Objectives： To investigate the role of prolyl 4-hydroxylase beta polypeptide （P4HB） expressed in lung carcinoma and the intervention effect of Yiqi Chutan Formula （益气除痰方, YQCTF）. Methods： Lung carcinoma mod...Objectives： To investigate the role of prolyl 4-hydroxylase beta polypeptide （P4HB） expressed in lung carcinoma and the intervention effect of Yiqi Chutan Formula （益气除痰方, YQCTF）. Methods： Lung carcinoma model was established by subcutaneously inoculating LEWIS lung carcinoma cells in C57BL/6J mice. The differential expression of P4HB protein between the YQCTF （3.0 g/kg, gavage, once daily, 21 days） group and the control group was acquired by a 2 fluorescence difference gel electrophoresis （2D-DIGE）, verified by Westem blotting and identified by matrix-assisted laser desorption ionization time of flight mass spectrometry （MALDI-TOF/＇IOF-MS）. The expression of P4HB and P4HB mRNA in cultured A549 cells from cisplatin （DDP） 1.5μg/mL group and 15% serum combined with DDP 1.5 μg/mL group were detected by cellular immunohistochemistry and reverse chain reaction, respectively. Results： The proteomics research discovered that one-third of differential proteins including P4HB were decreased in the YQCTF group （P〈0.01）. Clinical pathology and tissue microarray studies showed that P4HB expression in lung cancer tissue was stronger than adjacent tissues and normal lung epithelial （P〈0.01）. In the YQCTF and DDP combined groups, the expression of P4HB and P4HB mRNA in A549 cell were decreased significantly （P〈0.01）. Conclusion： YQCTF could inhibit the LEWIS lung carcinoma＇s growth, decrease the expression of P4HB in LEWIS lung carcinoma and A549 cells. YQCTF might take effect through regulating P4HB in endoplasmic reticulum to inhibit the incidence and growth process of lung cercinoma.展开更多
基金Supported by the National Natural Science Fundation of China(No.30772862)Guangdong Natural Science Fund(No.2010-3)Project of Guangdong University Talent Fund(No.2010-79)
文摘Objectives： To investigate the role of prolyl 4-hydroxylase beta polypeptide （P4HB） expressed in lung carcinoma and the intervention effect of Yiqi Chutan Formula （益气除痰方, YQCTF）. Methods： Lung carcinoma model was established by subcutaneously inoculating LEWIS lung carcinoma cells in C57BL/6J mice. The differential expression of P4HB protein between the YQCTF （3.0 g/kg, gavage, once daily, 21 days） group and the control group was acquired by a 2 fluorescence difference gel electrophoresis （2D-DIGE）, verified by Westem blotting and identified by matrix-assisted laser desorption ionization time of flight mass spectrometry （MALDI-TOF/＇IOF-MS）. The expression of P4HB and P4HB mRNA in cultured A549 cells from cisplatin （DDP） 1.5μg/mL group and 15% serum combined with DDP 1.5 μg/mL group were detected by cellular immunohistochemistry and reverse chain reaction, respectively. Results： The proteomics research discovered that one-third of differential proteins including P4HB were decreased in the YQCTF group （P〈0.01）. Clinical pathology and tissue microarray studies showed that P4HB expression in lung cancer tissue was stronger than adjacent tissues and normal lung epithelial （P〈0.01）. In the YQCTF and DDP combined groups, the expression of P4HB and P4HB mRNA in A549 cell were decreased significantly （P〈0.01）. Conclusion： YQCTF could inhibit the LEWIS lung carcinoma＇s growth, decrease the expression of P4HB in LEWIS lung carcinoma and A549 cells. YQCTF might take effect through regulating P4HB in endoplasmic reticulum to inhibit the incidence and growth process of lung cercinoma.
