Over 200,000 Americans have peripheral nerve injuries annually that result in a loss of function and a compromised quality of life.Of these,a significant percent involves unsuccessful repair of peripheral nerve gaps t...Over 200,000 Americans have peripheral nerve injuries annually that result in a loss of function and a compromised quality of life.Of these,a significant percent involves unsuccessful repair of peripheral nerve gaps that occur due to traumatic limb injury or collateral damage to peripheral nerves during tumor resection.展开更多
The peripheral nervous system is able to regenerate after injury, and regeneration is associated with the expression of many genes and proteins. MicroRNAs are evolutionarily conserved, small, non-coding RNA molecules ...The peripheral nervous system is able to regenerate after injury, and regeneration is associated with the expression of many genes and proteins. MicroRNAs are evolutionarily conserved, small, non-coding RNA molecules that regulate gene expression at the level of translation. In this paper, we focus on the identification and functional annotation of novel microRNAs in the proximal sciatic nerve after rat sciatic nerve transection. Using Solexa sequencing, computational analysis, and quantitative reverse transcription PCR verification, we identified 98 novel microRNAs expressed on days 0, 1, 4, 7, and 14 after nerve transection. Furthermore, we predicted the target genes of these microRNAs and analyzed the biological processes in which they were involved. The identified biological processes were consistent with the known time-frame of peripheral nerve injury and repair. Our data provide an important resource for further study of the role and regulation of microRNAs in peripheral nerve injury and regeneration.展开更多
文摘Over 200,000 Americans have peripheral nerve injuries annually that result in a loss of function and a compromised quality of life.Of these,a significant percent involves unsuccessful repair of peripheral nerve gaps that occur due to traumatic limb injury or collateral damage to peripheral nerves during tumor resection.
基金supported by the National High Technology Research and Development Program of China (Grant No. 2006AA02A128)the National Natural Science Foundation of China (Grant No. 30870811)+1 种基金the Jiangsu Provincial Natural Science Foundation (Grant No. BK2008010)a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
文摘The peripheral nervous system is able to regenerate after injury, and regeneration is associated with the expression of many genes and proteins. MicroRNAs are evolutionarily conserved, small, non-coding RNA molecules that regulate gene expression at the level of translation. In this paper, we focus on the identification and functional annotation of novel microRNAs in the proximal sciatic nerve after rat sciatic nerve transection. Using Solexa sequencing, computational analysis, and quantitative reverse transcription PCR verification, we identified 98 novel microRNAs expressed on days 0, 1, 4, 7, and 14 after nerve transection. Furthermore, we predicted the target genes of these microRNAs and analyzed the biological processes in which they were involved. The identified biological processes were consistent with the known time-frame of peripheral nerve injury and repair. Our data provide an important resource for further study of the role and regulation of microRNAs in peripheral nerve injury and regeneration.
