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Parental Knowledge of Pre-Hospital Management of Avulsed Permanent Tooth in Children at Kath
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作者 Ama Agyeibea Amuasi Samuel K. Addo Solomon Obiri-Yeboah 《Open Journal of Stomatology》 2024年第2期87-102,共16页
BACKGROUND: Permanent tooth avulsion is one of the severe forms of dental traumatic injuries. The immediate action taken at the site of the accident is crucial to the prognosis of the tooth. Replantation is considered... BACKGROUND: Permanent tooth avulsion is one of the severe forms of dental traumatic injuries. The immediate action taken at the site of the accident is crucial to the prognosis of the tooth. Replantation is considered as the treatment of choice. OBJECTIVE: The study was undertaken to assess the knowledge of parents who accompany their children to the pediatric dental clinic, KATH on the pre-hospital management of avulsed permanent tooth in children. METHODS: A researcher administered structured questionnaire was used to collect data on the knowledge of pre-hospital management of avulsed permanent tooth from 83 parents who accompanied their wards to the pediatric dental clinic at KATH. RESULTS: A total of 83 parents were involved in the study. 30 (36%) were males while 53 (64%) were females. The majority of the parents (57%) were either university trained or had attended college of education. Only 32 parents (39%) were aware of the possibility of replantation. Majority of the parents chose non-physiologic media as the transport media of choice and only 10% would attempt self-replantation before seeking professional help. 76 parents (92%) had no previous education on pre-hospital management of avulsed tooth. CONCLUSION: The results obtained from this study indicate that parental knowledge on pre-hospital management of avulsed permanent tooth is low hence the need for massive public educational campaigns. 展开更多
关键词 Parental Knowledge Tooth Avulsion pre-hospital Management Permanent Tooth
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Correlation Study of Neurotransmitter and Immune Levels in Pre-Hospital Emergency Nurses with Post-Traumatic Stress Disorder
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作者 Yanling Zhou Min Guo +1 位作者 Xiangling Jiang Long Li 《Journal of Behavioral and Brain Science》 2024年第1期12-22,共11页
Objective: To investigate the occurrence of PTSD in pre-hospital emergency nurses and its related factors, and to compare the differences of neurotransmitter and immune-related factors between pre-hospital emergency n... Objective: To investigate the occurrence of PTSD in pre-hospital emergency nurses and its related factors, and to compare the differences of neurotransmitter and immune-related factors between pre-hospital emergency nurses who experienced traumatic events and those who did not develop PTSD and healthy people. How: Post-traumatic Stress Disorder Self-Rating Scale (PCL-C) tests were performed on pre-hospital emergency nurses in PTSD group, non-PTSD group and healthy control group, and the plasma monoamine neurotransmitters and serum cytokines were determined by double-antibody sandwich ABC-ELISA assay using enzyme-linked adsorption kit provided by Shanghai Xitang Biotechnology Co., Ltd. Results: 1) There were statistically significant differences in PCL-C scores between PTSD group, non-PTSD group and healthy group (p α between PTSD group, non-PTSD group and healthy group (p Conclusion: Pre-hospital emergency nurses should have early psychological intervention and guidance to reduce the occurrence of PTSD in emergency and emergency nurses. 展开更多
关键词 pre-hospital First Aid NURSE Post-Traumatic Stress Disorder NEUROIMMUNOLOGY
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Pre-Hospital Management of Craniocerebral Trauma in South Saharan Africa: Cotonou Experience 被引量:4
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作者 Abd El Kader Moumouni Essossinam Kpelao +5 位作者 Holden Olatoundji Fadigba Agbeko Achille Doleagbenou Ibrahima Berete Dabou Abiba Tamou Tabe Emile Mensah Martin Chobli 《Open Journal of Modern Neurosurgery》 2019年第3期221-226,共6页
The management of craniocerebral trauma begins at the site of the accident. This is one of the diseases for which pre-hospital care is well codified. The objective of this study is to report the experience of the Emer... The management of craniocerebral trauma begins at the site of the accident. This is one of the diseases for which pre-hospital care is well codified. The objective of this study is to report the experience of the Emergency Aid Medical Service (EMAS) of BENIN in the pre-hospital management of craniocerebral trauma. 146 head injuries were cared for. 116 were male. The mean age was 32 years with extremes ranging from 2 to 77 years. Etiology in 68% of patients was a public road accident. And the request for the use of the EMAS was made by highway users for 41% of the injuries. About 5.5% were severe cranio-brain injuries, more than 75% of which had died in intensive care. In 77% of the EMAS interventions, there was an intensive care physician in the team. 34 wounded had been transported in a vacuum mattress. The rigid cervical collar was used in 8 major traumas. All the wounded were transported by ambulance. The duration of hospitalization varied from a few hours in the emergency department to 90 days in the hospital ward. 9 patients died. One of them, who died in an ambulance, was a woman. The prehospital medical care of head trauma in BENIN is still progressing. 展开更多
关键词 Emergency CRANIOCEREBRAL AFRICA South-Sahara pre hospital MANAGEMENT
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Current pre-hospital traumatic brain injury management in China 被引量:18
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作者 Kou Kou iang-yu Hou +1 位作者 Jian-dong Sun Kevin Chu 《World Journal of Emergency Medicine》 CAS 2014年第4期245-254,共10页
BACKGROUND: Traumatic brain injury(TBI) is associated with most trauma-related deaths. Secondary brain injury is the leading cause of in-hospital deaths after traumatic brain injury. By early prevention and slowing of... BACKGROUND: Traumatic brain injury(TBI) is associated with most trauma-related deaths. Secondary brain injury is the leading cause of in-hospital deaths after traumatic brain injury. By early prevention and slowing of the initial pathophysiological mechanism of secondary brain injury, prehospital service can signifi cantly reduce case-fatality rates of TBI. In China, the incidence of TBI is increasing and the proportion of severe TBI is much higher than that in other countries. The objective of this paper is to review the pre-hospital management of TBI in China.DATA SOURCES: A literature search was conducted in January 2014 using the China National Knowledge Infrastructure(CNKI). Articles on the assessment and treatment of TBI in pre-hospital settings practiced by Chinese doctors were identified. The information on the assessment and treatment of hypoxemia, hypotension, and brain herniation was extracted from the identifi ed articles.RESULTS: Of the 471 articles identified, 65 met the selection criteria. The existing literature indicated that current practices of pre-hospital TBI management in China were sub-optimal and varied considerably across different regions.CONCLUSION: Since pre-hospital care is the weakest part of Chinese emergency care, appropriate training programs on pre-hospital TBI management are urgently needed in China. 展开更多
关键词 TRAUMATIC brain INJURY pre-hospital China EMERGENCY MEDICINE
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French pre-hospital trauma triage criteria:Does the “prehospital resuscitation” criterion provide additional benefit in triage? 被引量:1
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作者 Emmanuel Hornez Olga Maurin +3 位作者 Aurélie Mayet Tristan Monchal Federico Gonzalez Delphine Kerebel 《World Journal of Critical Care Medicine》 2014年第3期68-73,共6页
AIM:To evaluate the performance of the specific French Vittel "Pre-Hospital(PH) resuscitation" criteria in selecting polytrauma patients during the pre-hospital stage and its potential to increase the positi... AIM:To evaluate the performance of the specific French Vittel "Pre-Hospital(PH) resuscitation" criteria in selecting polytrauma patients during the pre-hospital stage and its potential to increase the positive predictive value(PPV) of pre-hospital trauma triage.METHODS: This was a monocentric prospective cohort study of injured adults transported by emergency medi-cal service to a trauma center. Patients who met any of the field trauma triage criteria were considered "triage positive". Hospital data was statistically linked to prehospital records. The primary outcome of defining a "major trauma patient" was Injury Severity Score(ISS) > 16. RESULTS: There were a total of 200 injured patients evaluated over a 2 years period who met at least 1 triage criterion. The number of false positives was 64 patients(ISS < 16). The PPV was 68%. The sensitivity and the negative predictive value could not be evaluated in this study since it only included patients with positive Vittel criteria. The criterion of "PH resuscitation" was present for 64 patients(32%),but 10 of them had an ISS < 16. This was statistically significant in correlation with the severity of the trauma in univariate analysis(OR = 7.2; P = 0.005; 95%CI: 1.6-31.6). However,despite this correlation the overall PPV was not significantly increased by the use of the criterion "PH resuscitation"(68% vs 67.8%).CONCLUSION: The criterion of "pre-hospital resuscitation" was statistically significant with the severity of the trauma,but did not increase the PPV. The use of "prehospital resuscitation" criterion could be re-considered if these results are confirmed by larger studies. 展开更多
关键词 pre-hospital TRIAGE Vittel CRITERIA Injury Severity Score TRAUMA
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Comparison Study of Art Therapy and Play Therapy in Reducing Anxiety on Pre-School Children Who Experience Hospitalization 被引量:1
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作者 Sri Ramdaniati Susy Hermaningsih Muryati 《Open Journal of Nursing》 2016年第1期46-52,共7页
Pain and hospitalization caused a crisis in the child’s life. At the pre-school children, the stress of the disease makes children become less able to cope with separation. As a result, many children show anxious beh... Pain and hospitalization caused a crisis in the child’s life. At the pre-school children, the stress of the disease makes children become less able to cope with separation. As a result, many children show anxious behavior though more vague than toddler age children. Various ways have been conducted by nurses to treat anxiety in children, but the most effective types of the intervention have not be found. Types of intervention that can be done are art therapy and play therapy. This study aims to compare the effects of art therapy and play therapy in reducing anxiety on children who experience hospitalization. A quasi-experimental research design with pre-post test two group design is used. The sample was 23 children pre-school age for art therapy group and 25 children for play therapy with sampling techniques performed purposive sampling. Intervention of art therapy and play therapy each performed for 3 days with duration about 30 minutes. Anxiety levels before and after the intervention were measured by using the facial affective scale. The results show that there is a difference in anxiety between before and after the action in the art therapy group (p = 0.00) and that there are differences in anxiety before and after the action in the play therapy group (0.00). But statistical tests using Man Whitney U indicate that there is no difference in anxiety levels in pre-school children who do art therapy and play therapy (p = 0.26). So, it is concluded that the art therapy and the play therapy can reduce the level of anxiety on pre-school school children that experience hospitalization. Based on these conclusions, it is recommended that the art therapy and the play therapy can be used by nurses and the choice of the intervention should be submitted to the child. 展开更多
关键词 Art Therapy Play Therapy pre-School Children hospitalIZATION
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Evaluation of Management of Pre-Eclamptic Cases Admitted to Elshatby Maternity University Hospital
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作者 Nermeen Mohamed Hefila Tamer Mamdouh Abd Eldayem Hisham Adel El Fazari 《Open Journal of Obstetrics and Gynecology》 2021年第6期689-700,共12页
Hypertensive diseases of pregnancy, mainly PET, consist of several dise</span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style=&qu... Hypertensive diseases of pregnancy, mainly PET, consist of several dise</span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">a</span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">ses which are associated with increased morbidity and mortality rates both maternal and fetal wise. Its incidence among pregnant females is about 3</span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">% </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">-</span></span></span><span><span><span style="font-family:""> </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">10%.</span></span></span><span><span><span style="font-family:""> </span></span></span><span><span><span style="font-family:""><span style="font-family:Verdana;">This study included 500 pregnant PET women, and they categorized to mild and severe forms, after exclusion of cases with essential hypertension, renal disease and SLE. All pregnant ladies underwent history taking, abdominal examination (general and local), obstetric ultrasound, Doppler ultrasound, laboratory investigations;treatment received (antihypertensive medications, magnesium sulphate (MgSO</span><sub><span style="font-family:Verdana;">4</span></sub><span style="font-family:Verdana;">) and steroids) and finally follow up till delivery. Regarding complications, PET can result in serious fetal/neonatal or maternal complications.</span></span></span></span><span><span><span style="font-family:""> </span></span></span><span><span><span style="font-family:""><span style="font-family:Verdana;">By the beginning of this study we started with 380 mild cases and 120 severe cases, but the end of the study 50 cases of mild PET were lost during follow up and 70 cases of mild PET converted into severe form. The aim of this study is to evaluate the management protocols of preeclamptic cases admired to Elshatby maternity hospital to minimize maternal and fetal morbidity and mortality. RESULTS: The results showed that the incidence of PET is significant in both young and old age, higher incidence of IUGR in sever PET, the use of corticosteroids and MgSO</span><sub><span style="font-family:Verdana;">4</span></sub><span style="font-family:Verdana;"> was significantly higher in sever cases. CONCLUSION: it’s important to diagnose PET by repeated blood pressure measurement. The use of corticosteroids and MgSO</span><sub><span style="font-family:Verdana;">4</span></sub><span style="font-family:Verdana;"> is improving maternal and fetal outcomes significantly. The incidence of C.S. is higher towards prematurity in sever cases. 展开更多
关键词 pre-ECLAMPSIA preeclampsia Management Elshatby hospital
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Clinical Research of Pre-Hospital Emergency Care, Nursing, Infection Prevention and Control for Senile Osteoporotic Vertebral Compression Fracture during Epidemic Period
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作者 Ying Zhang Xinming Yang +1 位作者 Yanlin Yin Peinan Zhang 《Open Journal of Preventive Medicine》 CAS 2022年第12期249-257,共9页
Background: Novel coronavirus pneumonia (NCP) and osteoporotic vertebral compression fractures (OVCF) are the high incidences of diseases in the elderly. During the epidemic period, if not treated in time, the complic... Background: Novel coronavirus pneumonia (NCP) and osteoporotic vertebral compression fractures (OVCF) are the high incidences of diseases in the elderly. During the epidemic period, if not treated in time, the complications are high and the mortality is high. If we do not pay attention to infection prevention and control in pre-hospital emergency care, it will lead to the first time infection of medical staff and in-hospital cross infection in emergency outpatient receiving area. The correct consideration of both and the establishment of perfect pre-hospital emergency treatment and infection prevention and control synchronous strategy is an important premise to ensure the stable, orderly and safe medical treatment. Objective: To explore the effect of synchronous implementation of pre-hospital emergency care, nursing and infection pre-vention and control for senile OVCF during the epidemic. In order to improve the efficiency of pre-hospital emergency care and prevent the spread of infection. Method: A total of 92 elderly patients with OVCF who received pre-hospital treatment in 18 hospitals in Zhangjiakou City during the epidemic prevention and control period from January 2020 to November 2022 and met the inclusion criteria were selected as research objects, including 24 males and 68 females, aged 65 - 82 (74.2 ± 2.2) years. All patients were associated with concomitant injuries and underlying diseases. All patients in this group underwent predictive pre-hospital rescue and infection prevention and control procedures. Results: All the 92 elderly patients with OVCF received timely pre-hospital treatment during the epidemic period, and no aggravation occurred of the 92 patients, 35 were in the high risk area, 10 were in the medium risk area, and 47 were in the low risk area. Exclude OVCF for NCP Patients were treated according to the conventional diagnosis and treatment principles. Suspected and confirmed cases are transferred to designated surgical hospitals for treatment. All patients were followed up 3 months, 6 months and 12 months after treatment. There was no death rate, high satisfaction of pre-hospital first aid, high diagnostic accuracy, and good curative effect. None of the rescue personnel had any infection rate, and no hospital infection transmission and nosocomial cross infection occurred. Conclusion: It is the first step to safely treat patients and prevent cross infection to establish a perfect synchronous strategy of pre-hospital first aid and infection prevention and control. 展开更多
关键词 Novel Coronavirus Pneumonia Osteoporotic Vertebral Compression Fracture pre-hospital First Aid On-Site Treatment Epidemic Risk Assessment Screening Process Infection prevention and Control Synchronization
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基于动物模型的院前临时VV-ECMO治疗高原重度肺冲击伤的研究
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作者 伍正彬 邵世锋 +4 位作者 张良潮 段朝霞 王耀丽 梁宗安 王建民 《局解手术学杂志》 2024年第5期373-378,共6页
目的通过在高海拔地区进行自由场化爆实验,建立山羊重度肺冲击伤动物模型,探讨临时静脉-静脉体外膜肺氧合(VV-ECMO)技术在现场早期救治中的可行性。方法选择成年山羊16只,按照随机数字表法分为对照组和救治组,每组8只,在海拔4600 m高原... 目的通过在高海拔地区进行自由场化爆实验,建立山羊重度肺冲击伤动物模型,探讨临时静脉-静脉体外膜肺氧合(VV-ECMO)技术在现场早期救治中的可行性。方法选择成年山羊16只,按照随机数字表法分为对照组和救治组,每组8只,在海拔4600 m高原环境下建立重度肺冲击伤模型,随后对照组山羊给予呼吸机支持,救治组山羊给予临时VV-ECMO救治。记录伤后15 min山羊存活情况,比较2组山羊伤前和伤后1 h、2 h、3 h生命体征[体温、呼吸频率、心率、平均动脉压(MAP)]及动脉血气分析指标[pH、动脉血氧分压(PO_(2))、动脉血二氧化碳分压(PCO_(2))、动脉血氧饱和度(SaO_(2))、乳酸(LAC)、血钙离子(Ca^(2+))、红细胞压积(HCT)、血红蛋白(Hb)]。对所有死亡和救治结束后处死的山羊进行尸检,采用器官损伤分级(OIS)评估肺损伤严重程度,依据简明损伤评分(AIS)进行肺损伤评分。计算肺组织湿重与干重比值(W/D)和肺系数。结果爆炸后15 min内对照组山羊死亡4只,存活4只;救治组山羊死亡5只,存活3只。2组山羊受伤前后体温、呼吸频率、心率、MAP组间比较,差异均无统计学意义(P>0.05)。救治组山羊伤后1 h、2 h、3 h PO_(2)、SaO_(2)均优于对照组(P<0.05),伤后2 h Ca^(2+)显著高于对照组(P<0.05);2组山羊伤后不同时间点pH、PCO_(2)、LAC、HCT、Hb比较差异均无统计学意义(P>0.05)。2组山羊伤后OIS、AIS、肺系数比较,差异无统计学意义(P>0.05),但对照组肺组织W/D较救治组低(P<0.05)。结论在高海拔地区,我们首次建立了一种新型、可行性强、救治效果稳定的临时VV-ECMO动物救治策略,可以为高原地区重度肺冲击伤现场早期的VV-ECMO救治提供动物实验依据。 展开更多
关键词 爆震伤 山羊 体外膜肺氧合 高原 院前急救 肺冲击伤
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Perception and attitude of theatre staff to preoperative HIV testing at the University of Port Harcourt Teaching Hospital
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作者 Odigie JO Siminialayi IM 《Asian Pacific Journal of Tropical Medicine》 SCIE CAS 2010年第2期145-147,共3页
Objective:To elucidate the awareness and approach to pre-operative human immunodeficiency virus(HIV) testing and emphasis on the attendant components of HIV testing.Methods: The study was conducted at the theatres of ... Objective:To elucidate the awareness and approach to pre-operative human immunodeficiency virus(HIV) testing and emphasis on the attendant components of HIV testing.Methods: The study was conducted at the theatres of the University of Port Harcourt Teaching Hospital among 123 nursing,anaesthetic,and operating staff.A descriptive cross sectional design was adopted with stratified sampling.The study instrument was a structured,self administered pro forma.Results:All respondents were all aware of HIV infection and all had transmission through infected blood and tissues(100.0%).88.6%had infection through needle stick injuries, 62.6%through vertical transmission,and 98.4%through blood transfusion.Sixty three percent of respondents correctly knew what preoperative testing was,while 58.5%were aware of the preoperative testing policy of the hospital.All respondents favoured the policy of preoperative testing.Attitudinal values to seropositive patients were not very different as 72.4%of respondents claimed they treated every patient as high risk,and all respondents used personal protective equipment.Conclusions:Routine HIV testing now represents a conventional means providing patients with knowledge of their HIV status.Such testing should be accompanied by informed consent,counselling,confidentiality,protection,and access to treatment. 展开更多
关键词 Human IMMUNODEFICIENCY virus pre OPERATIVE HIV testing University of PORT Harcourt Teaching hospital
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农村院前急救的基层延伸:建设“村民-村医”自救互救环节
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作者 黄思语 胡玉瑶 +4 位作者 刘雨婷 黄欣扬 李悦芃 焦语凝 焦光源 《中国急救复苏与灾害医学杂志》 2024年第2期174-176,182,共4页
目的 在现有农村“院前急救-院内急救”急救体系下,提出新增建设“村民-村医”自救互救环节,延伸农村院前急救链,主要以村民与村医的角度深入探究建设困境,并多维度提出完善策略,为提升农村院前急救水平提供新思路。方法 走访我国5省市... 目的 在现有农村“院前急救-院内急救”急救体系下,提出新增建设“村民-村医”自救互救环节,延伸农村院前急救链,主要以村民与村医的角度深入探究建设困境,并多维度提出完善策略,为提升农村院前急救水平提供新思路。方法 走访我国5省市6地区,并对13名基层急救相关人员(村民、村医、村干部)开展质性访谈,深入调研建设“村民-村医”自救互救环节的困境和具体建议。结果 建设“村民-村医”自救互救环节存在困境:村民的急救素养有待提高,表现在急救意识、急救技能掌握等方面;乡村基层医疗机构的服务水平仍需加强,表现在急救能力、药物与设施配备等方面。结论 从人力资源、物质基础、信息宣传和法律保障4个角度入手,可为有效建设“村民-村医”自救互救环节提供助力,进一步完善农村院前急救体系。 展开更多
关键词 院前急救 农村 自救互救 村民 村医
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Epidemio-Clinical, Therapeutic and Evolutive Aspects of Pulmonary Embolism in Young Subject in the Cardiology Department in Point “G” Hospital University Center Bamako
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作者 M. Sako S. Sidibé +12 位作者 M. Konaté B. Sonfo N. Diallo M. Diakité C. Thiam A. Keita I. Sangaré H. O. Ba Y. Camara L. Bouaré Y. R. Koumaré S. Coulibaly I. Minta 《Open Journal of Epidemiology》 2020年第4期393-398,共6页
<strong>Objective:</strong> The purpose of this work was to determine the epidemiological, clinical and evolutionary aspects of the pulmonary embolism of the young person in the cardiology department of th... <strong>Objective:</strong> The purpose of this work was to determine the epidemiological, clinical and evolutionary aspects of the pulmonary embolism of the young person in the cardiology department of the University Hospital Center (CHU) Point G in Bamako-Mali. <strong>Methodology:</strong> This was an analytical study from January 01, 2018 to December 31, 2018 in the CHU Point G cardiology department, including all patients hospitalized during this period. <strong>Results:</strong> Of 1379 hospitalized patients, 19 patients were concerned by pulmonary embolism of the young person. The prevalence of pulmonary embolism of the young person was 1.37%. The most affected age group was 21 - 30 (47.4%) of patients. The predominance was female (89.47%) with a gender ratio of 0.11 in favour of women. The average age in the series was 29.79 years with extremes of 16 years and 40 years. Factors predisposing to pulmonary embolism were dominated by peri partum, cardiomyopathy and obesity with 47.3%, 31.57% and 21.1%, respectively. The dominant signs were chest pain and dyspnea in 94.7% and 89.5% of cases respectively. Pulmonary embolism was unlikely in 60.52% according to the Geneva and Wells score simplified. At the thoracic angioscanner, the embolism was bilateral in 52.6% of cases and distal in 36.8% of patients;in 10 patients who performed cardiac ultrasound, pulmonary arterial hypertension (70%), dilation of the right ventricle (20%) and left ventricular dilation (40%). Hyper leucocytosis (47.4%), anemia and low prothrombin rate (TP) (22.2%) were the most found biological abnormalities. More than 2/3 (68.5%) our patients had an intermediate mortality risk according to the PESI (Pulmonary Embolism Severity Index) score. The average hospital stay was 10 days. Hospital mortality was 10.5%. <strong>Conclusion:</strong> The pulmonary embolism of the young person is a frequent, serious and multifactorial pathology and the female sex is most affected especially during peri partum periods, hence the need for preventive measures. Clinical signs are not specific and based on the assessment of clinical probability. Pulmonary angioscanner remains the confirmation review in our context. 展开更多
关键词 Pulmonary Embolism Young Subject 15 - 40 Years CARDIOLOGY Point G hospital
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HBV PreS2+S/IFN-α融合基因真核表达载体的构建及其表达 被引量:3
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作者 陈红梅 白雪帆 +3 位作者 潘蕾 李光玉 韦三华 黄长形 《中国病毒学》 CSCD 2002年第4期312-314,共3页
构建含HBVPrdS2 +S和IFN α融合基因的真核表达载体pcDNA3.1.S2S/IFN α并在真核细胞中进行表达。应用重叠延伸剪切技术 (splicingbyoverlappingextension ,简称SOE)经两次PCR获得嵌合基因片段S2S/IFN α ,回收后直接克隆到 pcDNA3.1V5 ... 构建含HBVPrdS2 +S和IFN α融合基因的真核表达载体pcDNA3.1.S2S/IFN α并在真核细胞中进行表达。应用重叠延伸剪切技术 (splicingbyoverlappingextension ,简称SOE)经两次PCR获得嵌合基因片段S2S/IFN α ,回收后直接克隆到 pcDNA3.1V5 /HisTOPOTA克隆载体 ,得到真核重组载体pcDNA3.1.S2S/IFN α。然后用脂质体法转染VeroE6细胞。对重组载体进行了限制性酶切及PCR鉴定 ,证明连接正确 ;经间接免疫荧光检测证实该重组载体能在真核细胞中表达插入的外源性基因编码的融合蛋白。真核表达载体pcDNA3.1.S2S/IFN α的成功构建及在VeroE6细胞中的有效表达 。 展开更多
关键词 HBV preS2+S/INF-α融合基因 真核表达载体 乙型肝炎病毒 Α-干扰素 基因表达
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G-FAST评分对院前急救卒中前循环大血管闭塞的诊断价值研究 被引量:1
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作者 穆彩琴 李书宁 +3 位作者 马青峰 王田慧 赵伟豪 李斗 《北京医学》 CAS 2023年第5期389-392,共4页
目的探讨凝视-面-臂-言语-时间(gaze-face-arm-speech-time,G-FAST)评分对院前急救卒中前循环大血管闭塞(large vessel occlusion in the anterior circulation,aLVO)的诊断价值。方法选取2019年7月至2020年12月北京急救中心直属5个分... 目的探讨凝视-面-臂-言语-时间(gaze-face-arm-speech-time,G-FAST)评分对院前急救卒中前循环大血管闭塞(large vessel occlusion in the anterior circulation,aLVO)的诊断价值。方法选取2019年7月至2020年12月北京急救中心直属5个分中心送至宣武医院,且有完整院前G-FAST评分和入院诊断信息的卒中患者,根据缺血性卒中患者是否发生LVO分为LVO和非LVO组,采用ROC曲线分析G-FAST评分对院前卒中急救aLVO的诊断价值。结果纳入患者352例,其中急性缺血性卒中占比69.0%(243/352)。进行大血管评估的急性缺血性卒中患者149例,占急性缺血性卒中的61.3%(149/243);发生aLVO患者61例,占大血管病变评估的40.9%(61/149)、占急性缺血性卒中的25.1%(61/243)。149例大血管评估的急性缺血性卒中患者中,男100例,女49例;年龄18~93岁,平均70.5岁。与非aLVO组相比,aLVO组女性较多,G-FAST评分较高,差异均有统计学意义(P<0.05)。G-FAST≥3分患者的aLVO发生率显著高于G-FAST≤2分者(68.9%比31.1%),差异有统计学意义(P<0.05)。G-FAST评分诊断院前急救卒中aLVO的ROC曲线的AUC为0.675(95%CI:0.589~0.761,P=0.000),G-FAST的cut-off值为2.5分时,灵敏度为72.10%,特异度为58.00%。结论G-FAST评分在院前急救卒中可准确识别急性缺血性患者aLVO,早诊断aLVO将利于急性缺血性患者尽早送至高级别卒中中心。 展开更多
关键词 凝视---言语-时间评分 急性缺血性卒中 院前急救 前循环大血管闭塞
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Preβ-1高密度脂蛋白与心血管疾病关系的研究进展 被引量:1
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作者 李凤芹 聂宏刚 刘友斌 《中国心血管病研究》 CAS 2019年第1期5-8,12,共5页
动脉粥样硬化是心血管疾病重要的病理生理基础,延缓和防治动脉粥样硬化对于减少和降低心血管疾病的发病率及病死率具有重要意义。高密度脂蛋白(high density lipoprotein,HDL)通过参与介导胆固醇逆向转运(reverse cholesterol transport... 动脉粥样硬化是心血管疾病重要的病理生理基础,延缓和防治动脉粥样硬化对于减少和降低心血管疾病的发病率及病死率具有重要意义。高密度脂蛋白(high density lipoprotein,HDL)通过参与介导胆固醇逆向转运(reverse cholesterol transport,RCT)在抗动脉粥样硬化的形成和进展中发挥了重要作用。Preβ-1高密度脂蛋白(prebeta-1 high density lipoprotein,Preβ-1HDL)作为HDL的一种亚类,是外周细胞移出胆固醇的最初接受体,直接参与了RCT的起始步骤,并在随后的胆固醇酯化及转运中起着重要作用。本文就Preβ-1HDL的结构、代谢及其与心血管疾病的关系作一简要综述。 展开更多
关键词 preβ-1高密度脂蛋白 胆固醇逆转运 动脉粥样硬化 心血管疾病
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知识-信念-行为管理模式用于医院新职工岗前培训的探索
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作者 周磊 王召 《安徽医专学报》 2023年第6期1-3,7,共4页
目的:探索知识一信念一行为管理模式用于医院新职工岗前培训的方法与效果。方法:整群抽取在某医院入职的新职工67名作为研究对象,分为研究组(34名)与对照组(33名)。对照组采用传统的培训方法,研究组采用知识一信念-行为管理模式培训方法... 目的:探索知识一信念一行为管理模式用于医院新职工岗前培训的方法与效果。方法:整群抽取在某医院入职的新职工67名作为研究对象,分为研究组(34名)与对照组(33名)。对照组采用传统的培训方法,研究组采用知识一信念-行为管理模式培训方法,两组培训时间为3个月,记录与判定两组的培训效果。结果:培训3个月后,研究组新职工的卫生法规、“三基”培训、应急管理、专项培训等理论考核成绩与对照组相比都显著提高(P<0.05)。研究组新职工的培训水平、培训内容、培训方法、培训效果等总体质量评分与对照组相比都显著提高(P<0.05);研究组新职工的培训满意度显著高于对照组(P<0.05);研究组新职工的医疗差错事件发生率显著低于对照组(P<0.05)。结论:知识一信念一行为管理模式用于医院新职工岗前培训不仅能提高职工的理论考核成绩,还可提高其综合素质、能力,有利于提高职工的满意度,降低医疗差错事件发生率。 展开更多
关键词 知识-信念-行为管理模式 医院 新职工 岗前培训
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创伤院前救护能力现状及延时现场救护知识和能力准备-多中心横断面调研 被引量:4
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作者 张翔 袁乙铜 +4 位作者 何静 谢菲 陈蓓婧 胡光云 舒勤 《创伤外科杂志》 2023年第3期216-222,共7页
目的了解我国军、地医院创伤院前救护能力现状及延时现场救护(prolonged field care,PFC)认知及能力准备情况,为相关培训提供参考。方法2021年2月—6月,采用分层抽样和便利抽样相结合的方法,选取东北、西南、中部、东南以及珠三角地区24... 目的了解我国军、地医院创伤院前救护能力现状及延时现场救护(prolonged field care,PFC)认知及能力准备情况,为相关培训提供参考。方法2021年2月—6月,采用分层抽样和便利抽样相结合的方法,选取东北、西南、中部、东南以及珠三角地区242名军队及地方医护人员,采用自行设计的《中国创伤院前急救及延时现场救护(PFC)认知情况调查问卷》,借助于问卷星平台进行多中心横断面调查。结果(1)部队三甲医院年均院前救护任务显著多于地方二甲医院(P<0.05),道路交通伤在所有伤型中占比最高;(2)“途中持续监护和复苏预案”是制定最多的院前救护预案,“转运时间过长”是反映最多的问题;(3)PFC知晓率仅为35.1%;(4)“现场处理能力”和“复杂伤情评估”被分别认为是军、地医院创伤院前急救培训中最急需和最缺少的能力。结论当前我国军、地医院救护人员对创伤院前急救措施的掌握情况不容乐观,对各类预案了解不足,PFC概念知晓度普遍较低,缺乏复杂伤情评估和决策能力,亟待完善培训策略,提升整体创伤院前救护能力。 展开更多
关键词 创伤 院前救护 延时现场救护 能力 急救培训
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院前-院内无缝急救模式对急性脑梗死患者阿替普酶静脉溶栓治疗的疗效影响 被引量:4
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作者 冯奕敢 陈迪 +1 位作者 李建 宁玉梅 《中国处方药》 2023年第10期8-10,共3页
目的 探讨院前-院内无缝急救模式对急性脑梗死患者阿替普酶静脉溶栓治疗的疗效影响。方法 选取2020年10月~2022年10月某院急诊收治的78例行阿替普酶静脉溶栓治疗的急性脑梗死患者为研究对象,依据急救模式不同分为两组,试验组(120接诊院... 目的 探讨院前-院内无缝急救模式对急性脑梗死患者阿替普酶静脉溶栓治疗的疗效影响。方法 选取2020年10月~2022年10月某院急诊收治的78例行阿替普酶静脉溶栓治疗的急性脑梗死患者为研究对象,依据急救模式不同分为两组,试验组(120接诊院前-院内无缝急救,37例)和对照组(自行入院常规院内急救,41例)。统计两组急诊救治成功率;比较观察两组入院至接诊、CT检查、实验室检查、静脉溶栓四项急诊救治工作所用时间;溶栓后24 h,以美国国立卫生研究院卒中量表(NIHSS)评估两组神经功能缺损改善情况;随访3个月,以改良Rankin量表(mRS)和Bathel指数(BI)评估两组神经功能恢复情况及日常生活能力,评价两组预后。结果 两组均在时间窗内完成了阿替普酶静脉溶栓治疗并生存,急诊救治成功率100%。试验组患者入院至接诊、CT检查、实验室检查、静脉溶栓四项急诊救治工作所用时间均短于对照组,差异有统计学意义(P <0.05)。试验组溶栓后NIHSS评分低于对照组,治疗后随访BI评分高于对照组,m RS≥2分患者占比低于对照组,差异有统计学意义(P <0.05)。结论 基于院前-院内无缝急救模式对急性脑梗死患者进行阿替普酶静脉溶栓治疗,可以有效缩短院内救治时间,提高溶栓治疗效果,在改善患者预后方面更具优势。 展开更多
关键词 急性脑梗死 阿替普酶 静脉溶栓 院前-院内无缝急救模式 疗效
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Feasibility study of emergency intervention for vascular injury outside the hospital
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作者 Ming Liang Jing-Jing Rong +5 位作者 Jing-Yang Sun Xiao-Zeng Wang Fei Li Geng Wang Yan-Chun Liang Ya-Ling Han 《Military Medical Research》 SCIE CAS 2017年第2期80-85,共6页
Background: Minimally invasive surgery in the field of traumatic vascular injury diagnosis and treatment has achieved good results. This study was designed to determine whether pre-hospital emergency intervention is f... Background: Minimally invasive surgery in the field of traumatic vascular injury diagnosis and treatment has achieved good results. This study was designed to determine whether pre-hospital emergency intervention is feasible for vascular injury in a field intervention cabin under the condition of war or a disaster site.Methods: Different types of animal experiments of vascular injury intervention were performed in a field intervention cabin. Treatment capacity was evaluated by data collection, including duration of surgery, clinical evaluation, image clarity, and equipment handling. Environmental adaptability and mobility were evaluated by maneuverability and long-distance mobility.Results: A total of 56 surgeries(7 types) were performed in the field intervention cabin. Digital subtraction angiography(DSA) had good imaging performance. A total of 4800 km of long-distance mobility was performed, and all the equipment operated normally without any equipment failure. We participated in the medical service maneuver twice. The cabin unfolded and worked properly. There was no equipment damage during the medical service maneuver.Conclusion: Use of a field intervention cabin under the conditions of war or disaster is feasible for pre-hospital emergency intervention of vascular injury. 展开更多
关键词 Vascular injury pre-hospital emergency INTERVENTION CABIN
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2018-2022年苏州市院前急救卒中患者特征分析 被引量:1
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作者 王冬阳 秦义人 +2 位作者 戴斌 刘永平 林岐 《江苏卫生事业管理》 2023年第11期1526-1530,共5页
目的:分析苏州市院前急救卒中患者分布情况,调整资源布局,整体提升卒中救治能力。方法:以智慧急救平台为基础,从苏州市急救中心提取2018-2022年院前急救卒中患者病历资料,进行回顾性分析。结果:院前急救卒中患者男女比为1.67∶1,平均年... 目的:分析苏州市院前急救卒中患者分布情况,调整资源布局,整体提升卒中救治能力。方法:以智慧急救平台为基础,从苏州市急救中心提取2018-2022年院前急救卒中患者病历资料,进行回顾性分析。结果:院前急救卒中患者男女比为1.67∶1,平均年龄为68.79±15.47岁;平均呼救-到院时间为38.34±17.28分钟;一年中院前急救卒中病例主要集中于冬季(10-12月),一天中的高峰时段为7-19时;主要发生区域在姑苏区、吴江区和吴中区,卒中病例既往病史分布最高的三项为高血压(58.06%)、脑部疾病(22.72%)和糖尿病(19.81%);FAST症状分布中肢体无力(68.82%)、言语不清(57.49%)、面瘫/口角歪斜(34.92%)在年龄分布方面存在显著差异。结论:院前急救卒中病例在人群、时间和区域的分布上存在一定的规律,相关部门可优化区域急救资源配置,提高院前医务人员的卒中救治水平,并针对高危因素的老龄人口进行卒中科普教育,以全面提升卒中病例的早期发现和早期救治能力。 展开更多
关键词 院前急救 卒中 特征分布
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