Background:Egg production is economically important in the meat-type chicken industry.To better understand the molecular genetic mechanism of egg production in meat-type chicken,genetic parameter estimation,genome-wid...Background:Egg production is economically important in the meat-type chicken industry.To better understand the molecular genetic mechanism of egg production in meat-type chicken,genetic parameter estimation,genome-wide association analyses combined with meta-analyses,Bayesian analyses,and selective sweep analyses were performed to screen single nucleotide polymorphisms(SNPs)and other genetic loci that were significantly associated with egg number traits in 11,279 chickens from seven material lines.Results:Yellow-feathered meat-type chickens laid 115 eggs at 43 weeks of age and white-feathered chickens laid 143 eggs at 60 weeks of age,with heritability ranging from 0.034–0.258.Based on meta-analyses and selective sweep analyses,one region(10.81–13.05 Mb)on chromosome Z was associated with egg number in all lines.Further analyses using the W2 line was also associated with the same region,and 29 SNPs were identified that significantly affected estimation of breeding value of egg numbers.The 29 SNPs were identified as having a significant effect on the egg number EBV in 3194 birds in line W2.There are 36 genes in the region,with glial cell derived neurotrophic factor,DAB adaptor protein 2,protein kinase AMP-activated catalytic subunit alpha 1,NAD kinase 2,mitochondrial,WD repeat domain 70,leukemia inhibitory factor receptor alpha,complement C6,and complement C7 identified as being potentially affecting to egg number.In addition,three SNPs(rs318154184,rs13769886,and rs313325646)associated with egg number were located on or near the prolactin receptor gene.Conclusion:Our study used genomic information from different chicken lines and populations to identify a genomic region(spanning 2.24 Mb)associated with egg number.Nine genes and 29 SNPs were identified as the most likely candidate genes and variations for egg production.These results contribute to the identification of candidate genes and variants for egg traits in poultry.展开更多
本研究旨在探索新疆褐牛产奶性状相关候选基因多态性与泌乳性状及体细胞评分(SCS)的相关性,探究新疆褐牛多态性位点的群体遗传特征。基于课题组前期DNA混池重测序的结果筛选17个SNPs突变信息,并采用基质辅助激光电离解吸飞行时间质谱法(...本研究旨在探索新疆褐牛产奶性状相关候选基因多态性与泌乳性状及体细胞评分(SCS)的相关性,探究新疆褐牛多态性位点的群体遗传特征。基于课题组前期DNA混池重测序的结果筛选17个SNPs突变信息,并采用基质辅助激光电离解吸飞行时间质谱法(Sequenom Mass Array Genotype)分型技术验证FASN、FSHR、HAL、GPIHBP1、NIBPL、C9、PDE9A、PTK2、STAT1、ZNF66、ZNF567、ZNF7、ZNF623和ZNF804A基因在新疆乌鲁木齐种牛场、新疆天山畜牧生物工程股份有限公司和新疆塔城地区种牛场共计262头新疆褐牛母牛群体中的遗传多态性,运用SAS 8.1软件GLM过程分析这14个基因多个SNP位点对新疆褐牛群体的遗传效应及其与305天产奶量、乳脂率、乳蛋白率、乳糖率、总固体和SCS的关联性。结果表明,17个SNPs均处于哈代-温伯格平衡状态(P>0.05),9个SNPs与新疆褐牛1个或多个泌乳性状达到显著(P<0.005)或极显著(P<0.001)关联,这些位点分别位于FASN、FSHR、HAL、ZNF66、ZNF7、ZNF804A、GPIHBP1和PDE9A基因上。提示这些基因可尝试作为新疆褐牛产奶性状分子标记的候选基因,为新疆褐牛产奶性状的分子标记辅助选育提供依据和参考。展开更多
基金the Guangdong Provincial Key Research and Development Program(2020B02022002)the Shandong Agricultural Seed Improvement Project(2020LZGC013)the Technological Innovation Project of Chinese Academy of Agricultural Sciences(CAAS-ZDRW202005)。
文摘Background:Egg production is economically important in the meat-type chicken industry.To better understand the molecular genetic mechanism of egg production in meat-type chicken,genetic parameter estimation,genome-wide association analyses combined with meta-analyses,Bayesian analyses,and selective sweep analyses were performed to screen single nucleotide polymorphisms(SNPs)and other genetic loci that were significantly associated with egg number traits in 11,279 chickens from seven material lines.Results:Yellow-feathered meat-type chickens laid 115 eggs at 43 weeks of age and white-feathered chickens laid 143 eggs at 60 weeks of age,with heritability ranging from 0.034–0.258.Based on meta-analyses and selective sweep analyses,one region(10.81–13.05 Mb)on chromosome Z was associated with egg number in all lines.Further analyses using the W2 line was also associated with the same region,and 29 SNPs were identified that significantly affected estimation of breeding value of egg numbers.The 29 SNPs were identified as having a significant effect on the egg number EBV in 3194 birds in line W2.There are 36 genes in the region,with glial cell derived neurotrophic factor,DAB adaptor protein 2,protein kinase AMP-activated catalytic subunit alpha 1,NAD kinase 2,mitochondrial,WD repeat domain 70,leukemia inhibitory factor receptor alpha,complement C6,and complement C7 identified as being potentially affecting to egg number.In addition,three SNPs(rs318154184,rs13769886,and rs313325646)associated with egg number were located on or near the prolactin receptor gene.Conclusion:Our study used genomic information from different chicken lines and populations to identify a genomic region(spanning 2.24 Mb)associated with egg number.Nine genes and 29 SNPs were identified as the most likely candidate genes and variations for egg production.These results contribute to the identification of candidate genes and variants for egg traits in poultry.
文摘本研究旨在探索新疆褐牛产奶性状相关候选基因多态性与泌乳性状及体细胞评分(SCS)的相关性,探究新疆褐牛多态性位点的群体遗传特征。基于课题组前期DNA混池重测序的结果筛选17个SNPs突变信息,并采用基质辅助激光电离解吸飞行时间质谱法(Sequenom Mass Array Genotype)分型技术验证FASN、FSHR、HAL、GPIHBP1、NIBPL、C9、PDE9A、PTK2、STAT1、ZNF66、ZNF567、ZNF7、ZNF623和ZNF804A基因在新疆乌鲁木齐种牛场、新疆天山畜牧生物工程股份有限公司和新疆塔城地区种牛场共计262头新疆褐牛母牛群体中的遗传多态性,运用SAS 8.1软件GLM过程分析这14个基因多个SNP位点对新疆褐牛群体的遗传效应及其与305天产奶量、乳脂率、乳蛋白率、乳糖率、总固体和SCS的关联性。结果表明,17个SNPs均处于哈代-温伯格平衡状态(P>0.05),9个SNPs与新疆褐牛1个或多个泌乳性状达到显著(P<0.005)或极显著(P<0.001)关联,这些位点分别位于FASN、FSHR、HAL、ZNF66、ZNF7、ZNF804A、GPIHBP1和PDE9A基因上。提示这些基因可尝试作为新疆褐牛产奶性状分子标记的候选基因,为新疆褐牛产奶性状的分子标记辅助选育提供依据和参考。
