Objective： To screen and analyze key express sequence tags （ESTs） which were differentially displayed in every period of SD rats＇ primary hepatic carcinoma and reveal the molecular mechanism of carcinogenesis. Met...Objective： To screen and analyze key express sequence tags （ESTs） which were differentially displayed in every period of SD rats＇ primary hepatic carcinoma and reveal the molecular mechanism of carcinogenesis. Methods： Using diethylnitrosamine （DENA） as a cancerigenic agent, animal models with different phases of primary hepatic cancer were constructed in SD rats. Rats were respectively sacrificed at d 14, d 28, d 56, d 77, d 105 and d 112 after the rats received DENA by gavage, then the livers were harvested. One part of the livers was classified according to their pathological changes, while the other was reserved for molecular mechanism studies on hepatocarcinogenesis. The differentially expressed genes were isolated from both normal and morbid tissues by mRNA differential display technique （DDRT-PCR）. After the fragments were sequenced, bioinformatics were .used to analyze the results. Results： Twelve differentially expressed cDNA fragments were obtained. Nine fragments had the homology with known cDNA clones, especially EST-7 was similar to BN/SsNHsdMCW mitochondrion gene and the identity was 100% which suggested EST-7 may be the part of BN/SsNHsdMCW mitochondrion gene. In contrast, other three fragments （EST-1, EST-3 and EST-5） had extremely low identity to any genes registered in GENBANK databases. Conclusions： BN/SsNHsdMCW mitochondrion gene was expressed in different periods of hepatocarcinogenesis. Moreover, EST-I, EST-3 and EST-5 were suggested to contribute to the development of rat hepatocarcinogenesis, and thus may be candidates of new targets of oncogenes or cancer suppressor genes.展开更多
Objective To explore the significance of union examination of blood serum liver cancer tracers in the early diagnosis of liver cancer. Methods We observed and compared the level of blood serum liver cancer tracers arm...Objective To explore the significance of union examination of blood serum liver cancer tracers in the early diagnosis of liver cancer. Methods We observed and compared the level of blood serum liver cancer tracers armor embryo protein (AFP),crag algae glycosidase (AFU),armor embryo protein heteroplasmon (AFPL3) and γ-Gu Anxian transferase (γ-GT) in early time for primary liver cancer patients and hepatitis liver cirrhosis patients and those chronic hepatitis B patients who had liver cancer family history. Results Finally among the 30 patients in the early liver cancer group,23 were positive with AFP,20 with AFU,15 with AFPL3 and 21 with γ-GT. Five were found positive with blood serum AFP,AFPL3,AFU and γ-GT at the same time; 5 with AFP,AFPL3 and γ-GT; 5 with AFP,AFU and AFPL3; 7 with AFP,AFU and γ-GT. By contrast,in the control group,among the 30 hepatitis liver cirrhosis patients and those chronic hepatitis B patients with liver cancer family history,11 were found positive with AFP,3 with AFPL3,12 with AFU and 14 with γ-GT. None of the patients were found positive with union examination of AFP,AFPL3,AFU and γ-GT in the blood serum at the same time. Conclusion The union examination of AFP,AFU,AFPL3 and γ-GT is significant to the early diagnosis of primary liver cancer.展开更多
目的分析在诊断原发性肝癌患者时采取血清α-L-岩藻糖苷酶(α-l-fucosidase,AFU)、甲胎蛋白(α-fetoprotein,AFP)、异常凝血酶原Ⅱ(Protein Induced by Vitamin K Absence or Antagonist-Ⅱ,PIVKA-Ⅱ)检测的价值。方法选取2022年8月—2...目的分析在诊断原发性肝癌患者时采取血清α-L-岩藻糖苷酶(α-l-fucosidase,AFU)、甲胎蛋白(α-fetoprotein,AFP)、异常凝血酶原Ⅱ(Protein Induced by Vitamin K Absence or Antagonist-Ⅱ,PIVKA-Ⅱ)检测的价值。方法选取2022年8月—2023年8月邳州市中医院收治的83例原发性肝癌患者以及肝硬化患者作为研究对象,依据疾病类型不同,分为良性组(肝硬化患者41例)、观察组(原发性肝癌患者42例),另选择83例健康人群作为对照组。检测3组血清AFU、AFP、PIVKA-Ⅱ指标,分析上述指标水平,同时通过ROC曲线分析单项以及联合检测诊断原发性肝癌的价值。结果两组患者AFU、AFP、PIVKA-Ⅱ指标均显著高于对照组,且观察组AFU、AFP、PIVKA-Ⅱ指标水平高于良性组,差异有统计学意义(P均<0.05)。联合检测时,AUC提升至0.912,灵敏度提升至89.63%,特异度提升至99.10%,高于单独检测。结论在诊断原发性肝癌患者中,采用联合AFU、AFP、PIVKA-Ⅱ检测方式,有较为理想的诊断价值,能够为完善治疗方案奠定基础,可以作为诊断原发性肝癌的有效方法之一。展开更多
Objective: To study the correlation of serum GP73, SOD and GPC3 contents with cell proliferation and angiogenesis in liver cancer lesion. Methods: Patients who were diagnosed with primary liver cancer in Jianghan Oilf...Objective: To study the correlation of serum GP73, SOD and GPC3 contents with cell proliferation and angiogenesis in liver cancer lesion. Methods: Patients who were diagnosed with primary liver cancer in Jianghan Oilfield General Hospital between June 2014 and February 2017 were selected as liver cancer group, and healthy subjects who received physical examination in Jianghan Oilfield General Hospital during the same period were selected as control group. Serum was collected from two groups of subjects to determine the contents of GP73, SOD and GPC3;liver cancer lesion and adjacent lesion were collected from liver cancer group to determine the expression of cell proliferation molecules and angiogenesis molecules. Results: Serum GP73 and GPC3 levels of liver cancer group were obviously higher than those of control group while SOD content was obviously lower than that of control group;DNMT3B, STC2, SIRT6, LETM1, EphB4, SULT2B1, HIF-1 , VEGF, Ang-2, HGF and TGF-β1 protein expression levels in liver cancer lesion of liver cancer group were significantly higher than those in adjacent lesion;DNMT3B, STC2, SIRT6, LETM1, EphB4, SULT2B1, HIF-1 , VEGF, Ang-2, HGF and TGF-β1 protein expression levels in liver cancer lesion of liver cancer group were positively correlated with serum GP73 and GPC3 levels, and negatively correlated with serum SOD level. Conclusion: The changes of GP73, SOD and GPC3 levels in the serum of patients with liver cancer are closely related to the cell proliferation and angiogenesis in liver cancer lesion.展开更多
Objective： To detect differential protein expression in malignant and normal liver cell lines in vitro using the SELDI ProteinChip platform, for investigating the pathogenesis of liver cancer. Methods： Two cell line...Objective： To detect differential protein expression in malignant and normal liver cell lines in vitro using the SELDI ProteinChip platform, for investigating the pathogenesis of liver cancer. Methods： Two cell lines, human normal liver cell line L02 and hepatoma cell line SMMC-7721 were cultured routinely, harvested in good condition and lysed. After quantification, the supernatant of the lysate was tested by IMAC3 （Immobilized Mental Affinity Capture） and WCX2 （Weak Cation Exchange） chips on the SELDI-TOF-MS ProteinChip reader. Results： Protein expression differed between the malignant and normal liver cell lines. A total of 20 differentially expressed proteins were found, among which, 7 were captured by the IMAC3 chip and 14 by the WCX2 chip. Peaks at 5,419, 7,979 and 11,265 Da were higher and at 8,103, 8,492, 10,160 and 11,304 Da lower in SMMC-7721 cells by the IMAC3 chip; peaks at 7,517, 7,945 and 7,979 Da were higher and at 5,061, 5,551, 5,818, 7,439, 9,401,10,100, 10,312, 11,621, 11,662, 11,830 and 12,772 Da lower in SMMC-7721 cells by the WCX2 chip. Interestingly, both chips captured the 7,979 Da peak. In addition, the 11,081 Da peak corresponded precisely with the molecular mass of the calcium binding protein S100A10, which may participate in the formation of liver cancer in association with p36. Conclusion： Detecting differential protein expression in malignant and normal liver cell lines using the SELDI ProteinChip platform was simple, sensitive and repeatable. The results we obtained can serve as a basis for investigating the pathogenesis of liver cancer and aid the discovery of new therapeutic targets.展开更多
目的:观察并探讨p16、cyc lin D1基因在原发性肺癌组织中的表达水平及其与肿瘤病理生物学行为等的关系。方法:采用S-P免疫组化方法检测68例原发性肺癌标本中p16、cyc lin D1蛋白的表达。结果:原发性肺癌中p16蛋白表达阳性率显著低于癌...目的:观察并探讨p16、cyc lin D1基因在原发性肺癌组织中的表达水平及其与肿瘤病理生物学行为等的关系。方法:采用S-P免疫组化方法检测68例原发性肺癌标本中p16、cyc lin D1蛋白的表达。结果:原发性肺癌中p16蛋白表达阳性率显著低于癌旁组织(P<0.05);cyc lin D1在原发肺癌中的阳性率显著高于癌旁组织(P<0.05)。二者表达与肿瘤大小、淋巴结转移、病理分级及疾病分期密切相关(P<0.05),且二者表达显著负相关(P<0.05)。结论:在原发型肺癌中存在抑癌基因p16编码蛋白的表达下调以及原癌基因cyc lin D1表达上调,与肿瘤的进展密切相关。提示二者可能通过影响细胞周期调控在肺癌的发生发展过程中起重要作用。展开更多
基金supported by the Key Program for Science and Technology Development of Henan Province [122102310174]the Zoology Key Subject of Henan Province
文摘Objective： To screen and analyze key express sequence tags （ESTs） which were differentially displayed in every period of SD rats＇ primary hepatic carcinoma and reveal the molecular mechanism of carcinogenesis. Methods： Using diethylnitrosamine （DENA） as a cancerigenic agent, animal models with different phases of primary hepatic cancer were constructed in SD rats. Rats were respectively sacrificed at d 14, d 28, d 56, d 77, d 105 and d 112 after the rats received DENA by gavage, then the livers were harvested. One part of the livers was classified according to their pathological changes, while the other was reserved for molecular mechanism studies on hepatocarcinogenesis. The differentially expressed genes were isolated from both normal and morbid tissues by mRNA differential display technique （DDRT-PCR）. After the fragments were sequenced, bioinformatics were .used to analyze the results. Results： Twelve differentially expressed cDNA fragments were obtained. Nine fragments had the homology with known cDNA clones, especially EST-7 was similar to BN/SsNHsdMCW mitochondrion gene and the identity was 100% which suggested EST-7 may be the part of BN/SsNHsdMCW mitochondrion gene. In contrast, other three fragments （EST-1, EST-3 and EST-5） had extremely low identity to any genes registered in GENBANK databases. Conclusions： BN/SsNHsdMCW mitochondrion gene was expressed in different periods of hepatocarcinogenesis. Moreover, EST-I, EST-3 and EST-5 were suggested to contribute to the development of rat hepatocarcinogenesis, and thus may be candidates of new targets of oncogenes or cancer suppressor genes.
文摘Objective To explore the significance of union examination of blood serum liver cancer tracers in the early diagnosis of liver cancer. Methods We observed and compared the level of blood serum liver cancer tracers armor embryo protein (AFP),crag algae glycosidase (AFU),armor embryo protein heteroplasmon (AFPL3) and γ-Gu Anxian transferase (γ-GT) in early time for primary liver cancer patients and hepatitis liver cirrhosis patients and those chronic hepatitis B patients who had liver cancer family history. Results Finally among the 30 patients in the early liver cancer group,23 were positive with AFP,20 with AFU,15 with AFPL3 and 21 with γ-GT. Five were found positive with blood serum AFP,AFPL3,AFU and γ-GT at the same time; 5 with AFP,AFPL3 and γ-GT; 5 with AFP,AFU and AFPL3; 7 with AFP,AFU and γ-GT. By contrast,in the control group,among the 30 hepatitis liver cirrhosis patients and those chronic hepatitis B patients with liver cancer family history,11 were found positive with AFP,3 with AFPL3,12 with AFU and 14 with γ-GT. None of the patients were found positive with union examination of AFP,AFPL3,AFU and γ-GT in the blood serum at the same time. Conclusion The union examination of AFP,AFU,AFPL3 and γ-GT is significant to the early diagnosis of primary liver cancer.
文摘目的分析在诊断原发性肝癌患者时采取血清α-L-岩藻糖苷酶(α-l-fucosidase,AFU)、甲胎蛋白(α-fetoprotein,AFP)、异常凝血酶原Ⅱ(Protein Induced by Vitamin K Absence or Antagonist-Ⅱ,PIVKA-Ⅱ)检测的价值。方法选取2022年8月—2023年8月邳州市中医院收治的83例原发性肝癌患者以及肝硬化患者作为研究对象,依据疾病类型不同,分为良性组(肝硬化患者41例)、观察组(原发性肝癌患者42例),另选择83例健康人群作为对照组。检测3组血清AFU、AFP、PIVKA-Ⅱ指标,分析上述指标水平,同时通过ROC曲线分析单项以及联合检测诊断原发性肝癌的价值。结果两组患者AFU、AFP、PIVKA-Ⅱ指标均显著高于对照组,且观察组AFU、AFP、PIVKA-Ⅱ指标水平高于良性组,差异有统计学意义(P均<0.05)。联合检测时,AUC提升至0.912,灵敏度提升至89.63%,特异度提升至99.10%,高于单独检测。结论在诊断原发性肝癌患者中,采用联合AFU、AFP、PIVKA-Ⅱ检测方式,有较为理想的诊断价值,能够为完善治疗方案奠定基础,可以作为诊断原发性肝癌的有效方法之一。
文摘Objective: To study the correlation of serum GP73, SOD and GPC3 contents with cell proliferation and angiogenesis in liver cancer lesion. Methods: Patients who were diagnosed with primary liver cancer in Jianghan Oilfield General Hospital between June 2014 and February 2017 were selected as liver cancer group, and healthy subjects who received physical examination in Jianghan Oilfield General Hospital during the same period were selected as control group. Serum was collected from two groups of subjects to determine the contents of GP73, SOD and GPC3;liver cancer lesion and adjacent lesion were collected from liver cancer group to determine the expression of cell proliferation molecules and angiogenesis molecules. Results: Serum GP73 and GPC3 levels of liver cancer group were obviously higher than those of control group while SOD content was obviously lower than that of control group;DNMT3B, STC2, SIRT6, LETM1, EphB4, SULT2B1, HIF-1 , VEGF, Ang-2, HGF and TGF-β1 protein expression levels in liver cancer lesion of liver cancer group were significantly higher than those in adjacent lesion;DNMT3B, STC2, SIRT6, LETM1, EphB4, SULT2B1, HIF-1 , VEGF, Ang-2, HGF and TGF-β1 protein expression levels in liver cancer lesion of liver cancer group were positively correlated with serum GP73 and GPC3 levels, and negatively correlated with serum SOD level. Conclusion: The changes of GP73, SOD and GPC3 levels in the serum of patients with liver cancer are closely related to the cell proliferation and angiogenesis in liver cancer lesion.
基金the grant from the National Natural Science Foundation of China(No.30471527, No. 30540075)Mt. Tai Scholar Construction Engineering Foundation
文摘Objective： To detect differential protein expression in malignant and normal liver cell lines in vitro using the SELDI ProteinChip platform, for investigating the pathogenesis of liver cancer. Methods： Two cell lines, human normal liver cell line L02 and hepatoma cell line SMMC-7721 were cultured routinely, harvested in good condition and lysed. After quantification, the supernatant of the lysate was tested by IMAC3 （Immobilized Mental Affinity Capture） and WCX2 （Weak Cation Exchange） chips on the SELDI-TOF-MS ProteinChip reader. Results： Protein expression differed between the malignant and normal liver cell lines. A total of 20 differentially expressed proteins were found, among which, 7 were captured by the IMAC3 chip and 14 by the WCX2 chip. Peaks at 5,419, 7,979 and 11,265 Da were higher and at 8,103, 8,492, 10,160 and 11,304 Da lower in SMMC-7721 cells by the IMAC3 chip; peaks at 7,517, 7,945 and 7,979 Da were higher and at 5,061, 5,551, 5,818, 7,439, 9,401,10,100, 10,312, 11,621, 11,662, 11,830 and 12,772 Da lower in SMMC-7721 cells by the WCX2 chip. Interestingly, both chips captured the 7,979 Da peak. In addition, the 11,081 Da peak corresponded precisely with the molecular mass of the calcium binding protein S100A10, which may participate in the formation of liver cancer in association with p36. Conclusion： Detecting differential protein expression in malignant and normal liver cell lines using the SELDI ProteinChip platform was simple, sensitive and repeatable. The results we obtained can serve as a basis for investigating the pathogenesis of liver cancer and aid the discovery of new therapeutic targets.
文摘目的:观察并探讨p16、cyc lin D1基因在原发性肺癌组织中的表达水平及其与肿瘤病理生物学行为等的关系。方法:采用S-P免疫组化方法检测68例原发性肺癌标本中p16、cyc lin D1蛋白的表达。结果:原发性肺癌中p16蛋白表达阳性率显著低于癌旁组织(P<0.05);cyc lin D1在原发肺癌中的阳性率显著高于癌旁组织(P<0.05)。二者表达与肿瘤大小、淋巴结转移、病理分级及疾病分期密切相关(P<0.05),且二者表达显著负相关(P<0.05)。结论:在原发型肺癌中存在抑癌基因p16编码蛋白的表达下调以及原癌基因cyc lin D1表达上调,与肿瘤的进展密切相关。提示二者可能通过影响细胞周期调控在肺癌的发生发展过程中起重要作用。
