The profile of polypeptides separated by SDS-PAGE from seed of major crop species such as pea(Pisum sativum) is complex,resulting from cleavage (processing) of precursors expressed from multiple copies of genes encodi...The profile of polypeptides separated by SDS-PAGE from seed of major crop species such as pea(Pisum sativum) is complex,resulting from cleavage (processing) of precursors expressed from multiple copies of genes encoding vicilin and legumin,the major storage globulins.Translation in vitro of mRNAs hybridselected from mid-maturation pea seed RNAs by defined vicilin and legumin cDNA clones provided precursor molecules that were cleaved in vitro by a cell-free protease extract obtained from similar stage seed;the derived polypeptides were of comparable sizes to those observed in vivo.The feasibility of transcribing mRNA in vitro from a cDNA clone and cleavage in vitro of the derived translation products was established for a legumin clone,providing a method for determining polypeptide products of an expressed sequence.This approach will also be useful for characterising cleavage site requirements since modifications an readily be introduced at the DNA level.展开更多
The rockburst of the poal pillar under a thick hard roof stratum is modelled as the instability failure problem of coal pillars under strata subject to elastic support. The instability mechanism of rockburst is studie...The rockburst of the poal pillar under a thick hard roof stratum is modelled as the instability failure problem of coal pillars under strata subject to elastic support. The instability mechanism of rockburst is studied by applying cusp catastrophic theory. The effects of the stiffness ratio of the system and loads imposed on the system on the rockburst are explicated.The factors affecting rockbursts are discussed. Based on them, the evolution process, the forewarning regularity arid forewarning sings of rockbursts are studied. It is indicated that the subsidence velocity of roof stratum, which increases quickly and tends to infinity, is the forewarning measurable signs of the rockbursts of coal pillar.展开更多
This paper deals with the fuzzy information processing method in the research of the relationship between two variables or among multi-variables, with the undetermined or fuzzy features, different from that in the tr...This paper deals with the fuzzy information processing method in the research of the relationship between two variables or among multi-variables, with the undetermined or fuzzy features, different from that in the traditional statistical method. The reliability and effectiveness of the method have been tested and confirmed in the numerical simulation for a set of man-made data of precursory anomalous parameters and earthquake elements. Finally the relation between the actual monthly frequency of small earthquakes occurring in Huoshan, Anhui Province, China and the magnitude of future stronger earthquakes, as two variables, has been analyzed by the method. It seems to the authors that more reasonable and perfect results could be given with a quantitative analysis of accession degree in fuzzy mathematics by using this method than that of traditional statistical correlation analysis.展开更多
In order to quantitatively describe the geoelectric precursor anomaly in the short-impending process of earthquakes, a new geoelectric precursor index — (monthly) relative change rate of ground resistivity, R ρ...In order to quantitatively describe the geoelectric precursor anomaly in the short-impending process of earthquakes, a new geoelectric precursor index — (monthly) relative change rate of ground resistivity, R ρ (t) , is designed. Using this index and choosing the internationally accepted ground resistivity data before the Tangshan M =7.8 earthquake of July 28, 1976, the features of dynamic evolution pattern of R ρ(t) are studied. The results show that: ① about 10～9 months before earthquake, the ground resistivity in a certain range around the epicentral region begins to display the anomaly of accelerating descent, and the rate of descent is higher in the epicentral region than in surrounding areas; ② with the shortening of countdown before earthquake, the R ρ(t) value in epicentral region increases gradually (ground resistivity value decreases at an increasing rate); ③ the R ρ(t) value has the epicentral area as a center and its contour lines propagate towards surrounding areas with the shortening of countdown before earthquake; ④after the R ρ(t) value in epicentral region has descended at increasing rate to reach an extremity [ R ρ(t) = (7.0], it turns to descend at decreasing rate (2～3 months) and earthquake occurs when it accelerates again. Meanwhile, earthquake occurs when the contour lines of R ρ(t) stop propagating towards surrounding areas and turn to shrink back (2～3 months later). Its physical process can be explained by the″ swollen hypothesis″ of Prof. Fu and the theory of ″Slip-weakening and rockmass instability″ of Mei, Niu, et al ..展开更多
The calculation method of the location of the singular generalized current source according to synchronous measurements of the magnetic field in the remote points is proposed. It is designed for the continuous monitor...The calculation method of the location of the singular generalized current source according to synchronous measurements of the magnetic field in the remote points is proposed. It is designed for the continuous monitoring of changes of the degree of electromagnetic heterogeneity of the geophysical environment. Regular fluctuations of the apparent distance to the effective single source were recorded according to the magnetic data of the observatories in Japan 2009-2011. They are connected with the rhythm of the geodynamic processes in the vicinity of the station Kakioka. The most significant decrease in the apparent distance was registered in connection with the seismic activation of the region, including the catastrophic earthquake of March 11, 2011 with a magnitude of M = 9.1. The anomalous behavior of the apparent parameters of the generalized source was displayed not less than two weeks before the earthquake. Characteristic changes of the curves were registered in the period of three months.展开更多
Using the view point of nonlinear science and the method of selecting numerical features of pattern recognition for reference, the physical and numerical features of precursory ground tilt data are synthetically emplo...Using the view point of nonlinear science and the method of selecting numerical features of pattern recognition for reference, the physical and numerical features of precursory ground tilt data are synthetically employed. The dynamic changes of data series are described with the numerical features in multi dimensional space and their distributive relations instead of an unique factor. The relationship between the ground tilt data and earthquake is examined through recognition and classification.展开更多
The mechanism of precursor ionization ahead of strong shock waves has been studied in a low density shock tube. The experimental results are illustrated with Arrhenius plots with kink points dividing them into two par...The mechanism of precursor ionization ahead of strong shock waves has been studied in a low density shock tube. The experimental results are illustrated with Arrhenius plots with kink points dividing them into two parts with apparent activation energy ratio 1:2, namely with the values 7.7 eV and 15.3 eV, and varying with first and third power of the density respectively. A model is proposed to interpret the facts where the process taking place in the precursor region is a two step photo ionization accompanied with the drift flow effect of the gas relative to the shock wave or the ionization recombination effect according to whether the shock speed and initial density are low enough. The product of the A-A collision excitation cross section coefficient S* multiplied by the radiation cross section Q of Argon S×Q=1×10^(-36)(cm^4eV^(-1)) and the three body recombination coefficient of Argon at room temperature k_(ra)=1×10^(-24)(cm^(-6)s^(-1)).展开更多
文摘The profile of polypeptides separated by SDS-PAGE from seed of major crop species such as pea(Pisum sativum) is complex,resulting from cleavage (processing) of precursors expressed from multiple copies of genes encoding vicilin and legumin,the major storage globulins.Translation in vitro of mRNAs hybridselected from mid-maturation pea seed RNAs by defined vicilin and legumin cDNA clones provided precursor molecules that were cleaved in vitro by a cell-free protease extract obtained from similar stage seed;the derived polypeptides were of comparable sizes to those observed in vivo.The feasibility of transcribing mRNA in vitro from a cDNA clone and cleavage in vitro of the derived translation products was established for a legumin clone,providing a method for determining polypeptide products of an expressed sequence.This approach will also be useful for characterising cleavage site requirements since modifications an readily be introduced at the DNA level.
文摘The rockburst of the poal pillar under a thick hard roof stratum is modelled as the instability failure problem of coal pillars under strata subject to elastic support. The instability mechanism of rockburst is studied by applying cusp catastrophic theory. The effects of the stiffness ratio of the system and loads imposed on the system on the rockburst are explicated.The factors affecting rockbursts are discussed. Based on them, the evolution process, the forewarning regularity arid forewarning sings of rockbursts are studied. It is indicated that the subsidence velocity of roof stratum, which increases quickly and tends to infinity, is the forewarning measurable signs of the rockbursts of coal pillar.
文摘This paper deals with the fuzzy information processing method in the research of the relationship between two variables or among multi-variables, with the undetermined or fuzzy features, different from that in the traditional statistical method. The reliability and effectiveness of the method have been tested and confirmed in the numerical simulation for a set of man-made data of precursory anomalous parameters and earthquake elements. Finally the relation between the actual monthly frequency of small earthquakes occurring in Huoshan, Anhui Province, China and the magnitude of future stronger earthquakes, as two variables, has been analyzed by the method. It seems to the authors that more reasonable and perfect results could be given with a quantitative analysis of accession degree in fuzzy mathematics by using this method than that of traditional statistical correlation analysis.
文摘In order to quantitatively describe the geoelectric precursor anomaly in the short-impending process of earthquakes, a new geoelectric precursor index — (monthly) relative change rate of ground resistivity, R ρ (t) , is designed. Using this index and choosing the internationally accepted ground resistivity data before the Tangshan M =7.8 earthquake of July 28, 1976, the features of dynamic evolution pattern of R ρ(t) are studied. The results show that: ① about 10～9 months before earthquake, the ground resistivity in a certain range around the epicentral region begins to display the anomaly of accelerating descent, and the rate of descent is higher in the epicentral region than in surrounding areas; ② with the shortening of countdown before earthquake, the R ρ(t) value in epicentral region increases gradually (ground resistivity value decreases at an increasing rate); ③ the R ρ(t) value has the epicentral area as a center and its contour lines propagate towards surrounding areas with the shortening of countdown before earthquake; ④after the R ρ(t) value in epicentral region has descended at increasing rate to reach an extremity [ R ρ(t) = (7.0], it turns to descend at decreasing rate (2～3 months) and earthquake occurs when it accelerates again. Meanwhile, earthquake occurs when the contour lines of R ρ(t) stop propagating towards surrounding areas and turn to shrink back (2～3 months later). Its physical process can be explained by the″ swollen hypothesis″ of Prof. Fu and the theory of ″Slip-weakening and rockmass instability″ of Mei, Niu, et al ..
文摘The calculation method of the location of the singular generalized current source according to synchronous measurements of the magnetic field in the remote points is proposed. It is designed for the continuous monitoring of changes of the degree of electromagnetic heterogeneity of the geophysical environment. Regular fluctuations of the apparent distance to the effective single source were recorded according to the magnetic data of the observatories in Japan 2009-2011. They are connected with the rhythm of the geodynamic processes in the vicinity of the station Kakioka. The most significant decrease in the apparent distance was registered in connection with the seismic activation of the region, including the catastrophic earthquake of March 11, 2011 with a magnitude of M = 9.1. The anomalous behavior of the apparent parameters of the generalized source was displayed not less than two weeks before the earthquake. Characteristic changes of the curves were registered in the period of three months.
文摘Using the view point of nonlinear science and the method of selecting numerical features of pattern recognition for reference, the physical and numerical features of precursory ground tilt data are synthetically employed. The dynamic changes of data series are described with the numerical features in multi dimensional space and their distributive relations instead of an unique factor. The relationship between the ground tilt data and earthquake is examined through recognition and classification.
基金The project supported by the National Natural Science Foundation of China
文摘The mechanism of precursor ionization ahead of strong shock waves has been studied in a low density shock tube. The experimental results are illustrated with Arrhenius plots with kink points dividing them into two parts with apparent activation energy ratio 1:2, namely with the values 7.7 eV and 15.3 eV, and varying with first and third power of the density respectively. A model is proposed to interpret the facts where the process taking place in the precursor region is a two step photo ionization accompanied with the drift flow effect of the gas relative to the shock wave or the ionization recombination effect according to whether the shock speed and initial density are low enough. The product of the A-A collision excitation cross section coefficient S* multiplied by the radiation cross section Q of Argon S×Q=1×10^(-36)(cm^4eV^(-1)) and the three body recombination coefficient of Argon at room temperature k_(ra)=1×10^(-24)(cm^(-6)s^(-1)).
