EXPRESSION OF p16,CYCLIN D1 AND RB PROTEIN IN GASTRIC CARCINOMA AND PREMALIGNANT LESIONS

Objective: To investigate the expression of p16, cyclin D1 and Rb protein in gastric carcinoma and premalignant lesions including dysplastic gastric mucosa and intestinal metaplasia gastric mucosa. Methods: Using SP immunohistochemical methods, the expression of pl6, cyclin D1 and Rb proteins was detected in 10 specimens of normal gastric mucosa, 15 specimens of dysplastic gastric mucosa, 15 specimens of intestinal metaplasia gastric mucosa, 30 specimens of gastric carcinoma. The clinical characteristics of the 30 patients with gastric carcinoma were analysed to explore the relationship between the parameter detected and biological action of gastric cancer. Results: Expression of p16 protein was detected in 90% of normal gastric mucosa, 86.67% of dysplastic gastric mucosa, 86.67% of intestinal metaplasia gastric mucosa, 36.67% of gastric carcinoma. The positive rate of p16 protein expression in gastric carcinoma is significantly lower than that in normal gastric mucosa and gastric premalignant lesions mucosa (P<0.01). Expression of cyclin D1 protein was detected in 10% of normal gastric mucosa, 20% of dysplastic gastric mucosa, 20% of intestinal metaplasia gastric mucosa, 53.33% of gastric carcinoma. The positive rate of cyclin D1, protein expression in gastric carcinoma is significantly higher than that in normal gastric mucosa and gastric premalignant lesions mucosa (P<0.05). Expression of Rb protein was detected in 90% of normal gastric mucosa, 80% of dysplastic gastric mucosa, 80% of intestinal metaplasia gastric mucosa, 50% of gastric carcinoma. The positive rate of Rb protein expression in gastric carcinoma is significantly lower than that in normal gastric mucosa (P<0.05). The expression of p16, cyclin D1 gene were associated with the degree of differentiation of gastric carcinoma, lymphnodes metastasis and distant metastasis. Conclusion: p16, Cyclin D1 and Rb gene play important role in gastric carcinoma genesis. The expression of p16, cyclin D1 and Rb gene have some value to the diagnosis at earlier stage of gastric cancer. Detection of expression of p16, cyclin D1 gene would be helpful to judge the prognosis of gastric cancer.

EXPRESSION AND SIGNIFICANCE OF ONCOPROTEIN p16 AND FOS IN OSTEOSARCOMA

Objective: To investigate p16, C-fos protein expression and their relationships in osteosarcoma. Methods: Immunohistochemical technique (SABC) was used to detect p16 and C-fos protein expression in 41 cases of osteosarcoma. Results: The positive rates of p16 and C-fos protein expression were 51.2% and 82.9% respectively. Their expression was not correlated to pathological subtype, but correlated to clinic grade, and the latter was associated with tumor metastasis. There was a negative correlation between p16 and C-fos protein expression. Conclusion: The alteration of p16 and C-fos protein expression may be related to the tumorigenesis and development of osteosarcoma, and C-fos proteins may take part in osteosarcoma metastasis. These data will offer useful helpness to determine the prognosis of osteosarcoma.

Interaction among Rb/p16, Rb/E2F1 and HDAC1 Proteins in Gallbladder Carcinoma

The mechanism and interaction among Rb/p16, Rb/E2F1 and HDAC1 proteins in gallbladder carcinoma were investigated. By using the immunoprecipitation method, the interactions among Rb, p16, E2F1, HDAC1 proteins in gallbladder carcinoma cell line （Mz-ChA-1） were studied. It was found that there were Rb and E2F1 proteins in the precipitates with anti-HDAC1, and there were HDAC1 and E2F1 proteins in the precipitate with anti-Rb. It was concluded that there are specific interactions among Rb, HDAC1 and E2F1 proteins in gallbladder carcinoma, indicating the existence of the direct Rb/E2F1/HDAC1 signal transduction pathway. There is no direct relationship between p16 proteins with Rb, HDAC1, and E2F1 proteins.

Expressions of p16 and FHIT Proteins During Esophageal Carcinomatous Development in High Incidence Area of Esophageal Carcinoma

Objective： To detect the changes of p16 and FHIT and investigate their relationship in esophageal squamous cell carcinoma development by measuring their expression levels in normal squamous epithelium tissue, mild, moderate, severe dysplasia lesions, carcinoma in situ and invasive squamous cell carcinomas. Methods： Expressions of p16 protein and FHIT protein were detected and analyzed in 17 cases of normal squamous epithelium, 16 cases of mild dysplasia, 16 cases of moderate dysplasia, 17 cases of severe dysplasia, 10 cases of carcinoma in situ, and 18 cases of esophageal squamous cell carcinoma by immunohistochemical method. Results： With increasing histopathologic grades, the expressions of pl6 and FHIT became gradually lower. There was no remarkable difference of p16 and FHIT expressions between the normal and mild dysplasia group （P〉0.05）, but the differences between the normal and other groups were all significant （P〈0.05）. There was no remarkable difference among the squamous cell carcinoma group, the moderate and severe dysplasia groups, and the carcinoma in situ group （P〉0.05）, but significant differences existed in the expressions of p16 and FHIT proteins between the squamous cell carcinoma and the normal groups, and between the squamous cell carcinoma and the mild dysplasia groups （P〈0.05）. There was an association of descending trend between p16 and FHIT protein expressions. Conclusion： Reduced expressions of pl6 and/or FHIT proteins possible play an important role in the early occurrence of esophageal cancer. There was a positive correlation between the expressions of p16 and FHIT proteins.

Diagnostic value of HPV and P16 protein in patients with HSIL and prognosis

Objective: To investigate the diagnostic value and prognostic value of HPV and P16 protein in patients with HSIL and to provide a reference for the clinical diagnosis and assessment of the prognosis of patients with HSIL. Methods: The surgical treatment of HSIL patients from January 2013 to January 2015 in our hospital were selected. All patients were routinely tested for HPV and P16 protein, All patients were followed up for 1 year. Patients were divided into progressive group and quiescent group according to whether the disease progressed one year after surgery. Preoperative HPV and P16 protein levels were compared between the two groups. Using receiver operating curve (ROC curve) Analysis of HPV diagnostic value of HSIL. The levels of HPV and P16 protein in the two groups were analyzed and compared. Results: The quantitative level of high-risk HPV-DNA after LEEP was significantly lower than that before operation. The level of P16 protein in preoperative patients was higher than that before operation, and the difference was statistically significant. There were 21 patients in the postoperative progression group, and the average HPV-DNA content in the patients in the progression group was higher than that in the control group within one year after operation. The difference was statistically significant. The P16 protein level in patients in advanced group was significantly higher than that in resting group. Preoperative HPV-DNA levels and P16 protein levels in patients with progressive disease were significantly higher than those in still group. ROC curve analysis showed that the cut-off value of 2.441, HPV-DNA prediction of HSIL patients one year after the recurrence of the sensitivity was 95.12%, the specificity was 76.16%, under the curve area of 0.878;7.4 cut-off value, P16 The predictive value of HSIL patients recurrence after 1 year was 71.95%, specificity was 66.67%, and the area under the curve was 0.753. The recurrence group HPV-DNA content and P16 protein level showed a significant positive correlation, with statistical significance. Conclusions: LEEP can reduce the postoperative levels of HPV and P16 protein in patients with HSIL. The HPV and P16 protein levels are of high value for the early diagnosis of HSIL and the prediction of postoperative disease progression.

Association of cyclin D1, p16 and retinoblastoma protein expressions with prognosis and metastasis of gallbladder carcinoma

AIM: To investigate the role of cydin D1, p16 and retinoblastoma in cancerous process of gallbladder carcinomas and to assess the relation between cyclin D1, p16, Rb and the biological characteristics of gallbladder carcinoma.METHODS: Forty-one gallbladder carcinoma, 7 gallbladder adenoma and 14 chronic cholecystitis specimens were immunohistochemically and histopathologically investigated for the relation of cyclin D1, p16 and Rb with Nevin staging and pathologic grading.RESULTS: The expression rates of abnormal cyclin D1 in gallbladder carcinoma (68.3%)and gallbladder adenoma(57.1%) were significantly higher than those in chronic cholecystitis (7.1%) (P＜0.05). No significant difference was found both among the pathological grades G1, G2 and G3and among Nevin stagings S1-S2, S3 and S4-S5 of gallbladder carcinoma. The positive rates of p16 (48.8%) and Rb(58.5%) in gallbladder carcinoma were significantly lower compared to those in adenoma (100.0%) and cholecystitis(100.0%) (P＜0.05). The positive rates of p16 and Rb in Nevin stagings S1-S2 (80.0% and 90.0%) and S3 (46.2%and 61.5%) gallbladder carcinomas were significantly higher than those in S4-S5 (33.3% and 38.8%) (P＜0.05),and those in pathologic grades G1 (54.5% and 81.8%) and G2 (50.0% and 62.5%) gallbladder carcinoma were significantly higher than those in G3 (28.6% and 35.7%)(P＜0.05). The protein expression of p16 and Rb had a negative-correlation in gallbladder carcinoma (r = -0.2993,P＜0.05), and this negative-correlation was correlated with Nevin staging (P＜0.05). Moreover, the protein expression of p16 and cyclin D1 had a negative-correlation in gallbladder carcinoma (r = -0.9417, P＜0.05).CONCLUSION: Cyclin D1 may play a role in the early stage of gallbladder carcinoma. Mutation of p16 and Rb genes might be correlated with progression of gallbladder carcinoma.Analysis of p16 and Rb can estimate the prognosis of gallbladder carcinoma. Expression of p16 and Rb may be correlated with Nevin staging and pathologic grading in gallbladder carcinoma.

Expression of p16 gene and Rb protein in gastric carcinoma and their clinicopathological significance

AIM: To analyze the correlation between the protein expression of p16 and Rb genes in gastric carcinoma (GC),to investigate the role of p16 gene in invasion and lymph node metastasis of GC, and to examine the deletion and mutation in exon 2 of p16 gene in GC.METHODS: The protein expression of p16 and Rb genes was examined by streptavidin-peroxidase conjugated method (S-P) in normal gastric mucosa, dysplastic gastric mucosa and GC. The deletion and mutation of p16 gene were examined by polymerase chain reaction (PCR) and polymerase chain reaction single strand conformation polymorphism (PCR-SSCP) respectively in normal gastric mucosa and GC.RESULTS: The positive rates of P16 and Rb protein expression respectively were 96% (77/80) and 99%(79/80) in normal gastric mucosa, 92% (45/50) and 80%(40/50) in dysplastic gastric mucosa, 48% (58/122) and 60% (73/122) in GC. The positive rates of P16 and Rb protein expression in GC were significantly lower than that in normal gastric mucosa and dysplastic gastric mucosa (P＜0.05). The positive rate of P16 protein expression in mucoid carcinoma (10%, 1/10) was significantly lower than that in poorly differentiated carcinoma (51%, 21/41),undifferentiated carcinoma (58%, 15/26) and signet ring cell carcinoma (62%, 10/16) (P＜0.05). The positive rates of P16 protein in 30 cases of paired primary and lymph node metastatic GC were 47% (14/30) and 17% (5/30)respectively, being significantly lower in the later than in the former (P＜0.05). There was no mutation in exon 2 of p16 gene in the 25 freshly resected primary GCs. But five cases in the 25 freshly resected primary GCs displayed deletion in exon 2 of p16 gene. The positive rate of both P16 and Rb proteins was 16% (14/90), and the negative rate of both P16 and Rb proteins was 8% (7/90) in 90GCs. The rate of positive P16 protein with negative Rb protein was 33% (30/90). The rate of negative P16 protein with positive Rb protein was 43% (39/90). There was reverse correlation between P16 and Rb expression in 90GCs (P＜0.05).CONCLUSION; The loss protein expression of p16 and Rb genes is related to GC. The loss expression of P16protein is related to the histopathologic subtypes and lymph node metastasis of GC. Expression of P16 and Rb proteins in GC is reversely correlated. The deletion but not mutation in exon 2 of p16 gene may be involved in GC.

Clinicopathological significance of LRP16 protein in 336 gastric carcinoma patients

AIM:To investigate the expression of leukemia related protein 16 (LRP16), and the possible relationship between LRP16 expression and clinicopathological indices in 336 gastric carcinoma patients.METHODS: Immunohistochemistry was used to detect LRP16 expression in 336 cases of paraffin-embedded gastric carcinoma tissues and 60 cases of distal normal mucosa. The relationships between LRP16 expression and patients' age, tumor size, histological grade, clinical stage, metastatic status and prognosis were analysed.RESULTS: The expression of LRP16 was 58.6% (197/336) in gastric carcinoma and 31.7% (19/60) in distal normal gastric mucosa. The expression of LRP16 in carcinoma was significantly higher than that in normal mucosa tissues (χ2=14.929, P=0.001). LRP16 protein expression was found in 44.1% (63/143) carcinomas at stage and,and 69.4%(134/193) carcinomas at stage and (χ2=21.804,P=0.001),and in 56.9%(182/320)of cancers without metastasis but 93.8%(15/16)of those with metastasis(χ2=8.543,P=0.003).The expression of LRP16 was correlated with tumor size,infiltrative depth,clinical stage,lymphatic invasion and distant metastasis(all P<0.05). Follow-up data showed that there was a significant difference in median survival time between cancer patients with expression of LRP16 (27.0 mo) and those without (48.0 mo, Log rank=31.644, P=0.001).CONCLUSION: The expression of LRP16 may be associated with invasion, metastasis and prognosis of gastric cancer.

Change of p16^(INK4a) and PNCA Protein Expression in Myocardium after Injection of hIGF-1 Gene Modified Skeletal Myoblasts into Post-infarction Rats

This study examined the change of p16^INK4a and PNCA protein expression in myocardium after injection of hIGF-1 gene modified skeletal myoblasts into post-infarction rats. HIGF-1 gene modified skeletal myoblasts （hIGF-1-myoblasts） were injected into hind limb muscles of 18 post-infraction rats （experimental group）. Primary-myoblasts were injected into 18 post-infraction rats （control group） and 12 non-infarction rats （sham group）. Expression of p16INK4a and PCNA pro- tein in myocardiums were separately detected immunocytochemically 1, 2 and 4 weeks after the inuection. The level of hIGF-1 and rIGF-1 protein in serum and myocardium were detected by en- zyme-linked immunosorbent assay （ELISA）. Compared with the sham group, the percentage of p^16INK4a and PCNA positive cells reached a peak after 1 week in the control group and the experimental group （P〈0.01）. Moreover, the percentage of p16^INK4a-positive cells in the experimental group was lower than in control group whereas the percentage of PCNA-positive cells was lower in the control group than in the experimental group （P〈0.01）. The percentage of p16^INK4a-positive cells in the experimental group and the percentage of PCNA-positive cells in the control group were close to that in the sham group from the 2nd week （P〉0.05）. ELISA analysis disclosed that the myocardium level of rIGF-1 protein increased gradually in the controls and especially in the experimental group （P〈0.01）. The serum level of rIGF-1 decreased significantly in post-infraction rats, but these conditions were improved in the experimental group （P〈0.01）. The hIGF-1 protein in serum and myocar- dium were detected from the 1st week to the 4th week in the experimental group. Statistical analysis revealed significant associations of myocardium level of hIGF-1 protein with expression of p^16INK4a and PCNA protein （r=–0.323, P〈0.05; r=0.647, P〈0.01）. It is concluded that genetically hIGF-1-myoblast provides a means for constant synthesis and release of hIGF-1. It could not only improve the expression of rIGF-1 and PCNA protein in myocardium, but also suppress the expression of p16^INK4a protein for 30 days in post-infraction rats. Myoblasts-mediated IGF-1 gene therapy may provide a new alternative for the clinical treatment of heart failure.

The Change and Implication of p16 Protein in CA and Its Cancerization

Objective : To investigate the role and clinical significance of p16 protein in Condyloma Acuminatum (CA) and its cancerization. Methods: The expression of p16 protein was tested in 33 CA samples and 7 cancerized CA samples by immunohistochemical assays. Results: There was abnormal expression of p16 protein in CA and cancerized CA, mainly major protein expression. The p16 protein expresseed in different locations in different cases was as follows: In basal layer cells in normal cuits; in spinous layer, granular layer and stratum corneum layer cells in CA;in keratin pearl peripheral and spinous layer cells in cancerized CA. Conclusion.. There was major expression of p16 protein in CA and cancerized CA, and these protein of the two groups might not naturally be the same. Our study indicated that in clinical practice, when major p16 protein expression in CA occurs, it's risk of cancerization shoud be suspected.

温石棉对内皮细胞Wnt5a、p16和p21表达的影响

目的探讨温石棉对人脐静脉内皮细胞(HUVECs)的影响。方法实验组以50、100、200 mg/L温石棉纤维液刺激HUVECs 24、48、72 h,对照组仅加RPMI 1640培养基培养细胞,观察细胞形态变化,β-半乳糖苷酶法分析细胞衰老情况,四甲基偶氮唑蓝法检测细胞存活率。采用实时荧光定量PCR法检测细胞中Wnt5a、p16和p21 mRNA的表达情况。结果实验组HUVECs多呈梭形,部分呈圆形或不规则形,出现裸核及空泡现象,可见死亡细胞。随温石棉质量浓度及暴露时间的增加,细胞活性逐渐降低,衰老细胞逐渐增多。100 mg/L温石棉处理HUVECs 24 h时,细胞生长较活跃。与对照组相比,实验组Wnt5a、p16和p21 mRNA表达水平均增高(P<0.05)。结论温石棉可促进HUVECs衰老,Wnt5a、p16和p21参与此过程。

HPV感染病例与P16^(INK4a)表达的关联研究

目的:研究P16^(INK4a)在宫颈炎症与宫颈鳞状上皮内病变以及癌组织中的免疫表达及其与临床病理特征和HPV-DNA分型的相关性。方法:收集商丘市第一人民医院2020年4月至2023年4月404例宫颈病变组织病理活检,包括炎症组织、宫颈鳞状上皮内病变以及鳞状细胞癌(SCC);统计所有病例的P16^(INK4a)免疫组化染色与HPV-DNA分型结果,并分析各病变组织中的P16^(INK4a)与HPV分型关系。结果:P16^(INK4a)的表达在各种宫颈病变组织中存在显著差异(P<0.05),且P16^(INK4a)的阳性率与表达强度随宫颈病变程度的加深而增加;宫颈组织中P16^(INK4a)的表达在低危型与高危型HPV间有显著差异(P<0.05),P16^(INK4a)的表达强度与高危型HPV有相关性(P<0.001)。结论:P16^(INK4a)免疫染色结合HPV-DNA分型能有助于鉴别高级别CIN和宫颈癌,以及区分这些病变与低级别CIN和正常宫颈组织。

p16/Ki-67双染检测、HPVE6/E7mRNA检测在宫颈病变诊断中的价值研究

目的:探讨p16/Ki-67双染检测、HPVE6/E7mRNA检测在宫颈病变诊断中的价值。方法:选择2021年4月至2023年2月我院收治的605例高危型HPV阳性患者为研究对象,所有患者入院后均进行阴道镜检查与病理活检,取患者细胞学样本分别进行p16/Ki-67双染检测、HPVE6/E7mRNA检测。比较两种方法单独检查与联合检测对宫颈病变阳性检出率的差异;比较三种检查方法对宫颈病变诊断效能的差异。结果:联合检测检出CIN1~3阳性率均高于p16/Ki-67双标记、HPVE6/E7mRNA检测(P<0.05);三种检测方式的浸润癌阳性检出率比较无明显差异(P>0.05)。Kappa检验分析结果显示,p16/Ki-67双标记与HPVE6/E7mRNA检查结果与病理诊断结果具有较好的一致性(Kappa值=0.719,P<0.05);联合检测结果与病理诊断结果具有高度的一致性(Kappa值=0.894,P<0.05)。联合检测对宫颈病变的诊断灵敏度、特异性与准确率高于p16/Ki-67双标记与HPVE6/E7mRNA检查(P<0.05)。结论:p16Ki-67双染与HPVE6/E7mRNA联合检测可准确检出宫颈病变程度,对宫颈病变疾病类型的鉴别可提供充足可靠的数据支持,联合检查的诊断效能较好,对患者后续相关治疗开展有积极指导意义。

P53、P16及Ki-67与早期食管癌患者ESD术后复发的关系分析

目的 分析研究抑制蛋白基因P53(P53)、抑制蛋白基因P16(P16)及细胞增殖核抗原Ki-67(Ki-67)与早期食管癌患者内镜黏膜下剥离术(ESD)术后复发的关系。方法 选取2019年1月至2023年1月三门峡市中心医院收治80例的早期食管癌患者作为研究对象,均进行ESD治疗。比较所有患者癌组织与癌旁组织(距肿瘤边缘>3 cm,镜下未见肿瘤组织或不典型增生组织)P53、P16、Ki-67蛋白表达。ESD术后对患者随访1年,根据有无术后复发食管癌,将患者分为复发组(n=20)与无复发组(n=60)。比较两组癌组织P53、P16、Ki-67蛋白表达。采用多因素Logistic回归分析早期食管癌患者ESD术后复发的影响因素,并绘制ROC曲线分析P53、P16、Ki-67蛋白表达与早期食管癌患者ESD术后复发。结果 与癌旁组织相比,癌组织P53、Ki-67蛋白表达升高,P16蛋白表达降低(t=9.276、13.987、10.595,均P<0.05);复发组的癌组织P53、Ki-67蛋白表达均高于无复发组,P16蛋白表达低于无复发组(t=5.086、4.648、5.139,均P<0.05);多因素Logistic回归分析显示,肿瘤低分化(OR=1.870)、肿瘤浸润侵犯黏膜下层(OR=1.808)、有淋巴结转移(OR=2.089)、P53蛋白高表达(OR=2.046)、P16蛋白低表达(OR=1.988)及Ki-67蛋白高表达(OR=1.761)均是早期食管癌患者ESD术后复发的独立危险因素(P<0.05);ROC曲线分析显示,P53、P16、Ki-67蛋白表达及联合检测的曲线下面积(AUC)分别为0.803、0.828、0.834、0.942,联合检测优于单一检测(P<0.05)。结论 P53、P16及Ki-67蛋白与早期食管癌患者ESD术后复发情况相关,可以作为辅助预测的相关诊断指标。

DNA倍体定量分析结合P16蛋白在宫颈癌筛查中的意义

目的:探讨DNA定量分析结合P16蛋白免疫组化染色在宫颈筛查中的意义。方法:选取2020年3月~2021年3月淮安市肿瘤医院门诊及住院检查病例4334例为研究对象,对宫颈细胞学DNA定量分析提示异常者,在知情同意情况下,进行阴道镜下宫颈活检、组织学病理检查及P16蛋白染色检查,比较分析DNA倍体定量分析提示异常者与其组织学病理之间的关系,以及P16蛋白表达与病理组织学之间的关系,以评价衡量DNA定量分析结合P16染色在宫颈癌筛查中的意义。结果:4334例中369例查出倍体异常,369例倍体异常中202例建议阴道镜活检,发现150例为CIN1级以上,同时对活检202例进行P16蛋白染色发现随着病变级别增高,P16表达百分比也随之上升,通过χ2检验发现P16蛋白在宫颈上皮内瘤变中表达有明显统计学意义(P<0.05)。结论:DNA倍体定量分析在宫颈癌筛查中有一定意义,同时结合P16蛋白免疫组化染色及宫颈活检,对发现早期宫颈病变有意义。

HPV阳性女性中宫颈液基细胞学、DNA倍体分析及P16/Ki-67双染检测对宫颈癌前病变的分流效果研究

目的探讨在HPV阳性女性中,液基细胞学、DNA倍体分析及P16/Ki-67双染检测对宫颈癌前病变的分流作用。方法回顾性分析2021年5月至2022年12月在我院妇科行阴道镜及宫颈活检的妇女590例。患者高危人乳头瘤病毒(human papilloma virus,HPV)检测阳性,且行液基细胞学(liquid-based cytology,LBC)、DNA倍体分析、P16/Ki-67双染3种检查,对上述3种方法的灵敏度、特异性、受试者工作特征(receiver operating characteristic,ROC)曲线进行统计分析。结果液基细胞学、DNA倍体分析和P16/Ki-67双染3种筛查方法对宫颈癌前病变的灵敏度分别为84.2%、77.5%、76.4%,特异性分别为40.7%、49.2%、70.1%,曲线下面积(area under the curve,AUC)分别是0.625、0.634、0.733,其中,P16/Ki-67双染检测显著优于液基细胞学检查及DNA倍体分析(P<0.0001)。结论本研究认为,在HPV阳性女性中,P16/Ki-67双染检测的分流效果最佳。

PAX1甲基化与p16/Ki-67联合检测提高宫颈癌非典型鳞状细胞的诊断效能

目的 比较配对盒家族基因1(paired boxed gene 1,PAXl)甲基化与p16/Ki-67双染单独及联合检测在薄层液基细胞学检查(thinprep cytologic test, TCT)为意义不明的非典型鳞状细胞(atypical squamous cells of undetermined significance, ASC-US)及低级别鳞状上皮内病变(low-grade squamous intraepithelial lesion, LSIL)人群中的诊断效能。方法 选择郑州大学第一附属医院2021-2022年247例TCT结果为ASC-US和LSIL患者,以阴道镜下病理结果为金标准,敏感度、特异性、准确度与ROC曲线下面积(area under the subject curve, AUC)为评价指标,比较PAX1甲基化和p16/Ki-67双染单独及联合检测的检验效能。结果 PAX1甲基化与p16/Ki-67双染的阳性率随着阴道镜下病理结果的严重程度而增加,PAX1甲基化和p16/Ki-67联合检测在TCT为ASC-US人群中的敏感度为91.25%、特异度为97.72%、准确度为95.51%,优于甲基化、p16/Ki-67双染单独检测,差异有统计学意义(P<0.01)。结论 PAX1甲基化和p16/Ki-67双染联合检测可以提高TCT结果为ASC-US患者的诊断效能。

基于p16/ki67细胞学双染检测宫颈癌前病变的研究

目的探讨p16/ki67细胞学双染检测在宫颈病变筛查中的应用价值。方法收集妇科宫颈病变门诊患者56例为研究对象,所有患者均行宫颈液基细胞学检查(TCT)、人乳头瘤状病毒(HPV)检测、阴道镜活检,并进行p16/ki67双染,分析比较双染与TCT、HPV检查诊断宫颈病变的效能,并研究p16/ki67双染诊断宫颈病变的价值。结果在细胞学为非侵入式负荷监测(NILM)、不典型鳞状细胞(ASC-US)、低级别鳞状上皮内病变(LSIL)以上病变的分级患者中p16/ki67双染阳性的比例分别是15.38%、66.67%、92%,不同宫颈组织学诊断结果中慢性宫颈炎、宫颈上皮内瘤变Ⅰ级(CINⅠ)、宫颈上皮内瘤变Ⅱ级(CINⅡ)和Ⅲ级(CINⅢ)的患者中p16/ki67双染阳性率分别为6.66%、75%、78.57%、100%,总体差异有统计学意义(P<0.05)。结论p16/ki67免疫细胞化学染色阳性率与宫颈癌前期病变的严重程度呈现正相关。P16/ki67双染较TCT和HPV提高了宫颈病变筛查的灵敏性和特异性,并提高了高级别鳞状上皮内病变(HSIL)的检出率,减少阴道镜的转诊率,值得应用于临床。

宫颈液基细胞P16、Ki-67双染检测在1250例宫颈异常患者诊断中的诊断价值

目的:探讨与分析宫颈液基细胞P16、Ki-67双染检测在1250例宫颈异常患者诊断中的诊断价值。方法:选取2020年1月至2023年8月在河南省周口市中心医院进行宫颈疾病筛查的宫颈异常人群1250例作为研究对象,所有患者都给予宫颈液基细胞P16、Ki-67单染与双染检测,同时给予病理检查,根据病理学检测结果分为宫颈癌组(150例)和非宫颈癌组(1100例)。以病理检查作为诊断的金标准,判断以上检测方法对宫颈癌的诊断价值。结果:在1250例患者中,病理检查为宫颈上皮内瘤变(CIN)1级600例、CIN 2级320例、CIN 3级180例及宫颈癌150例,其中宫颈癌占比12.00%。宫颈癌组宫颈液基细胞P16、Ki-67单染阳性与P16、Ki-67双染阳性分别为98.00%、98.67%、97.33%,非宫颈癌组分别为40.55%、49.27%、21.00%,两组对比,差异有统计学意义(P<0.05)。在1250例患者中,宫颈液基细胞P16、Ki-67单染阳性与P16、Ki-67双染阳性对宫颈癌的诊断敏感性分别为98.00%(147/150)、98.67%(148/150)、97.33%(146/150),诊断特异性分别为59.45%(654/1100)、50.73%(558/1100)、79.00%(869/1100)。结论:宫颈异常患者中宫颈癌的发病率比较高,其宫颈液基细胞P16、Ki-67双染多表现为阳性状况,宫颈液基细胞P16、Ki-67双染检测在宫颈癌患者诊断中的应用可在保持敏感性的基础上,提高诊断特异性。