<strong>Background:</strong> Placenta extract is used as an alternative medicine especially in Asian countries as it is a rich reservoir of diverse bioactive molecules. The effects of placenta extract on s...<strong>Background:</strong> Placenta extract is used as an alternative medicine especially in Asian countries as it is a rich reservoir of diverse bioactive molecules. The effects of placenta extract on skin conditions have been previously reported, however, the mechanism underlying for reduced wrinkle formation remains unclear. <strong>Objective:</strong> The primary objective of this study was to determine whether the continuous application of porcine placenta extract (PPE) alleviates wrinkle formation in humans and explore the underlying mechanism. <strong>Methods:</strong> Wrinkle formation, skin hydration, and skin elasticity were measured in 15 volunteers at weeks 0 and 6 after continuous application of a gel containing PPE. The production of type I collagen and hyaluronic acid from fibroblasts and keratinocytes, respectively, were measured using ELISA. Expression levels of ceramide synthase 3 (CERS3), filaggrin (FLG), transglutaminase 1 (TGM1), and kallikrein-7 (KLK7) were evaluated by quantitative real-time PCR. <strong>Results:</strong> The wrinkle index was significantly reduced to 72.1% after a 6-week of applying the PPE gel, along with a significant increase in skin hydration to 126.5%. Type I collagen production from fibroblasts was enhanced slightly but significantly following treatment with PPE. PPE accelerated the expression of CERS3 (1.85-fold), FLG (1.35-fold), TGM1 (1.76-fold), and KLK7 (1.62-fold). <strong>Conclusion:</strong> Treatment with PPE alleviates wrinkle formation and simultaneously enhances skin hydration, which is induced via the accelerated expression of moisturizing-related proteins. These findings suggest that PPE is effective for combating dryness-induced wrinkle formation.展开更多
子痫前期(pre-eclampsia,PE)是一种以胎盘及全身系统性炎症为特征的妊娠并发症。PE归类于妊娠期高血压疾病,有进一步发展为子痫的可能,是影响母胎妊娠结局及预后的重要因素,严重影响母婴健康。PE的病理生理机制仍然在进一步的研究中,目...子痫前期(pre-eclampsia,PE)是一种以胎盘及全身系统性炎症为特征的妊娠并发症。PE归类于妊娠期高血压疾病,有进一步发展为子痫的可能,是影响母胎妊娠结局及预后的重要因素,严重影响母婴健康。PE的病理生理机制仍然在进一步的研究中,目前临床上对血压和蛋白尿的检查仍然是识别和诊断PE的重要方式,及时终止妊娠是目前治疗的重要措施。全身系统性的炎症反应是PE发病机制的关键。研究表明,炎症小体NOD样受体家族含pyrin结构域蛋白3(NOD-like receptor family pyrin domain-containing protein 3,NLRP3)是触发级联式炎症反应的“交通枢纽”,是促使炎症因子产生、引起PE炎症反应的“扳机”,NLRP3的激活或抑制与PE的发病及严重程度有关。因此,寻找NLRP3活性改变的原因对于了解PE发病机制及靶向治疗具有重要意义。展开更多
目的分析彩色多普勒超声与血清肌酸激酶(CK)、甲胎蛋白(AFP)相关性在前置胎盘合并产前胎盘植入的诊断价值。方法选择2019年1月至2021年12月在深圳市龙岗区人民医院收治的96例疑似前置胎盘合并产前胎盘植入患者,年龄23~43岁,平均年龄32.3...目的分析彩色多普勒超声与血清肌酸激酶(CK)、甲胎蛋白(AFP)相关性在前置胎盘合并产前胎盘植入的诊断价值。方法选择2019年1月至2021年12月在深圳市龙岗区人民医院收治的96例疑似前置胎盘合并产前胎盘植入患者,年龄23~43岁,平均年龄32.35岁(标准差11.41岁);孕周31~41周,平均孕周35.70周(标准差8.52周)。均行产前彩色多普勒超声检查并分组。行剖宫产,作病理检查。通过酶联免疫吸附分析(ELISA)法检测CK、AFP表达水平。比较两种收缩期峰值血流速度(PSV)水平、搏动指数(PI)、血管阻力指数(RI),分析彩色多普勒超声参数与CK、AFP的相关性。采用受试者工作特性(ROC)曲线分析PSV、PI、RI和三者联合诊断结果比较。结果经彩色多普勒超声检测明确前置胎盘合并产前胎盘植入患者有48例,手术病理检测显示粘连型16例,植入型20例,穿透型12例。依据产前超声图像将其分为48例前置胎盘合并产前胎盘植入患者为研究组,48例非前置胎盘合并产前胎盘植入人员为对照组。两组年龄、孕周差异无统计学意义(P>0.05)。与对照组相比,研究组PSV水平上升,PI、RI下降[(43.23±5.71)cm/s vs(36.74±5.39)cm/s、0.48±0.11 vs 0.68±0.15、0.88±0.31 vs 1.39±0.45。P<0.05]。与粘连型患者相比,植入型患者PSV、CK、AFP水平上升,PI(0.65±0.11 vs 0.77±0.13)、RI(1.24±0.36 vs 1.47±0.59)下降(P<0.05);与植入型患者相比,穿透型患者PSV、CK、AFP水平上升,PI(0.58±0.10 vs 0.65±0.11)、RI(0.80±0.25 vs 1.24±0.36)下降(P<0.05)。PSV、CK、AFP表达水平与不同类型的前置胎盘合并产前胎盘植入呈正相关(r=0.546、0.630、0.547),PI、RI水平与不同类型的前置胎盘合并产前胎盘植入呈负相关(r=-0.481、-0.479)。ROC曲线分析,与PSV、PI、RI单项诊断相比,三项联合诊断对前置胎盘合并产前胎盘植入的诊断价值较高,灵敏度93.80%,特异度91.70%,准确度92.75%(P=0.001)。结论彩色多普勒超声参数在前置胎盘合并产前胎盘植入有着较高的使用价值,且与CK、AFP具有一定的相关性,可用于对前置胎盘合并产前胎盘植入症状变化的评估予以诊治。展开更多
文摘<strong>Background:</strong> Placenta extract is used as an alternative medicine especially in Asian countries as it is a rich reservoir of diverse bioactive molecules. The effects of placenta extract on skin conditions have been previously reported, however, the mechanism underlying for reduced wrinkle formation remains unclear. <strong>Objective:</strong> The primary objective of this study was to determine whether the continuous application of porcine placenta extract (PPE) alleviates wrinkle formation in humans and explore the underlying mechanism. <strong>Methods:</strong> Wrinkle formation, skin hydration, and skin elasticity were measured in 15 volunteers at weeks 0 and 6 after continuous application of a gel containing PPE. The production of type I collagen and hyaluronic acid from fibroblasts and keratinocytes, respectively, were measured using ELISA. Expression levels of ceramide synthase 3 (CERS3), filaggrin (FLG), transglutaminase 1 (TGM1), and kallikrein-7 (KLK7) were evaluated by quantitative real-time PCR. <strong>Results:</strong> The wrinkle index was significantly reduced to 72.1% after a 6-week of applying the PPE gel, along with a significant increase in skin hydration to 126.5%. Type I collagen production from fibroblasts was enhanced slightly but significantly following treatment with PPE. PPE accelerated the expression of CERS3 (1.85-fold), FLG (1.35-fold), TGM1 (1.76-fold), and KLK7 (1.62-fold). <strong>Conclusion:</strong> Treatment with PPE alleviates wrinkle formation and simultaneously enhances skin hydration, which is induced via the accelerated expression of moisturizing-related proteins. These findings suggest that PPE is effective for combating dryness-induced wrinkle formation.
文摘子痫前期(pre-eclampsia,PE)是一种以胎盘及全身系统性炎症为特征的妊娠并发症。PE归类于妊娠期高血压疾病,有进一步发展为子痫的可能,是影响母胎妊娠结局及预后的重要因素,严重影响母婴健康。PE的病理生理机制仍然在进一步的研究中,目前临床上对血压和蛋白尿的检查仍然是识别和诊断PE的重要方式,及时终止妊娠是目前治疗的重要措施。全身系统性的炎症反应是PE发病机制的关键。研究表明,炎症小体NOD样受体家族含pyrin结构域蛋白3(NOD-like receptor family pyrin domain-containing protein 3,NLRP3)是触发级联式炎症反应的“交通枢纽”,是促使炎症因子产生、引起PE炎症反应的“扳机”,NLRP3的激活或抑制与PE的发病及严重程度有关。因此,寻找NLRP3活性改变的原因对于了解PE发病机制及靶向治疗具有重要意义。
文摘目的分析彩色多普勒超声与血清肌酸激酶(CK)、甲胎蛋白(AFP)相关性在前置胎盘合并产前胎盘植入的诊断价值。方法选择2019年1月至2021年12月在深圳市龙岗区人民医院收治的96例疑似前置胎盘合并产前胎盘植入患者,年龄23~43岁,平均年龄32.35岁(标准差11.41岁);孕周31~41周,平均孕周35.70周(标准差8.52周)。均行产前彩色多普勒超声检查并分组。行剖宫产,作病理检查。通过酶联免疫吸附分析(ELISA)法检测CK、AFP表达水平。比较两种收缩期峰值血流速度(PSV)水平、搏动指数(PI)、血管阻力指数(RI),分析彩色多普勒超声参数与CK、AFP的相关性。采用受试者工作特性(ROC)曲线分析PSV、PI、RI和三者联合诊断结果比较。结果经彩色多普勒超声检测明确前置胎盘合并产前胎盘植入患者有48例,手术病理检测显示粘连型16例,植入型20例,穿透型12例。依据产前超声图像将其分为48例前置胎盘合并产前胎盘植入患者为研究组,48例非前置胎盘合并产前胎盘植入人员为对照组。两组年龄、孕周差异无统计学意义(P>0.05)。与对照组相比,研究组PSV水平上升,PI、RI下降[(43.23±5.71)cm/s vs(36.74±5.39)cm/s、0.48±0.11 vs 0.68±0.15、0.88±0.31 vs 1.39±0.45。P<0.05]。与粘连型患者相比,植入型患者PSV、CK、AFP水平上升,PI(0.65±0.11 vs 0.77±0.13)、RI(1.24±0.36 vs 1.47±0.59)下降(P<0.05);与植入型患者相比,穿透型患者PSV、CK、AFP水平上升,PI(0.58±0.10 vs 0.65±0.11)、RI(0.80±0.25 vs 1.24±0.36)下降(P<0.05)。PSV、CK、AFP表达水平与不同类型的前置胎盘合并产前胎盘植入呈正相关(r=0.546、0.630、0.547),PI、RI水平与不同类型的前置胎盘合并产前胎盘植入呈负相关(r=-0.481、-0.479)。ROC曲线分析,与PSV、PI、RI单项诊断相比,三项联合诊断对前置胎盘合并产前胎盘植入的诊断价值较高,灵敏度93.80%,特异度91.70%,准确度92.75%(P=0.001)。结论彩色多普勒超声参数在前置胎盘合并产前胎盘植入有着较高的使用价值,且与CK、AFP具有一定的相关性,可用于对前置胎盘合并产前胎盘植入症状变化的评估予以诊治。
