comprehensive deformation displacement.In addition,the POTmodel is improved with the automatic threshold determinationmethod based on the 3σcriterion in probability theory and the GWO algorithm,which can avoid subjectivity and randomness in determining the threshold.The results of the engineering application show the feasibility and applicability of the proposed method.
基金Supported by the National Natural Science Foundation of China Youth Fund Project(52005221)"the 13th Five-Year"National Key Research and Development Program:High-speed Planting Technology and Equipment Research and Development(2017YFD0700800)。
文摘To improve the efficiency of fetching and transplanting seedlings for the mechanization of strawberry planting,an integrated transplanting mechanism was designed with protruding,fetching and planting performance to achieve rapid fetching and pushing bowl movements.According to the working principle of the slewing mechanism,a kinematics model and the optimization goal were established,respectively.Based on visual auxiliary analysis software,optimal parameters were obtained.A three-dimensional model was established to obtain a simulation trajectory by means of a virtual simulation design analysis.Three-dimensional printing technology was used to manufacture the test prototype,and the actual working trajectories of the test prototype were extracted using high-speed photography technology,which verified the consistency of the actual trajectory with the theoretical and simulated trajectories.A prototype transplanting experiment was performed with the success rate of seedling extraction of 91.2%and excellent planting rate of 82.8%,which met the requirements for integrated strawberry harvesting,planting and transplanting.The experimental results verified the correctness and feasibility of the design of integrated transplanting mechanism.
基金the National Natural Science Foundation of China(31972589)the earmarked fund for China Agriculture Research System(CARS-36)the Postgraduate Research&Practice Innovation Program of Jiangsu Province,China(KYCX21-3283)。
文摘The objective of this study was to determine the role of SLC15A4 in the muramyl dipeptide(MDP)-mediated inflammatory response of bovine rumen epithelial cells(BRECs).First,changes in the m RNA expression of proinflammatory factor genes in BRECs following 10μg m L^(–1)MDP treatments were examined.RT-q PCR results showed that the expression of pro-inflammatory factor(IL-1β,IL-6,and TNF-α)m RNAs were significantly increased under MDP stimulation(P<0.001).Moreover,SLC15A4-Knockout(SLC15A4-KO)cells were obtained through lentivirus packaging,transfection,screening,and cell monoclonal culture.In order to gain further insight into the potential function of SLC15A4,we utilized transcriptome data,which revealed a change in the genes between WT-BRECs and SLC15A4-KO.Five down-regulated pro-inflammatory genes and 13 down-regulated chemokine genes related to the inflammatory response were identified.Meanwhile,the down-regulated genes were mostly enriched in the nuclear factorκB(NF-κB)and mitogen-activated protein kinase(MAPK)signaling pathways.The results of RT-q PCR also verified these detected changes.To further determine the mechanism of how WT and SLC15A4-KO BRECs are involved in inflammatory responses,we investigated the inflammatory responses of cells exposed to MDP.WT-BRECs and SLC15A4-KO were treated with a culture medium containing 10μg m L^(–1)MDP,in comparison to a control without MDP.Our results show that SLC15A4-KO BRECs had reduced the expression of genes(IL-6,TNF-α,CXCL2,CXCL3,CXCL9,and CCL2)and proteins(p-p65 and p-p44/42)from the MDP-mediated inflammatory response compared to WT-BRECs(P<0.05).In this experiment,CRISPR-Cas9 was used to KO the di/tripeptide transporter SLC15A4,and its role was confirmed via the MDP-induced inflammatory response in BRECs.This work will provide a theoretical basis for studying the pro-inflammatory mechanism of MDP and its application in the prevention and treatment of subacute rumen acidosis in dairy cows.
文摘Introduction and Objectives: In patients with Post-Acute Sequelae of Coronavirus 2 infection (PASC), a post infectious autonomic dysfunction may be one of the underlying mechanisms. Patients often present with exercise intolerance and exaggerated heart rate response to exercise. We report a single centre experience of patients with PACS and suspected autonomic dysfunction. Methods: Forty-two patients evaluated in the Outpatient Cardiology Department with suspected PASC were included in the study. Patients complained of compromised exercise performance persisting >3 months after recovery from COVID-19 infection, compared to the pre-COVID-19 period. The patients were evaluated with 12-lead electrocardiogram, echocardiography, 24-hour ECG ambulatory monitoring and either exercise stress test or a 6-minute walk test. Results: All 42 patients demonstrated an exaggerated chronotropic response, defined as the inappropriate increase in heart rate before the 6th minute of exercise >100% of the age-predicted maximal heart rate value with reproduction of clinical symptoms. In addition, 24-hour ambulatory electrocardiography revealed an increased mean heart rate of 92 beats/minute and decreased mean standard deviation of sequential 5-minute N-N interval (SDNN) of 74.4 ms. Pharmaceutical treatment with b-blockers, ivabradine or both was administrated in 29 (69%) resulting in symptomatic improvement in 82.8% of those under treatment. However, residual symptoms persisted in 69% of patients after 3 months. Conclusions: In patients with “Post-acute COVID-19” syndrome, we found an excessive chronotropic response to exercise suggesting autonomic dysfunction as the underlying mechanism of symptoms. Treatment with beta blockers or ivabradine resulted in clinical improvement but a substantial proportion of patients remained symptomatic.
文摘The objective of this work was to elaborate ceramic water filters from Kaolinite (Cameroon) clay for the elimination of suspended particles from domestic drinking water. In Sub-Sahara Africa and in Cameroon in particular health issues have been linked to the consummation of domestic tap water of high turbidity values both in the rural and urban areas. In order to remedy these problems, ceramic water pot filters have been elaborated in a pilot scale unit with aim of putting in place a unit production. The chemical composition, the thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC) analyses of the raw materials (clay and rice husks) was determined. The crystal phases and scanning electron microscope of Wack clay was also determined. The ceramic pot filter membranes were fabricated from the formulations 70/20/10 of clay/porogen/chamotte respectively with the particle size of the raw material less than or equal to 500 μm. The formulated ceramic pot filters were then sintered at 900˚C in a furnace. These ceramic pot filters were characterized by determining their porosity, withdrawal percentages, water permeability, mechanical and chemical resistance. The study of the efficiency consisted in evaluating the retention rate and permeate flux with respect to time (days) with synthetic water suspensions of turbidity 100 NTU and particle size of 2 μm. The ceramic pot filters were made aiming at studying the efficiency after physical defouling of filters. Physical defouling consisted in brushing the inner surface of the ceramic pot filters with water and drying them at ambient temperature after being used for 11 days and reusing them under the same initial conditions. The produced ceramic pot filter had a volume of 4 L, an average porosity of 36.15%, shrinkage in mass or withdrawal percentage of 18.23%, a water permeability of 59.6 × 10<sup>3</sup> L∙h<sup>−2</sup>∙m<sup>−</sup><sup>2</sup>, mechanical resistance of 6.8 MPa and corrosion resistance of 1.6% in acidic medium and 0.8% in alkaline medium. The evaluation of the retention efficiency reveals that the retention rate of 99.9% was obtained from the 9<sup>th</sup> day of filtration reducing the turbidity value from 100 NTU to less than 0.1 NTU. From the filtration test carried out during the 11 consecutive days, the flow rate varied between 1.46 L∙h<sup>−1</sup> to 2.63 L∙h<sup>−1</sup>. Similar results of retention and flow rate were obtained after physical defouling of the ceramic pot filter membranes and re-using for 11 consecutive days, showing the efficiency of the ceramic pot filter membranes in eliminating suspended particles from drinking water. Cost evaluation for the production unit reveals a total cost of production for 50 ceramic pot filters of 1593.6 USD consisting of fixed assets and variable assets. An estimated selling price of 3.3 USD was obtained which is affordable for both the urban and rural population in Cameroon and in sub-Saharan Africa.
文摘The objective of this work was to elaborate ceramic water filters from Kaolinite (Cameroon) clay for the elimination of suspended particles from domestic drinking water. In Sub-Sahara Africa and in Cameroon in particular health issues have been linked to the consummation of domestic tap water of high turbidity values both in the rural and urban areas. In order to remedy these problems, ceramic water pot filters have been elaborated in a pilot scale unit with aim of putting in place a unit production. The chemical composition, the thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC) analyses of the raw materials (clay and rice husks) was determined. The crystal phases and scanning electron microscope of Wack clay was also determined. The ceramic pot filter membranes were fabricated from the formulations 70/20/10 of clay/porogen/chamotte respectively with the particle size of the raw material less than or equal to 500 μm. The formulated ceramic pot filters were then sintered at 900˚C in a furnace. These ceramic pot filters were characterized by determining their porosity, withdrawal percentages, water permeability, mechanical and chemical resistance. The study of the efficiency consisted in evaluating the retention rate and permeate flux with respect to time (days) with synthetic water suspensions of turbidity 100 NTU and particle size of 2 μm. The ceramic pot filters were made aiming at studying the efficiency after physical defouling of filters. Physical defouling consisted in brushing the inner surface of the ceramic pot filters with water and drying them at ambient temperature after being used for 11 days and reusing them under the same initial conditions. The produced ceramic pot filter had a volume of 4 L, an average porosity of 36.15%, shrinkage in mass or withdrawal percentage of 18.23%, a water permeability of 59.6 × 10<sup>3</sup> L∙h<sup>−2</sup>∙m<sup>−</sup><sup>2</sup>, mechanical resistance of 6.8 MPa and corrosion resistance of 1.6% in acidic medium and 0.8% in alkaline medium. The evaluation of the retention efficiency reveals that the retention rate of 99.9% was obtained from the 9<sup>th</sup> day of filtration reducing the turbidity value from 100 NTU to less than 0.1 NTU. From the filtration test carried out during the 11 consecutive days, the flow rate varied between 1.46 L∙h<sup>−1</sup> to 2.63 L∙h<sup>−1</sup>. Similar results of retention and flow rate were obtained after physical defouling of the ceramic pot filter membranes and re-using for 11 consecutive days, showing the efficiency of the ceramic pot filter membranes in eliminating suspended particles from drinking water. Cost evaluation for the production unit reveals a total cost of production for 50 ceramic pot filters of 1593.6 USD consisting of fixed assets and variable assets. An estimated selling price of 3.3 USD was obtained which is affordable for both the urban and rural population in Cameroon and in sub-Saharan Africa.
文摘The People’s Army of Vietnam (PAVN) decided to use conventional warfare to unify Vietnam. The United States military operation against the Ho Chi Minh Trail traffic began in 1959. The (PAVN) connected a series of old trails leading from North Vietnam to South Vietnam via Laos and Cambodia. In the 1960s the network of trails was expanded. Trail traffic was interdicted by repeated CIA (Air America) and US Air Force (Operation Ranch Hand) tactical herbicide spraying and bombing missions. During the late 1960s, the Khmer Rouge Army slowly grew in eastern Cambodia during a time when America was spraying and bombing the Ho Chi Minh Trail. On February 22, 1969, the PAVN launched a new offensive against American forces in South Vietnam from their sanctuaries in Cambodia. President Richard Nixon and Dr. Henry Kissinger, decided to spray and bomb Cambodia, a neutral country, to eliminate the PAVN sanctuary base camps. The damage and loss of life as a result of U.S. air campaign resulted in the insurgency (Khmer Rouge) being able to recruit civilian members. This created a dual effect of strengthening the popularity of the Khmer Rouge, led by Pol Pot, enabling him to overthrow the Khmer Republic in 1975. The primary objectives of this study are to determine the political impacts and consequences of: 1) the 1959 United States secret war on the Ho Chi Minh Trail in Cambodia and 2) the 1969 President Nixon’s decision to destroy the PAVN bases hidden in the Cambodian jungles. These United States secret wars in Cambodia had long-lasting effects on Cambodian political, social, and economic stability.