In silico prospective analysis of the medicinal plants activity on the CagA oncoprotein from Helicobacter pylori

BACKGROUND Colonization with Helicobacter pylori(H.pylori)has a strong correlation with gastric cancer,and the virulence factor CagA is implicated in carcinogenesis.Studies have been conducted using medicinal plants with the aim of eliminating the pathogen;however,the possibility of blocking H.pylori-induced cell differentiation to prevent the onset and/or progression of tumors has not been addressed.This type of study is expensive and time-consuming,requiring in vitro and/or in vivo tests,which can be solved using bioinformatics.Therefore,prospective computational analyses were conducted to assess the feasibility of interaction between phenolic compounds from medicinal plants and the CagA oncoprotein.AIM To perform a computational prospecting of the interactions between phenolic compounds from medicinal plants and the CagA oncoprotein of H.pylori.METHODS In this in silico study,the structures of the phenolic compounds(ligands)kaempferol,myricetin,quercetin,ponciretin(flavonoids),and chlorogenic acid(phenolic acid)were selected from the PubChem database.These phenolic compounds were chosen based on previous studies that suggested medicinal plants as non-drug treatments to eliminate H.pylori infection.The three-dimensional structure model of the CagA oncoprotein of H.pylori(receptor)was obtained through molecular modeling using computational tools from the I-Tasser platform,employing the threading methodology.The primary sequence of CagA was sourced from GenBank(BAK52797.1).A screening was conducted to identify binding sites in the structure of the CagA oncoprotein that could potentially interact with the ligands,utilizing the GRaSP online platform.Both the ligands and receptor were prepared for molecular docking using AutoDock Tools 4(ADT)software,and the simulations were carried out using a combination of ADT and AutoDock Vina v.1.2.0 software.Two sets of simulations were performed:One involving the central region of CagA with phenolic compounds,and another involving the carboxy-terminus region of CagA with phenolic compounds.The receptor-ligand complexes were then analyzed using PyMol and BIOVIA Discovery Studio software.RESULTS The structure model obtained for the CagA oncoprotein exhibited high quality(C-score=0.09)and was validated using parameters from the MolProbity platform.The GRaSP online platform identified 24 residues(phenylalanine and leucine)as potential binding sites on the CagA oncoprotein.Molecular docking simulations were conducted with the three-dimensional model of the CagA oncoprotein.No complexes were observed in the simulations between the carboxy-terminus region of CagA and the phenolic compounds;however,all phenolic compounds interacted with the central region of the oncoprotein.Phenolic compounds and CagA exhibited significant affinity energy(-7.9 to-9.1 kcal/mol):CagA/kaempferol formed 28 chemical bonds,CagA/myricetin formed 18 chemical bonds,CagA/quercetin formed 16 chemical bonds,CagA/ponciretin formed 13 chemical bonds,and CagA/chlorogenic acid formed 17 chemical bonds.Although none of the phenolic compounds directly bound to the amino acid residues of the K-Xn-R-X-R membrane binding motif,all of them bound to residues,mostly positively or negatively charged,located near this region.CONCLUSION In silico,the tested phenolic compounds formed stable complexes with CagA.Therefore,they could be tested in vitro and/or in vivo to validate the findings,and to assess interference in CagA/cellular target interactions and in the oncogenic differentiation of gastric cells.

Analysis on Prospecting Potential of Tungsten Polymetallic Ore in Haergeng of Gonghe County, Qinghai Province

The discovery of Gonghe County Haergeng tungsten polymetallic deposit is one of the most important tungsten deposits discovered in Qinghai Province in recent years.It has important theoretical and practicalsignificance.1 Regional geological background The research area lies in the northwest edge of

The Research Progress and Prospect Analysis of Germ-Remaining Rice Whitener

In this paper, the backdrop and significance of the research of germ-remaining rice whitener firstly were expatiated. Then, the study progress in China and overseas was summarized. Lastly, the research prospect of this kind of whitener in China was analyzed. It is hoped that it can coach the design of germ-remaining rice whitener.

Development Situation and Prospect Analysis of Biodegradable Film Industry

Application and promotion of biodegradable plastic film in modern agricultural production construction have become trend and direction of agricultural development.As important production material,biodegradable plastic film not only effectively changes agricultural production mode but also effectively decreases environmental pollution.Accompanied by degradation of new material,upgrading of optimizing process,and infiltration and blending of science and technology in new plastics industry,new biodegradable plastic films have been developed and popularized effectively and rapidly.In this paper,starting from development situation and prospect of degradable plastic film in China,natural-based biodegradable plastics,petroleum-based biodegradable plastics and biological-based biodegradable plastics are introduced in detail from basic material of plastics.The advantages and disadvantages of additive biodegradable film and fully biodegradable film are scientifically analyzed,and seasonal and periodic characteristics of biodegradable plastic film industry are elaborated.Moreover,disadvantages of biodegradable plastic film industry development are explored from technical barrier,brand and channel barriers,and talent barrier.Finally,development trend of biodegradable plastic film industry in China is predicted and analyzed scientifically.The research could provide guidance for the development of biodegradable plastic film industry in China ,research of biodegradable plastic film technology,and demonstration and extension of biodegradable plastic film application.

Chemical Analysis of Water and the Resource of Br,K Prospects of Oilfield Brines from Ordovician and Carboniferous in Tarim,China

China is lack of bromine and potassium seriously.Oilfield brines is the headline goal of bromine and potassium resources exploration.Applicants grab 24oilfield brines samples from various wells of Ordovician

Quantitative evaluation of hepatitis B virus mutations and hepatocellular carcinoma risk:a meta-analysis of prospective studies

Background： The temporal relationship between hepatitis B virus （HBV） mutations and hepatocellular carcinoma （HCC） remains unclear. Methods： We conducted a meta-analysis including cohort and nested case-control studies to prospectively examine the HCC risk associated with common variants of HBV in the PreS, Enhancer Ⅱ, basal core promoter （BCP） and precore regions. Pertinent studies were identified by searching PubMed, Web of Science and the Chinese Biological Medicine databases through to November 2014. Study-specific risk estimates were combined using fixed or random effects models depending on whether significant heterogeneity was detected. Results： Twenty prospective studies were identified, which included 8 cohort and 12 nested case-control studies. There was an increased risk of HCC associated with any PreS mutations with a pooled relative risk （RR） of 3.82 [95% confidence interval （CI）： 2.59-5.61]. The pooled-RR for PreS deletion was 3.98 （95% CI： 2.28-6.95）, which was higher than that of PreS2 start codon mutation （pooled-RR=2.63, 95% CI： 1.30-5.34）. C1653T in Enhancer Ⅱ was significantly associated with HCC risk （pooled-RR=l.83; 95% CI： 1.21-2.76）. For mutations in BCP, statistically significant pooled-RRs of HCC were obtained for T1753V （pooled- RR=2.09; 95% CI： 1.49-2.94） and AI762T/G1764A double mutations （pooled-RR=3.11; 95% CI： 2.08- 4.64）. No statistically significant association with HCC risk was observed for G1896A in the precore region （pooled-RR=0.77; 95% CI： 0.47-1.26）. Conclusions： This study demonstrated that PreS mutations, C1653T, T1753V, and A1762T/G1764A, were associated with an increased risk of HCC. Clinical practices concerning the HCC risk prediction and diagnosis may wish to focus on patients with these mutations.

Evolution and prospect of China's rural development policy: A policy text analysis of the No.1 Central Documents

By combing 20 documents of the Central Committee on the historical evolution of rural development policies since 1982, we hold that historical evolution has undergone reforms, adjustments, modernization developments and new ideas, and the path of reform experienced economic recovery, industrial nurturing agriculture, agriculture modernization and rural revitalization. The study found that: farmers' income has always been the focus of attention; agricultural production has shifted from total demand to green ecology; urban and rural resource elements are not well-organized, resulting in internal contradictions. The implementation of the rural revitalization strategy is an important measure to fundamentally solve the rural development problems in the new era.

Geological mapping and mining prospecting in the Aouli inlier(Eastern Meseta,Morocco)based on remote sensing and geographic information systems(GIS)

Landsat 8 Oli,ASTER,and Sentinel 2A satellite images processing was used to map geological formations,lineaments and hydrothermal alteration minerals in the Aouli inlier,as a case study to illustrate the application of digital images processing and Geographic Information System(GIS)in geological mapping and mining prospecting.Principal Component Analysis(PCA)applied to the Landsat images allowed good lithological discrimination and contributed to the updating of available geological maps.The Automatic lineament extraction from Sentinel images revealed the main tectonic structures affecting Aouli inlier.The ratio bands(b5+b7)/b6 and the false color composite(b4/b6,b2/b1,b3/b2)allowed the hydrothermal alteration minerals mapping from Aster images.Combined with available geological data and field observations,the satellite derived data were integrated and analyzed in a GIS software to establish mining prospecting guides.The results showed that the anomaly zones are intimately linked to NNE-SSW and NW-SE oriented faults and to highly fractured areas developing argillic and Fe rich alterations.Verified via field survey,this approach was successfully applied to the Aouli inlier to rapidly target potential areas to be explored in the tactical phase.This provides a model for future prospecting efforts for similar mineral deposits in other areas.

Hydrocarbon Prospectivity and Risk Assessment of “Bob” Field Central Swamp Depobelt, Onshore Niger Delta Basin, Nigeria

This paper evaluates the hydrocarbon prospectivity and play risks of “Bob” field in Niger Delta Basin, Nigeria. The aim is to enhance exploration success through improved approach/technique by incorporating risk analysis that previous studies have not fully considered. This approach combines a set of analyses including stratigraphic/structural, amplitude, petrophysical parameter, volumetric and play risk using a suite of well logs and 3D seismic data. Maximum amplitude anomaly map extracted on the surfaces of delineated 3 reservoirs revealed 6 prospects, namely: Dippers, Cranes, Turacos, Nicators, Jacanas and Pelicans with hydrocarbon accumulation. Petrophysical analysis showed ranges of values for porosity, permeability and water saturation of 0.21 to 0.23, 158.96 to 882.39 mD, and 0.07 to 0.11, respectively. The various prospects yielded the following stock tank volumes 12.73, 6.84, 3.84, 11.32, 7.42 and 4.76 Million barrels (Mbls) each respectively in a column of 66 ft reservoir sand in the study area. Play risk analysis results gave: Pelicans and Nicators (low), Turacos and Dippers (moderate), while Jacanas and Cranes show high risk with minimal promise for good oil accumulation. The prospects possess good reservoir petrophysical properties with low to moderate risk, thus, viable for commercial hydrocarbon production, which increases confidence in management decisions for production.

基于靴襻渗流模型的国际信息通信产业贸易前景分析

信息通信技术(ICT)产业是国际数字经济发展的核心产业,交互贯通的国际ICT产业贸易网络能够客观反映出ICT产业贸易的时空分异格局。本文运用复杂网络分析方法探测国际ICT产业贸易网络的基础拓扑特征及中心性特征,同时运用靴襻渗流模型讨论ICT产业贸易的危机传播过程。研究发现,国际ICT产业整体贸易强度有所扩大,呈现出明显的区域集中化趋势,但贸易联系的整体活跃度、贸易范围和网络传输效率都明显降低;主要ICT产业贸易国(地区)整体的危机传染力高于以往,防控核心贸易国(地区)的点危机与预防核心板块的区域性联动危机相结合将是阻止危机全球蔓延的有效举措。

我国历史文化村落保护研究的内容综述与工作前瞻

历史文化村落既是历史文化遗产保护的核心要素,又是我国乡村振兴战略推进的工作对象。文章借助CiteSpace分析工具,对相关文献进行关键信息的检索、提取、分析和研判。分析结果表明,我国历史文化村落研究大体可划分为依附研究、专属研究和拓展研究3个阶段,主要围绕“本体机理的认知解读”“文化遗产的保护转型”“社会体系的组织治理”和“产业发展的创新规划”4大主题展开讨论,体现从“实体历史空间”到“复合多元要素”的对象演变、从“孤立对象保护”到“区域统筹发展”的目标拓展、从“单科定性”到“交叉定量”的方法进化等研究特征。最后,面对乡村振兴的机遇与乡村收缩的挑战,从研究视角、保护理论、实践方法等方面提出工作展望,以期为新时代我国历史文化村落保护实践提供科学的理论支持。

“双创”培养模式驱动下食品质量与安全专业人才培养需求分析

在健康中国的战略背景下,食品质量与安全行业的就业环境发生了巨大变化,对创新创业型高素质人才的需求不断增加。该文通过分析双创教育在高等教育和经济社会发展中的重要作用,特别是双创教育对食品质量与安全专业的重要性及不足分析,进一步探讨双创教育对食品质量与安全专业人才培养方向和发展前景。

基于知识图谱的我国农业数字化研究现状与未来展望

以CNKI数据库中主题为“农业数字化”的相关文献作为数据来源,运用CiteSpace软件对农业数字化领域研究现状进行可视化分析。结果表明,农业数字化领域研究热度日趋提升,文献数量在2020年呈现井喷式增长,并逐渐建立起较有凝聚力的科研群体;该领域研究热点不断拓展,从农业机械、农业信息化等延申至数字农业、数字技术、智慧农业等方面;数字技术成为该领域今后研究的重点方向。最后,未来农业数字化发展仍面临着整体水平不高、区域发展不平衡、基础设施薄弱等问题需进一步探索与完善。

文化翻译视角下壮族体育文化外宣翻译文献分析与研究展望

随着我国综合实力和国际地位不断攀升,民族自信和文化自信日益受到尊崇。梳理近20年文献综述发现,现阶段壮族体育文化的研究成果虽初具规模,但大多集中在溯源、挖掘、整理、传承发展等方面,在外宣方面的研究较少,且不成体系,文化表达、翻译策略等方面存在短板。该文在文化翻译理论指导下,一方面,通过对相关文献的梳理,发现研究短板,提出研究建议与展望。另一方面,为探讨如何选取适宜的外宣翻译策略,即如何保持壮族特色又能提高文化传播适应性的研究而提供研究依据,从而加快广西民族特色文化的传播,使得壮族体育文化能更好地造福人类健康。

低碳氨燃料生命周期碳排计算和应用前景探究

文章梳理了低碳氨的燃料属性和特点,测算了目前技术水平下低碳氨生命周期碳排因子,对低碳氨与煤制氨的成本进行了分析,指出随着技术进步成本进一步下降,风光发电制低碳氨燃料经济性将逐步体现,未来具有发展空间。低碳氨生命周期碳排因子生产环节排放占90%以上,生产环节中碳排基本上是制氢和制氮用电导致,因此降低碳排主要方向是减少生产环节用电量和尽可能增加绿电使用量。

酒精摄入与脑卒中发病风险的剂量反应Meta分析

背景脑卒中作为一种重大慢性非传染性疾病严重影响国民健康,给患者、家庭和社会造成沉重负担。饮酒现象在我国普遍存在,酒精摄入与脑卒中发病之间存在密切关系,但酒精摄入剂量和脑卒中发病关系仍存有争议。目的探讨酒精摄入量与脑卒中发病风险的关系。方法计算机检索PubMed、Web of Science、Cochrane Library、Embase、中国知网、维普网、万方数据知识服务平台和中国生物医学文献数据库,收集关于酒精与脑卒中发病风险的前瞻性队列研究,检索时限均为建库至2023年12月。文献筛选、数据提取、文献质量评价由2位研究者独立进行。应用Stata/MP 17.0软件进行剂量反应Meta分析。结果共纳入16篇文献,共548595例研究对象。Meta分析结果显示,酒精摄入与脑卒中发病风险相关(RR=1.17,95%CI=1.10~1.26,P<0.05)。亚组分析结果,每天酒精摄入<20 g脑卒中发病风险降低10%(RR=0.90,95%CI=0.85~0.95,P<0.05);酒精摄入>40 g脑卒中发病风险增加35%(RR=1.35,95%CI=1.23~1.49,P<0.05);酒精摄入出血性脑卒中风险增加49%(RR=1.49,95%CI=1.14~1.95,P<0.05),酒精摄入缺血性脑卒中风险增加20%(RR=1.20,95%CI=1.00~1.43,P<0.05);亚洲人群酒精摄入脑卒中发病风险增加27%(RR=1.27,95%CI=1.14~1.40,P<0.05);男性酒精摄入脑卒中风险增加19%(RR=1.19,95%CI=1.09~1.29,P<0.05)。剂量反应Meta分析结果,酒精摄入与脑卒中发病风险之间呈J型非线性关系(P=0.018),与从不饮酒者相比,饮酒者摄入酒精剂量的脑卒中相对风险比分别为1 g/d:RR=0.97,95%CI=0.96~0.98;2 g/d:RR=0.96,95%CI=0.94~0.97;3 g/d:RR=0.95,95%CI=0.93~0.97;4 g/d:RR=0.94,95%CI=0.91~0.96;5 g/d:RR=0.91,95%CI=0.88~0.94;6 g/d:RR=0.90,95%CI=0.86~0.93;7 g/d:RR=0.88,95%CI=0.84~0.92;8 g/d:RR=0.88,95%CI=0.83~0.92;9 g/d:RR=0.88,95%CI=0.83~0.92;10 g/d:RR=0.88,95%CI=0.83~0.93;11 g/d:RR=0.88,95%CI=0.83~0.93;12 g/d:RR=0.90,95%CI=0.85~0.95;13 g/d:RR=0.91,95%CI=0.85~0.95;14 g/d:RR=0.92,95%CI=0.86~0.95;15 g/d:RR=0.93,95%CI=0.86~0.96;16 g/d:RR=0.95,95%CI=0.88~0.96;17 g/d:RR=0.96,95%CI=0.88~0.97;18 g/d:RR=0.98,95%CI=0.89~0.97;19 g/d:RR=0.98,95%CI=0.89~0.98;20 g/d:RR=0.99,95%CI=0.90~0.99。饮酒者平均每天酒精摄入量<20 g表现为脑卒中发病风险降低(P<0.001)。结论酒精摄入量与脑卒中风险间具有J型非线性的剂量反应关系,适量酒精摄入与脑卒中风险呈负相关,平均每天酒精摄入7~11 g时脑卒中风险最低。

基于PROSPECT模型的植物叶片干物质估测建模研究

为了快速、准确估测植物叶片干物质含量,为作物长势及健康状况监控提供数据支撑,利用光谱分析技术探讨了干物质含量敏感光谱波段提取方法及其估测建模方法。试验数据由叶片辐射传输模型PROSPECT在干物质含量(0.001~0.02)g·cm^(-2)范围内进行模拟,随机产生1000条400~2500nm的光谱曲线,其中600条光谱曲线用于建模研究、400条光谱曲线作为模型验证数据,同时应用叶片光学特性数据库LOPEX93中325条叶片光谱-干物质含量数据进行进一步验证。首先应用试验数据进行局部敏感性分析,初步得到叶片干物质敏感波段范围,再运用改进Sobol算法进行全局敏感性分析,提取了干物质含量敏感的光谱波段范围,在此敏感波段范围运用波段组合算法计算归一化植被指数NDVI与叶片干物质含量相关系数,优选了4组相关性大的波段组合建立归一化干物质指数NDMI_((1644,1719))、NDMI_((1871,2294))、NDMI_((2150,2271))、NDMI_((1496,2282))用于干物质含量估测建模。结果表明:NDMI_((1644,1719))和NDMI_((1871,2294))模型中三次多项式形式(cubic)效果最佳、NDMI_((1496,2282))模型中幂指数形式(power)效果最佳,三者中NDMI_((1871,2294))的三次多项式模型最优,决定系数R^2为0.837,对叶片干物质含量具有较好的估测能力。