Objective： The aim of the study was to observe the effect of total isoflavones from pueraria Iobata （TIP） on D2 dopamine receptor mRNA, preproenkephalin mRNA and prodynorphin mRNA expressions in Parkinson＇s disea...Objective： The aim of the study was to observe the effect of total isoflavones from pueraria Iobata （TIP） on D2 dopamine receptor mRNA, preproenkephalin mRNA and prodynorphin mRNA expressions in Parkinson＇s disease （PD） model cells induced by 1-methyl-4-phenylpyridinium ion （MPP^＋）. Methods： TIP was dissolved in 0.1 M NaOH and added to the culture medium at a final concentrations of 50 mg/L, 100 mg/L and 200 mg/L. Some cells （control） were exposed to 0.001 M NaOH. TIP was added to PC12 cells 30 min prior to the administration of MPP^＋. TIP and MPP^＋ remained in the culture medium for 96 h. D2 dopamine receptor mRNA, preproenkephalin mRNA and prodynorphin mRNA expressions were assayed by real-time quantitative reverse transcription-PCR. Results： The D2 dopamine receptor mRNA and preproenkephalin mRNA expressions were up-regulated in MPP^＋ group compared with the control group, and prodynorphin mRNA expression was down-regulated in that. The D2 dopamine receptor mRNA expression being down-regulated and prodynorphin mRNA expression being up-regulated in TIP group compared with the MPP^＋ group. And there was no effect of TIP on preproenkephalin gene expression in PC12 cells induced by MPP^＋. Conclusion： The results suggest that TIP down-regulates the D2 dopamine receptor mRNA expression, up-regulates prodynorphin mRNA expression and not affects preproenkephalin gene expression in PC12 cells induced by MPP^＋.展开更多
Objective To study the changes of prodynorphin （PDyn） gene expression and dopamine and cAMPregulated phosphoprotein of 32 kDa （DARPP-32） phosphorylation in rats with levodopa-induced dyskinesias （LID）, and to ex...Objective To study the changes of prodynorphin （PDyn） gene expression and dopamine and cAMPregulated phosphoprotein of 32 kDa （DARPP-32） phosphorylation in rats with levodopa-induced dyskinesias （LID）, and to explore the mechanism of over-activation in direct pathway mediated by dopamine D1 receptor. Methods Parkinson＇s disease （PD） rats were received levodopa （10 mg/kg, i.p.） for 28 d to get the LID rats. According to the behavior scale, LID rats were divided into mild （n=8） and severe （n=16） groups. On day 29, 8 rats in severe LID group were given an acute intraperitoneal injection of MK-801 （0.1 mg/kg） 15 min before levodopa treatment （MK-801 group, n=8）. The normal rats received same course and dosage of levodopa as the control group （n=8）. Hybridization in situ was used to measure the expression of PDyn mRNA in striatum. Protein and mRNA levels of total DARPP-32 and phospho-Thr-34 DARPP-32 level were measured by immunoblotting and RT-PCR, respectively. Results The levels of PDyn mRNA and phospho-Thr-34 DARPP-32 increased significantly in LID rats compared with control rats （P〈0.01）, and they also increased markedly in severe LID group compared with mild group （P〈0.01）. Conclusion Phospho-Thr-34 DARPP-32 level was increased in LID rats, which contributed to the over-activation of direct pathway mediated by dopamine D1 receptor.展开更多
目的探讨γ-氨基丁酸B受体B1亚单位（GA BA BR1）基因（G1465A）多态性及前强啡肽原（PDYN）启动子区基因多态性与汉族人群脑梗死继发癫痫发生之间的关系。方法选择郑州大学第五、第一附属医院神经内科自2010年7月至2011年8月住院及门...目的探讨γ-氨基丁酸B受体B1亚单位（GA BA BR1）基因（G1465A）多态性及前强啡肽原（PDYN）启动子区基因多态性与汉族人群脑梗死继发癫痫发生之间的关系。方法选择郑州大学第五、第一附属医院神经内科自2010年7月至2011年8月住院及门诊收治的67例脑梗死继发癫痫患者、同期收治的93例脑梗死无癫痫患者和104例体检正常者为研究对象．采集各组对象肘静脉血，柱层析法提取血细胞基因组DNA，聚合酶链式反应-限制性片段长度多态（PCR-RFLP）技术检测GA BA BRl基因（G1465A）多态性，聚合酶链式反应（PCR）技术检测PDYN启动子区基因多态性。结果3组研究对象酬鲋BRJ基因（Gl465A）都显示为G／G型，未发现A／G型和A／A型。脑梗死继发癫痫组中PDYN启动子区基因L／L型频率（76．1％）略高于脑梗死无癫痫组（73．2％）及正常对照组（73．1％），L型等位基因频率（85．8％）略高于脑梗死无癫痫组（85．5％）及正常对照组（84．6％），3组间基因型频率和等位基因频率比较差异均无统计学意义（P＞O．05）。结论GABABRJ基因（G1465A）及PDYN启动子区基因多态性与汉族人群脑梗死继发癫痫可能无明显关系。展开更多
基金Supported by the grants from the National Natural Science Foundation of China (No. 30873396)the National Science Foundation for Postdoctoral Scientists of China (No. 20080430140)+1 种基金Research Foundation of Education Bureau of Heilongjiang Province (No. 11511455)the Qiqihar Foundation for Development of Science and Technology, China(No. SF-08002)
文摘Objective： The aim of the study was to observe the effect of total isoflavones from pueraria Iobata （TIP） on D2 dopamine receptor mRNA, preproenkephalin mRNA and prodynorphin mRNA expressions in Parkinson＇s disease （PD） model cells induced by 1-methyl-4-phenylpyridinium ion （MPP^＋）. Methods： TIP was dissolved in 0.1 M NaOH and added to the culture medium at a final concentrations of 50 mg/L, 100 mg/L and 200 mg/L. Some cells （control） were exposed to 0.001 M NaOH. TIP was added to PC12 cells 30 min prior to the administration of MPP^＋. TIP and MPP^＋ remained in the culture medium for 96 h. D2 dopamine receptor mRNA, preproenkephalin mRNA and prodynorphin mRNA expressions were assayed by real-time quantitative reverse transcription-PCR. Results： The D2 dopamine receptor mRNA and preproenkephalin mRNA expressions were up-regulated in MPP^＋ group compared with the control group, and prodynorphin mRNA expression was down-regulated in that. The D2 dopamine receptor mRNA expression being down-regulated and prodynorphin mRNA expression being up-regulated in TIP group compared with the MPP^＋ group. And there was no effect of TIP on preproenkephalin gene expression in PC12 cells induced by MPP^＋. Conclusion： The results suggest that TIP down-regulates the D2 dopamine receptor mRNA expression, up-regulates prodynorphin mRNA expression and not affects preproenkephalin gene expression in PC12 cells induced by MPP^＋.
文摘Objective To study the changes of prodynorphin （PDyn） gene expression and dopamine and cAMPregulated phosphoprotein of 32 kDa （DARPP-32） phosphorylation in rats with levodopa-induced dyskinesias （LID）, and to explore the mechanism of over-activation in direct pathway mediated by dopamine D1 receptor. Methods Parkinson＇s disease （PD） rats were received levodopa （10 mg/kg, i.p.） for 28 d to get the LID rats. According to the behavior scale, LID rats were divided into mild （n=8） and severe （n=16） groups. On day 29, 8 rats in severe LID group were given an acute intraperitoneal injection of MK-801 （0.1 mg/kg） 15 min before levodopa treatment （MK-801 group, n=8）. The normal rats received same course and dosage of levodopa as the control group （n=8）. Hybridization in situ was used to measure the expression of PDyn mRNA in striatum. Protein and mRNA levels of total DARPP-32 and phospho-Thr-34 DARPP-32 level were measured by immunoblotting and RT-PCR, respectively. Results The levels of PDyn mRNA and phospho-Thr-34 DARPP-32 increased significantly in LID rats compared with control rats （P〈0.01）, and they also increased markedly in severe LID group compared with mild group （P〈0.01）. Conclusion Phospho-Thr-34 DARPP-32 level was increased in LID rats, which contributed to the over-activation of direct pathway mediated by dopamine D1 receptor.
文摘目的探讨γ-氨基丁酸B受体B1亚单位（GA BA BR1）基因（G1465A）多态性及前强啡肽原（PDYN）启动子区基因多态性与汉族人群脑梗死继发癫痫发生之间的关系。方法选择郑州大学第五、第一附属医院神经内科自2010年7月至2011年8月住院及门诊收治的67例脑梗死继发癫痫患者、同期收治的93例脑梗死无癫痫患者和104例体检正常者为研究对象．采集各组对象肘静脉血，柱层析法提取血细胞基因组DNA，聚合酶链式反应-限制性片段长度多态（PCR-RFLP）技术检测GA BA BRl基因（G1465A）多态性，聚合酶链式反应（PCR）技术检测PDYN启动子区基因多态性。结果3组研究对象酬鲋BRJ基因（Gl465A）都显示为G／G型，未发现A／G型和A／A型。脑梗死继发癫痫组中PDYN启动子区基因L／L型频率（76．1％）略高于脑梗死无癫痫组（73．2％）及正常对照组（73．1％），L型等位基因频率（85．8％）略高于脑梗死无癫痫组（85．5％）及正常对照组（84．6％），3组间基因型频率和等位基因频率比较差异均无统计学意义（P＞O．05）。结论GABABRJ基因（G1465A）及PDYN启动子区基因多态性与汉族人群脑梗死继发癫痫可能无明显关系。
