期刊文献+
共找到11篇文章
< 1 >
每页显示 20 50 100
Procaine Inhibiting Human Bladder Cancer Cell Proliferation by Inducing Apoptosis and Demethylating APAF1 CpG Island Hypermethylated 被引量:3
1
作者 SUN Ran CHANG Li-ping +2 位作者 WANG Kai-chen SUN Hong-yan CONG Xian-ling 《Chemical Research in Chinese Universities》 SCIE CAS CSCD 2012年第6期1017-1021,共5页
Studies have shown that aberrant DNA methylation of apoptotic protease activating factor-1(APAF1) is an important epigenetic mechanism of gene regulation in the progression of bladder cancer.In this article,we have ... Studies have shown that aberrant DNA methylation of apoptotic protease activating factor-1(APAF1) is an important epigenetic mechanism of gene regulation in the progression of bladder cancer.In this article,we have proved that procaine,an inhibitor of DNA methyltransferases,could inhibit the proliferation of T24 and 5637 human bladder cancer cells by inducing their apoptosis.The mechanism studies reveal that procaine could induce demethylation of APAF1 gene in T24 or 5637 cells,subsequently activating caspase-3/9.It was also shown that the serum soluble fas ligand(sFasL) was activated,and the expression of matrix metallopeptidase 9(MMP-9) was down-regulated.Procaine seems to induce cell death by different pathways,and it might be used as a potential agent for bladder cancer treatment. 展开更多
关键词 Bladder cancer cell EPIGENETICS DNA methylation Apoptotic protease activating factor-1(APAF1) procaine
下载PDF
Changes in erythrocyte membrane ATPases and plasma lipid peroxides in upper abdominal surgery under intravenous procaine-balanced anesthesia 被引量:2
2
作者 TU Wei Feng 1, LIN Gui Fang 2, SHEN Jian Fan 2 and XU Jian Guo 1 《World Journal of Gastroenterology》 SCIE CAS CSCD 1998年第5期67-70,共4页
AIM To observe the changes in erythrocyte membrane ATPases and plasma lipid peroxides (LPO) patients with in abdominal surgery under intravenous procaine-balanced anesthesia.METHODS By determining the ATPase activitie... AIM To observe the changes in erythrocyte membrane ATPases and plasma lipid peroxides (LPO) patients with in abdominal surgery under intravenous procaine-balanced anesthesia.METHODS By determining the ATPase activities of erythrocyte membrane, effects of upper abdominal surgery under intravenous procaine-balanced anesthesia on the function of erythrocytes were observed in 15 patients undergoing cholecystectomy and gastrectomy (5 males and 10 females, aged 45.9±10.20 years and weighed 60.60kg±11.93kg). All patients were free from severe renal, hepatic, pulmonary, cardiac, metabolic and endocrinological diseases and acute infection for at least 2 weeks before surgery. Patients receiving any drug known to affect carbohydrate metabolism prior to anesthesia were excluded from the study.RESULTS Erythrocyte membrane Na+, K+-ATPase, Mg2+-ATPase, Ca2+, Mg2+-ATPase activities were not significantly changed 60min-90min after incision as compared with 30min before anesthesia, but were decreased markedly 10min and 24 hours after completion of operation (P<0.01). Plasma lipid peroxides (LPO) were increased significantly 24 hours after surgery (P<0.01) following an initially marked but transient reduction. Plasma LPO changes were not correlated with erythrocyte membrane ATPase activities, r=-0.0396, -0.0097 and 0.4383, respectively (P>0.05).CONCLUSION Abdominal surgical trauma under intravenous procaine-balanced anesthesia may be associated with the decreased ATPase activities of erythrocyte membrane and increased LPO in plasma. 展开更多
关键词 ERYTHROCYTE membrane/enzymology anesthesia procaine Ca 2+ Mg 2+ ATPASE Na + K + ATPASE lipid PEROXIDES
下载PDF
Reactive Precipitation of Procaine Benzylpenicillin
3
作者 陆杰 王静康 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2000年第1期68-73,共6页
The reactive precipitation process of procaine benzylpenicillin is reviewed, while such secondary processes as ageing, agglomeration, breakage, and the effects of operation parameters on crystal size are emphasized. I... The reactive precipitation process of procaine benzylpenicillin is reviewed, while such secondary processes as ageing, agglomeration, breakage, and the effects of operation parameters on crystal size are emphasized. In the reactive precipitation the ageing of particles has a little effect on the process, while the greater effect comes from the agglomeration and breakage of particles, furthermore, the mixing has also notable influence on the product size. All of these provide the bases for further study on reactive precipitation. 展开更多
关键词 procaine benzylpenicillin reactive precipitation secondary processes MIXING
下载PDF
PURGING OF ACUTE NON-LYMPHOCYTIC LEUKEMIA PROGENITORS BY PROCAINE AND HYPERTHERMIA
4
作者 荣跃 夏学鸣 林宝爵 《Chinese Journal of Cancer Research》 SCIE CAS CSCD 1993年第1期73-76,共4页
Procaine and hyperthermia have been shown to possess a relatively selective cytotoxicity to leukemlc cells. In this study, the combined effects of procaine and hyperthermia on the growth of hematopoietic progenitors (... Procaine and hyperthermia have been shown to possess a relatively selective cytotoxicity to leukemlc cells. In this study, the combined effects of procaine and hyperthermia on the growth of hematopoietic progenitors (GM-CFU) and ieukemic progenitors (L-CFU) were examined to determine if this combination resulted in a great selective killing of leukemlc cells than that achieved by procaine or heat alone. When the cells were treated simutaneously with procaine (2 mM) and hyperthermia (42℃) for one hour, the killing of L-CFU was enhanced considerably whereas GM-CFU were not markedly affected. These data Indicate that the combined treatment with procain and hyperthermia might offer an efficient mean to selectively purge residual leukemlc cells in vitro. Procaine with hyperthermia may have a role in clinical autoiogous bone marrow transplantation for acute leukemia. 展开更多
关键词 procaine Hyperthermies Acute non- lymphocytic leukemia Purge.
下载PDF
Liquid-liquid Extraction System Based on Non-ionic Surfactant-salt-H_2O and Mechanism of Drug Extraction 被引量:3
5
作者 LeiLI FengLIU 《Chinese Chemical Letters》 SCIE CAS CSCD 2002年第4期349-350,共2页
Extraction behavior of chlorpromazine hydrochloride (CPZ) and procaine hydro- chloride (PCN) in the system described in the title was studied. Research shows that the extraction efficiency of CPZ can amount to 96% by... Extraction behavior of chlorpromazine hydrochloride (CPZ) and procaine hydro- chloride (PCN) in the system described in the title was studied. Research shows that the extraction efficiency of CPZ can amount to 96% by twice extraction, while that of PCN is 77%. This system produces the distribution coefficients (KD) of 12.3 and 2.6 respectively for CPZ and PCN. Extraction mechanism is deduced according to ultraviolet and molecular fluorescence spectra variation of the drugs in the system studied. 展开更多
关键词 Non-ionic surfactant-salt-H2O extraction system polyethylene glycol-1000 chlor- promazine hydrochloride procaine hydrochloride mechanism of extraction.
下载PDF
Seventy Cases of External Humeral Epicondylitis Treated by Local Blocking and Massotherapy
6
作者 王兴峰 周永生 《Journal of Traditional Chinese Medicine》 SCIE CAS CSCD 2001年第1期52-53,共2页
External humeral epicondylitis or tennis elbow, is a commonly encountered disease in orthopaedics and traumatology. The curative effect is not satisfactory as far as its treatment by western and traditional Chinese me... External humeral epicondylitis or tennis elbow, is a commonly encountered disease in orthopaedics and traumatology. The curative effect is not satisfactory as far as its treatment by western and traditional Chinese medicine is concerned, which has a long course of treatment and a high recurrent rate. 70 cases of external humeral epicondylitis were treated by massotherapy after local blocking from August 1995 to October 1997 at this hospital with satisfactory therapeutic effects.Clinical DataOf the 70 cases in this series treated by massotherapy after local blocking, 30 were males and 40 females, ranging in age from 19 to 65 years. 55 cases were 30 to 50 years old, 20 had the left elbow affected and 50 the right elbow. 7 cases had a history of trauma, 50 a history of chronic strain, and 13 the cause unknown. The shortest duration of disease was 15 days and the longest 24 months.Among 50 cases in the control group treated by massotherapy, 20 cases were males and 30 females, ranging in age from 16 to 58 years. 40 cases were 30 to 50 years old. 15 cases were affected on the left elbow and 35 on the right elbow. 6 cases had a history of trauma, 32 a history of chronic strain, and 12 the cause unknown. The shortest duration of diseases was 7 days and the longest 22 months. 展开更多
关键词 MASSAGE ADULT Aged Combined Modality Therapy FEMALE Humans MALE Middle Aged Nerve Block procaine Tennis Elbow Triamcinolone Acetonide
下载PDF
16 CASES OF SCALENUS SYNDROME TREATED BY MASSAGE AND ACUPOINT-INJECTION
7
作者 彭浚宇 《Journal of Traditional Chinese Medicine》 SCIE CAS CSCD 1999年第3期218-220,共3页
Scalenus syndrome is a kind of thoracicoutlet syndrome. I treated 16 such cases withmassage in combination with acupointinjection from 1989-1996. The results weresatisfactory and introduced as follows.
关键词 Acupuncture Points ADULT Combined Modality Therapy FEMALE Humans INJECTIONS MASSAGE procaine Thoracic Outlet Syndrome Vitamin B 12
下载PDF
Effect of post-burn serum on free cytosolic calcium in isolated cardiac myocytes of adult rats 被引量:1
8
作者 沈洪兴 方勇 +3 位作者 陈玉林 刘世康 葛绳德 夏照帆 《Journal of Medical Colleges of PLA(China)》 CAS 1998年第1期58-62,共5页
The ohjective of this study was to determine whether the free intracellular calcium concentration ([Ca2+] ) of isolatedcardiac myocytes increased with the stimulation of post-burn serum(PBS) in adult rats. Cardiac myo... The ohjective of this study was to determine whether the free intracellular calcium concentration ([Ca2+] ) of isolatedcardiac myocytes increased with the stimulation of post-burn serum(PBS) in adult rats. Cardiac myocytes were isolated by collage-nase using Langendorff’s perfusion apparatus, and [Ca2+], was measured using the fluorescent indicator Fain-2. The normal[Ca2+], was 101. 3 ± 21. 3 nmol/L in cardic myocytes. PBS at various postburn home could very significantly increase the[Ca2+]i (P< 0. 01 ) and, 6 h PBS had the strongest effect. However, no significant difference was found between the effects of2 h PBS and 4 h PBS (P >0. 05 ). Both calcium channel antagonist verapamil(30 umol/L) and the inhibitor of ryanodine receptoron sarcoplasmic reticulum procaine (2 mmol/L), very significantly inhibited the action of 6 h PBS, with the inhibition rate of47. 7% and 67. 6% respectively. The inhibiting rate of procaine was significantly greater than that of verapamil (P < 0. 01 ). Theresults suggested that PBS could stimulate the increase of [Ca2+], in isolated cardiac myocytes of adult rats, in which calcium release from intracellular stores might play greater roles. Agents modulating the calcium release from intracellular stores are expectedto have great significance in preventing the organic injuries due to the increases of [ Ca2+]i. 展开更多
关键词 BURNS calcium CARDIAC MYOCYTE procaine
全文增补中
A quadruplex immunochromatographic assay for the ultrasensitive detection of 11 anesthetics 被引量:4
9
作者 Xianlu Lei Xinxin Xu +5 位作者 Li Wang Wei Zhou Liqiang Liu Liguang Xu Hua Kuang Chuanlai Xu 《Nano Research》 SCIE EI CSCD 2023年第8期11269-11277,共9页
Anesthetic residues in fish represent a potential risk to human health.Therefore,it is important to develop a sensitive and broad-specific method for the detection of anesthetics.In this study,we developed a colloidal... Anesthetic residues in fish represent a potential risk to human health.Therefore,it is important to develop a sensitive and broad-specific method for the detection of anesthetics.In this study,we developed a colloidal gold-based quadruplex immunochromatographic(Qua-ICS)assay using four highly sensitive monoclonal antibody immunotherapy(mAbs)that simultaneously detected 11 anesthetic residues in fish within 10 min.The colorimetric and cut-off values(COVs)for procaines,eugenols,bupivacaines,and tricaine(TMS)were 0.37–1.1 and 3.3–10,11–222 and 100–2000,0.37 and 3.3,and 111 and 10,000µg/kg,respectively.Quantitative analysis was achieved with a portable strip-reader,and the detection ranges were 0.15–2.6,6.3–677,0.13–2.8,and 83–1245µg/kg for procaines,eugenols,bupivacaines,and tricaine,respectively.Our developed method was reliable and accurate according to the recovery test results and analyses of real samples.Therefore,the strip can be used as an alternative method for the rapid detection of anesthetic residues in fish. 展开更多
关键词 BUPIVACAINE colloidal gold EUGENOL procaine tricaine(TMS)
原文传递
The effects of local anesthetics on intracellular Ca2+ release from ryanod ine-sensitive Ca2+ stores in gerbil hippocampal neurons
10
作者 陈俊峰 徐伟人 蒋豪 《Chinese Medical Journal》 SCIE CAS CSCD 2002年第10期1542-1544,共3页
OBJECTIVE: To examine the effects of procaine and lidocaine on intracellular Ca(2+) release from sarcoplasmic reticulum ryanodine-sensitive Ca(2+) stores. METHODS: The experiment was performed on hippocampal slices fr... OBJECTIVE: To examine the effects of procaine and lidocaine on intracellular Ca(2+) release from sarcoplasmic reticulum ryanodine-sensitive Ca(2+) stores. METHODS: The experiment was performed on hippocampal slices from 60-80 g male Mongolian gerbils. Levels of intracellular Ca(2+) concentration in the slices were measured by microfluorometry. The slices were perfused with 50 mmol/L KCl containing medium for 30 seconds. Then, the medium was switched to physiological medium. After 5 min of incubation, the slice was perfused with 20 mmol/L caffeine containing physiology medium for 2 min. Following incubation, the slice was superfused with physiological medium until the end of the experiment. The effects of procaine and lidocanin (100 micro mol/L) on caffeine-evoked Ca(2+) release were evaluated by adding them to the medium after high K(+) medium perfusion. RESULTS: Caffeine induced a marked increase in intracellular Ca(2+) concentration which was then decreased 12% upon the addition of procaine (P 展开更多
关键词 Anesthetics Local Animals Calcium GERBILLINAE Hippocampus LIDOCAINE Male procaine RYANODINE Ryanodine Receptor Calcium Release Channel
原文传递
The effects of local anesthetics on intracellular Ca^(2+) release from ryanodine-sensitive Ca^(2+) stores in gerbil hippocampal neurons
11
作者 陈俊峰 徐伟人 蒋豪 《Chinese Medical Journal》 SCIE CAS CSCD 2002年第10期102-104,153-154,共5页
To examine the effects of procaine and lidocaine on intracellular Ca 2+ release from sarcoplasmic reticulum ryanodine sensitive Ca 2+ stores Methods The experiment was performed on hippocampal slices fro... To examine the effects of procaine and lidocaine on intracellular Ca 2+ release from sarcoplasmic reticulum ryanodine sensitive Ca 2+ stores Methods The experiment was performed on hippocampal slices from 60-80 g male Mongolian gerbils Levels of intracellular Ca 2+ concentration in the slices were measured by microfluorometry The slices were perfused with 50 mmol/L KCl containing medium for 30 seconds Then, the medium was switched to physiological medium After 5 min of incubation, the slice was perfused with 20 mmol/L caffeine containing physiology medium for 2 min Following incubation, the slice was superfused with physiological medium until the end of the experiment The effects of procaine and lidocanin (100 μmol/L) on caffeine evoked Ca 2+ release were evaluated by adding them to the medium after high K + medium perfusion Results Caffeine induced a marked increase in intracellular Ca 2+ concentration which was then decreased 12% upon the addition of procaine ( P 【0 05); however, lidocaine, did not induce a similar inhibitory reaction Conclusion Procaine inhibits ryanodine receptor mediated Ca 2+ release from intracellular Ca 2+ stores, while lidocaine may inhibit Ca 2+ release through other mechanisms 展开更多
关键词 procaine · lidocaine · caffeine · gerbi l · hippocampus
全文增补中
上一页 1 下一页 到第
使用帮助 返回顶部