Effects and Mechanisms of P and K Nutrients on Yield and Protein Content of Fodder Rice

Effects and mechanisms of P and K nutrients on yield and protein content of Weiyou 56, a fodder hybrid rice combination,were studied through pot experiment and biochemical analysis. The results showed that the increase of P and K nutrients enhanced the activities of PEP carboxylase (PEPC), glutamine synthase (GS) and sucrose phosphate synthase (SPS) in leaves, sucrose synthase (SS), ADP glucose pyrophosphorylase (ADPGP) and GS in grains, and the chlorophyll content in leaves, soluble sugar and starch content in grains, protein N and total N content in leaves and grains. Howerer, they decreased soluble sugar content in leaves and led to an increase of protein content in brown rice, biomass, grain yield and harvest index. Excessive P nutrients slightly reduced SPS and ADPG activity in leaves and grains respectively.

Wheat Grain Yield and Protein Content as Influenced by Sodium Bisulfite

Two wheat varieties with similar yield and significantly different protein content were chosen to study the effect of foliage spraying sodium bisulfite (NaHSO3) at low concentration to wheat plants receiving different levels of nitrogen on nitrate reduction along with yield and protein content of grain, so as to discuss the relationship between carbon metabolism and nitrogen metabolism, and find out the intrinsic mechanism of grain yield and protein content formation. The results showed that spraying NaHSO3 at grain-filing stage increased the yield of grain, but declined the capacity of nitrate reduction, which might result in decrease of protein content in the end. Whether receiving NaHSO, or not, the variety with high protein content had higher final protein level and nitrate reduction capacity than that with low protein content.

Effects of early energy and protein restriction on growth performance, clinical blood parameters, carcass yield, and tibia parameters of broilers

financially supported by the Science and Technology Develop Project of Northern Jiangsu, China （BN2015136）;the Priority Academic Program Development of Jiangsu Higher Education Institution, China

Effects of Powdery Mildew on 1 000-Kernel Weight, Crude Protein Content and Yield of Winter Wheat in Three Consecutive Growing Seasons

In order to clarify the impact posed by wheat powdery mildew （Blumeria graminis f. sp. tritici） on the yield and yield components in different epidemic seasons, field trials were conducted in three growing seasons, 2009-2010, 2010-2011 and 2011-2012, in Langfang City, Hebei Province, China. The relationships between 1000-kernel weight, crude protein content of grain and yield and disease index （DI）, as well as area under disease progress curve （AUDPC） were studied. The models of the percentage of loss of 1000-kernel weight, crude protein content and yield were constructed using DI at critical point （CP） of growth stages （GS） and AUDPC in the three growing seasons, respectively. The CPs for estimating 1 000-kernel weight, crude protein content of grain and yield of wheat caused by powdery mildew were GS 11.1, GS 10.5.3 and GS l 0.5.3, respectively. Models based on DI at CP to estimate the percentage of loss of 1000-kernel weight, crude protein content of grain and yield were better than models based on AUDPC. And models of the percentage of loss of 1000-kernel weight, crude protein content and yield for 2011-2012 season were significant different from these for 2009-2010 and 2010-2011 seasons. These results indicated that besides powdery mildew, weather conditions also had influence on 1 000-kernel weight, crude protein content of grain and yield loss of wheat when powdery mildew occurred.

Short-Term Effect of Grazing Exclusion and Uncontrolled Grazing on Species Abundance, Dry Matter Yield and Nutritive Value in an Invaded Area by Euryops floribundus in the Eastern Cape, South Africa

Grazing exclusion (GE) is the most effective rangeland restoration technique which facilitates species diversity and forage quality. This study aimed at assessing short-term impact of GE and continuously grazed rangeland on relative frequency, dry matter yield and nutritive value of dominant grasses in an area invaded by Euryops floribundus. A plot of 2.5 ha was measured and the boundaries demarcated using tape measure and steal pins, the plot was further divided into two subplots of 1ha each which were 5 m apart. One subplot was fenced and protected from grazing livestock, while one subplot was grazed continuously and not fenced. Three parallel belt transects of 100 m × 2 m with 3 m apart were laid out in both subplots. Woody plants occurring within the transects were identified and recorded to determine density. In each subplot, a 0.25 m2 quadrant measuring was thrown randomly to take detailed records on plant species, relative frequency of species and herbage biomass. Four dominant species at the two sites were harvested to determine the nutritive value. Results indicate that grazing exclusion (GE) facilitates grass species diversity, subsequently sixteen and thirteen grasses species were recorded in the GE and uncontrolled grazed (UG) sites, respectively. Eragrostis chloromelas (21.7%), and Themeda triandra (13.2%) had high relative frequencies in the GE site. Highest biomass production was recorded in the GE site (1400 kg·ha-1) compared to UG site (1102 kg·ha-1). Crude protein content was relatively lower at UG site (5.4% - 5.8%) as compared to GE site (7.2% - 7.8%). It was concluded that, GE showed a positive impact on a relative frequency (%), dry matter yield and crude protein content. UG creates a conducive environment for Euryops recruitment. Further studies are required to examine the impact of GE in long-term trial setup.

核桃蛋白提取方法研究进展

核桃粕是核桃榨油后的副产物,富含蛋白质,核桃蛋白中必需氨基酸含量较高,成分均衡,是成人必需氨基酸的良好来源。核桃蛋白传统提取方法主要为碱溶酸沉法,随着提取设备及提取技术的进步,更多的新型方法及组合方式被开发且证实优于传统方法。然而,采用不同的方法提取核桃蛋白的提取率、纯度,以及结构和功能性质存在显著差异。因此本文重点介绍了具有潜在产业化应用价值的核桃蛋白提取方法,包括碱溶酸沉法、超声辅助、酶辅助以及反胶束法,阐述了不同提取方法对核桃蛋白质结构及功能的影响,并对其应用前景进行了展望,旨在为核桃蛋白质资源的深入研究及开发提供参考。

化肥减量配施鱼蛋白有机液肥对番茄产量和品质的影响

为探讨增施鱼蛋白有机液肥替代化肥的效果,以每667m^(2)施用高钾水溶肥5.0kg为对照,设置每667 m^(2)施用高钾水溶肥3.5 kg+鱼蛋白有机液肥5.0 L、高钾水溶肥2.0 kg+鱼蛋白有机液肥5.0 L、高钾水溶肥0.5kg+鱼蛋白有机液肥5.0L这3个化肥减量处理,比较分析化肥减量增施鱼蛋白有机液肥对番茄生长、产量和果实性状及可溶性固形物含量的影响。结果表明:在每667m^(2)施用5.0L鱼蛋白有机液肥的情况下,减少水溶肥用量会降低番茄的株高、叶长和叶宽,但促进了茎粗、果实纵横径的生长,其中不同处理的果实纵径差异不显著,果形指数和可溶性固形物含量随水溶肥用量的减少先升高再降低,以每667 m^(2)每次追施高钾水溶肥3.5 kg和2.0 kg的果形指数最大,但与其他处理没有显著差异。不同处理的平均单穗坐果数没有显著差异,单果质量和果实纵横径的变化趋势一致,每667 m^(2)每次追施高钾水溶肥0.5 kg的小区产量最大。因此,在追求产量的情况下,果实膨大至采收前以每667 m^(2)每次追施高钾水溶肥+0.5 kg鱼蛋白有机液肥5.0 L的肥料配比最适合生产;如果追求品质,每667 m^(2)每次追施高钾水溶肥2.0 kg+鱼蛋白有机液肥5.0 L的肥料配比有利于生产,比对照果实可溶性固形物含量可提高5.8%,667 m^(2)产量提高17.2%。

玉米||豌豆间作体系生产力对覆膜方式及化肥施用量的响应

为研究间作体系生产力对覆膜及化肥施用量的响应,本文基于2012年布设在甘肃省农业科学院张掖节水农业试验站的长期定位试验进行了采样与分析。试验采用三因素试验设计:1)覆膜方式,条膜覆盖和全膜覆盖;2)施肥量,常规施肥[450 kg(N)·hm^(-2), 78.6 kg(P)·hm^(-2)]和减量施肥[300 kg(N)·hm^(-2), 52.4 kg(P)·hm^(-2)];3)种植模式,玉米单作、豌豆单作和玉米||豌豆间作。于2021年和2022年豌豆和玉米收获期采集植株样品,测定籽粒产量、地上部生物量、籽粒粗蛋白产量(基于籽粒氮浓度计算)和吸磷量。结果表明:间作体系产量最高,间作体系混合产量较单作加权产量两年平均提高31.9%,增产主要由正的互补效应驱动;同时,各处理下土地当量比均大于1,即间作能够提高土地利用率。相比于单作,间作显著增加籽粒粗蛋白产量和磷吸收量,两年平均分别提高30.0%和24.0%。与条膜覆盖相比,全膜覆盖显著提高生产力和粗蛋白产量。其中,在条膜覆盖下,间作较单作增产38.4%;全膜覆盖下,间作较单作增产26.3%;单作和间作条件下,全膜较条膜分别增产15.9%和5.8%;与单作覆条膜相比,间作覆全膜的产量显著提高47.0%,粗蛋白产量显著提高57.5%。两个施肥水平产量无显著差异,与常规施肥的单作相比,减量施肥下的间作氮肥和磷肥偏生产力均提高102.0%,具有更高的资源利用效率。在减量施氮肥和磷肥条件下,间作相比于单作提高了肥料偏生产力和氮磷收获指数,且玉米品质不降低。因此,与农户模式(条膜覆盖下常规施肥的玉米单作)相比,全膜覆盖下减量施肥的间作是一种更加可持续发展的种植模式,是农业绿色发展的重要措施。

蛋白质谷氨酰胺酶在乳清蛋白肽酶法制备中的应用

乳清蛋白是公认的人体优质蛋白质补充剂,却存在着一些过敏蛋白,为了降低乳清浓缩蛋白中的过敏原含量,开发β-乳球蛋白(β-lactoglobulin,β-LG)抗原性较低的乳清蛋白肽,该研究建立基于蛋白酶与蛋白质谷氨酰胺酶(protein glutaminase,PG)的乳清蛋白肽酶解工艺。通过蛋白酶筛选与复配确定最佳酶解组合——碱性蛋白酶+Prote AXH,建立PG辅助蛋白酶酶解工艺:5%乳清蛋白溶液在50℃下,加入1%PG充分孵育0.5 h后调整pH值为7.5,分步加入1%碱性蛋白酶和1%Prote AXH共反应2 h,最终得到的深度水解乳清蛋白粉水解度可达29.82%,分子质量<3000 Da的占比为88.48%,得率在93.5%,β-LG的降解率可达97.5%,必需氨基酸比例在46.16%。综上所述,PG的添加对提高乳清蛋白肽得率和过敏原降解率均有明显的效果,该研究可为低致敏乳清蛋白肽酶法制备提供一定的技术参考。

高蛋白高产大豆新品种吉育2517的选育研究

高蛋白高产大豆新品种吉育2517是由吉林省农业科学院大豆研究所以吉育257为母本、金源71为父本配制杂交组合,采用单粒传法经多年鉴定选育而成。2020—2021年参加吉林省大豆科企联合体中早熟组区域试验平均产量2950.4 kg·hm^(-2),较对照品种合交02-69平均增产5.1%。2022年参加生产试验,平均产量3183.9 kg·hm^(-2),较对照品种合交02-69平均增产3.7%。该品种丰产性和稳产性较好,蛋白质含量两年平均为43.13%,属高蛋白品种。2023年通过吉林省农作物品种审定委员会审定。同时对吉育2517适宜的栽培模式进行了探索,并在2022—2023年进行良种良法配套生产示范,两年平均产量3041.9 kg·hm^(-2),较对照增产6.6%。

近20年来中国小麦品种产量和品质性状变化分析

高产优质是中国小麦生产发展方向,及时总结中国小麦育种进展对明确小麦产量和品质能否协同提高至关重要,也可为未来小麦育种提供借鉴。本研究对1999-2020年中国审定的646个小麦品种的产量和品质进行了整理与分析。结果表明,1999-2020年,中国春麦区、黄淮冬麦区和长江冬麦区的小麦产量显著上升,年均增长率分别为2.58%、 1.13%和1.49%;而北部冬麦区小麦产量变化不明显。各区域平均产量以黄淮冬麦区最高(7 606 kg·hm^(-2)),春麦区最低(4 669 kg·hm^(-2))。中国春麦区和长江冬麦区小麦产量提高主要是由于穗数和穗粒数显著增加,而黄淮冬麦区小麦产量提高主要是由于穗数和千粒重显著增加。小麦籽粒蛋白质含量在春麦区显著降低,且与产量呈极显著负相关;其余区域蛋白质含量均无显著变化,且与产量均无显著相关性。各区域湿面筋含量与蛋白质含量均呈显著正相关,但与产量相关不显著。

施用有机肥和秸秆还田对旱地玉-麦二熟体系作物产量、品质和化肥效率的影响

为探明施用商品有机肥和秸秆还田对旱地夏玉米-冬小麦二熟体系作物产量、品质和肥料利用效率的影响,于2015—2020年度设置不施肥+秸秆不还田(CK)、施氮磷肥+秸秆不还田(NP)、施氮磷肥+秸秆不还田+商品有机肥(NPO)和施氮磷肥+秸秆还田(NPS)4个处理进行田间试验,测定并分析作物产量、氮磷肥农学效率、籽粒养分含量、蛋白质含量以及冬小麦籽粒中蛋白质组分含量。结果表明:(1)与NP和NPO处理相比,NPS处理夏玉米5 a平均产量分别显著提高21.33%和20.77%,周年产量分别显著提高9.99%和13.43%,但3个施肥处理间小麦产量差异不显著。(2)施用商品有机肥和秸秆还田对夏玉米、冬小麦的籽粒品质均有显著影响。与NP处理相比,NPO处理冬小麦籽粒各蛋白质组分含量提高5.69%~8.28%;NPS处理冬小麦籽粒氮含量、钾含量和各蛋白质组分含量分别提高4.68%、3.96%和7.75%~11.38%。相较于NPO处理,NPS处理冬小麦籽粒钾含量、谷蛋白含量、贮藏蛋白含量和谷醇比分别显著提高14.41%、9.86%、5.39%和9.65%,夏玉米籽粒蛋白质含量、产量和籽粒氮、籽粒磷含量以及周年蛋白质产量分别显著提高6.45%、28.89%、6.40%、23.08%和10.43%。(3)相较于NP和NPO处理,NPS处理夏玉米氮肥的农学效率分别提高118.35%和113.25%,周年氮、磷肥农学效率分别提高42.83%和64.36%、42.86%和64.27%,但3个施肥处理间冬小麦氮肥、磷肥农学效率5 a均值无显著差异。综合来看,长期施用商品有机肥和秸秆还田可改善冬小麦籽粒中的蛋白质组分特性,秸秆还田还能增加夏玉米籽粒钾含量和冬小麦籽粒蛋白质含量和蛋白质产量,秸秆还田较施用商品有机肥更利于提高旱地玉-麦二熟区作物产量、品质和肥料利用效率。综上所述,施氮磷肥+秸秆还田的管理模式更适宜在旱地玉-麦二熟区进行推广。

不同乳产量、乳蛋白率双峰驼血液生化指标和激素水平的对比研究

为了研究不同乳产量和乳蛋白率泌乳双峰驼血液生化指标和激素含量的差异,选用24峰体况、胎次相近的处于泌乳初期的阿拉善双峰驼,分为高产乳量组、低产乳量组、高乳蛋白组、低乳蛋白组,测定其血液指标,包括血清生化指标和血清激素水平。结果显示,在乳产量分组中,高产组双峰驼钙离子和钾离子显著低于低产组(P<0.05),非酯化脂肪酸极显著低于低产组(P<0.01),血清胰岛素浓度显著高于低产组(P<0.05);在乳蛋白率分组中,高乳蛋白组的血尿素氮浓度极显著高于低乳蛋白组(P<0.01),高乳蛋白组的孕酮和雌三醇显著低于低乳蛋白组(P<0.05),催乳素、雌酮和瘦素极显著低于低乳蛋白组(P<0.01)。结果表明,在相同的饲养管理下,离子代谢和糖代谢对乳产量的差异起到重要作用,乳蛋白的合成效率的差异可能主要由激素调节。

施氮量对强筋小麦光合、产量、蛋白质含量和加工品质的影响

为探究冀东地区强筋小麦适宜施氮量,以强筋小麦石优20号和中麦998为材料,通过大田试验设置0 kg·hm^(-2)(N_(0))、180 kg·hm^(-2)(N 180)、210 kg·hm^(-2)(N 210)和240 kg·hm^(-2)(N 240)4个施氮水平,分析了施氮量对强筋小麦光合、产量、蛋白质含量和加工品质的影响。结果表明,与N_(0)处理相比,施氮后石优20号和中麦998的产量均在N 240处理下增加显著,增幅分别为3.20%和13.95%。随施氮量的增加,小麦旗叶叶绿素含量和光合参数均呈先升后降趋势,且在花后15 d达到最大值。施氮对F v/F m影响较小,对ΦPSⅡ影响较明显。旗叶、籽粒内硝酸还原酶和谷氨酰胺合成酶均随施氮量的增加呈先升后降趋势。增施氮肥后籽粒蛋白组分中清蛋白、球蛋白比例下降,醇溶蛋白、谷蛋白比例上升,各组分含量在N 210处理下均最高。面筋含量、沉降值、形成时间和稳定时间均随施氮量的增加先升后降。这说明增施氮肥可改善強筋小麦植株的光合特性,进而提高产量,同时增加植株体内氮代谢相关酶活性和籽粒蛋白质含量并改变蛋白质组分所占比例,有效改善加工品质。本试验条件下,210 kg·hm^(-2)施氮量下,小麦产量和品质较优,该施氮量可作为冀东平原强筋小麦栽培的最佳施氮量。

蚕豆蛋白的分离提取方法

蚕豆富含蛋白质、淀粉和脂肪等多种营养成分,营养价值丰富,是我国重要的植物资源。本文结合国内外相关领域的研究文献,阐述蚕豆蛋白的分离提取方法,旨在为提高蚕豆蛋白提取率及蚕豆深加工水平提供一定的理论支持。

暖季补饲对高寒牧区天然草地放牧泌乳牦牛产乳性能的效应

青藏高原高寒草甸草地牧草营养品质虽高,但产量极低,尤其缺乏蛋白饲草且草地呈现不同程度退化,导致泌乳牦牛(Bos grunniens)营养不良,乳产量和品质呈下降趋势。另外,多汁饲料因富含维生素、水分等,通常作为提高奶牛产乳性能的重要饲料种类,但其对泌乳牦牛产乳性能的影响尚不明确。由于青藏高原牧区大多相对干旱,天然草地植物水分含量相对较少,因此预测补给多汁饲料,可能也会提高泌乳期牦牛维生素以及水分的摄取,提高泌乳性能。本研究于2021年暖季(7月-10月)在西藏那曲高寒牧区筛选了体重、年龄、胎次相近的带犊泌乳牦牛24头,随机分4组,在天然草地传统放牧基础上分别设蛋白质饲料[苜蓿(Medicago sativa):1 kg·(d·head)^(-1)]、多汁饲料[萝卜(Raphanus sativus):1 kg·(d·head)^(-1)]、蛋白质加多汁饲料[萝卜和苜蓿各0.5 kg·(d·head)^(-1)]补给和不补给饲料(对照)处理,探讨暖季补给蛋白质和多汁饲料对青藏高原高寒牧区天然草地放牧泌乳期牦牛乳产量和品质(乳脂率、蛋白质率、非脂乳固体率、乳糖率、灰分率、密度、冰点)的影响。结果表明:与对照比较,蛋白质饲料补给显著提高了天然草地放牧泌乳牦牛日产乳量(P<0.05),提高率达22%,延缓了泌乳期牦牛产乳量随时间下降的趋势;多汁饲料补给显著降低了天然草地放牧泌乳牦牛日产乳量,降低率为6%(P<0.05)。与对照比较,单独补给蛋白质饲料或蛋白质加多汁饲料均显著提高了天然草地放牧泌乳牦牛的乳蛋白质率、非脂肪固体率、乳糖率、乳灰分以及乳密度,显著降低了乳冰点,同时补给蛋白质加多汁饲料显著降低了乳脂率。另外,与对照比较,仅补给多汁饲料显著提高了乳脂率、蛋白质率、非脂肪固体率和灰分率,显著降低了乳冰点。但3种补饲处理对泌乳牦牛和犊牛体重均无显著影响(P>0.05)。综上,即使在牧草生长旺盛的暖季,天然草地仍无法满足泌乳牦牛营养需求。且补给蛋白质和多汁饲料均有效提高泌乳牦牛产乳潜能。因此对缓解草畜矛盾,加速当地畜牧业发展以改善当地生活状况具有重要意义。

施肥和豆禾混播对松嫩平原退化草地修复的影响

【目的】探索适合松嫩平原中度退化草地的最佳修复模式,为松嫩平原退化草地的保护修复提供技术支持。【方法】设置紫花苜蓿(Medicago sativa)与羊草(Leymus chinensis)2种混播比例,结合不同N施用量,分析土壤养分含量、牧草品质和产量之间的关系。【结果】施肥水平显著影响群落物种丰富度(P<0.01),施肥水平、混播比例及二者交互显著影响土壤有机质、Shannon⁃Wiener多样性指数、牧草粗蛋白含量及产量(P<0.05)。土壤全氮、牧草粗蛋白含量与施氮量存在极显著正相关性(P<0.01)。两种混播比例下,土壤全氮含量变化与N施用量一致;N施用量300 kg/hm2的牧草粗蛋白含量高达20.22%,与其他处理差异显著(P<0.05)。【结论】N施用量200~250 kg/hm2在试验当年秋季和第二年春季均获得最佳产量;豆禾1∶2混播的群落丰富度和多样性优于2∶2混播,其中N施用量150 kg/hm2表现最佳。

微波预处理对花生压榨特性的影响

采用微波对花生进行预处理,研究处理前后花生水分、褐变程度、颗粒质构参量、水分状态分布、蛋白变性程度的变化趋势以及对压榨出油率的影响,并与真空烘箱处理作对比,经Pearson相关性分析,建立各指标与出油率之间的数学相关性,为花生的高效加工利用提供理论依据和数据参考。结果表明,花生在900 W条件下微波7 min,或者85℃真空烘箱烘制89 min,水分质量分数从5.25%降至2.27%,此时压榨出油率最高,分别为45.85%和45.76%;此条件下,花生与榨油机之间构成的切应力适中,花生受到充分挤压,发生形变,油脂大量流出,且花生可塑性最好,水分状态组成最为合理,蛋白变性程度适中,既可以使得油脂大量流出,又不会轻易破碎影响饼粕成形。

川西北高寒地区5种饲草产量与CNCPS组分分析

饲草种质资源是新品种创制的基础,本研究选择适宜在川西北高寒区栽培的中华羊茅(Festuca sinensis)、无芒雀麦(Bromus inermis)、垂穗披碱草(Elymus nutans)、圆柱披碱草(Elymus cylindricus)和老芒麦(Elymus sibiricus)饲草种质资源,测定了饲草产量、全株及不同部位(茎、叶、穗)的营养成分,利用康奈尔净碳水化合物-蛋白质体系(Cornell net carbohydrate and protein system, CNCPS)对其组分进行分析,为筛选高产优质饲草种质资源奠定基础。结果表明,鲜草产量随年份增加逐年降低,老芒麦连续四年的鲜草产量高于其他饲草。圆柱披碱草的干草产量最高,变异系数较小,稳产性较好。无芒雀麦茎、叶和穗的中性洗涤纤维(Neutral detergent fiber, NDF)、酸性洗涤纤维(Acid detergent fiber, ADF)含量低于其他饲草,非结构性碳水化合物(Nonstructural carbohydrate, NSC)含量显著高于其他饲草(P<0.05)。圆柱披碱草茎、叶和穗的ADF含量显著高于其他饲草(P<0.05)。老芒麦全株、茎、叶和穗的粗蛋白(Crude protein, CP)含量高于其他饲草,碳水化合物(Carbohydrate, CHO)含量低于其他饲草。综合分析得出,无芒雀麦和老芒麦的生产性能及营养品质表现较好,可作为当地种质资源研究和新品种选育的基础材料。

Individual and interactive influences of elevated air temperature and soil drought at the flowering and boll-forming stage on cottonseed yield and nutritional quality

As the main byproduct of cotton production,cottonseed yields edible vegetable oil,ruminant feed,and industrial products.We evaluated the individual and interactive effects of elevated air temperature and soil drought on cottonseed yield and nutritional quality using two cotton cultivars,Sumian 15(heat-susceptible)and PHY370 WR(heat-tolerant).The experiment was conducted under three levels of soil relative water content(SRWC):(75±5)%,(60±5)%and(45±5)%and two temperature regimes:ambient temperature(AT,31.0/26.4℃,mean daytime/night temperature)and elevated temperature(ET,33.4/28.9℃).Cottonseed yield,boll number,seed number,and single-seed weight were lower under combined ET and SRWC(45±5)%than either individual stress or combined stresses in comparison with the control treatment(SRWC(75±5)%under AT).Drought tended to increase oil content and reduce protein content,whereas ET showed almost the opposite effects.Under the combination of ET and soil drought,oil content was still higher than under control,although ET weakened the beneficial effects of drought.For protein,ET offset the negative impacts of mild drought on protein content,but protein content was not increased under SRWC(45±5)%.Each stress or combined stress reduced oil and protein yields under all treatments,owing to declines in cottonseed yields.The combined stress reduced unsaturated fatty acid(UFA)/saturated fatty acid(SFA)and essential amino acid(EAA)/non-essential amino acid(NAA).Compared with PHY370 WR,the sensitivity of Sumian 15 to the combined factors was evidenced in the following ways:(1)seed yield,yield components,oil and protein yields were decreased more for Sumian 15 than PHY370 WR compared with the control treatment;(2)the combined stresses caused lower oil content,UFA,and UFA/SFA in Sumian 15 than PHY370 WR;(3)interaction effects of the combined factors on protein content and EAA/NAA were detected only in Sumian 15.