Beginning with a 5D homogeneous universe [1], we have provided a plausible explanation of the self-rotation phenomenon of stellar objects previously with illustration of large number of star samples [2], via a 5D-4D p...Beginning with a 5D homogeneous universe [1], we have provided a plausible explanation of the self-rotation phenomenon of stellar objects previously with illustration of large number of star samples [2], via a 5D-4D projection. The origin of such rotation is the balance of the angular momenta of stars and that of positive and negative charged e-trino pairs, within a 3D ⊗1D?void of the stellar object, the existence of which is based on conservation/parity laws in physics if one starts with homogeneous 5D universe. While the in-phase e-trino pairs are proposed to be responsible for the generation of angular momentum, the anti-phase but oppositely charge pairs necessarily produce currents. In the 5D to 4D projection, one space variable in the 5D manifold was compacted to zero in most other 5D theories (including theories of Kaluza-Klein and Einstein [3] [4]). We have demonstrated, using the Fermat’s Last Theorem [5], that for validity of gauge invariance at the 4D-5D boundary, the 4th space variable in the 5D manifold is mapped into two current rings at both magnetic poles as required by Perelman entropy mapping;these loops are the origin of the dipolar magnetic field. One conclusion we draw is that there is no gravitational singularity, and hence no black holes in the universe, a result strongly supported by the recent discovery of many stars with masses well greater than 100 solar mass [6] [7] [8], without trace of phenomena observed (such as strong gamma and X ray emissions), which are supposed to be associated with black holes. We analyze the properties of such loop currents on the 4D-5D boundary, where Maxwell equations are valid. We derive explicit expressions for the dipolar fields over the whole temperature range. We then compare our prediction with measured surface magnetic fields of many stars. Since there is coupling in distribution between the in-phase and anti-phase pairs of e-trinos, the generated mag-netic field is directly related to the angular momentum, leading to the result that the magnetic field can be expressible in terms of only the mechanical variables (mass M, radius R, rotation period P)of a star, as if Maxwell equations are “hidden”. An explanation for the occurrence of this “un-expected result” is provided in Section (7.6). Therefore we provide satisfactory answers to a number of “mysteries” of magnetism in astrophysics such as the “Magnetic Bode’s Relation/Law” [9] and the experimental finding that B-P graph in the log-log plot is linear. Moreover, we have developed a new method for studying the relations among the data (M, R, P) during stellar evolution. Ten groups of stellar objects, effectively over 2000 samples are used in various parts of the analysis. We also explain the emergence of huge magnetic field in very old stars like White Dwarfs in terms of formation of 2D Semion state on stellar surface and release of magnetic flux as magnetic storms upon changing the 2D state back to 3D structure. Moreover, we provide an explanation, on the ground of the 5D theory, for the detection of extremely weak fields in Venus and Mars and the asymmetric distribution of magnetic field on the Martian surface. We predict the equatorial fields B of the newly discovered Trappist-1 star and the 6 nearest planets. The log B?−?log P graph for the 6 planets is linear and they satisfy the Magnetic Bode’s relation. Based on the above analysis, we have discovered several new laws of stellar magnetism, which are summarized in Section (7.6).展开更多
Bangladesh is a densely populated country than many other countries of the world. The population growth is termed as alarming, however, knowledge of growth in the years to come would be useful in planning for the deve...Bangladesh is a densely populated country than many other countries of the world. The population growth is termed as alarming, however, knowledge of growth in the years to come would be useful in planning for the development of the country. This article is based on the projection of future population growth of the country. The available actual population census data during 1991-2011 of Bangladesh was applied to the application of a non-linear, non-autonomous ordinary differential equation familiar as Verhulst logistic population model with the maximum environmental capability of Bangladesh. Bangladesh will reach its carrying capacity of 245.09 million population in the next 56 years i.e. the year 2067 and then it decreases as S-shaped curve. The article has provided a focus on the changing trends of the growth of the population of Bangladesh.展开更多
In a dynamic CT, the acquired projections are corrupted due to strong dynamic nature of the object, for example: lungs, heart etc. In this paper, we present fan-beam reconstruction algorithm without position-dependent...In a dynamic CT, the acquired projections are corrupted due to strong dynamic nature of the object, for example: lungs, heart etc. In this paper, we present fan-beam reconstruction algorithm without position-dependent backprojection weight which compensates for the time-dependent translational, uniform scaling and rotational deformations occurring in the object of interest during the data acquisition process. We shall also compare the computational cost of the proposed reconstruction algorithm with the existing one which has position-dependent weight. To accomplish the objective listed above, we first formulate admissibility conditions on deformations that is required to exactly reconstruct the object from acquired sequential deformed projections and then derive the reconstruction algorithm to compensate the above listed deformations satisfying the admissibility conditions. For this, 2-D time-dependent deformation model is incorporated in the fan-beam FBP reconstruction algorithm with no backprojection weight, assuming the motion parameters being known. Finally the proposed reconstruction algorithm is evaluated with the motion corrupted projection data simulated on the computer.展开更多
Modern computer techniques have been in use for several years to generate three-dimensional visualizations of human anatomy. Very good 3-D computer models of the human body are now available and used routinely in anat...Modern computer techniques have been in use for several years to generate three-dimensional visualizations of human anatomy. Very good 3-D computer models of the human body are now available and used routinely in anatomy instruction. These techniques are subsumed under the heading “virtual anatomy” to distinguish them from the conventional study of anatomy entailing cadavers and anatomy textbooks. Moreover, other imaging procedures (X-ray, angiography, CT and MR) are also used in virtual anatomy instruction. A recently introduced three-dimensional post-processing technique named Cinematic Rendering now makes it possible to use the output of routine CT and MR examinations as the basis for highly photo-realistic 3-D depictions of human anatomy. We have installed Cinematic Rendering (enabled for stereoscopy) in a high-definition 8K 3-D projection space that accommodates an audience of 150. The space’s projection surface measures 16 × 9 meters;images can be projected on both the front wall and the floor. A game controller can be used to operate Cinematic Rendering software so that it can generate interactive real-time depictions of human anatomy on the basis of CT and MR data sets. This prototype installation was implemented without technical problems;in day-to-day, real-world use over a period of 22 months, there were no impairments of service due to software crashes or other technical problems. We are already employing this installation routinely for educational offerings open to the public, courses for students in the health professions, and (continuing) professional education units for medical interns, residents and specialists—in, so to speak, the dissecting theater of the future.展开更多
Glutamatergic projection neurons generate sophisticated excitatory circuits to integrate and transmit information among different cortical areas,and between the neocortex and other regions of the brain and spinal cord...Glutamatergic projection neurons generate sophisticated excitatory circuits to integrate and transmit information among different cortical areas,and between the neocortex and other regions of the brain and spinal cord.Appropriate development of cortical projection neurons is regulated by certain essential events such as neural fate determination,proliferation,specification,differentiation,migration,survival,axonogenesis,and synaptogenesis.These processes are precisely regulated in a tempo-spatial manner by intrinsic factors,extrinsic signals,and neural activities.The generation of correct subtypes and precise connections of projection neurons is imperative not only to support the basic cortical functions(such as sensory information integration,motor coordination,and cognition)but also to prevent the onset and progression of neurodevelopmental disorders(such as intellectual disability,autism spectrum disorders,anxiety,and depression).This review mainly focuses on the recent progress of transcriptional regulations on the development and diversity of neocortical projection neurons and the clinical relevance of the failure of transcriptional modulations.展开更多
A transparent display simultaneously enables visualization of the images displayed on it as well as the view behind it,and therefore can be applied to,for instance,augmented reality(AR),virtual reality(VR),and head up...A transparent display simultaneously enables visualization of the images displayed on it as well as the view behind it,and therefore can be applied to,for instance,augmented reality(AR),virtual reality(VR),and head up display(HUD).Many solutions have been proposed for this purpose.Recently,the idea of frequency-selective scattering of red,green and blue light while transmitting visible light of other colours to achieve transparent projection display has been proposed,by taking advantage of metallic nanoparticle’s localized surface plasmon resonance(LSPR).In this article,a review of the recent progress of frequency-selective scattering of red,green and blue light that are based on metallic nanoparticle’s LSPR is presented.A discussion of method for choosing appropriate metal(s)is first given,followed by the definition of a figure of merit used to quantify the performance of a designed nanoparticle structure.Selective scattering of various nanostructures,including sphere-shaped nanoparticles,ellipsoidal nanoparticles,super-sphere core-shell nanoparticles,metallic nanocubes,and metallic nanoparticles combined with gain materials,are discussed in detail.Each nanostructure has its own advantages and disadvantages,but the combination of the metallic nanoparticle with gain materials is a more promising way since it has the potential to generate ultra-sharp scattering peaks(i.e.,high frequency-selectivity).展开更多
TiO2 particles coated with polystyrene which were prepared via in situ polymerization and oil green dye were dispersed in tetrachloroethylene and xylene, the mixture came to be electrophoretic ink and was encapsulated...TiO2 particles coated with polystyrene which were prepared via in situ polymerization and oil green dye were dispersed in tetrachloroethylene and xylene, the mixture came to be electrophoretic ink and was encapsulated in to microcapsules by complex coacervation from gelatin and a hydrolyzed copolymer of styrene and maleic anhydride（SMA）. It was demonstrated that the membranes of the microcapsules were formed from nano sized coacervate droplets resulting from gelation and hydrolyzed SMA, which leads to a compact membrane structure. Microcapsules were characterized in terms of microstructure, morphologies by scanning electron microscopy（SEM）. Electrophoretic display prototype was prepared by coating electrophoretic ink microcapsules slurry on ITO glass with nearly single layer and sealed by UV curable adhesives. The characters “Zheda” in Chinese was firstly displayed at a low volt 9 V D.C..展开更多
Social dysfunction is a risk factor for several neuropsychiatric illnesses.Previous studies have shown that the lateral septum(LS)-related pathway plays a critical role in mediating social behaviors.Howeve r,the role ...Social dysfunction is a risk factor for several neuropsychiatric illnesses.Previous studies have shown that the lateral septum(LS)-related pathway plays a critical role in mediating social behaviors.Howeve r,the role of the connections between the LS and its downstream brain regions in social behavio rs remains unclea r.In this study,we conducted a three-chamber test using electrophysiological and chemogenetic approaches in mice to determine how LS projections to ventral CA1(vCA1)influence sociability.Our res ults showed that gamma-aminobutyric acid(GABA)-e rgic neuro ns were activated following social experience,and that social behavio rs were enhanced by chemogenetic modulation of these neurons.Moreover,LS GABAergic neurons extended their functional neural connections via vCA1 glutamatergic pyramidal neurons,and regulating LSGABA→vCA1Gluneural projections affected social behaviors,which were impeded by suppressing LSprojecting vCA1 neuronal activity or inhibiting GABAAreceptors in vCA1.These findings support the hypothesis that LS inputs to the vCA1 can control social prefe rences and social novelty behaviors.These findings provide new insights rega rding the neural circuits that regulate sociability.展开更多
The crystal structure of the title complex, [Cu(C23H26N2)Cl2]2·2CH2Cl2, has been determined by single-crystal, X-ray diffraction techniques. The compound crystallizes as dark-green prisms in space group P21/c of ...The crystal structure of the title complex, [Cu(C23H26N2)Cl2]2·2CH2Cl2, has been determined by single-crystal, X-ray diffraction techniques. The compound crystallizes as dark-green prisms in space group P21/c of the monoclinic system, with Z=4 and unit cell dimensions a=1.710 9(7) nm, b=2.395 2(11) nm, c=1.348 9(6) nm and β=110.651(8)°. The complex consists of two similar but crystallographically independent dimers, of which Cu(Ⅱ) centers display five-coordinated trigonal-bipyramidal geometry with Cu-Cu separations in two centrosymmetric dimers are 0.345 1 and 0.347 8 nm, respectively, and in each dimer the two copper centers share a common edge formed by two bridging chloride ions, each being equatorial. Solvent molecules of CH2Cl2 are packed together in the crystal lattice. CCDC: 253299.展开更多
Oxidative stress contributes to the pathogenesis of neurodegenerative diseases.With the aim to find reagents that reduce oxidative stress,a phage display library was screened for peptides mimicking a2,6-sialyllactose(...Oxidative stress contributes to the pathogenesis of neurodegenerative diseases.With the aim to find reagents that reduce oxidative stress,a phage display library was screened for peptides mimicking a2,6-sialyllactose(6'-SL),which is known to beneficially influence neural functions.Using Sambucus nigra lectin,which specifically binds to 6'-SL,we screened a phage display library and found a peptide comprising identical sequences of 12 amino acids.Mimetic peptide,reverse peptide and scrambled peptide were tested for inhibition of 6'-SL binding to the lectin.Indeed,lectin binding to 6'-SL was inhibited by the most frequently identified mimetic peptide,but not by the reverse or scrambled peptides,showing that this peptide mimics 6'-SL.Functionally,mimetic peptide,but not the reverse or scrambled peptides,increased viability and expression of neural cell adhesion molecule L1 in SK-N-SH human neuroblastoma cells,and promoted survival and neurite outgrowth of cultured mouse cerebellar granule neurons challenged by H_20_2-induced oxidative stress.The combined results indicate that the 6'-SL mimetic peptide promotes neuronal survival and neuritogenesis,thus raising hopes for the treatment of neurodegenerative diseases.This study was approved by the Medical Ethics Committee of Shantou University Medical College,China(approval No.SUMC 2014-004)on February 20,2014.展开更多
The accessory olfactory bulb(AOB), located at the posterior dorsal aspect of the main olfactory bulb(MOB), is the first brain relay of the accessory olfactory system(AOS), which can parallelly detect and process volat...The accessory olfactory bulb(AOB), located at the posterior dorsal aspect of the main olfactory bulb(MOB), is the first brain relay of the accessory olfactory system(AOS), which can parallelly detect and process volatile and nonvolatile social chemosignals and mediate different sexual and social behaviors with the main olfactory system(MOS). However, due to its anatomical location and absence of specific markers, there is a lack of research on the internal and external neural circuits of the AOB. This issue was addressed by singlecolor labeling and fluorescent double labeling using retrograde rAAVs injected into the bed nucleus of the stria terminalis(BST), anterior cortical amygdalar area(ACo), medial amygdaloid nucleus(MeA), and posteromedial cortical amygdaloid area(PMCo) in mice. We demonstrated the effectiveness of this AOB projection neuron labeling method and showed that the mitral cells of the AOB exhibited efferent projection dispersion characteristics similar to those of the MOB. Moreover, there were significant differences in the number of neurons projected to different brain regions, which indicated that each mitral cell in the AOB could project to a different number of neurons in different cortices. These results provide a circuitry basis to help understand the mechanism by which pheromone information is encoded and decoded in the AOS.展开更多
文摘Beginning with a 5D homogeneous universe [1], we have provided a plausible explanation of the self-rotation phenomenon of stellar objects previously with illustration of large number of star samples [2], via a 5D-4D projection. The origin of such rotation is the balance of the angular momenta of stars and that of positive and negative charged e-trino pairs, within a 3D ⊗1D?void of the stellar object, the existence of which is based on conservation/parity laws in physics if one starts with homogeneous 5D universe. While the in-phase e-trino pairs are proposed to be responsible for the generation of angular momentum, the anti-phase but oppositely charge pairs necessarily produce currents. In the 5D to 4D projection, one space variable in the 5D manifold was compacted to zero in most other 5D theories (including theories of Kaluza-Klein and Einstein [3] [4]). We have demonstrated, using the Fermat’s Last Theorem [5], that for validity of gauge invariance at the 4D-5D boundary, the 4th space variable in the 5D manifold is mapped into two current rings at both magnetic poles as required by Perelman entropy mapping;these loops are the origin of the dipolar magnetic field. One conclusion we draw is that there is no gravitational singularity, and hence no black holes in the universe, a result strongly supported by the recent discovery of many stars with masses well greater than 100 solar mass [6] [7] [8], without trace of phenomena observed (such as strong gamma and X ray emissions), which are supposed to be associated with black holes. We analyze the properties of such loop currents on the 4D-5D boundary, where Maxwell equations are valid. We derive explicit expressions for the dipolar fields over the whole temperature range. We then compare our prediction with measured surface magnetic fields of many stars. Since there is coupling in distribution between the in-phase and anti-phase pairs of e-trinos, the generated mag-netic field is directly related to the angular momentum, leading to the result that the magnetic field can be expressible in terms of only the mechanical variables (mass M, radius R, rotation period P)of a star, as if Maxwell equations are “hidden”. An explanation for the occurrence of this “un-expected result” is provided in Section (7.6). Therefore we provide satisfactory answers to a number of “mysteries” of magnetism in astrophysics such as the “Magnetic Bode’s Relation/Law” [9] and the experimental finding that B-P graph in the log-log plot is linear. Moreover, we have developed a new method for studying the relations among the data (M, R, P) during stellar evolution. Ten groups of stellar objects, effectively over 2000 samples are used in various parts of the analysis. We also explain the emergence of huge magnetic field in very old stars like White Dwarfs in terms of formation of 2D Semion state on stellar surface and release of magnetic flux as magnetic storms upon changing the 2D state back to 3D structure. Moreover, we provide an explanation, on the ground of the 5D theory, for the detection of extremely weak fields in Venus and Mars and the asymmetric distribution of magnetic field on the Martian surface. We predict the equatorial fields B of the newly discovered Trappist-1 star and the 6 nearest planets. The log B?−?log P graph for the 6 planets is linear and they satisfy the Magnetic Bode’s relation. Based on the above analysis, we have discovered several new laws of stellar magnetism, which are summarized in Section (7.6).
文摘Bangladesh is a densely populated country than many other countries of the world. The population growth is termed as alarming, however, knowledge of growth in the years to come would be useful in planning for the development of the country. This article is based on the projection of future population growth of the country. The available actual population census data during 1991-2011 of Bangladesh was applied to the application of a non-linear, non-autonomous ordinary differential equation familiar as Verhulst logistic population model with the maximum environmental capability of Bangladesh. Bangladesh will reach its carrying capacity of 245.09 million population in the next 56 years i.e. the year 2067 and then it decreases as S-shaped curve. The article has provided a focus on the changing trends of the growth of the population of Bangladesh.
文摘In a dynamic CT, the acquired projections are corrupted due to strong dynamic nature of the object, for example: lungs, heart etc. In this paper, we present fan-beam reconstruction algorithm without position-dependent backprojection weight which compensates for the time-dependent translational, uniform scaling and rotational deformations occurring in the object of interest during the data acquisition process. We shall also compare the computational cost of the proposed reconstruction algorithm with the existing one which has position-dependent weight. To accomplish the objective listed above, we first formulate admissibility conditions on deformations that is required to exactly reconstruct the object from acquired sequential deformed projections and then derive the reconstruction algorithm to compensate the above listed deformations satisfying the admissibility conditions. For this, 2-D time-dependent deformation model is incorporated in the fan-beam FBP reconstruction algorithm with no backprojection weight, assuming the motion parameters being known. Finally the proposed reconstruction algorithm is evaluated with the motion corrupted projection data simulated on the computer.
文摘Modern computer techniques have been in use for several years to generate three-dimensional visualizations of human anatomy. Very good 3-D computer models of the human body are now available and used routinely in anatomy instruction. These techniques are subsumed under the heading “virtual anatomy” to distinguish them from the conventional study of anatomy entailing cadavers and anatomy textbooks. Moreover, other imaging procedures (X-ray, angiography, CT and MR) are also used in virtual anatomy instruction. A recently introduced three-dimensional post-processing technique named Cinematic Rendering now makes it possible to use the output of routine CT and MR examinations as the basis for highly photo-realistic 3-D depictions of human anatomy. We have installed Cinematic Rendering (enabled for stereoscopy) in a high-definition 8K 3-D projection space that accommodates an audience of 150. The space’s projection surface measures 16 × 9 meters;images can be projected on both the front wall and the floor. A game controller can be used to operate Cinematic Rendering software so that it can generate interactive real-time depictions of human anatomy on the basis of CT and MR data sets. This prototype installation was implemented without technical problems;in day-to-day, real-world use over a period of 22 months, there were no impairments of service due to software crashes or other technical problems. We are already employing this installation routinely for educational offerings open to the public, courses for students in the health professions, and (continuing) professional education units for medical interns, residents and specialists—in, so to speak, the dissecting theater of the future.
基金supported by Guangdong Provincial Basic and Applied Basic Research Fund,No.2021A1515011299(to KT)。
文摘Glutamatergic projection neurons generate sophisticated excitatory circuits to integrate and transmit information among different cortical areas,and between the neocortex and other regions of the brain and spinal cord.Appropriate development of cortical projection neurons is regulated by certain essential events such as neural fate determination,proliferation,specification,differentiation,migration,survival,axonogenesis,and synaptogenesis.These processes are precisely regulated in a tempo-spatial manner by intrinsic factors,extrinsic signals,and neural activities.The generation of correct subtypes and precise connections of projection neurons is imperative not only to support the basic cortical functions(such as sensory information integration,motor coordination,and cognition)but also to prevent the onset and progression of neurodevelopmental disorders(such as intellectual disability,autism spectrum disorders,anxiety,and depression).This review mainly focuses on the recent progress of transcriptional regulations on the development and diversity of neocortical projection neurons and the clinical relevance of the failure of transcriptional modulations.
文摘A transparent display simultaneously enables visualization of the images displayed on it as well as the view behind it,and therefore can be applied to,for instance,augmented reality(AR),virtual reality(VR),and head up display(HUD).Many solutions have been proposed for this purpose.Recently,the idea of frequency-selective scattering of red,green and blue light while transmitting visible light of other colours to achieve transparent projection display has been proposed,by taking advantage of metallic nanoparticle’s localized surface plasmon resonance(LSPR).In this article,a review of the recent progress of frequency-selective scattering of red,green and blue light that are based on metallic nanoparticle’s LSPR is presented.A discussion of method for choosing appropriate metal(s)is first given,followed by the definition of a figure of merit used to quantify the performance of a designed nanoparticle structure.Selective scattering of various nanostructures,including sphere-shaped nanoparticles,ellipsoidal nanoparticles,super-sphere core-shell nanoparticles,metallic nanocubes,and metallic nanoparticles combined with gain materials,are discussed in detail.Each nanostructure has its own advantages and disadvantages,but the combination of the metallic nanoparticle with gain materials is a more promising way since it has the potential to generate ultra-sharp scattering peaks(i.e.,high frequency-selectivity).
文摘TiO2 particles coated with polystyrene which were prepared via in situ polymerization and oil green dye were dispersed in tetrachloroethylene and xylene, the mixture came to be electrophoretic ink and was encapsulated in to microcapsules by complex coacervation from gelatin and a hydrolyzed copolymer of styrene and maleic anhydride（SMA）. It was demonstrated that the membranes of the microcapsules were formed from nano sized coacervate droplets resulting from gelation and hydrolyzed SMA, which leads to a compact membrane structure. Microcapsules were characterized in terms of microstructure, morphologies by scanning electron microscopy（SEM）. Electrophoretic display prototype was prepared by coating electrophoretic ink microcapsules slurry on ITO glass with nearly single layer and sealed by UV curable adhesives. The characters “Zheda” in Chinese was firstly displayed at a low volt 9 V D.C..
基金supported by the National Natural Science Foundation of China,No.82171521(to CL)the Special Funds ofTaishan Scholars Project of Shandong Province,No.tsqn202211368(to CL)+2 种基金the Natural Science Foundation of Shandong Province,Nos.ZR2022YQ65(to CL),ZR2021MH073(to CL),ZR2019PH109(to WW)the Projects of Medical and Health Technology Development Program in Shandong Province,China,Nos.202003090720(to DZ),202003070728(to JL),2019 WS329(to DW)the Scientific Research Foundation of Binzhou Medical University,No.BY2018KJ21(to DW)。
文摘Social dysfunction is a risk factor for several neuropsychiatric illnesses.Previous studies have shown that the lateral septum(LS)-related pathway plays a critical role in mediating social behaviors.Howeve r,the role of the connections between the LS and its downstream brain regions in social behavio rs remains unclea r.In this study,we conducted a three-chamber test using electrophysiological and chemogenetic approaches in mice to determine how LS projections to ventral CA1(vCA1)influence sociability.Our res ults showed that gamma-aminobutyric acid(GABA)-e rgic neuro ns were activated following social experience,and that social behavio rs were enhanced by chemogenetic modulation of these neurons.Moreover,LS GABAergic neurons extended their functional neural connections via vCA1 glutamatergic pyramidal neurons,and regulating LSGABA→vCA1Gluneural projections affected social behaviors,which were impeded by suppressing LSprojecting vCA1 neuronal activity or inhibiting GABAAreceptors in vCA1.These findings support the hypothesis that LS inputs to the vCA1 can control social prefe rences and social novelty behaviors.These findings provide new insights rega rding the neural circuits that regulate sociability.
文摘The crystal structure of the title complex, [Cu(C23H26N2)Cl2]2·2CH2Cl2, has been determined by single-crystal, X-ray diffraction techniques. The compound crystallizes as dark-green prisms in space group P21/c of the monoclinic system, with Z=4 and unit cell dimensions a=1.710 9(7) nm, b=2.395 2(11) nm, c=1.348 9(6) nm and β=110.651(8)°. The complex consists of two similar but crystallographically independent dimers, of which Cu(Ⅱ) centers display five-coordinated trigonal-bipyramidal geometry with Cu-Cu separations in two centrosymmetric dimers are 0.345 1 and 0.347 8 nm, respectively, and in each dimer the two copper centers share a common edge formed by two bridging chloride ions, each being equatorial. Solvent molecules of CH2Cl2 are packed together in the crystal lattice. CCDC: 253299.
基金supported by the National Natural Science Foundation of China,No.81471279 and No.81171138(to WJZ)Talent Support Grant from Shantou University Medical College,China,No.2501220118(to WJZ)the Li Kashing Foundation,No.LD030302(to MS)
文摘Oxidative stress contributes to the pathogenesis of neurodegenerative diseases.With the aim to find reagents that reduce oxidative stress,a phage display library was screened for peptides mimicking a2,6-sialyllactose(6'-SL),which is known to beneficially influence neural functions.Using Sambucus nigra lectin,which specifically binds to 6'-SL,we screened a phage display library and found a peptide comprising identical sequences of 12 amino acids.Mimetic peptide,reverse peptide and scrambled peptide were tested for inhibition of 6'-SL binding to the lectin.Indeed,lectin binding to 6'-SL was inhibited by the most frequently identified mimetic peptide,but not by the reverse or scrambled peptides,showing that this peptide mimics 6'-SL.Functionally,mimetic peptide,but not the reverse or scrambled peptides,increased viability and expression of neural cell adhesion molecule L1 in SK-N-SH human neuroblastoma cells,and promoted survival and neurite outgrowth of cultured mouse cerebellar granule neurons challenged by H_20_2-induced oxidative stress.The combined results indicate that the 6'-SL mimetic peptide promotes neuronal survival and neuritogenesis,thus raising hopes for the treatment of neurodegenerative diseases.This study was approved by the Medical Ethics Committee of Shantou University Medical College,China(approval No.SUMC 2014-004)on February 20,2014.
基金supported by the National Natural Science Foundation of China(31400946,31771156,91632303/H09,91732304 and 31830035)Strategic Priority Research Program of the Chinese Academy of Sciences(XDB32030200)。
文摘The accessory olfactory bulb(AOB), located at the posterior dorsal aspect of the main olfactory bulb(MOB), is the first brain relay of the accessory olfactory system(AOS), which can parallelly detect and process volatile and nonvolatile social chemosignals and mediate different sexual and social behaviors with the main olfactory system(MOS). However, due to its anatomical location and absence of specific markers, there is a lack of research on the internal and external neural circuits of the AOB. This issue was addressed by singlecolor labeling and fluorescent double labeling using retrograde rAAVs injected into the bed nucleus of the stria terminalis(BST), anterior cortical amygdalar area(ACo), medial amygdaloid nucleus(MeA), and posteromedial cortical amygdaloid area(PMCo) in mice. We demonstrated the effectiveness of this AOB projection neuron labeling method and showed that the mitral cells of the AOB exhibited efferent projection dispersion characteristics similar to those of the MOB. Moreover, there were significant differences in the number of neurons projected to different brain regions, which indicated that each mitral cell in the AOB could project to a different number of neurons in different cortices. These results provide a circuitry basis to help understand the mechanism by which pheromone information is encoded and decoded in the AOS.
