Objective To explore the migration of transplanted neural stem cells co-labeled with superparamagnetic iron oxide （SPIO） and bromodeoxyuridine （Brdu） using the 4.7T MR system and to study the cell differentiation ...Objective To explore the migration of transplanted neural stem cells co-labeled with superparamagnetic iron oxide （SPIO） and bromodeoxyuridine （Brdu） using the 4.7T MR system and to study the cell differentiation with immuno-histochemical method in ischemic rats. Methods Rat neural stem cells （NSCs） co-labelled with SPIO mediated by poly-L-lysine and bromodeoxyuridine （BrdU） were transplanted into the unaffected side of rat brain with middle cerebral artery occlusion （MCAO）. At weeks 1, 2, 3, 4, 5, and 6 after MCAO, migration of the labelled cells was monitored by MRI. At week 6 the rats were killed and their brain tissue was cut according to the migration site of transplanted ceils indicated by MRI and subjected to Prussian blue staining and immunohistochemical staining to observe the migration and differentiation of the transplanted NSCs. Results Three weeks after transplantation, the linear hypointensity area derived from the migration of labelled NSCs was observed by MRI in the corpus callosum adjacent to the injection site. Six weeks after the transplantation, the linear hypointensity area was moved toward the midline along the corpus callosum. MRI findings were confirmed by Prussian blue staining and immunohistochemical staining of the specimen at week 6 after the transplantation. Flourescence co-labelled immunohistochemical methods demonstrated that the transplanted NSCs could differentiate into astrocytes and neurons. Conclusion MRI can monitor the migration of SPIO-labelled NSCs after transplantation in a dynamical and non-invasive manner. NSCs transplanted into ischemic rats can differentiate into astrocytes and neurons during the process of migration.展开更多
特殊染色在神经系统疾病诊断中发挥着不可替代的作用。某些特殊染色方法可以标记神经元特有的结构,如Nissl染色标记尼氏体,用于观察神经元并判断其损伤程度;或应用于神经系统疾病的诊断,如在原发性脱髓鞘疾病的诊断中,Luxol Fast Blue...特殊染色在神经系统疾病诊断中发挥着不可替代的作用。某些特殊染色方法可以标记神经元特有的结构,如Nissl染色标记尼氏体,用于观察神经元并判断其损伤程度;或应用于神经系统疾病的诊断,如在原发性脱髓鞘疾病的诊断中,Luxol Fast Blue染色所显示的髓鞘脱失、Bodian染色所显示的相对保留的轴索、Holzer染色所显示的反应性增生的星形细胞等。病理科医师在日常神经病理诊断工作中如果能够正确、巧妙地应用特殊染色往往可以达到事半功倍的效果。展开更多
基金the National Natural Science Foundation of China, No. 30300093, 30570628, 30770751
文摘Objective To explore the migration of transplanted neural stem cells co-labeled with superparamagnetic iron oxide （SPIO） and bromodeoxyuridine （Brdu） using the 4.7T MR system and to study the cell differentiation with immuno-histochemical method in ischemic rats. Methods Rat neural stem cells （NSCs） co-labelled with SPIO mediated by poly-L-lysine and bromodeoxyuridine （BrdU） were transplanted into the unaffected side of rat brain with middle cerebral artery occlusion （MCAO）. At weeks 1, 2, 3, 4, 5, and 6 after MCAO, migration of the labelled cells was monitored by MRI. At week 6 the rats were killed and their brain tissue was cut according to the migration site of transplanted ceils indicated by MRI and subjected to Prussian blue staining and immunohistochemical staining to observe the migration and differentiation of the transplanted NSCs. Results Three weeks after transplantation, the linear hypointensity area derived from the migration of labelled NSCs was observed by MRI in the corpus callosum adjacent to the injection site. Six weeks after the transplantation, the linear hypointensity area was moved toward the midline along the corpus callosum. MRI findings were confirmed by Prussian blue staining and immunohistochemical staining of the specimen at week 6 after the transplantation. Flourescence co-labelled immunohistochemical methods demonstrated that the transplanted NSCs could differentiate into astrocytes and neurons. Conclusion MRI can monitor the migration of SPIO-labelled NSCs after transplantation in a dynamical and non-invasive manner. NSCs transplanted into ischemic rats can differentiate into astrocytes and neurons during the process of migration.
文摘特殊染色在神经系统疾病诊断中发挥着不可替代的作用。某些特殊染色方法可以标记神经元特有的结构,如Nissl染色标记尼氏体,用于观察神经元并判断其损伤程度;或应用于神经系统疾病的诊断,如在原发性脱髓鞘疾病的诊断中,Luxol Fast Blue染色所显示的髓鞘脱失、Bodian染色所显示的相对保留的轴索、Holzer染色所显示的反应性增生的星形细胞等。病理科医师在日常神经病理诊断工作中如果能够正确、巧妙地应用特殊染色往往可以达到事半功倍的效果。
