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CAx Process Chain for Automated Laser Drilling of Tool Molds
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作者 Hermann Uchtmann Ingomar Kelbassa 《Journal of Mechanics Engineering and Automation》 2014年第5期427-431,共5页
Rotation sintering, also known as slush molding, is used to manufacture molded skins, such as dashboards or door interior panels for cars. At present, approximately 80% of such molded skins are manufactured using elec... Rotation sintering, also known as slush molding, is used to manufacture molded skins, such as dashboards or door interior panels for cars. At present, approximately 80% of such molded skins are manufactured using electroforms to achieve the complex free-form surfaces, and surface structures, such as leather graining that the industry demands. The manufacture of these electroforms is, however, time-consuming and expensive. This project aims to replace conventional electroforms with laser-drilled molds. Holes in tool molds should be drilled by using laser radiation as part of an automated process. The system consists of a robot with a fiber-laser beam source. A CAx (computer-aided x) process chain has been developed for this purpose in which the CAD (computer-aided design) data of the tool molds are processed, drill hole fields generated, and a machine-specific RC (robot control) program created. Process-specific fundamentals, such as suitable process windows and process control, have been devised to manufacture holes using fiber laser radiation The advantages of the new laser-drilled tool molds may result in substituting them for conventional electroforms, allowing old markets to be re-entered or additional markets to be created and targeted through new molds or lower costs. 展开更多
关键词 Laser drilling fiber lasers tool molds CAx process chain.
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Forming process chain for manufacturing complex conical-section profiled rings:On a co-design method
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作者 Lei LIANG Chaojiang ZOU Lianggang GUO 《Chinese Journal of Aeronautics》 SCIE EI CAS CSCD 2024年第6期485-503,共19页
The final quality of complex conical-section rings depends on co-design of multiple processes in forming process chain.In this study,for a complex aeroengine casing ring with a large slope and a flange on its end,a co... The final quality of complex conical-section rings depends on co-design of multiple processes in forming process chain.In this study,for a complex aeroengine casing ring with a large slope and a flange on its end,a co-design method of the forming process chain is put forward towards the objective of precision forming,which not only proposes a standard process route composed of multiple processes of upsetting,punching,rectangular ring rolling,loose tooling forging and profiled ring rolling,but also presents co-design methods of dies and blanks for all the processes.For profiled ring rolling,a design method of preformed blank that makes the blank and the target conical-section ring have the same axial volume distribution is proposed.By the method,the axial metal redistribution during the process can be alleviated greatly thus improving the forming stability and precision of the ring.Based on the geometric features of designed preformed blank,design methods of blanks and dies for loose tolling forging,rectangular ring rolling,punching and upsetting are proposed sequentially.In view of the key roles of loose tooling forging(manufacturing the preformed blank)and profiled ring rolling on the final quality of the conical ring parts,inherited FE simulations for these two processes are performed to verify the proposed design methods and determine appropriate design parameter.It is demonstrated that the proposed design method has significant advantages in improving forming precision.Besides,a suggestive value 1.5 of the rolling ratio for profiled ring rolling(a key design parameter)is given based on comprehensive consideration of multiple indicators such as ring roundness,deformation uniformity and forming load.The corresponding industrial experiments performed illustrate that a high forming precision of the conical-section aeroengine casing ring is achieved. 展开更多
关键词 Complex conical-section ring Co-design of forming process chain Profiled ringrolling Preformed blank Loose tooling forging
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E-Commerce Supply Chain Process Optimization Based on Whole-Process Sharing of Internet of Things Identification Technology 被引量:3
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作者 Shiyan Xu Jun Chen +1 位作者 Maoguo Wu Chenyang Zhao 《Computer Modeling in Engineering & Sciences》 SCIE EI 2021年第2期843-854,共12页
With in-depth development of the Internet of Things(IoT)in various industries,the informatization process of various industries has also entered the fast lane.This article aims to solve the supply chain process proble... With in-depth development of the Internet of Things(IoT)in various industries,the informatization process of various industries has also entered the fast lane.This article aims to solve the supply chain process problem in e-commerce,focusing on the specific application of Internet of Things technology in e-commerce.Warehousing logistics is an important link in today’s e-commerce transactions.This article proposes a distributed analysis method for RFID-based e-commerce warehousing process optimization and an e-commerce supply chain management process based on Internet of Things technology.This article first introduces the advantages and disadvantages of shared IoT identification technology and the IoT resource sharing platform based on the three-layer abstract data model and representational state transfer(REST)style.Combining actual IoT applications and the characteristics of an existing platform,a REST-based IoT resource sharing platform is proposed.Combined with actual projects,a REST-based IoT resource sharing platform was built,and key technology experiments were conducted for verification.Finally,optimizing the e-commerce supply chain management process under Internet of Things technology and explaining the advantages of optimized e-commerce supply chain management are discussed.Research on this subject provides a theoretical basis for the application of the Internet of Things in e-commerce and has practical significance and practical value for managing service capabilities and service levels in e-commerce. 展开更多
关键词 Shared IoT identification technology E-COMMERCE supply chain process optimization
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Optimization of Preservation on Fresh-cut Yam under Simulated Cold-chain Process 被引量:1
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作者 Dongdi XIE Rongling HUANG 《Agricultural Biotechnology》 CAS 2020年第5期103-106,共4页
[Objectives]This study was conducted to investigate the effect of compound fresh-keeping liquid on the browning and quality of fresh-cut yam during the cold chain process.[Methods]Under the simulated cold chain condit... [Objectives]This study was conducted to investigate the effect of compound fresh-keeping liquid on the browning and quality of fresh-cut yam during the cold chain process.[Methods]Under the simulated cold chain condition of 4℃,a uniform design(U6*(64))experiment was carried out to optimize the compound preservative formula,and a better compound preservative formula was obtained using the regression equation.[Results]The compound preservative formula was:AA 0.25%,CA 0.5%,Nisin 0.09%,GSH 0.15%.The compound fresh-keeping liquid effectively delayed the browning of fresh-cut yam and the accumulation of MDA,maintained the content of TSS,inhibited the activity of PAL and delayed the appearance of the maximum activity of POD and POD.[Conclusions]This study provides technical support for the actual extension of the shelf life of fresh-cut yam. 展开更多
关键词 Fresh-cut yam Cold chain process Uniform design PRESERVATION
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The Framework Model of Supply-Chain Process in CALS Environment 被引量:1
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作者 HU Jian-yang, WANG Zhi-ge, MA Shi-hua, SHEN Ling (School of Management, Huazhong University of Science & Technology, W uhan 430074, China ) 《厦门大学学报(自然科学版)》 CAS CSCD 北大核心 2002年第S1期270-271,共2页
E-supply chain is the trend of the supply chain ma na gement. However, building E-supply chain depends on business process improvemen t and re-engineering, which becomes one of the important aspects in supply chai n m... E-supply chain is the trend of the supply chain ma na gement. However, building E-supply chain depends on business process improvemen t and re-engineering, which becomes one of the important aspects in supply chai n management. However, the supply chain process re-engineering and improvement is constrained because of lack of integrating operation framework and standards based on information management. We find that the idea of CALS origins from DoD of USA can solve the problems very well. CALS is the abbreviation of "continues acquisition and life- cycle support". It is an information strategy adopted by many developed counties. The final objective of the CALS is that government a nd industry can generate, exchange, manage and use all the digital information t hroughout the lifecycle of a product in virtue of adopting the international and national standards, improving existing business process and introducing advance d technology. The Integrated Digital Environment (IDE) created by CALS provides a brand new operation mode for consumer, contractor and supplier in supply chain . Besides, a series mutual standard is involved in CALS system. These standards and the standardization technology would be applied widely in the supply chain. This study mainly focuses on supply chain process in CALS environment, and puts forward a framework model of supply chain process. Then the study analyzes the c haracteristics of the model, which includes the application of standards and inf ormation technology, and finally discusses on methods to achieve the supply chai n process in CALS environment. 展开更多
关键词 supply chain business process re-engineering C ALS
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Process Reengineering of Cold Chain Logistics of Agricultural Products Based on Low-carbon Economy 被引量:7
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作者 GUO Hong-xia SHAO Ming 《Asian Agricultural Research》 2012年第2期59-62,共4页
Through the process analysis of cold chain logistics of agricultural products,we find that cold chain logistics of agricultural products contradict the development model of low-carbon economy to some extent.We apply t... Through the process analysis of cold chain logistics of agricultural products,we find that cold chain logistics of agricultural products contradict the development model of low-carbon economy to some extent.We apply the development idea of low-carbon economy,introduce the thirdparty logistics companies,establish distribution center of cold chain logistics of agricultural products,and strengthen information sharing,to reengineer the process of cold chain logistics of agricultural products in China.The results show that applying low-carbon economy to process reengineering of cold chain logistics of agricultural products,has advantages of increasing added value of products,promoting scale merit and abating lag,plays a role in promoting emission reduction,high efficiency and environmental protection in the process of cold chain logistics of agricultural products in China. 展开更多
关键词 Low carbon economy Business process reengineering Agricultural products Cold chain logistics
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Modeling and Analysis for Supply Chain Using Stochastic Process Algebra
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作者 Yong-Tao Huang Min Lv +2 位作者 Gang Wang Bing-Yin Ren Hao-Yun Zhang 《Journal of Harbin Institute of Technology(New Series)》 EI CAS 2014年第6期74-80,共7页
In order to improve the influence of the uncertain and dynamic of node enterprise behavior on the performance of supply chain,the method based on stochastic process algebra for description,analysis,validation and eval... In order to improve the influence of the uncertain and dynamic of node enterprise behavior on the performance of supply chain,the method based on stochastic process algebra for description,analysis,validation and evaluation of supply chain business process model is proposed.Firstly,the description of the uncertainty of node enterprise behavior is given using the extended Unified Modeling Language sequence diagram,and mapping rule is defined from the extended Unified Modeling Language sequence diagram to stochastic process algebra.Secondly,on the basis of the acquired stochastic process algebra model,the supply chain business process model is verified with Mobility Workbench.Finally,according to the operational semantics of stochastic process algebra,the continuous-time Markov chain,isomorphic with stochastic process algebra model,is built; and the system performance evaluation of transient status and stable status is respectively conducted in accordance with Markov transfer relations and the current state of system,obtaining the predicted performance value and average performance index value for a specific period of time.The simulation experiments show that the proposed method can accurately describe the stochastic behaviors of supply chain system and interactions among nodes,effectively verify the validity of the model,and objectively and exactly evaluate design of the supply chain. 展开更多
关键词 supply chain stochastic process algebra business process performance evaluation Markov chain
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基于视觉信息加工的草原公路行车安全性分析 被引量:3
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作者 王海晓 丁旭 +1 位作者 郭敏 吕贞 《重庆交通大学学报(自然科学版)》 CAS CSCD 北大核心 2024年第2期65-74,共10页
为探究草原公路各典型行车环境驾驶人视觉信息加工模式的差异性,从视觉层面解析草原公路行车安全,基于眼动仪开展草原公路实车实验,提取驾驶人在自由行驶、跟驰、对向来车、后车切入、路侧风险、交叉口6种真实行车环境下的眼动数据。首... 为探究草原公路各典型行车环境驾驶人视觉信息加工模式的差异性,从视觉层面解析草原公路行车安全,基于眼动仪开展草原公路实车实验,提取驾驶人在自由行驶、跟驰、对向来车、后车切入、路侧风险、交叉口6种真实行车环境下的眼动数据。首先,从视觉分配和视觉搜索两个维度构建评价指标体系,采用动态聚类法将驾驶人的视觉兴趣区域划分为5类,将信息熵与马尔可夫链平稳分布理论结合,构建视点分布信息熵量化驾驶人视觉注意分配的复杂度,并引入扫视幅度、扫视速度分析驾驶人对视觉信息的搜索过程。最后,构建基于CRITIC法的加权秩和比评价模型,对不同行车环境下驾驶人的视觉信息加工强度进行了综合评价。结果显示:驾驶人的视点分布在不同行车环境中存在差异,但当前车道始终是驾驶人获取视觉信息的主视区;各视觉评价指标随行车环境的变化呈显著的统计学差异;驾驶人遇到路侧风险时的视觉信息加工强度最大,行车安全性相对更低。 展开更多
关键词 交通运输工程 草原公路 视觉信息加工 马尔可夫链 信息熵 加权秩和比法
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一类纯跳种群系统的遍历性
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作者 张振中 陈业勤 +2 位作者 刘慧媛 李新平 赵馨 《华东师范大学学报(自然科学版)》 CAS CSCD 北大核心 2024年第2期1-13,共13页
为了刻画随机环境与重大突变因素对种群的影响,本文考虑一类由马氏链与纯跳稳定过程驱动互惠种群模型.首先,讨论了该种群模型具有全局正解性.其次,给出了该模型的遍历性的充分条件.最后,探究了该模型的正常返的条件.
关键词 a-稳定过程 马氏链 遍历性 正常返
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双循环背景下石化企业供应链韧性评价研究——基于AHP-BP方法 被引量:2
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作者 赵丽洲 张宁峰 《辽宁石油化工大学学报》 CAS 2024年第1期89-96,共8页
随着环境不确定性的提高,中国石化企业供应链稳定性需求日渐攀升,供应链韧性评价已经成为判断石化企业风险应对能力的重要手段。基于双循环背景,通过构建石化企业供应链韧性评估指标体系,利用层次分析法和BP神经网络,对石化企业供应链... 随着环境不确定性的提高,中国石化企业供应链稳定性需求日渐攀升,供应链韧性评价已经成为判断石化企业风险应对能力的重要手段。基于双循环背景,通过构建石化企业供应链韧性评估指标体系,利用层次分析法和BP神经网络,对石化企业供应链韧性强度进行评估,确定了供应链韧性水平。结果表明,各石化企业的供应链韧性强度存在较大差异,供应链韧性整体水平偏低。在研究结果的基础上,对韧性供应链锻造提出了切实可行的建议。 展开更多
关键词 石化企业 供应链韧性 层次分析法 BP神经网络算法
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粤港澳大湾区战略科技力量体系化布局:实践探索与突破路径
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作者 周振江 刘毅 +1 位作者 石义寿 段艳红 《科技创新发展战略研究》 2024年第2期46-55,共10页
战略科技力量是粤港澳大湾区建设具有全球影响力的国际科技创新中心、加快实现高水平科技自立自强的核心支撑。通过系统梳理粤港澳大湾区战略科技力量体系布局的发展现状和存在问题,发现当前其在数量规模、发展质量、空间分布、体制创... 战略科技力量是粤港澳大湾区建设具有全球影响力的国际科技创新中心、加快实现高水平科技自立自强的核心支撑。通过系统梳理粤港澳大湾区战略科技力量体系布局的发展现状和存在问题,发现当前其在数量规模、发展质量、空间分布、体制创新等方面仍存在不足之处。对此,从体系化布局粤港澳大湾区战略科技力量出发,提出聚焦全过程创新链,构建“筑根基、强攻关、搭平台、深融合、聚人才、建生态、重改革”的实施路径和策略。 展开更多
关键词 战略科技力量 体系化布局 全过程创新链 粤港澳大湾区
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数字孪生技术驱动产业链创新链融合发展的内涵、特征与实现路径 被引量:1
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作者 吴晓波 李王镕 +2 位作者 林福鑫 余点 浦贵阳 《科技管理研究》 CSSCI 2024年第4期110-118,共9页
产业链创新链(以下简称“双链”)融合发展是国内国际双循环格局下的必然要求,但链结构差异、网络碎片化、政策非协同等问题影响其流转效率及协同效果。在创新范式转变中,数字孪生技术展现出独特的动态过程映射和实时双向互动能力,为创... 产业链创新链(以下简称“双链”)融合发展是国内国际双循环格局下的必然要求,但链结构差异、网络碎片化、政策非协同等问题影响其流转效率及协同效果。在创新范式转变中,数字孪生技术展现出独特的动态过程映射和实时双向互动能力,为创新管理带来新思路。基于对“双链”内涵、融合现状与数字孪生技术特征、使能作用的系统性梳理,提出以数字孪生技术驱动“双链”融合发展的实现路径,在数字化标准表达与数据融合中实现全过程整合,跨越时间和空间的尺度实现互动与协同。具体来看,数字孪生技术在促进产业链创新链全过程整合与全时空交互中调节“双链”上下游各参与主体在运行机制、认知或目标上的结构性差异,控制产业网络和创新网络在发展过程中的碎片化趋向,转变产业政策与创新政策在设计布局、资源分配上的非协同问题,实现从单点优化范式到“双链”协同范式的重构与升级。然而,数字孪生技术仍存在技术实现、隐私安全、权责管理方面的不足,有待进一步探索和研究。 展开更多
关键词 数字孪生技术 产业链创新链融合 数字化 全过程整合 全时空交互 创新范式
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长碳链尼龙研究进展
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作者 胡双俊 《现代塑料加工应用》 CAS 北大核心 2024年第1期56-59,共4页
介绍了长碳链尼龙熔融缩聚法和溶液缩聚法工艺技术的研究状况,分析总结了相关研究思路和工艺路线。概述了近年来长碳链尼龙在增强、增韧及阻燃改性方面的研究进展。分析了长碳链尼龙的市场现况,涵盖了国内外主要的生产厂家,并对长碳链... 介绍了长碳链尼龙熔融缩聚法和溶液缩聚法工艺技术的研究状况,分析总结了相关研究思路和工艺路线。概述了近年来长碳链尼龙在增强、增韧及阻燃改性方面的研究进展。分析了长碳链尼龙的市场现况,涵盖了国内外主要的生产厂家,并对长碳链尼龙的改性研究未来趋势进行了展望。 展开更多
关键词 长碳链尼龙 聚合工艺 改性 研究进展
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基于AHP法的化工企业数字化供应链成熟度研究
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作者 夏露 顾彬 +1 位作者 刘玲 张彤 《物流科技》 2024年第7期121-126,共6页
人工智能、大数据等新兴技术推动着全球数字经济的发展,越来越多的制造业企业走上数字化转型的道路。数字化供应链将成为制造业企业发展的必然趋势,也是其数字化转型的关键。根据以往的研究成果,文章从企业战略管理、数字化技术应用、... 人工智能、大数据等新兴技术推动着全球数字经济的发展,越来越多的制造业企业走上数字化转型的道路。数字化供应链将成为制造业企业发展的必然趋势,也是其数字化转型的关键。根据以往的研究成果,文章从企业战略管理、数字化技术应用、数字化供应链业务流程和组织文化四个维度建立化工企业数字化供应链成熟度评估指标模型,通过层次分析法,得出各指标评分。并以A公司为例进行实证分析,验证模型的科学性,得出成熟度等级,为相关企业数字化供应链的发展提供帮助。 展开更多
关键词 数字化供应链 层次分析法 成熟度模型 化工企业
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“任务链+研学支架”教学模式的建构——新工科视角下“遥感数字图像处理”课教学改革
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作者 江振蓝 秦艳芳 +1 位作者 林木生 陈思明 《闽江学院学报》 2024年第1期101-108,共8页
目前,传统教学在内容的系统性、认知活动高阶性、价值引领实效性、促学效果等方面存在着较严重的问题,为此,“遥感数字图像处理”课程基于新工科教学理念,以遥感技术的综合应用情境重构课程体系,开启“任务链+研学支架”教学模式,加强... 目前,传统教学在内容的系统性、认知活动高阶性、价值引领实效性、促学效果等方面存在着较严重的问题,为此,“遥感数字图像处理”课程基于新工科教学理念,以遥感技术的综合应用情境重构课程体系,开启“任务链+研学支架”教学模式,加强培养学生遥感应用能力和创新能力,以遥感技术服务社会需求为育人路径。新模式的构建,实现了应用与研学互动、研学与研教并举、育人与育才同构的效果,对同类型新工科课程具有借鉴意义。 展开更多
关键词 “遥感数字图像处理”课程 “任务链+研学支架”教学模式 遥感技术 “新工科”教育理念
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绿色食品在乡村康养旅游中的重要性研究
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作者 鲁洋静 《食品安全导刊》 2024年第32期99-101,共3页
随着康养旅游需求的增长,绿色食品作为乡村康养旅游的核心要素,在满足游客康养需求、提升乡村旅游吸引力方面发挥了重要作用。本文对绿色食品的定义与分类进行探讨,并分析其在乡村康养旅游中的重要性和存在的问题,提出构建乡村绿色食品... 随着康养旅游需求的增长,绿色食品作为乡村康养旅游的核心要素,在满足游客康养需求、提升乡村旅游吸引力方面发挥了重要作用。本文对绿色食品的定义与分类进行探讨,并分析其在乡村康养旅游中的重要性和存在的问题,提出构建乡村绿色食品的全流程追溯系统、打造基于康养需求的定制化农产品供应链以及建立绿色食品与乡村旅游的协同品牌营销体系的策略建议。 展开更多
关键词 绿色食品 乡村康养旅游 全流程追溯 定制化供应链
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产品制造和装配过程认证DMAIC方法
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作者 汪泉发 《标准科学》 2024年第9期130-137,共8页
本文以扶梯梯级链制造和装配过程为例,提出了一套基于DMAIC的系统化和结构化过程认证方法,以减少过程变异,确保过程受控和有足够能力。主要方法是先通过流程图、SIPOC、PFMEA等分析,识别关键产品特性(KPC);然后针对各个KPC进行MSA、控... 本文以扶梯梯级链制造和装配过程为例,提出了一套基于DMAIC的系统化和结构化过程认证方法,以减少过程变异,确保过程受控和有足够能力。主要方法是先通过流程图、SIPOC、PFMEA等分析,识别关键产品特性(KPC);然后针对各个KPC进行MSA、控制图、过程能力分析,了解当前过程的能力水平;针对过程能力不达标的KPC,进行根源分析和改进,确保过程能力达到1.33以上;最后更新质量文件,制定过程控制和过程能力定期跟踪计划。 展开更多
关键词 扶梯梯级链 制造和装配过程 过程认证 DMAIC 质量管理
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海军某部官兵兴奋易感性与心理健康状态的关系:心理弹性和应对倾向的链式中介作用 被引量:1
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作者 张建国 毛晓飞 +4 位作者 陈艾彬 蔡文鹏 邓文曦 李自强 董薇 《海军军医大学学报》 CAS CSCD 北大核心 2024年第2期227-232,共6页
目的探索海军某部官兵心理健康状态与兴奋易感性、心理弹性和应对倾向的关系,阐明兴奋易感性影响心理健康的可能机制。方法采用心理健康自评问卷(SRQ-20)、心理弹性量表、简化版高敏感者量表10和简易应对方式问卷对1870名海军某部官兵... 目的探索海军某部官兵心理健康状态与兴奋易感性、心理弹性和应对倾向的关系,阐明兴奋易感性影响心理健康的可能机制。方法采用心理健康自评问卷(SRQ-20)、心理弹性量表、简化版高敏感者量表10和简易应对方式问卷对1870名海军某部官兵进行调查。采用Spearman相关法验证变量间的相关性,采用多元逐步回归分析评估兴奋易感性、心理弹性和应对倾向对心理健康状态的预测效果,通过构建结构方程模型探索心理弹性、应对倾向在兴奋易感性和心理健康状态之间的中介作用。结果官兵的SRQ-20得分为1(0,5)分,阳性检出率为21.2%(395/1865)。相关分析显示官兵心理健康状态与兴奋易感性呈正相关(P<0.01),与审美敏感性、心理弹性及应对倾向呈负相关(均P<0.01)。多元回归分析显示,兴奋易感性、心理弹性和应对倾向对心理健康状态有预测作用,心理弹性和应对倾向在兴奋易感性和心理健康状态之间起部分链式中介作用。结论海军某部官兵的兴奋易感性可以直接预测其心理健康状态,还可以通过心理弹性、应对倾向的独立及链式中介作用间接预测心理健康状态。 展开更多
关键词 心理健康 心理弹性 感觉加工敏感性 应对方式 链式中介效应
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湖南先进制造与现代服务深度融合的企业经管策略研究
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作者 陈兵 《机电产品开发与创新》 2024年第1期151-154,共4页
本文研究了制造业与服务业融合的湖南企业经营管理策略,提出了打造高效供应链的措施,例如供应商库存管理、联合库存和准时化采购;还从质量工程角度建议了质量提升的多种策略——质量循环曲线管理、车轮式管理、零瑕疵管理、全面质量管... 本文研究了制造业与服务业融合的湖南企业经营管理策略,提出了打造高效供应链的措施,例如供应商库存管理、联合库存和准时化采购;还从质量工程角度建议了质量提升的多种策略——质量循环曲线管理、车轮式管理、零瑕疵管理、全面质量管理、产品生命周期管理、6σ管理和过程能力指数管理;并探讨企业综合计划方略,倡导线性规划法的应用,研究了双作业中心的作业计划优化方法。 展开更多
关键词 供应链效率 过程能力控制 作业排序 两业融合
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噬菌肽SEYT4对食品链中常见致病菌的清除效果
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作者 杨甜 张月 +4 位作者 邹更 周洋 宋智勇 吴仁蔚 李锦铨 《华中农业大学学报》 CAS CSCD 北大核心 2024年第5期253-260,共8页
为探究噬菌肽在食品加工链中的应用前景,以1种含有37个氨基酸的新型噬菌肽SEYT4为研究对象,分析其结构特性,并选择食品加工链中常见的致病细菌,在不同温度、盐度及pH下进行杀菌试验,测定SEYT4的杀菌谱、杀菌效率及杀菌特性,利用C57BL/6... 为探究噬菌肽在食品加工链中的应用前景,以1种含有37个氨基酸的新型噬菌肽SEYT4为研究对象,分析其结构特性,并选择食品加工链中常见的致病细菌,在不同温度、盐度及pH下进行杀菌试验,测定SEYT4的杀菌谱、杀菌效率及杀菌特性,利用C57BL/6雌性小鼠评估SEYT4的安全性,并通过模拟被致病菌污染的叶菜和厨具,探究SEYT4清除食品链中致病菌的效果。结果显示:噬菌肽SEYT4是1个发夹状的双α-螺旋结构,分子质量约为4.5ku,理论等电点为9.69;能够清除大多数高毒性且耐药的革兰氏阳性和革兰氏阴性致病菌,杀菌率高达99.99%,在不同温度下均具有较高活性,且在中性和弱碱性环境下杀菌效果更好,但在高盐环境下会失活;对小鼠红细胞无溶血作用;在构建的人工污染香菜模型中能将叶片和洗涤液中的致病菌清除约90.00%,同时能清除食品接触面上99.00%以上的致病菌。结果表明,噬菌肽SEYT4是一种对致病菌有良好的杀菌效果且安全性高的新型抗菌肽,能够有效防控食品链中污染的致病菌,有望成为一种食品加工链中的实用消毒剂。 展开更多
关键词 食源性细菌 致病菌 噬菌肽 食品加工链 消毒剂
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