Epigenetic regulation of targeted ferroptosis: A new strategy for drug development

Ferroptosis is a newly discovered form of cell death that is influenced by iron levels and is triggered by cellular metabolism and excessive lipid peroxidation.Epigenetic regulation plays a crucial role in the development and progression of diseases,making it essential to understand these mechanisms in order to identify potential targets for drug development and clinical treatment.The intersection of ferroptosis and epigenetics has opened up new avenues for research in drug development,offering innovative strategies for combating diseases.Recent studies have shown that epigenetic modifications can impact pathways related to ferroptosis,potentially leading to organ dysfunction.Despite the increasing focus on this relationship,the role of epigenetic regulation in drug development remains largely unexplored.This article explores current research on the interplay between epigenetic regulation and ferroptosis,delving into their regulatory mechanisms and discussing the effects of existing epigenetic modification regula-tors on diseases.Additionally,we highlight ongoing research on epigenetic factors involved in targeting ferroptosis in cancer,providing new insights for the development of cancer treatments.

Research on the Development Strategy of Smart Tourism in Hainan Under the Background of Big Data

Hainan is a major tourist province.It is urgent to promote the transformation and upgrading of Hainan’s tourism industry from a traditional service industry to a modern service industry by means of informatization.Smart tourism is a brand-new tourism form and operation mode of tourism transformation and upgrading.Integrating big data technology will make smart tourism more accurate in three aspects:tourism management,tourism service,and tourism marketing,and further enhance the satisfaction of the tourism experience.This paper studies the development status of smart tourism in Hainan,deeply summarizes its existing problems and causes,and puts forward the development strategy of smart tourism in Hainan to promote the healthy development of the tourism industry in Hainan.

Interplay of Development and Governance in China's Modernization Strategy

High-quality development requires both development-oriented and government-oriented reforms.SINCE 1978,China's policy of reform and opening-up has been pivotal in propelling the nation toward big achievements.Its distinct and unique path of development has been a success due to a system and mechanisms that have been continually fine-tuned and strengthened through ongoing reforms.

Analysis of Innovative Paths for Enterprise Marketing Management Development Strategy under the Background of New Media

From the perspective of enterprise marketing strategy planning,innovative research should be conducted based on the current trends in new media.The findings should be applied to practical activities within enterprises to achieve innovation in marketing strategy decision-making in the new media environment.Enterprises should adopt advanced communication transmission technologies and big data processing capabilities to effectively leverage the hidden value and promotional planning advantages of network media platforms.This approach can enhance customer loyalty and increase demand for new products.Enterprises should integrate into the continuously evolving new media landscape,explore their potential business characteristics,and innovate promotional methods.By effectively combining product information with digital media content,companies can ultimately achieve significant increases in corporate interests.

Development Strategy of Sichuan Sports Museum under the Background of Cultural and Tourism Integration

Through conducting a SWOT analysis of the Chengdu Sports Institute Museum,and utilizing the 4M management,5W display,and 4P marketing theoretical models,this study explores effective strategies suitable for Sichuan sports museums in terms of functionality,display,and marketing.

“Hepatic hilum area priority,liver posterior first”:An optimized strategy in laparoscopic resection for type Ⅲ-Ⅳ hilar cholangiocarcinoma

BACKGROUND In recent years,pure laparoscopic radical surgery for Bismuth-Corlette type Ⅲ and Ⅳ hilar cholangiocarcinoma(HCCA)has been preliminarily explored and applied,but the surgical strategy and safety are still worthy of further improvement and attention.AIM To summarize and share the application experience of the emerging strategy of“hepatic hilum area dissection priority,liver posterior separation first”in pure laparoscopic radical resection for patients with HCCA of Bismuth-Corlette types Ⅲ and IV.METHODS The clinical data and surgical videos of 6 patients with HCCA of Bismuth-Corlette types Ⅲ and Ⅳ who underwent pure laparoscopic radical resection in our department from December 2021 to December 2023 were retrospectively analyzed.RESULTS Among the 6 patients,4 were males and 2 were females.The average age was 62.2±11.0 years,and the median body mass index was 20.7(19.2-24.1)kg/m^(2).The preoperative median total bilirubin was 57.7(16.0-155.7)μmol/L.One patient had Bismuth-Corlette type Ⅲa,4 patients had Bismuth-Corlette type Ⅲb,and 1 patient had Bismuth-Corlette type Ⅳ.All patients successfully underwent pure laparoscopic radical resection following the strategy of“hepatic hilum area dissection priority,liver posterior separation first”.The operation time was 358.3±85.0 minutes,and the intraoperative blood loss volume was 195.0±108.4 mL.None of the patients received blood transfusions during the perioperative period.The median length of stay was 8.3(7.0-10.0)days.Mild bile leakage occurred in 2 patients,and all patients were discharged without serious surgery-related complications.CONCLUSION The emerging strategy of“hepatic hilum area dissection priority,liver posterior separation first”is safe and feasible in pure laparoscopic radical surgery for patients with HCCA of Bismuth-Corlette types Ⅲ and Ⅳ.This strategy is helpful for promoting the modularization and process of pure laparoscopic radical surgery for complicated HCCA,shortens the learning curve,and is worthy of further clinical application.

Food Security Problems and Solutions in China Based on the Strategy of Sustainable Agricultural Development

China attaches great importance to food security.With the strong promotion of the government,Chinas grain output has achieved a great feat from"shortage"to"surplus in bumper years"and then to"rising steadily",achieving a record"increase for the tenth consecutive year".From the current balance of food supply and demand in China,the food security situation can be guaranteed on the whole.However,if we analyze the food security in China from the perspective of agricultural sustainable development strategy,there are some difficulties,such as the reduction of cultivated land area,the decline of cultivated land quality,the fragility of agricultural ecological environment,and the transformation of residentsconsumption structure.This paper analyzes the difficulties faced by the development of food security in China under the strategy of sustainable agricultural development,and puts forward that to implement the strategy of sustainable agricultural development,it is necessary to realize the coordinated development of food security,science and technology,ecological environment,resources and society.

Enhanced Deep Reinforcement Learning Strategy for Energy Management in Plug-in Hybrid Electric Vehicles with Entropy Regularization and Prioritized Experience Replay

Plug-in Hybrid Electric Vehicles(PHEVs)represent an innovative breed of transportation,harnessing diverse power sources for enhanced performance.Energy management strategies(EMSs)that coordinate and control different energy sources is a critical component of PHEV control technology,directly impacting overall vehicle performance.This study proposes an improved deep reinforcement learning(DRL)-based EMSthat optimizes realtime energy allocation and coordinates the operation of multiple power sources.Conventional DRL algorithms struggle to effectively explore all possible state-action combinations within high-dimensional state and action spaces.They often fail to strike an optimal balance between exploration and exploitation,and their assumption of a static environment limits their ability to adapt to changing conditions.Moreover,these algorithms suffer from low sample efficiency.Collectively,these factors contribute to convergence difficulties,low learning efficiency,and instability.To address these challenges,the Deep Deterministic Policy Gradient(DDPG)algorithm is enhanced using entropy regularization and a summation tree-based Prioritized Experience Replay(PER)method,aiming to improve exploration performance and learning efficiency from experience samples.Additionally,the correspondingMarkovDecision Process(MDP)is established.Finally,an EMSbased on the improvedDRLmodel is presented.Comparative simulation experiments are conducted against rule-based,optimization-based,andDRL-based EMSs.The proposed strategy exhibitsminimal deviation fromthe optimal solution obtained by the dynamic programming(DP)strategy that requires global information.In the typical driving scenarios based onWorld Light Vehicle Test Cycle(WLTC)and New European Driving Cycle(NEDC),the proposed method achieved a fuel consumption of 2698.65 g and an Equivalent Fuel Consumption(EFC)of 2696.77 g.Compared to the DP strategy baseline,the proposed method improved the fuel efficiency variances(FEV)by 18.13%,15.1%,and 8.37%over the Deep QNetwork(DQN),Double DRL(DDRL),and original DDPG methods,respectively.The observational outcomes demonstrate that the proposed EMS based on improved DRL framework possesses good real-time performance,stability,and reliability,effectively optimizing vehicle economy and fuel consumption.

Measurement and Strategy Research on the Green Development Level for the New Energy Vehicle Industry in Chongqing City

This article takes 2016-2022 as the inspection period to construct an evaluation index system for the green development level of the new energy vehicle industry.The entropy method and comprehensive index are used to measure the green development level of the new energy vehicle industry in Chongqing,and compared with neighboring provinces such as Yunnan,Guizhou,and Sichuan.Policy recommendations are proposed to promote the development of the new energy vehicle industry in Chongqing City.

New aspects of a small GTPase RAB35 in brain development and function

In eukaryotic cells,organelles in the secretory,lysosomal,and endocytic pathways actively exchange biological materials with each other through intracellular membrane trafficking,which is the process of transporting the cargo of proteins,lipids,and other molecules to appropriate compartments via transport vesicles or intermediates.These processes are strictly regulated by various small GTPases such as the RAS-like in rat brain(RAB)protein family,which is the largest subfamily of the RAS superfamily.Dysfunction of membrane trafficking affects tissue homeostasis and leads to a wide range of diseases,including neurological disorders and neurodegenerative diseases.Therefore,it is important to understand the physiological and pathological roles of RAB proteins in brain function.RAB35,a member of the RAB family,is an evolutionarily conserved protein in metazoans.A wide range of studies using cultured mammalian cells and model organisms have revealed that RAB35 mediates various processes such as cytokinesis,endocytic recycling,actin bundling,and cell migration.RAB35 is also involved in neurite outgrowth and turnover of synaptic vesicles.We generated brain-specific Rab35 knockout mice to study the physiological roles of RAB35 in brain development and function.These mice exhibited defects in anxiety-related behaviors and spatial memory.Strikingly,RAB35 is required for the precise positioning of pyramidal neurons during hippocampal development,and thereby for normal hippocampal lamination.In contrast,layer formation in the cerebral cortex occurred superficially,even in the absence of RAB35,suggesting a predominant role for RAB35 in hippocampal development rather than in cerebral cortex development.Recent studies have suggested an association between RAB35 and neurodegenerative diseases,including Parkinson's disease and Alzheimer's disease.In this review,we provide an overview of the current understanding of subcellular functions of RAB35.We also provide insights into the physiological role of RAB35 in mammalian brain development and function,and discuss the involvement of RAB35 dysfunction in neurodegenerative diseases.

Building a Business and Strategic Intelligence Policy as a Strategy for Promoting Congolese Business Progress and Healthy Economic Development in Eastern DRC

The aim of this study was to verify the existence of business and strategic intelligence policies at the level of Congolese companies and at the state level, likely to foster progress and healthy development in the east of the DRC. The study was based on a mixed perspective consisting of objective analysis of quantitative data and interpretative analysis of qualitative data. The results showed that business and strategic intelligence policies have not been established at either company or state level, as this is an area of activity that is not known to the players in companies and public departments, and there are no units or offices in their organizational structures responsible for managing strategic information for competitiveness on the international market. In addition, there is a real need to establish strategic information management units within companies, upstream, and to set up a national strategic information management department or agency to help local companies compete in the marketplace, downstream. This reflects the importance and timeliness of building business and strategic intelligence policies to ensure economic progress and development in the eastern DRC. Business and strategic intelligence provides companies with an appropriate tool for researching, collecting, processing and disseminating information useful for decision-making among stakeholders, in order to cope with a crisis or competitive situation. The study suggests a number of key recommendations based on its findings. To the government, it is recommended to establish the national policy of business and strategic intelligence by setting up a national agency of strategic intelligence in favor of local companies;and to companies to establish business intelligence units in their organizational structures in favor of stakeholders to foster advantageous decision-making in the competitive market and achieve progress. Finally, the study suggests that studies be carried out to fully understand the opportunities and impact of business and strategic intelligence in African countries, particularly in the DRC.

Computational Strategy Research on the Development of Kiruna,in Sweden

In Kiruna,nearly all the residents support the migration plan in order to make LKAB able to continue the mining process.Based on current condition,a larger part of this town will be demolished by the sinking land caused by iron ore mining processing operated by LKAB.Since then,most of the buildings in the sinking district will be relocated in another district.However,the situation in Kiruna is not quite the same as when this kind of migration happens in another place.In other place,while migration happens,even if not most,a large part of the local residents will resist and fight for their house and properties.Concentrated on the ownership of different buildings and tried find the relationship between the public and private buildings to discover some issue that will help to work on the computational urban design.

Research on the Development Strategy of Panxi Sunshine Health and Wellness Homestays and Hotels Based on Traditional Chinese Medicine Health Preservation

This study is the first phase of research results from the student innovation project“Yunqiao Sunshine Health and Wellness Homestay”group at Chengdu Normal University.By analyzing the current state of the Sunshine Health and Wellness Tourism Area economy in the Panxi region of Sichuan,local policies,and the current state of Sunshine Health and Wellness homestays and hotels,this paper proposes the possibility of developing Sunshine Health and Wellness homestays and hotels through traditional Chinese medicine(TCM)health preservation methods.It also proposes development strategies for such hotels through a market survey SWOT analysis.

Establishment of human cerebral organoid systems to model early neural development and assess the central neurotoxicity of environmental toxins

Human brain development is a complex process,and animal models often have significant limitations.To address this,researchers have developed pluripotent stem cell-derived three-dimensional structures,known as brain-like organoids,to more accurately model early human brain development and disease.To enable more consistent and intuitive reproduction of early brain development,in this study,we incorporated forebrain organoid culture technology into the traditional unguided method of brain organoid culture.This involved embedding organoids in matrigel for only 7 days during the rapid expansion phase of the neural epithelium and then removing them from the matrigel for further cultivation,resulting in a new type of human brain organoid system.This cerebral organoid system replicated the temporospatial characteristics of early human brain development,including neuroepithelium derivation,neural progenitor cell production and maintenance,neuron differentiation and migration,and cortical layer patterning and formation,providing more consistent and reproducible organoids for developmental modeling and toxicology testing.As a proof of concept,we applied the heavy metal cadmium to this newly improved organoid system to test whether it could be used to evaluate the neurotoxicity of environmental toxins.Brain organoids exposed to cadmium for 7 or 14 days manifested severe damage and abnormalities in their neurodevelopmental patterns,including bursts of cortical cell death and premature differentiation.Cadmium exposure caused progressive depletion of neural progenitor cells and loss of organoid integrity,accompanied by compensatory cell proliferation at ectopic locations.The convenience,flexibility,and controllability of this newly developed organoid platform make it a powerful and affordable alternative to animal models for use in neurodevelopmental,neurological,and neurotoxicological studies.

Postnatal development of rat retina:a continuous observation and comparison between the organotypic retinal explant model and in vivo development

The organotypic retinal explant culture has been established for more than a decade and offers a range of unique advantages compared with in vivo experiments and cell cultures.However,the lack of systematic and continuous comparison between in vivo retinal development and the organotypic retinal explant culture makes this model controversial in postnatal retinal development studies.Thus,we aimed to verify the feasibility of using this model for postnatal retinal development studies by comparing it with the in vivo retina.In this study,we showed that postnatal retinal explants undergo normal development,and exhibit a consistent structure and timeline with retinas in vivo.Initially,we used SOX2 and PAX6 immunostaining to identify retinal progenitor cells.We then examined cell proliferation and migration by immunostaining with Ki-67 and doublecortin,respectively.Ki-67-and doublecortin-positive cells decreased in both in vivo and explants during postnatal retinogenesis,and exhibited a high degree of similarity in abundance and distribution between groups.Additionally,we used Ceh-10 homeodomain-containing homolog,glutamate-ammonia ligase(glutamine synthetase),neuronal nuclei,and ionized calcium-binding adapter molecule 1 immunostaining to examine the emergence of bipolar cells,Müller glia,mature neurons,and microglia,respectively.The timing and spatial patterns of the emergence of these cell types were remarkably consistent between in vivo and explant retinas.Our study showed that the organotypic retinal explant culture model had a high degree of consistency with the progression of in vivo early postnatal retina development.The findings confirm the accuracy and credibility of this model and support its use for long-term,systematic,and continuous observation.

Current Status and Development Strategy of Mung Bean Production in Chongqing Municipality

[Objective] This paper presented the current status of mung bean produc- tion in Chongqing, and put forward the corresponding development strategies. [Meth- ods] By overviewing the status of mung bean production in Chongqing Municipality, the main problems, advantages of mung bean production in Chongqing were pre- sented, and based on corresponding development strategies were put forward.[Re- suits] Currently, the main problems of mung bean production in Chongqing area in- clude insufficient attention, serious variety degradation, lag in cultivation technology, low scientific research level. However, the region has strengths in growing mung beans which would have broad prospects by appropriate adjustment and planning. [Conclusion] This study laid the foundation for the development of mung bean pro- duction and promotion of economic development in Chongqing Municipality.

Current situation of Chinese forestry tactics and strategy of sustainable development

Based on the analysis of the current situation of Chinese forestry and a series of ecological, environmental, eco-nomic, and social problems, this paper emphasized on the importance of forests in social and economic development in China The author pointed out that sustainable development, particularly the sustainable development of forestry, is the inevitable choice to solve the problems of ecological environment and social economics. The objectives, criteria, and strategies of sus-taining forestry were also demonstrated and expounded, from the viewpoints of ecology, economics, and sociology.

Research on Development Strategy of Tourism Supporting for Poverty Alleviation in West Hubei

West Hubei Circle referred to the eight cities and prefectures which were Xiangfan,Jingzhou,Yichang,Shiyan,Jingmen,Suizhou,Enshi and Shennongjia.The economic and social development in this area was lagging behind,with wide poverty area,large impoverished population,poor natural condition and weak development foundation.Owing to lack of investment sources,the poverty-overcoming cost and poverty-returning rate in this area was high.It was the key area of poverty alleviation of Hubei Province.On the basis of analysis of the features of poverty alleviation in poverty-stricken areas of West Hubei,with the internal relation between tourism and poverty-alleviation as the reference,the author proposed the conceptualization of linkage development of tourism and poverty-alleviation west Hubei and preliminarily analyzed the feasibility of the linkage development between tourism and poverty-alleviation.The author considered that tourism and poverty-alleviation could mutually complement each other in function.The cultural and ecological tourism resources in West Hubei were abundant and unique,high in resources taste.And tourism was an industry with high return and intensive capital.It could collect capitals,lands,talents and technologies,win a great deal of human,material,capital and information resources in West Hubei,so as the promote the amelioration of the rural infrastructure and living conditions and urban construction.Meanwhile,the basic strategy of poverty-alleviation was put forward,that is,establishing the development mechanism with government as the lead and enterprises as the subjects,the financing mechanism of diversified investments and wide-ranging financing.Besides,with tourism as the trigger,tourism supporting for poverty-alleviation and ecological cultural village would be developed simultaneously,assisted by community participation mechanism,so as to realize mutual benefit and win-win cooperation of tourism and poverty-alleviation.

Meta-cognitive Strategy Training and the Development of Learners＇ Autonomy in Language Learning

As the ultimate goal of education, autonomy in language learning has aroused a lot of attention from scholars at home and abroad. While in universities of China, students do not have strong autonomy in English language learning. The author tries to adopt specific meta-cognitive strategies to facilitate students＇ autonomy in learning by improving learners＇ capacities in study planning or management, monitoring and evaluating in learning to raise their consciousness and ability in autonomy, and lay a foundation for life-long learning.

Study on Environmental Capacity and Sustainable Development Strategy of Scenic Areas ——Taking the Scenic Area of Slender West Lake in Yangzhou for an Example

Taking the scenic area of Slender West Lake in Yangzhou for an example, the quantitative evaluation model of environmental capacity and impact factors limiting sustainable development of scenic area were put forward, and targeted control measures were correspondingly proposed.