ACUPUNCTURE EFFECT ON GENE EXPRESSION OF c-fosmRNA, ppENKmRNA, iNOSmRNA AND iNOS ACTIVITY IN THE MOUSE SPINAL CORD

To examine the relationship between expression of the c fosmRNA, ppENKmRNA, iNOSmRNA, iNOS activity and acupuncture analgesia, the acupuncture effect on expression of them in the mouse spinal cord was studied. 20 BALB/C mice were randomly divided into 2 groups: a. the acupuncture group treated with 5 Hz electroacupuncture(EA); b. the control group treated with no EA. The gene expression of c fos, ppENK and iNOS in the spinal cord was detected by using in situ hybridization and RNA dot blot techniques. The iNOS activity was detected by using histochemistry and protein dot blot. The dot blot signals were scanned for statistical analysis. The results showed that the hybridization signals appeared bluish violet color. The c fos signals were localized in the laminae I-II and V-VII, the ppEMK weak signals localized in the superficial lamina and the iNOS signals localized in the cytoplasm of astrocytes of the mouse spinal cord. The pain threshold and all of the dot blot signals were enhanced in the acupuncture group when compared to that in the control group, P<0.05. In the acupuncture group, the intensity of expression of c fosmRNA, ppENKmRNA, iNOSmRNA and iNOS activity was positively correlated with analgesia effect respectively, P<0.05-0.01. The enhanced expression of iNOSmRNA and iNOS suggests that the spinal glia may play a more active role in synaptic function to cause analgesia induced by acupuncture stimulation. Besides, there was a positive correlation in alteration between spinal c fosmRNA and ppENK or c fosmRNA and iNOSmRNA, suggesting that c fos may be involved in ppENKmRNA transcription and c fos and iNOS may have common transactivators.

针刺对小鼠巨噬细胞基因表达的效应(英文)

目的 为了探讨针刺镇痛效应与细胞免疫的关系 ,研究了针刺对小鼠巨噬细胞中 c- fos m RNA,pp ENKm RNA,i NOSm RNA及 i NOS活性的效应。 方法 2 0只 BAL B/ c小鼠被随机分成 2组 :a.用 5 Hz电针处理的针刺组 ;b.未用电针处理的对照组。针刺或牵拉前后用钾离子渗透法检测痛阈。实验采用原位杂交 ,NADPH-NBT组织化学 ,RNA斑点印迹及蛋白质斑点印迹技术。应用 TL C扫描仪扫描斑点印迹信号并作统计学处理。 结果 杂交信号和 i NOS活性呈现蓝紫色 ,信号均定位于巨噬细胞的胞质。与对照组相比 ,针刺组的所有印迹信号均增强 ,P<0 .0 1。针刺组中 c- fos m RNA,pp ENKm RNA,i NOSm RNA的表达及 i NOS的活性与提高的痛阈呈正相关。此外 ,c- fos m RNA与 pp ENKm RNA之间的变动呈正相关。 结论 针刺可上调小鼠巨噬细胞中 c- fos,pp ENK,i NOS的基因表达 ,且提示 c- fos可能参与 pp ENK基因表达 ,c- fosm RNA与 pp ENKm RNA可能同时表达。