To test the antigenic activity of M protein (Mc protein) in the inner membrane of SARS-CoV, SARS-CoV Mc protein's bases locating inside the membrane were cloned, the His-fusion protein was expressed in E. coli and...To test the antigenic activity of M protein (Mc protein) in the inner membrane of SARS-CoV, SARS-CoV Mc protein's bases locating inside the membrane were cloned, the His-fusion protein was expressed in E. coli and analyzed for its antigenic activity. Among those 7 clinically diagnosed patients' sera , there were 5 positive and 2 negative in reaction with His-fusion protein. All of the 20 healthy persons' sera and rabbit anti-OC43 and 229E were of negative reaction with His-fusion protein. The animals immunized with His-fusion protein have produced multi-clonal antibody. The His-fusion protein could specially react with clinically diagnosed SARS patients' sera and the animals immunized with His-fusion protein could produce specifically multi-clonal antibody, but it could not react with the sera of healthy persons and the rabbit anti-OC43 and 229E.展开更多
文摘To test the antigenic activity of M protein (Mc protein) in the inner membrane of SARS-CoV, SARS-CoV Mc protein's bases locating inside the membrane were cloned, the His-fusion protein was expressed in E. coli and analyzed for its antigenic activity. Among those 7 clinically diagnosed patients' sera , there were 5 positive and 2 negative in reaction with His-fusion protein. All of the 20 healthy persons' sera and rabbit anti-OC43 and 229E were of negative reaction with His-fusion protein. The animals immunized with His-fusion protein have produced multi-clonal antibody. The His-fusion protein could specially react with clinically diagnosed SARS patients' sera and the animals immunized with His-fusion protein could produce specifically multi-clonal antibody, but it could not react with the sera of healthy persons and the rabbit anti-OC43 and 229E.
