The soil nearby a gas station was collected, from which we isolated a series of strains by means of enrichment and screening technigues. The most effective strain for the degradation of toluene, i.e., JYZ0315 was scre...The soil nearby a gas station was collected, from which we isolated a series of strains by means of enrichment and screening technigues. The most effective strain for the degradation of toluene, i.e., JYZ0315 was screened by the further mutation of the above strains. The degradation capacity experiment was carried out in simulated groundwater. Under the optimal conditions, the degradation rate could reach 93.5%. During the extend application experiments with a percolation tank, the degradation of toluene reached 87.3% in 11 d and 95.2% in 15 d, which was close to that of the microenvironment, and this proves that JYZ0315 is one of the predominant strains to degrade toluene in groundwater.展开更多
[Objective] The study aimed at selecting the predominant strains being able to degrade kerosene and studying its best growth conditions. [Method] Choosing kerosene as the only carbon source, we selected and separated ...[Objective] The study aimed at selecting the predominant strains being able to degrade kerosene and studying its best growth conditions. [Method] Choosing kerosene as the only carbon source, we selected and separated the predominant strains being able to degrade kerosene from the contaminated soil near petrochemical plants, and then optimized the grow conditions of the bacteria. [Result] The best conditions for the bacteria growth were determined as follows, that is, temperature was 30 ℃, pH=7, salinity was 2.5%, and the rotational speed of the thermostatic shake was 190 r/min. Under the optimal conditions, the degradation rate of kerosene by the bacteria cultured for three days reached 42.6%. [Conclusion] The research could provide scientific references for the restoration of polluted soil by kerosene.展开更多
基金Supported by Doctoral Funds(No.19990 1870 3) and the National Natural Science Foundation of China(No.4 9872 0 83)and the Key L aboratory of the Ministry of L and and ResourcesP.R.China
文摘The soil nearby a gas station was collected, from which we isolated a series of strains by means of enrichment and screening technigues. The most effective strain for the degradation of toluene, i.e., JYZ0315 was screened by the further mutation of the above strains. The degradation capacity experiment was carried out in simulated groundwater. Under the optimal conditions, the degradation rate could reach 93.5%. During the extend application experiments with a percolation tank, the degradation of toluene reached 87.3% in 11 d and 95.2% in 15 d, which was close to that of the microenvironment, and this proves that JYZ0315 is one of the predominant strains to degrade toluene in groundwater.
基金Supported by National Key Technology R&D Program of China (2010BAD03B01)
文摘[Objective] The study aimed at selecting the predominant strains being able to degrade kerosene and studying its best growth conditions. [Method] Choosing kerosene as the only carbon source, we selected and separated the predominant strains being able to degrade kerosene from the contaminated soil near petrochemical plants, and then optimized the grow conditions of the bacteria. [Result] The best conditions for the bacteria growth were determined as follows, that is, temperature was 30 ℃, pH=7, salinity was 2.5%, and the rotational speed of the thermostatic shake was 190 r/min. Under the optimal conditions, the degradation rate of kerosene by the bacteria cultured for three days reached 42.6%. [Conclusion] The research could provide scientific references for the restoration of polluted soil by kerosene.
