Relationship between Different Pronuclear Patterns and Potential of Embryo Development and Pregnancy

Objective To explore the relationship between the patterns of pronucleus and embryo development and pregnancy potential in the pronuclear stageMethods According to the number and distribution of nucleolar precursor bodies, the embryos at pronuclear stage were classified into 6 pronuclear patterns from 0 to 5, 16 - 18 h after in vitro fertilization (IV F) or intracytoplasmic sperm injection (ICSI). For each, pattern, the subsequent embryonic morphology and the pregnancy rate were analyzed.Results Embryos of Pattern 0 developed to significantly more embryos with good quality and higher pregnancy potential than the embryos developing from other patterns (83. 14% and 76. 11% respectively, P<0. 05). The pregnancy rate was decreased as less embryos of Pattern 0 were transferred . The pregnancy rate of the groups of only Pattern 0, with Pattern 0, and without Pattern 0 were 48. 08% , 32. 14% and 21. 28% respectively (P<0. 05).Conclusions The pronuclear patterns are of the predictive value of embryo development and pregnancy potential, which can be used as a new tool for the selection of embryos in IVF and ICSI.

原核期胚胎的原核模式与胚胎发育及妊娠关系的研究

目的:探讨原核期胚胎的原核模式与胚胎发育及妊娠能力的关系。方法:在IVF/ICSI后16～18 h,根据原核期胚胎的核仁前体数目和分布,将其分为模式0～5;并进行随后的胚胎形态学评估和计算妊娠率。结果:模式0的优良胚胎率(83.13％)高于其它各模式之和(76.11％,P<0.05);单纯移植模式0、含模式0和不含模式0组的妊娠率分别为48.08％、32.14％和21.28％,三组间差异有显著性(P<0.05)。结论:原核期胚胎的原核模式对预测胚胎质量和种植能力有一定参考价值。