期刊文献+
共找到10篇文章
< 1 >
每页显示 20 50 100
虾夷马粪海胆(Strongylocentrotus intermedius)体腔液的酚氧化酶活性分析 被引量:6
1
作者 王轶南 刘学伟 +1 位作者 刘艳萍 常亚青 《中国农业科技导报》 CAS CSCD 2011年第2期116-120,共5页
分别测定虾夷马粪海胆血浆和体腔细胞溶解上清液(CLS)中的酚氧化酶(PO)活性以及不同刺激物对其酚氧化酶原系统(proPO)的激活效果,并初步分析了患病虾夷马粪海胆体腔液中酚氧化酶活性的变化。结果显示:虾夷马粪海胆体腔液的PO主要分布于... 分别测定虾夷马粪海胆血浆和体腔细胞溶解上清液(CLS)中的酚氧化酶(PO)活性以及不同刺激物对其酚氧化酶原系统(proPO)的激活效果,并初步分析了患病虾夷马粪海胆体腔液中酚氧化酶活性的变化。结果显示:虾夷马粪海胆体腔液的PO主要分布于血浆中,且个体差异较大,血浆中PO活性为48.28±6.69U,CLS中PO活性为2.24±1.81 U;proPO存在于体腔细胞中,含量较少,其被1 mg/mL Trypsin及100 mmol/LMgCl2激活后,PO活性分别提高2.24 U和7.86 U,LPS、SDS、CaCl2和甲醇等对酚氧化酶原无明显激活效果;虾夷马粪海胆感染细菌后体腔液的酚氧化酶活性为12.90±2.03 U,相较于健康海胆(30.63±2.21 U)显著降低(P<0.05),而在100 mmol/L MgCl2作用下酚氧化酶活性提高4.19±1.96 U,相较于健康海胆(2.76±1.15U)明显升高(P<0.05)。研究结果为虾夷马粪海胆及其他棘皮动物的免疫防御机制的深入研究提供了基础。 展开更多
关键词 虾夷马粪海胆 体腔液 酚氧化酶(PO) 酚氧化酶原(proPO)
下载PDF
实时荧光定量PCR检测凡纳滨对虾proPO基因标准曲线构建 被引量:2
2
作者 孙双双 王亚军 +1 位作者 唐杰 刘必谦 《生物技术通报》 CAS CSCD 北大核心 2011年第2期131-135,共5页
酚氧化酶原(proPO)系统属于一种酶联级系统,在凡纳滨对虾抵抗病原菌侵袭过程中具有重要作用。Real-time PCR检测技术已经成为国际上检测基因表达通用的方法,能对低丰度mRNA水平进行定量,可在体外对细胞活性进行评估,选择该方法进行外界... 酚氧化酶原(proPO)系统属于一种酶联级系统,在凡纳滨对虾抵抗病原菌侵袭过程中具有重要作用。Real-time PCR检测技术已经成为国际上检测基因表达通用的方法,能对低丰度mRNA水平进行定量,可在体外对细胞活性进行评估,选择该方法进行外界环境因子胁迫下的凡纳滨对虾(Litopenaeus vannamei)组织proPO表达分析。在实时荧光定量PCR中,标准曲线法是最为简单、准确的定量方法。为了快速、准确的定量proPO基因在逆境下的表达水平,构建了正确、可靠的proPO基因表达标准品质粒和标准曲线。结果表明,该法所构建的标准曲线能够保证数据的精确性,满足实时荧光定量试验的要求,完全可以作为proPO基因荧光定量检测的参照标准。 展开更多
关键词 凡纳滨对虾 proPO基因 标准曲线 荧光定量PCR
下载PDF
利用免疫佳预防养殖甲壳动物的传染性疾病(下) 被引量:1
3
作者 陈昌福 张松柏 《渔业致富指南》 2004年第18期65-66,共2页
关键词 免疫预防 甲壳动物 传染性疾病 proPO活化系统
下载PDF
斑节对虾酚氧化酶原在大肠杆菌中的表达 被引量:1
4
作者 韩凤丽 贡成良 +1 位作者 曹广力 薛仁宇 《常熟理工学院学报》 2009年第4期61-64,81,共5页
将斑节对虾酚氧化酶原(prophenoloxidase,proPO)基因克隆进pET28a(+),在大肠杆菌BL21菌株中诱导表达,SDS-PAGE和Western-blotting分析均能检测到一条分子量为79.4kDa的特异性条带,与推导的融合蛋白理论分子量82.4kDa基本相符;包涵体变... 将斑节对虾酚氧化酶原(prophenoloxidase,proPO)基因克隆进pET28a(+),在大肠杆菌BL21菌株中诱导表达,SDS-PAGE和Western-blotting分析均能检测到一条分子量为79.4kDa的特异性条带,与推导的融合蛋白理论分子量82.4kDa基本相符;包涵体变性后对经Ni-NTA agarose纯化的变性液进行复性,表现出0.3851U/mg.min的PO活性;不同条件下的诱导表达结果显示,18℃时诱导培养能检测到目的蛋白的可溶性表达,经诱导9h后可溶性表达比例达到最高,约占菌体可溶性蛋白总量的5.56%;可溶性表达的目的蛋白纯化后经胰酶消化,可检测到分子量为60kDa、42.7kDa的特异性条带,并表现出0.4249U/mg.min的PO活性. 展开更多
关键词 蛋白纯化 proPO 原核表达 酶活性
下载PDF
Multivariate analysis by Cox Proportional Hazards Model on prognoses of patients with bile duct carcinoma after resection
5
作者 何平 石景森 +1 位作者 陈武科 王作仁 《Chinese Medical Journal》 SCIE CAS CSCD 2002年第10期98-101,153,共5页
To evaluate the influence of various clinicopathologic factors on the survival of patients with bile duct carcinoma after curative resection Methods A retrospective analysis was performed on 86 cases of bile duct ca... To evaluate the influence of various clinicopathologic factors on the survival of patients with bile duct carcinoma after curative resection Methods A retrospective analysis was performed on 86 cases of bile duct carcinoma treated from January 1981 to September 1995 Fifteen clinicopathologic factors that could possibly influence survival were selected A multivariate analysis of these individuals was performed using the Cox Proportional Hazards Model Results The overall cumulative survival rate was 73% for 1 year, 32% for 3 years and 19% for 5 years The results of univariate analysis showed that the major significant prognostic factors for influencing survival of these patients were type of histological lesion, lymph node metastasis, pancreatic invasion, duodenal invasion, perineural invasion, macroscopic vessel involvement, resected surgical margin and depth of cancer invasion ( P 【0 05) Pancreatic invasion, perineural invasion and lymph node metastases were the three most important prognostic factors determined by multivariate analysis using the Cox Proportional Hazards Model Conclusion Pancreatic invasion, perineural invasion and lymph node metastases are the most important prognostic factors for bile duct carcinoma after curative resection 展开更多
关键词 biliary tract neoplasms · Cox Propo rtional Hazards Model · prognosis
全文增补中
Modulation of immune responses of Rhynchophorus ferrugineus (Insecta: Coleoptera)induced by the entomopathogenic nematode Steinernema carpocapsae (Nematoda: Rhabditida) 被引量:1
6
作者 Maristella Mastore Vincenzo Arizza +1 位作者 Barbara Manachini Maurizio F. Brivio 《Insect Science》 SCIE CAS CSCD 2015年第6期748-760,共13页
Aim of this study was to investigate relationships between the red palm weevil (RPW) Rhynchophorusferrugineus (Olivier) and the entomopathogenic nematode Stein- ernema carpocapsae (EPN); particularly, the work w... Aim of this study was to investigate relationships between the red palm weevil (RPW) Rhynchophorusferrugineus (Olivier) and the entomopathogenic nematode Stein- ernema carpocapsae (EPN); particularly, the work was focused on the immune response of the insect host in naive larvae and after infection with the EPN. Two main immunological processes have been addressed: the activity and modulation of host prophenoloxidase- phenoloxidase (proPO) system, involved in melanization of not-self and hemocytes recog- nition processes responsible for not-self encapsulation. Moreover, immune depressive and immune evasive strategies of the parasite have been investigated. Our results suggest that RPW possess an efficient immune system, however in the early phase of infection, S. carpocapsae induces a strong inhibition of the host proPO system. In addition, host cell- mediated mechanisms of encapsulation, are completely avoided by the parasite, the elusive strategies orS. carpocapsae seem to be related to the structure of its body-surface, since induced alterations of the parasite cuticle resulted in the loss of its mimetic properties. S. carpocapsae before the release of its symbiotic bacteria, depress and elude RPW immune defenses, with the aim to arrange a favorable environment for its bacteria responsible of the septicemic death of the insect target. 展开更多
关键词 ENCAPSULATION immune depression immune evasion proPO system Rhyn-chophorus ferrugineus Steinernema carpocapsae
原文传递
漫谈法国新法语教材《A propos de…》
7
作者 馨林 骊岭 《外语教学理论与实践》 1992年第3期36-38,32,共4页
我们自1991年下半年来法国格勒诺布尔大学法语学习中心访学半年多来,接触了不少法语教材。这些教材各具特色,各有千秋,确实在一定程度上反映了法国在不同时期的作为外语的法语教学的历史发展情况。最近又有一本书名为《A propos de…》... 我们自1991年下半年来法国格勒诺布尔大学法语学习中心访学半年多来,接触了不少法语教材。这些教材各具特色,各有千秋,确实在一定程度上反映了法国在不同时期的作为外语的法语教学的历史发展情况。最近又有一本书名为《A propos de…》(《关于…》)的新教材问世。说也巧,该书的第一作者D.ABRY正好是我们的导师,她擅长于法语教学法理论的教学与研究,积累了丰富的经验。当我们从导师D.ABRY手中接过她赠送的书时。 展开更多
关键词 法语学习 法语教学 A propos DE 教学法理论 彩色封面 格勒诺布尔 教师用书 第一作者 语言实践 一本
原文传递
Le retour du monstre du lock Ness——A propos de La Possibillité d'une le,nouveau roman de Michel Houellebecq
8
作者 Shen Dali 《法语学习》 2005年第6期76-78,共3页
关键词 d’une le nouveau roman de Michel Houellebecq Le retour du monstre du lock Ness A propos de La Possibillit LA
原文传递
Potash Business Contributes Less to CITIC Guoan
9
《China Chemical Reporter》 2010年第5期31-31,共11页
CITIC Guoan Information Industry Co., Ltd.(SZ: 000839) posted sales revenue of RMB1.968 billion and net profit of RMB612 millionin 2009.Its unit, Guoan Qinghai, had salesrevenue of RMB687 million, up 33.66% froma year... CITIC Guoan Information Industry Co., Ltd.(SZ: 000839) posted sales revenue of RMB1.968 billion and net profit of RMB612 millionin 2009.Its unit, Guoan Qinghai, had salesrevenue of RMB687 million, up 33.66% froma year earlier, and earned RMB206 million,down 19%.Guoan Qinghai’s net income accountedfor about a third of CITIC Guoan’stotal.In 2008, the proportion was more 展开更多
关键词 earned EARLIER REVENUE PROFIT INCOME QINGHAI propo
全文增补中
China Sets Target for Strategic Emerging Industries
10
《China Chemical Reporter》 2010年第21期5-5,共11页
China’s State Council on October 10th announceda plan to accelerate the developmentof strategic emerging industries. Theaim is that the industrial added value ofthese emerging industries will account forabout 8% of C... China’s State Council on October 10th announceda plan to accelerate the developmentof strategic emerging industries. Theaim is that the industrial added value ofthese emerging industries will account forabout 8% of China’s GDP by 2015 and15% by 2020.The proportion is currently around 3%.The target shows the ambitions of the Chinesegovernment to alter economic 展开更多
关键词 STRATEGIC EMERGING currently STRATEGIC ALTER propo
全文增补中
上一页 1 下一页 到第
使用帮助 返回顶部