Comparative Analysis of Ki-67 Protein as a Proliferative Expression Index in Cutaneous Basal and Squamous Cell Carcinoma in Federal Medical Centre Umuahia, Nigeria

Background: Evaluating the tumor proliferative index helps predict clinical behavior and provides prognostic insights for cutaneous basal cell carcinoma (cBCC) and squamous cell carcinoma (cSCC). Objective: This study aimed to identify differences in the proliferative indices among variants of cBCC and cSCC diagnosed at a tertiary healthcare center. Method: Skin biopsies histologically diagnosed as cBCC and cSCC between 2012 and 2018 at the Federal Medical Centre (FMC) Umuahia, Abia State, Nigeria, were analyzed. Archival formalin-fixed, paraffin-embedded (FFPE) tissue blocks were retrieved along with clinical data, and were prepared on charged microscope slides and the immunohistochemical staining was carried out. The primary antibody used in this study was clone BioCare CRM325C (RM) and adenotonsillar tissue blocks/slides served as positive controls. Ki-67 immunohistochemistry was performed on fresh 4µm sections of the tumor specimens. Results: The application of Ki-67 immunoperoxidase on both BCC and SCC cohort, yielded an intense observable brownish nuclear stain in areas of dense proliferating tumour cells on both cutaneous tumours. The average Ki-67 index for all cSCC cases was 24.7%, with a range of 2.3% - 80%, while the mean for cBCC was 15.8%, ranging from 1.2% - 45.6%. Variants with high proliferative indices were observed in 11.9% of cBCC cases and 29.1% of cSCC cases. Among the low proliferative index category, cSCC accounted for 5.4%, while cBCC represented 14.3%. For mild proliferative indices, cSCC cases made up 7.3% and cBCC, 11.9%. The majority of cases showed moderate proliferative indices, with 61.9% for cBCC and 58.2% for cSCC. Overall, there was a significant difference in proliferative indices between cSCC, cBCC, and their variants. Conclusion: The study found a significantly higher rate of cell proliferation, measured by Ki-67 immunostaining, in cSCC and its variants compared to cBCC. However, certain variants of cBCC also exhibited high Ki-67 expression, indicating they can be as aggressive as some cSCC variants.

Influence of norcantharidin on proliferation,proliferation-related gene proteins prolifera-ting cell nuclear antigen and Ki-67 of human gallbladder carcinoma GBC-SD cells

BACKGROUND: Gallbladder carcinoma is a highly lethal and aggressive disease with early metastasis, strong invasion and poor prognosis. Most patients with this disease are at the advanced and un-resectable stage and should be consi- dered for palliative treatment such as chemotherapy and ra- diotherapy. Unfortunately, reports of chemotherapy and radiotherapy for gallbladder carcinoma are disappointing. We investigated the influence of norcantharidin (NCTD) on proliferation, proliferation-related gene proteins PCNA and Ki-67 of human gallbladder carcinoma GBC-SD cells in vitro. METHODS: GBC-SD cell lines of human gallbladder carci- noma were cultured by the cell culture technique. The ex- periment was divided into NCTD group and control group. The tetrazolium-based colorimetric assay was used to evaluate cell growth. The streptavidin-biotin complex method was used to determine the expressions of prolifera- tion-related gene proteins PCNA and Ki-67 of human gall- bladder carcinoma GBC-SD cells. RESULTS: NCTD inhibited the growth and proliferation of GBC-SD cells from 10 mg/L or after 6 hours in a dose- and time-dependent manner, with the IC50 value of 56.18 μg/ ml at 48 hours. After treatment with NCTD, the expression of PCNA (0.932 ±0.031 vs. 0.318 ±0.023, P<0.001) and Ki-67 (0.964 ±0.092 vs. 0.297 ±0.018, P<0.001) proteins were decreased significantly. CONCLUSION: NCTD inhibits the proliferation of human gallbladder carcinoma GBC-SD cells in vitro and the expres- sion of their proliferation-related gene proteins PCNA and Ki-67.

Overexpression of carbonic anhydrase Ⅱ and Ki-67 proteins in prognosis of gastrointestinal stromal tumors

AIM: To investigate the expression and prognostic value of carbonic anhydrase Ⅱ (CA Ⅱ) and Ki-67 in gastrointestinal stromal tumors (GISTs). METHODS: One hundred and thirteen GIST patients admitted to Chinese People's Liberation Army General Hospital from January 2004 to December 2010 were retrospectively followed up, and immunohistochemistry was used to detect CA Ⅱ, Ki-67 and CD117 expression in tumor samples. The survival rates of the patients were analyzed using the Kaplan-Meier method. Log-rank test, χ 2 test and Cox proportional hazards model were used to determine the relationships between CA Ⅱ, Ki-67 and CD117 expression and prognostic value in GISTs. RESULTS: The survival rates at 1, 3 and 5 years were 90.0%, 82.0% and 72.0% in all patients. However, in patients with positive CA Ⅱ or Ki-67, the survival rates were 92.0%, 83.0% and 77.0% or 83.0%, 66.6% and 53.0%, respectively. Compared with the negative groups, the survival rates in the positive groups were significantly lower (CA Ⅱ log-rankP = 0.000; Ki-67 logrank P = 0.004). Multivariate Cox analysis revealed that CA Ⅱ, CD117 and Ki-67 were considerable immune factors in prognosis of GIST patients (CA Ⅱ P = 0.043; CD117 P = 0.042; Ki-67 P = 0.007). Besides, tumor diameter, mitotic rate, tumor site, depth of invasion, complete resection, intraoperative rupture, and adjuvant therapy were important prognosis predictive factors. Our study indicated that CA Ⅱ had strong expression in GISTs and the prognosis of GISTs with high CA Ⅱ expression was better than that of GISTs with low or no expression, suggesting that CA Ⅱ is both a diagnostic and prognostic biomarker for GIST. CONCLUSION: CA Ⅱ and Ki-67 are significant prognostic factors for GISTs. CA Ⅱ associated with neovascular endothelia could serve as a potential target for cancer therapy.

Marker Ki-67 is a potential biomarker for the diagnosis and prognosis of prostate cancer based on two cohorts

BACKGROUND Prostate cancer(PCa)is a widespread malignancy,predominantly affecting elderly males,and current methods for diagnosis and treatment of this disease continue to fall short.The marker Ki-67(MKI67)has been previously demonstrated to correlate with the proliferation and metastasis of various cancer cells,including those of PCa.Hence,verifying the association between MKI67 and the diagnosis and prognosis of PCa,using bioinformatics databases and clinical data analysis,carries significant clinical implications.AIM To explore the diagnostic and prognostic efficacy of antigens identified by MKI67 expression in PCa.METHODS For cohort 1,the efficacy of MKI67 diagnosis was evaluated using data from The Cancer Genome Atlas(TCGA)and Genotype-Tissue Expression(GTEx)databases.For cohort 2,the diagnostic and prognostic power of MKI67 expression was further validated using data from 271 patients with clinical PCa.RESULTS In cohort 1,MKI67 expression was correlated with prostate-specific antigen(PSA),Gleason Score,T stage,and N stage.The receiver operating characteristic(ROC)curve showed a strong diagnostic ability,and the Kaplan-Meier method demonstrated that MKI67 expression was negatively associated with the progression-free interval(PFI).The time-ROC curve displayed a weak prognostic capability for MKI67 expression in PCa.In cohort 2,MKI67 expression was significantly related to the Gleason Score,T stage,and N stage;however,it was negatively associated with the PFI.The time-ROC curve revealed the stronger prognostic capability of MKI67 in patients with PCa.Multivariate COX regression analysis was performed to select risk factors,including PSA level,N stage,and MKI67 expression.A nomogram was established to predict the 3-year PFI.CONCLUSION MKI67 expression was positively associated with the Gleason Score,T stage,and N stage and showed a strong diagnostic and prognostic ability in PCa.

Fluctuation of Microfibrillar Protein Level in Lutoids of Primary Laticifers in Relation to the 67 kD Storage Protein in Hevea brasiliensis

Abundant microfibrillar protein inclusions were present in the lutoids of the primary laticifers in Hevea brasiliensis Mull. Arg. Two forms of the inclusions could be distinguished under the electron microscope, each in separate lutoids. As revealed by SDS-PAGE, the 59.5 kD and 63.5 kD proteins were the major components of the microfibrillar protein purified by isoeleettic point precipitation. Western-blotting analysis indicated that they were immunorelated with the 67 kD protein accumulated in the protein-storing cells. The 59.5 kD and 63.5 kD proteins were abundant in the uppermost part, the stem of new shoot and sustained their abundance during the growth and development of new shoot while their contents decreased remarkably in the lower parts of the trunk, accompanying by the accumulation of 3-5 kinds of proteins with low molecular weights. This fluctuating pattern suggested that the degradation of the 59.5 kD and 63.5 kD proteins had nothing to do with the new shoot growth and may be closely related to the primary laticifer differentiation. The 67 kD protein could not be detected in the young stem of new shoot when its leaves were broze-colored while the protein started to be accumulated in the stem, when the leaves of new shoot had matured, behaving like a typical vegetative storage protein.

Prognostic significance of grade of malignancy based on histopathological differentiation and Ki-67 in pancreatic ductal adenocarcinoma

Objective:Tumor cell malignancy is indicated by histopathological differentiation and cell proliferation.Ki-67,an indicator of cellular proliferation,has been used for tumor grading and classification in breast cancer and neuroendocrine tumors.However,its prognostic significance in pancreatic ductal adenocarcinoma(PDAC)remains uncertain.Methods:Patients who underwent radical pancreatectomy for PDAC were retrospectively enrolled,and relevant prognostic factors were examined.Grade of malignancy(GOM),a novel index based on histopathological differentiation and Ki-67,is proposed,and its clinical significance was evaluated.Results:The optimal threshold for Ki-67 was determined to be 30%.Patients with a Ki-67 expression level>30%rather than≤30%had significantly shorter 5-year overall survival(OS)and recurrence-free survival(RFS).In multivariate analysis,both histopathological differentiation and Ki-67 were identified as independent prognostic factors for OS and RFS.The GOM was used to independently stratify OS and RFS into 3 tiers,regardless of TNM stage and other established prognostic factors.The tumor-nodemetastasis-GOM stage was used to stratify survival into 5 distinct tiers,and surpassed the predictive performance of TNM stage for OS and RFS.Conclusions:Ki-67 is a valuable prognostic indicator for PDAC.Inclusion of the GOM in the TNM staging system may potentially enhance prognostic accuracy for PDAC.

Correlation of dynamic contrast-enhanced ultrasonography and the Ki-67 labelling index in pancreatic ductal adenocarcinoma

BACKGROUND Pancreatic ductal adenocarcinoma(PDAC)is a highly malignant and aggressive tumor,and high Ki-67 expression indicates poor histological differentiation and prognosis.Therefore,one of the challenges in diagnosing preoperatively patients with PDAC is predicting the degree of malignancy.Dynamic contrast-enhanced ultrasonography(DCE-US)plays a crucial role in abdominal tumor diagnosis,and can adequately show the microvascular composition within the tumors.However,the relationship between DCE-US and the Ki-67 labelling index remains unclear at the present time.AIM To predict the correlation between Ki-67 expression and the parameters of DCEUS.METHODS Patients with PDAC who underwent DCE-US were retrospectively analyzed.Patients who had received any treatment(radiotherapy or chemotherapy)prior to DCE-US;had incomplete clinical,imaging,or pathologic information;and had poor-quality image analysis were excluded.Correlations between Ki-67 expression and the parameters of DCE-US in patients with PDAC were assessed using Spearman’s rank correlation analysis.The diagnostic performances of these parameters in high Ki-67 expression group were evaluated according to receiver operating characteristic curve.RESULTS Based on the Ki-67 labelling index,30 patients were divided into two groups,i.e.,the high expression group and the low expression group.Among the relative quantitative parameters between the two groups,relative half-decrease time(rHDT),relative peak enhancement,relative wash-in perfusion index and relative wash-in rate were significantly different between two groups(P=0.018,P=0.025,P=0.028,P=0.035,respectively).The DCE-US parameter rHDT was moderately correlated with Ki-67 expression,and rHDT≥1.07 was more helpful in accurately diagnosing high Ki-67 expression,exhibiting a sensitivity and specificity of 53.8%and 94.1%,respectively.CONCLUSION One parameter of DCE-US,rHDT,correlates with high Ki-67 expression.It demonstrates that parameters obtained noninvasively by DCE-US could better predict Ki-67 expression in PDAC preoperatively.

Galectin-3 VEGF Ki-67 MVD在青年乳腺癌淋巴结转移灶中的表达及意义

目的:探讨青年乳腺癌淋巴结转移灶Galectin-3、VEGF、Ki-67、MVD的表达及意义。方法:应用免疫组化SP法检测35例青年乳腺癌淋巴结转移灶(青年组)和35例其它年龄组乳腺癌淋巴结转移灶(对照组)Galectin-3、VEGF、Ki-67、MVD的表达情况。结果:Galectin-3在青年组的表达水平明显高于对照组(P<0.01),而VEGF的表达水平在两组之间无明显差异(P>0.05);青年组Ki-67和MVD明显高于对照组,差异有极显著性(P<0.01和P<0.01)。Galectin-3表达与Ki-67和MVD呈显著正相关(P<0.01和P<0.01)。结论:Galectin-3可能参与了瘤细胞的增殖和血管形成;青年乳腺癌淋巴结转移灶的癌细胞有更强的增殖、浸润和血管形成能力,这是青年乳腺癌恶性度高,预后差的重要因素。

骨肉瘤CD133 CD117 Ki-67的表达及与临床病理因素和危险度的相关性研究

目的:研究骨肉瘤组织CD133、CD117及Ki-67在蛋白水平的表达情况及其与临床病理因素和危险度的关系。方法:应用免疫组织化学PV-9000二步法检测55例骨肉瘤组织标本中CD133、CD117和Ki-67的表达,并与临床病理学指标和术后无瘤生存期进行比较分析,对照组为20例骨软骨瘤。应用SPSS 17.0软件统计分析,检验标准为P<0.05具有统计学意义。结果:骨肉瘤组织CD133、CD117、Ki-67蛋白表达阳性率显著高于良性的骨软骨瘤组织,差异有统计学意义(分别为P=0.016、P=0.008、P<0.001);CD133或Ki-67蛋白阳性表达骨肉瘤患者平均生存时间及平均转移时间短于CD133或Ki-67蛋白阴性骨肉瘤患者,差异有统计学意义(P<0.05);CD133、Ki-67在外科分期和远处转移对骨肉瘤患者预后有影响,其中CD133是外科分期及远处转移而影响骨肉瘤患者预后的独立因素。结论:CD133和Ki-67表达可能在骨肉瘤发生、发展过程中起重要作用,有望成为判断其预后的指标。

胃安宁颗粒对肿瘤血管VEGF Ki-67表达影响的实验研究

目的:观察胃安宁颗粒对SGC-7901荷瘤裸鼠血管VEGF、Ki-67表达的影响。方法:将裸鼠皮下接种胃癌SGC-7901细胞后随机分为对照组、阳性药组及胃安宁颗粒大、中、小剂量组,用药后分别计算瘤重系数,并将肿瘤组织进行免疫组织化学染色,检测VEGF、Ki-67表达的情况。结果:胃安宁颗粒大、中剂量均能明显降低荷瘤裸鼠瘤重和瘤重系数(P<0.05～0.001),免疫组织化学染色显示,应用胃安宁颗粒后肿瘤组织VEGF、Ki-67的表达有所下降。结论:胃安宁颗粒可能通过抑制VEGF、Ki-67的表达起到抑制肿瘤血管生成的作用,从而达到抗肿瘤的目的。

VEGF Ki-67在前列腺癌组织中的表达及其与MVD的相关性研究

目的:探讨VEGP、Ki-67在前列腺癌组织中的表达及其与肿瘤微血管密度(Microvessel density,MVD)计数的关系。方法:采用免疫组织化学SP法检测50例前列腺癌和10例良性前列腺增生石蜡包埋组织中VEGF、Ki-67的表达和MVD计数。结果:前列腺癌中VEGF阳性表达率为80%,Ki-67阳性表达率为90%,MVD计数为20.80±10.47;良性前列腺增生中VEGF阳性表达率为30%,Ki-67阳性表达率为0,MVD计数为3.93±2.33。两组比较均有统计学意义(P<0.05),且前列腺癌的分化程度越低,其VEGF、Ki-67的阳性表达率越高,MVD计数也越高。前列腺癌VEGF和Ki-67的阳性表达率与MVD计数呈正相关(P<0.01)。结论:VEGF、Ki-67和MVD在前列腺癌的发生发展中具有协同作用,联合检测有助于判断其生物学行为和预后。

TGF-α Ki-67与脑膜瘤良恶性及侵袭性的相关研究

目的 探讨转化生长因子α (TGF α)和Ki-67在脑膜瘤组织中的表达及其与肿瘤增殖潜力 ,良恶性及侵袭性的关系。方法 采用免疫组化二步法 ,检测TGF α、Ki-67在 5 7例脑膜瘤组织中的表达。结果 良性、非典型性、恶性脑膜瘤中TGF α分数分别为 2 0± 1 2 4、 2 8± 0 84、 3 85± 1 0 7。恶性脑膜瘤TGF -a分数显著高于良性脑膜瘤 (P <0 0 5 )。良性、非典型性、恶性脑膜瘤中MIB— 1L1(Ki -67)分别为 0 879±0 5 4、 3 84± 0 94、 7 85± 3 19。恶性脑膜瘤平均MIB— 1L1(Ki-67)明显高于非典型性 (P <0 0 5 )和良性脑膜瘤 (P <0 0 5 )。TGF α表达与Ki-67的表达呈正相关 (r=0 3 2 1)。结论 脑膜瘤TGF α ,Ki-67表达与其良恶性有关 ,检测脑膜瘤中TGF α 。

缺氧诱导因子-1α Ki-67在前列腺癌中的表达及意义

目的探讨前列腺癌中缺氧诱导因子(HIF)-1α、Ki-67表达的相互关系及临床意义。方法采用免疫组织化学法检测HIF-1α、Ki-67在前列腺增生和前列腺癌组织中的表达。结果前列腺癌组织中HIF-1α与Ki-67的阳性表达率分别为69%和64%,并且与前列腺癌的组织学分级、临床分期呈正相关(P<0.05)。HIF-1α与Ki-67表达密切相关(rs=0.362,P<0.01)。结论HIF-1α过度表达可能参与前列腺癌的发生发展,且与前列腺癌细胞增殖有密切关系,可作为反映前列腺癌生物学行为的有效指标。

中期因子CD105 Ki-67在胆囊癌的表达及临床意义

目的通过观察中期因子(MK)蛋白在胆囊癌中的表达,结合临床病理资料,探讨中期因子蛋白、CD105及Ki-67抗原表达与胆囊癌血管生成、增殖活性、侵袭和转移的关系。方法采用免疫组织化学方法检测51例胆囊癌及15例慢性胆囊炎中MK蛋白表达情况和微血管密度(MVD)及Ki-67抗原的表达情况,并对其临床病理指标进行比较分析。结果免疫组织化学结果表明,慢性胆囊炎组织中MK蛋白无表达或微表达,51例胆囊癌组织中有35例呈阳性表达(68.6%),其表达率与慢性胆囊炎(6.7%)差异有统计学意义(P<0.01)。胆囊癌组织中平均MVD为49±15,而炎性组织中MVD为17±6,两者差异有统计学意义(P<0.01)。Ⅳ、Ⅴ期胆囊癌中MK阳性表达率明显高于Ⅰ、Ⅱ和Ⅲ期病例。Ki-67抗原在慢性胆囊炎中表达率为20%,在胆囊癌中呈高表达(78.4%),二者差异有统计学意义(P<0.01)。结论MK蛋白在胆囊癌中具有较高表达水平,其表达与胆囊癌Nevin分期,微血管密度及增殖指数具有相关性并可作为判断胆囊癌转移和细胞恶性程度的指标。

p53 ki-67和PCNA在膀胱移行细胞癌组织中的表达及意义

目的研究膀胱移行细胞癌组织中p53、ki-67和PCNA表达的关系和临床意义。方法应用免疫组织化学SP法染色检测68例膀胱移行细胞癌及36例膀胱良性病变组织中p53、ki-67和PCNA的表达情况。结果膀胱移行细胞癌组织p53、ki-67和PCNA的阳性表达率依次为51.4%(35/68)、61.7%(42/68)和80.9%(55/68),较膀胱良性病变增加,差异有统计学意义(P<0.05),而且随着组织学分级及临床分期的增加而增加;p53、ki-67及PCNA的表达具有明显的相关性。结论p53、ki-67和PCNA的表达与膀胱移行细胞癌的发生、发展有一定关系,可以作为膀胱肿瘤生物学行为和判断预后的参考指标,三者结合对肿瘤的恶性程度判断更为准确。

Localization and function of a eukaryotic-initiation-factor-2-associated 67-kDa glycoprotein

AIM: To study the localization and function of a eukaryotic initiation factor 2 (eIF2α)-associated 67-kDa glycoprotein (p67).METHODS: Immunofluorescence staining,35S-Met/Cys metabolic labeling,Western blotting analysis,sucrose gradient centrifugation and high speed centrifugation were used to determine the localization of proteins in transiently transfected COS-1 cells.Transient co-transfection followed by co-immunoprecipitation was used to study the interaction between p67 and double-stranded RNA (dsRNA)-dependent protein kinase (PKR).Wheat germ agglutinin agarose beads were used to absorb glycosylated proteins.In vivo 32P-labeling followed by immunoprecipitation and Western blotting were used to measure PKR autophosphorylation,eIF2α phosphorylation,and p67 expression in normal and breast cancer cells.RESULTS: The image from immunofluorescence staining showed that p67 was overexpressed in the cytosol but not in the nucleus.In a sucrose gradient,approxi-mately 30% of the overexpressed p67 was bound with ribosomes.p67 interacted with the kinase domain,butnot the dsRNA-binding domains of PKR.Only the glycosylated p67 was associated with the ribosome,and p67 did not compete with PKR for ribosome binding.In breast cancer cells,there was increased autophosphorylation of PKR but no phosphorylation of eIF2α,compared with normal breast cells.α The ratio of glycosylated/deglycosylated p67 was altered in breast cancer cells.CONCLUSION: Glycosylation of p67 is required for its ribosomal association and can potentially inhibit PKR via interaction with the kinase domain of PKR.

胃癌组织中CerbB-2 EGFR bcl-2 P53 Ki-67的表达及相关性

目的:分析胃癌组织中C-erb B-2、EGFR、bcl-2、P53、Ki-67的表达及相关性为胃癌的预后和靶向治疗提供理论依据。方法:回顾性分析193例胃癌患者C-erb B-2、EGFR、bcl-2、P53、Ki-67免疫组织化学的染色结果及各临床病理特征。结果:C-erb B-2、EGFR与浸润深度、淋巴结转移有关(P<0.05),但与肿瘤分化程度无关(P>0.05);P53与淋巴结转移及分化程度均有关(P<0.05),但与浸润深度无关(P>0.05);Ki-67与浸润深度、淋巴结转移、肿瘤分化程度有关(P<0.05)。同时,研究结果显示,各指标均与胃癌患者的年龄及性别无关(P>0.05)。C-erb B-2与EGFR,Ki-67与bcl-2,EGFR与Ki-67均呈正相关;bcl-2与P53呈负相关;其余指标之间无相关性。结论:胃癌组织中C-erb B-2、EGFR、bcl-2、P53、Ki-67与临床病理特征有关,联合检测对胃癌的预后和靶向治疗有重要意义。

The prognostic significance of ALI,PLR,and Ki-67 expression in stageⅢ–Ⅳinoperable non-small cell lung cancer

Objective The aim of the study was to investigate and compare the prognostic value of advanced inflammatory index,platelet/lymphocyte ratio(PLR),and Ki-67 expression in stageⅢ–Ⅳinoperable non-small cell lung cancer(NSCLC)before treatment.Methods The clinical data of 98 inoperable patients with stageⅢ–ⅣNSCLC in our hospital(Fifth Department of Oncology,Hebei General Hospital,Shijiazhuang,China)before treatment were retrospectively analyzed,and advanced lung cancer inflammation index(ALI)was calculated using body mass index(BMI)×serum albumin(ALB)÷neutrophil/lymphocyte ratio(NLR).he optimal cutoff values of ALI and PLR for predicting prognosis is determined.Chi-square test was used to analyze the relationship between patients and clinical characteristics.Kaplan-Meier method was used to calculate the total survival of patients,and log-rank test was used for comparison.Independent prognostic factors were assessed by univariate and multivariate analyses.Spearman correlation was used to analyze the relationship among ALI,PLR,and Ki-67.Results In our study of the 98 cases,the survival time of the patients with ALI<18 was significantly lower than that of patients with ALI>18(P<0.001),with a median survival time of 10 months and 25 months,respectively.The survival time of patients with a PLR<185 was significantly higher than that of patients with a PLR>185(median survival time was 27 months vs.10 months,P<0.001).The higher the Ki-67 expression,the shorter the survival time(P<0.005).The combined ALI and PLR detection results indicated that the survival time of patients with high ALI and low PLR was significantly longer than that of patients with low ALI and high PLR(P<0.001).Univariate analysis showed that smoking history,degree of differentiation,KPS score,Ki-67 expression,ALI value,and PLR affected the prognosis of patients.Multivariate analysis showed that KPS score,ALI value,and Ki-67 expression were independent prognostic factors.Conclusion ALI,PLR,and Ki-67 expression are important predictors of stage III-IV inoperable NSCLC.In terms of the prognostic value,ALI seems to have the best ability to predict patient survival.In addition,the combined detection of ALI and PLR levels before treatment seems to be more helpful in improving our prediction of patient prognosis.Moreover,it is expected to play a role in future clinical applications.

垂体腺瘤中Fascin Ki-67的表达与肿瘤侵袭性的相关研究

目的研究垂体腺瘤中Fascin、Ki-67蛋白的表达水平,并探讨它们和肿瘤侵袭性的相关性。方法选取石河子大学医学院第一附属医院既往30例侵袭性和30例非侵袭性垂体腺瘤手术蜡块标本,应用免疫组化法检测标本中Fascin、Ki-67蛋白的表达情况,统计学方法分析两种蛋白表达水平和Konsp分级的相关性。结果免疫组化结果证实,Fascin、Ki-67在侵袭性垂体腺瘤中呈阳性或强阳性表达,在非侵袭性垂体腺瘤组织中呈阴性或弱阳性表达,其表达均与Knosp分级呈正相关。结论Fascin、Ki-67蛋白可能是预测垂体腺瘤侵袭性的潜在生物学标志物。