Octadecylamine was derivatized with dansyl chloride (5-dimethylaminonaphthalene-1-sulfonyl chloride) In order to simplify and understand the LB films of fluorescent probe labeling proteins. its monolayer and multilaye...Octadecylamine was derivatized with dansyl chloride (5-dimethylaminonaphthalene-1-sulfonyl chloride) In order to simplify and understand the LB films of fluorescent probe labeling proteins. its monolayer and multilayers in the absence and presence of stearic acid were deposited by LB technique. Fluorescence spectra and lifetimes of the fluorescent products were studied to elucidate the microenvironment of molecules in the LB films.展开更多
A commercially available non—radioactive DNA labelling kit "enhanced chemiluminescence"(ECL) was evaluated for the diagnosis of falciparum malaria. The results showed that ECL labeled probe successfully det...A commercially available non—radioactive DNA labelling kit "enhanced chemiluminescence"(ECL) was evaluated for the diagnosis of falciparum malaria. The results showed that ECL labeled probe successfully detected as little as 25 pg. Purified DNA of 0.001％ parasitemia of cultured Plasmodium falciparum and did not react with human展开更多
A new rapid technique for intrahepatic and extrahepatic HBV DNA detection by using digoxigenin labelled probe with in situ hybridization was developed.This technique has the advantage of being non-radioactive and a qu...A new rapid technique for intrahepatic and extrahepatic HBV DNA detection by using digoxigenin labelled probe with in situ hybridization was developed.This technique has the advantage of being non-radioactive and a quick procedure yielding stable results and showing a clear background.展开更多
We report on a novel dibenzothiophene-based two-photon fluorescent probe for selective nuclear bioimaging,which contains bilaterally symmetrical pyridine rings connected by a central conjugated-bridge dibenzothiophene...We report on a novel dibenzothiophene-based two-photon fluorescent probe for selective nuclear bioimaging,which contains bilaterally symmetrical pyridine rings connected by a central conjugated-bridge dibenzothiophene. This probe possesses a large two-photon absorption cross-section of 471 GM, yields a 25-fold enhancement of the fluorescence titration, and a stronger photostability for nuclei labeling than existing probes. The real-time observation period is a minimum of 1800 s under a femtosecond laser excitation, which is significantly longer than that of 40,6-diamidino-2-phenylindole. The above results confirm that this novel molecule is a suitable two-photon fluorescent probe for application to nuclear bioimaging in cells.展开更多
基金the National Natural Science Foundation of China (296333010) and The President Science Foundation of the Chinese Academy of Scie
文摘Octadecylamine was derivatized with dansyl chloride (5-dimethylaminonaphthalene-1-sulfonyl chloride) In order to simplify and understand the LB films of fluorescent probe labeling proteins. its monolayer and multilayers in the absence and presence of stearic acid were deposited by LB technique. Fluorescence spectra and lifetimes of the fluorescent products were studied to elucidate the microenvironment of molecules in the LB films.
文摘A commercially available non—radioactive DNA labelling kit "enhanced chemiluminescence"(ECL) was evaluated for the diagnosis of falciparum malaria. The results showed that ECL labeled probe successfully detected as little as 25 pg. Purified DNA of 0.001％ parasitemia of cultured Plasmodium falciparum and did not react with human
文摘A new rapid technique for intrahepatic and extrahepatic HBV DNA detection by using digoxigenin labelled probe with in situ hybridization was developed.This technique has the advantage of being non-radioactive and a quick procedure yielding stable results and showing a clear background.
基金supported by the National Natural Science Foundation of China under Grant Nos.61137002,61178059,and 51172253
文摘We report on a novel dibenzothiophene-based two-photon fluorescent probe for selective nuclear bioimaging,which contains bilaterally symmetrical pyridine rings connected by a central conjugated-bridge dibenzothiophene. This probe possesses a large two-photon absorption cross-section of 471 GM, yields a 25-fold enhancement of the fluorescence titration, and a stronger photostability for nuclei labeling than existing probes. The real-time observation period is a minimum of 1800 s under a femtosecond laser excitation, which is significantly longer than that of 40,6-diamidino-2-phenylindole. The above results confirm that this novel molecule is a suitable two-photon fluorescent probe for application to nuclear bioimaging in cells.
