Detection of Phaeocystis globosa using sandwich hybridization integrated with nuclease protection assay(NPA-SH)

Phaeocystis globosa Scherffel is one of the common harmful algae species in coastal waters of the southeastern China.In this study,sandwich hybridization integrated with nuclease protection assay(NPA-SH)was used to qualitatively and quantitatively detect P. globosa.Results showed that this method had good applicability and validity in analyzing the samples from laboratory cultures and from fields.The linear regression equation for P.globosa was obtained,and the lowest detection number of cells was 1.8×104 c...

Detection of Prorocentrum donghaiense using sandwich hybridization integrated with nuclease protection assay

Prorocentrum donghaiense is an important harmful algae bloom （HAB） causing creature in China＇s seas, and the conventional visual detection can not cope with long-term monitoring and highthroughput sampling projects. An assay for P. donghaiense with sandwich hybridization integrated with nuclease protection assay （NPA-SH） was established. Tests with mixed samples and spiked field ones confirmed its good specificity and sensitivity. The cell number of P. donghaiense correlated well with the optical density, and the regression equation is y=4× 10^- 6x＋ 0.694 9, in which x is the cell number, and y is the optical density, with r2=0.953 5. These results show that the NPA-SH method has good feasibility in the detection of P. donghaiense. Results of NPA-SH and microscopy are excellent for each sample. The NPA-SH method was a simple way in quantitative detection of P. donghaiense, and the whole process could be finished in about six hours, which provided a new approach in high-throughput sampling and long-term monitoring of P. donghaiense.

Architecture Design for Integrated Wide Area Protection and Control Systems

This paper firstly reviews the recent development in power system protection and control, with special attention paid to the wide-area and integrated protection, in order to look into future development of integration of protection and control for smart grids. This paper mainly reports on the development of integrated wide area protection and control for power systems. The concept of integrated wide area protection and control is introduced, in which a hierarchical protection and control system provides the protection and control of wide area or regional power substations/ plants and their associated power network. The system is mainly divided into three levels, the local, the substation/plant and the wide area/regional protection and control. The integrated functions at each level are described in details with an aim to develop an optimal coordination mechanism between the levels. One of the core elements in the system is the synchronised wide area communication network between the substations and the protection and control system, in which latest communication technology is employed. Another important player in the system is the wide area synchronized protection and control information platform, which not only enables the fusion three line of defence for power system protection and control, but also provides a perfect tool for the application of cloud computing to substations and power networks.

Study on Lightning Protection Integrated Service Management Platform in Yantai City

Via database technology, a perfect lightning protection integrated service work platform is established, thereby realizing paperless data management of routine business.

Simulation Studies of an Integrated Overcurrent Relay Protection Scheme for Distribution Systems

This paper presents an integrated protection technique for power distribution systems. A novel integrated protection scheme based on overcurrent protection technique for distribution system is described, in which a specially designed protection relay is installed at each substation of a network and responsible for the protection of every line sections connected to the substation busbar The conventional directional overcurrent and the new adaptive accelerated protection algorithms with multiple settings are implemented into the relay to cover all the protected line sections. The paper includes studies of a typical multi section distribution network to demonstrate the principle of the scheme. Studies show that the new scheme not only offer the new protection features for individual power line section, but also provide the characteristics of integrated protection.

Integrated Protection Scheme for Power Line

This paper presents an integrated relay and its associated protection scheme for power transmission lines.The concept of integrated protection is firstly introduced,in which a centralized protection system or relay provides the protection for multiple power plants or a substation.A novel integrated protection relay based on the combination of a number of protection principles is described.In the proposed scheme,the specially designed protection relays are installed at each substation of a network and are responsible for the protection of every line section connected to the substation busbar.The combined algorithms with multiple settings are implemented into the relay to cover all the protected line sections.Simulation studies show that the proposed protection scheme is able to produce desirable performance and may offer many advantages over the existing one and great potential for future applications.

Advanced Communication System in Substation for Integrated Protection

This paper proposes an advanced substation integrated protection communication system based on the latest development in power system.The integrated protection communication system within a substation comprises a number of major components such as the merging unit, communication network and integrated protection unit.The design and capability evaluation of the communication network based on IEC61850 are focused on.Results show that huge information from 4 merging units can be transmitted real-time via adopting 100 Mbit/s optical fiber in a substation,and the proposed communication network is able to achieve required performance.

Integrated Development of the Tourism and Protection of Ancient Towns

Local color is not only the cultural counter in the competition between cities,but the main cultural element that attracts foreign tourists.In recent years,many cities have regarded tourism development as a major mean of reviving ancient towns.The excavation of local culture,material reconstruction of local characteristics,and the resurgence and"reproduction"of local industries have become an important work in the tourism development of ancient towns.Apart from the discussion of the protection,inheritance,and touristification of tangible cultural heritage and intangible cultural heritage,this paper demonstrated the possibility of the integration and production capacity of local characteristic industry resources and analyzed the impact of the integrated development on the lifestyle and living atmosphere of residents in the ancient towns.In this paper,ways to realize the integrated development of the tourism and protection of ancient towns were included as follows:(1)to ensure the principal status of local residents and give full play to their initiatives;(2)to protect the local traditional way of life and improve the public service system;(3)to choose the appropriate development pattern and achieve the diversified development;(4)to improve service facilities in tourism and adjust the structure of tourism products.

Comparison of Thermo-Structural Responses for Integrated Thermal Protection Panels with Different Corrugated Core Configurations

To explore new light-weight integrated thermal protection system panel configuration and gain good insight into the responses mechanism,heat transfer and structural field analysis for one single-layer and four double-layer corrugated core panels were performed. The obtained the temperature,buckling,stress and deflection responses were compared,and the deflection and stress distributions as well as thermal buckling mode at the time were discussed for the considered configurations when the temperature difference between the top and bottom face sheet was maximum. The results demonstrated that the non-orthogonal and hat-stiffened double-layer structures provide superior performance to resist thermal buckling deformation in comparison with other configurations. The useful information is provided for the forthcoming optimization in which thermal buckling is considered as critical design driver.

ESD Protection Design and Characteristic Analysis of Advanced Process Integrated Circuit

The electrostatic discharge(ESD)phenomenon is very common,in daily life,many places will appear ESD phenomenon.However,ESD is a potential hazard for integrated circuits.This paper analyzes the ESD protection design and characteristics of advanced process integrated circuits,and puts forward personal views combined with experience,hoping to bring help to the people who pay attention to the ESD protection of integrated circuits.

Solutions to Right Conflicts and Integrated Protection of Diverse Interests in Real EstateDevelopment and Operation

In the development and operation of real estate, real estate development enterprises as the principal subject would often establish contractual relationships with subjects of diverse interests including removed households, banks, construction project contractors, and buyers. At times, conflicts of interests might occur among these contractual subjects requesting civil and commercial claims of the same subject matter based on agreements or legal rights. As for the causes of these conflicts and their solutions, a majority of existing researches still stay at the superficial causes from the perspective of civil and commercial law, and the solution of determining the order of priority of compensation of related rights. Due to the lack of research into deep-seated reasons and solutions from the perspective of economic law, the current legislative and judicial approaches to this kind of problems tend to give more protection to the rights of one subject while lack sufficient attention to other subjects. This paper attempts to carry out analyses and research on the causes and solutions to this kind of conflicts from the perspective of economic law, to achieve integrated protection of diverse interests in real estate development and operation so as to better safeguard the order in real estate development and operation and facilitate the healthy and ordered development of the real estate industry.

Integrated approach to colorectal anastomotic leakage: Communication, infection and healing disturbances

Colorectal anastomotic leakage(CAL) remains a major complication after colorectal surgery. Despite all efforts during the last decades, the incidence of CAL has not decreased. In this review, we summarize the available strategies regarding prevention, prediction and intervention of CAL and categorize them into three categories: communication, infection and healing disturbances. These three major factors actively interact during the onset of CAL. We aim to provide an integrated approach to CAL based on its etiology. The intraoperative air leak test, intraoperative endoscopy, radiological examinations and stoma construction mainly aim to detect and to prevent communication between the intra- and extra-luminal content. Other strategies including postoperative drainage, antibiotics, and infectious-parameter evaluation are intended to detect and prevent anastomotic or peritoneal infection. Most currently available interventions for CAL focus on the control of communication and infection, while strategies targeting the healing disturbances such as lifestyle changes, oxygen therapy and evaluation of metabolic biomarkers still lack wide clinical application. This simplified categorization may contribute to an integrated understanding of CAL. We strongly believe that this integrated approach should be taken into consideration during clinical practice. An integrated approach to CAL could contribute to a better understanding of the etiology of CAL and eventually better patient outcome.

INTEGRATED ANALYSIS APPROACHES TO ROCK MECHANICS PROBLEMS

In order to effectively cope with exponent increase of the complexity faced to the rock mechanics analysis problems and the large incompatibility existing between the information level required to model the rock mass and engineering and our obtainable information level at hand,the integrated approaches with intelligent characters are proposed. Many previous standard methods,such as precedent type analysis,rock classification,analytic method stress-based,basic numerical methods (BEM,FEM,DEM,hybrid),and their extended numerical methods (fully coupled) to be developed,can be selected respectively or integrated accordingly. It is alternative to develop basic/fully integrated system,and internet-based approaches. These novel methods can also be selected or integrated each other or with the standard methods to perform rock mechanics analysis. Some key techniques to develop these alternative methods are discussed. It may focus in future on developing fully integrated systems and internet-based approaches. Developing an environmental,virtual facility/space shall be firstly done for this collaborative research on internet.

Characteristics and Protection Approaches of Human Settlement in Ancient Villages of Jiangxi: A Case Study of Ancient Dongyuan Zengs' Village

Ancient village as a precious historical and cultural heritage of China is hailed as the "eco-museum" of classical folk cultures and "living fossil" of rural history culture. This paper elaborated human settlement characteristics of ancient Dongyuan Zengs' Village, Jinxi County from the perspectives of village environment, history evolution, village layout and village building, and explored the substantial content of village human settlement protection, and proposed specifi c approaches of protecting the human settlement, such as ancient village layout, history and culture, and provided references for protecting human settlement of the ancient villages.

ISSUES THAT ARE OF IMPORTANCE FOR THE INTERNATIONAL COMMISSION ON RADIOLOGICAL PROTECTION AS THE MILLENNIUM APPROACHES

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Redundant Data Detection and Deletion to Meet Privacy Protection Requirements in Blockchain-Based Edge Computing Environment

With the rapid development of information technology,IoT devices play a huge role in physiological health data detection.The exponential growth of medical data requires us to reasonably allocate storage space for cloud servers and edge nodes.The storage capacity of edge nodes close to users is limited.We should store hotspot data in edge nodes as much as possible,so as to ensure response timeliness and access hit rate;However,the current scheme cannot guarantee that every sub-message in a complete data stored by the edge node meets the requirements of hot data;How to complete the detection and deletion of redundant data in edge nodes under the premise of protecting user privacy and data dynamic integrity has become a challenging problem.Our paper proposes a redundant data detection method that meets the privacy protection requirements.By scanning the cipher text,it is determined whether each sub-message of the data in the edge node meets the requirements of the hot data.It has the same effect as zero-knowledge proof,and it will not reveal the privacy of users.In addition,for redundant sub-data that does not meet the requirements of hot data,our paper proposes a redundant data deletion scheme that meets the dynamic integrity of the data.We use Content Extraction Signature(CES)to generate the remaining hot data signature after the redundant data is deleted.The feasibility of the scheme is proved through safety analysis and efficiency analysis.

Participatory and Integrated Research in Mountainous Regions of Thailand and Vietnam:Approaches and Lessons Learned

Participatory and integrated research approaches employed by a long-term Thai- Vietnamese-German collaborative research program, ‘The Uplands Program’, that address the vicious circles of resource scarcity, environmental degrada- tion and rural poverty in mountainous regions of northern Thailand and northern Vietnam are discussed in this paper. We present two examples from the Thai component of the research program to show how different disciplines and stakeholders need to cooperate at different scales to make meaningful scientific contributions towards sustainable land use and rural development in mountainous regions. The case of resource conservation in the Thai highlands shows that local and scientific knowledge, conven- tional surveys and participatory modeling can be creatively combined. Integrated research on the potential of integrating fruit trees and associated technologies into mountain farming systems suggests that natural scientists have to work alongsideeconomists and social scientists to avoid harmful effects of purely technology-driven and productivity- enhancing approaches. The success of new technologies cannot be measured solely by adoption rates and yield increases, but also needs to take into account their long-term impact on various groups of farmers and the ecological, economic and social trade-offs that they entail. Technical and institutional innovations need to go hand in hand to provide viable livelihood opportunities for smallholder farmers in mountain watersheds. The major lesson learned from the first six years of our research in the mountains of Thailand and Vietnam is that conventional and participatory approaches are not antagonistic; if scientists from various disciplines and research paradigms are open-minded, the combination of both approaches can produce meaningful results that cater for the needs of both the academic community and local stakeholders in mountain environments.

Application of vertically integrated sampling approach to study of new production via ^(234)Th -^(238)U disequilibria

The spatial and temporal distributions of new production vary largely in different sea areas. To understand the level of new production in the sea area studied better, an estimate of new production must be obtained in large spatial and temporal scales. The ~234Th/ ~238U disequilibrium is an effective method for the study of new production. Two sampling strategies, vertically integrated sampling ap proach based on trapezoidal integration principle and discrete layer sampling approach, were compared in the studies of the xiamen Bay and the northern South China Sea. The scavenging fluxes and removal fluxes of ~234Th and the residence times for dissolved and particulate ~234Th were calculated. The coinci dent results from two Sampling approach suggest that vertically integrated sampling approach is not only effective and reliable, but also significantly reduces the number and volume of samples. It allows us to study new production by ba ^(234)Th - ^(238)U disequilibria in large spatial scale.

Reliability analysis for vertical integration of protection,measurement,merge unit,and intelligent terminal device

The reliability analysis of vertically integrated protection devices is crucial for designing International Electrotechnical Commission(IEC)61850-based substations.This paper presents the hardware architecture of a four-inone vertically integrated device and the information transmission path of each function based on the functional information transmission chain of protection devices,measurement and control devices,merging units,and intelligent terminals.Additionally,a reliability analysis model of the protection device and its protection system is constructed using the fault tree analysis method while considering the characteristics of each module of the vertically integrated device.The stability probability of the protection system in each state is analyzed by combining the state-transfer equations of line and busbar protection with a Markov chain.Finally,the failure rate and availability of the protection device and its protection system are calculated under different ambient temperatures using a 110 kV intelligent substation as an example.The sensitivity of each device module is analyzed.

DNA Barcoding of Insects and Its Direct Application for Plant Protection

The introduction of invasive insect pests across national borders has become a major concern in crop production. Accordingly, national plant protection organizations are challenge to reinforce their monitoring strategies, which are hampered by the weight and size of inspection equipment, as well as the taxonomic extensiveness of interrupted species. Moreover, some insect pests that impede farmer productivity and profitability are difficult for researchers to address on time due to a lack of appropriate plant protection measures. Farmers’ reliance on synthetic pesticides and biocontrol agents has resulted in major economic and environmental ramifications. DNA barcoding is a novel technology that has the potential to improve Integrated Pest Management decision-making, which is dependent on the ability to correctly identify pest and beneficial organisms. This is due to some natural traits such as phenology or pesticide susceptibility browbeaten by IPM strategies to avert pest establishment. Specifically, Deoxyribonucleic acid (DNA) sequence information was applied effectively for the identification of some micro-organisms. This technology, DNA barcoding, allows for the identification of insect species by using short, standardized gene sequences. DNA barcoding is basically based on repeatable and accessible technique that allows for the mechanisation or automation of species discrimination. This technique bridges the taxonomic bio-security gap and meets the International Plant Protection Convention diagnostic standards for insect identification. This review therefore discusses DNA barcoding as a technique for insect pests’ identification and its potential application for crop protection.