Collective cell migration is a coordinated movement of multi-cell systems essential for various processes throughout life.The collective motions often occur under spatial restrictions,hallmarked by the collective rota...Collective cell migration is a coordinated movement of multi-cell systems essential for various processes throughout life.The collective motions often occur under spatial restrictions,hallmarked by the collective rotation of epithelial cells confined in circular substrates.Here,we aim to explore how geometric shapes of confinement regulate this collective cell movement.We develop quantitative methods for cell velocity orientation analysis,and find that boundary cells exhibit stronger tangential ordering migration than inner cells in circular pattern.Furthermore,decreased tangential ordering movement capability of collective cells in triangular and square patterns are observed,due to the disturbance of cell motion at unsmooth corners of these patterns.On the other hand,the collective cell rotation is slightly affected by a convex defect of the circular pattern,while almost hindered with a concave defect,also resulting from different smoothness features of their boundaries.Numerical simulations employing cell Potts model well reproduce and extend experimental observations.Together,our results highlight the importance of boundary smoothness in the regulation of collective cell tangential ordering migration.展开更多
Composite nonlinear feedback （CNF） control techniquefor tracking control problems is extended to the output regulationproblem of singular linear systems with input saturation. A statefeedback CNF control law and an ...Composite nonlinear feedback （CNF） control techniquefor tracking control problems is extended to the output regulationproblem of singular linear systems with input saturation. A statefeedback CNF control law and an output feedback CNF controllaw are constructed respectively for the output regulation problemof singular linear systems with input saturation. It is shown thatthe output regulation problem by CNF control is solvable underthe same solvability conditions of the output regulation problemby linear control. However, with the virtue of the CNF control, thetransient performance of the closed-loop system can be improvedby carefully designing the linear part and the nonlinear part of theCNF control law. The design procedure and the improvement ofthe transient performance of the closed-loop system are illustratedwith a numerical simulation.展开更多
基金supported by the National Natural Science Foundation of China(Nos.12174208 and 32227802)National Key Research and Development Program of China(No.2022YFC3400600)+2 种基金Guangdong Major Project of Basic and Applied Basic Research(No.2020B0301030009)Fundamental Research Funds for the Central Universities(Nos.2122021337 and 2122021405)the 111 Project(No.B23045).
文摘Collective cell migration is a coordinated movement of multi-cell systems essential for various processes throughout life.The collective motions often occur under spatial restrictions,hallmarked by the collective rotation of epithelial cells confined in circular substrates.Here,we aim to explore how geometric shapes of confinement regulate this collective cell movement.We develop quantitative methods for cell velocity orientation analysis,and find that boundary cells exhibit stronger tangential ordering migration than inner cells in circular pattern.Furthermore,decreased tangential ordering movement capability of collective cells in triangular and square patterns are observed,due to the disturbance of cell motion at unsmooth corners of these patterns.On the other hand,the collective cell rotation is slightly affected by a convex defect of the circular pattern,while almost hindered with a concave defect,also resulting from different smoothness features of their boundaries.Numerical simulations employing cell Potts model well reproduce and extend experimental observations.Together,our results highlight the importance of boundary smoothness in the regulation of collective cell tangential ordering migration.
基金supported by the National Natural Science Foundation of China(61374035)
文摘Composite nonlinear feedback （CNF） control techniquefor tracking control problems is extended to the output regulationproblem of singular linear systems with input saturation. A statefeedback CNF control law and an output feedback CNF controllaw are constructed respectively for the output regulation problemof singular linear systems with input saturation. It is shown thatthe output regulation problem by CNF control is solvable underthe same solvability conditions of the output regulation problemby linear control. However, with the virtue of the CNF control, thetransient performance of the closed-loop system can be improvedby carefully designing the linear part and the nonlinear part of theCNF control law. The design procedure and the improvement ofthe transient performance of the closed-loop system are illustratedwith a numerical simulation.
