Mimotope peptide modified pompon mum-like magnetic microparticles for precise recognition,capture and biotransformation analysis of rituximab in biological fluids

Due to low immobilized ligand density,limited binding capacity,and severe interference from serum proteins,developing ideal peptide-based biomaterials for precise recognition and in vivo analysis of biopharmaceuticals remains a huge challenge.In this study,mimotope peptide modified pompon mum-like biomimetic magnetic microparticles(MMPs,3.8μm)that mimic the specific functionalities of CD20 on malignant B cells were developed for the first time.Benefit from the numerous ligand binding sites(Ni^(2+))on the pompon mum-like MMPs,these novel materials achieved≥10 times higher peptide ligand densities(>2300 mg/g)and antibody binding capacities(1380 mg/g)compared to previous reported biomaterials.Leveraging the high specificity of the mimotope peptide,rituximab can be precisely recognized and enriched from cell culture media or serum samples.We also established an LC-MS/MS method using the MMPs for tracking rituximab biotransformation in patient serum.Intriguingly,deamidation of Asn55 and Asn33,as well as oxidation of Met81 and Met34 were observed at the key complementarity determining regions of rituximab,which could potentially influence antibody function and require careful monitoring.Overall,these versatile biomimetic MMPs demonstrate superior recognition and enrichment capabilities for target antibodies,offering interesting possibilities for biotransformation analysis of biopharmaceuticals in patient serum.

Realize Facilitated Targeted Poverty Elimination with Targeted Poverty——Alleviation: A New Anti-Poverty Approach for Rural China

Since the Reform and Opening-up, remarkable achievements in poverty alleviation have been made in China. China's success in mass poverty reduction can be attributed to its rapid economic growth, large-scale regional poverty alleviation and development, sound social security policies, widereaching farmer-benefiting policies and the equal land allotment system. However, with income inequality on the rise, poverty reduction effect made by the economic growth is declining: More targeted poverty alleviation policies are needed by China. Targeted poverty alleviation aims to improve the effect and efficiency of poverty alleviation through precise identification of poverty-stricken populations and comprehensive supportive measures. To tackle a variety of challenges such as the poor cultivation among povertystricken populations, the complex causes for poverty, and inflexible capital management, China should improve its poverty alleviation mechanism by innovating poverty identification methods, support approaches, capital management and performance assessment.