Detection of α_(2u)-globulin in rat pup preputial gland by MALDI-TOF mass spectrometry

The α_ 2u-globulin,a soluble protein identified in the urine and preputial gland of adult male rat is reported to be pheromone carrier.The pup preputial gland plays a significant role in chemical communication for mother-young interaction;however,the presence of a pheromone-carrying protein in the pup preputial gland has not been confirmed.Therefore,the present study was carried out to identify the α_ 2u-globulin in the pup preputial gland by Matrix Assisted Laser Desorption Ionization Time of Flight mass spectrometry(MALDI-TOF).The preputial glands of prepubertal rats were subjected to one-dimensional SDS-PAGE.In-gel trypsin digestion of a 18 kDa band was carried out and analyzed by MALDI-TOF.The results of a MASCOT search showed the presence of α_ 2u-globulin in the 18 kDa band.In contrast to the report of the synthesis of this protein only in adult rats,the identification of this protein in pup preputial gland is significant.The results suggest that synthesis of α_ 2u-globulin in the rat preputial gland starts in the prepubertal stage itself.In prepubertal rats,the preputial gland is a source of pheromone for performing anogenital licking behaviour by the mother rat.Since α_ 2u-globulin belongs to the lipocalin(ligand carrier)family,it might carry the volatile for processing pheromonal communication in mother-pup bonding in rat.

Role of preputial washing in reducing microbial load and improving bovine semen quality

Quality semen production remains the main focus and objective of semen processing laboratories throughout the world. Bacterial and other microbial contaminants affect the semen quality and hence the fertility, and also lead to reproductive disorders as well as lower conception rates and increased embryonic mortality, abortion and other complications in females. Microbial contamination affects the semen adversely, by exerting direct spermicidal effect, formation of reactive oxygen species, toxin production, adherence with spermatozoa, deriving nutrients and oxygen from the medium and thus competing with spermatozoa for the factors of growth and normal functioning. Despite hygienic measures, several ubiquitous and opportunistic microbes find their ways into semen during collection, processing, and storage of semen, and survive even during freezing. Stringent sanitary precautions are therefore required at every step of collecting semen and its processing. Preputial cavity is considered as main source of semen contaminating microorganisms. Flushing the preputial cavity with normal saline or any suitable liquid combination with antimicrobial activity, prior to semen collection reduces the microbial load and thereby improves the semen quality.

One-stage urethroplasty with circumferential vascular pedicle preputial island flap for perineal hypospadias

Objective To report the treatment of perineal hypospadias with one - stage urethroplaty with circumferential vascular pedicle preputial island flap. Methods A circumferential incision was made proximal to the cprona and the urethral plate to correct chordee. A U - shaped skin incision was then made surrounding the meatus。

Double dartos flap layer in tubularized incised plate urethroplasty to prevent urethrocutaneous fistula in uncircumcised patients with distal hypospadias

Urethrocutaneous fistula may complicate hypospadias repair.We noticed that double-layered preputial dartos flaps added to tubularized incised plate urethroplasty can reduce the risk of urethrocutaneous fistula.The aim of this study was to compare the outcomes of tubularized incised plate urethroplasty with double-layered preputial dartos flaps to with single-layered local fascial flaps in preventing urethrocutaneous fistula.A retrospective cohort study was conducted between January 2017 and December 2020 at Jordan University Hospital(Amman,Jordan).Boys who were aged between 6 months and 5 years,diagnosed with distal hypospadias,and not circumcised were included.The primary outcome was the occurrence of urethrocutaneous fistula in patients who underwent tubularized incised plate urethroplasty with a double-layered fascial flap.The results showed a total of 163 boys with distal hypospadias;among them,116 patients underwent tubularized incised plate urethroplasty with a single-layered fascial flap,and 47 underwent tubularized incised plate urethroplasty with a double-layered fascial flap.The development of urethrocutaneous fistula was higher in the group receiving tubularized incised plate urethroplasty with a single-layered fascial flap than in the group receiving tubularized incised plate urethroplasty with a double-layered fascial flap after 1 month,6 months,and 12 months(6.9%vs 0,10.3%vs 0,and 5.2%vs 0,respectively),and the difference after 6 months was statistically significant(P=0.02).

Flaps for bulbar urethral ischemic necrosis in pelvic fracture urethral injury

Bulbar urethral ischemic necrosis(BUIN)is an iatrogenic entity resulting from repeated attempts at performing anastomotic urethroplasty for pelvic fracture urethral injuries.Etiologically speaking,BUIN is related to a compromised blood supply of the bulbar urethra,which normally relies on anterograde supply from bulbar arteries and retrograde supply from recurrent branches of dorsal penile arteries,through the glans.At each transection of the bulbar urethra,both the anterograde and retrograde supplies are compromised,increasing the risk of BUIN.Even though this term is widely used among reconstructive urologists,BUIN is orphan of an accepted scientific definition.We aim to report our personal perspective on BUIN,to identify factors associated with its occurrence,and to describe the management options in these patients.