夏枯草(Prunella vulgaris)组织培养和快速繁殖

以夏枯草的叶片、茎节、顶芽为外植体在含不同浓度的6-BA和NAA的MS培养基中培养,比较其增值率和分化率。发现以茎节为外植体,诱导不定芽的最佳培养基为MS+3.0mg/L6-BA+0.1mg/L NAA,分化率为100%,增殖倍数为6.8。以顶芽为外植体,不定芽的最佳培养基为MS+3.0mg/L6-BA+0.05mg/LNAA或MS+3.0mg/L6-BA+0.1mg/LNAA,分化率分别为88.2%和84.6%,增殖倍数分别为5.8和6.2。最佳生根培养基为1/4MS+NAA0.5mg/L,生根率为100%。将生根的组培苗经过驯化移栽后,成活率为93%。表明,叶片难以分化;茎节的分化率效果最好;顶芽的分化能力介于二者之间。

欧洲千里光(Senecio vulgaris Linn.)对干旱环境的适应

采用石蜡切片法,研究了欧洲千里光(Senecio vulgaris Linn.)营养器官的显微结构,以探讨其对干旱环境的适应性特点。结果表明:根和茎木质部导管发达,提高了输导能力;薄壁组织丰富,提高了储水能力;叶片表皮细胞外切向壁角质层发达,有利于减少水分散失;同化组织发达,能够适应干旱环境对光合作用的负面影响。欧洲千里光长期生活在干旱环境中,产生了许多适应性结构。

襄阳夏枯草(Prunella vulgaris)群落基本特征

报道了湖北省襄阳市一野生夏枯草种群。种群面积为8～10 m2,植株数为30～40株,为小种群。频度为100%,盖度为10%,密度约4株/m2。果穗平均数为5.6个/株。过度耕作和采摘是导致夏枯草中药材资源减少的重要原因。

Orally administered extract from Prunella vulgaris attenuates spontaneous colitis in mdr1a^(-/-) mice

AIM: To investigate the ability of a Prunella vulgaris(P. vulgaris) ethanolic extract to attenuate spontaneous typhlocolitis in mdr1a-/- mice. METHODS: Vehicle(5% ethanol) or P. vulgaris ethanolic extract(2.4 mg/d) were administered daily by oral gavage to mdr1a-/- or wild type FVBWT mice from 6 wk of age up to 20 wk of age. Clinical signs of disease were noted by monitoring weight loss. Mice experiencingweight loss in excess of 15% were removed from the study. At the time mice were removed from the study, blood and colon tissue were collected for analyses that included histological evaluation of lesions, inflammatory cytokine levels, and myeloperoxidase activity. RESULTS: Administration of P. vulgaris extracts to mdr1a-/- mice delayed onset of colitis and reduced severity of mucosal inflammation when compared to vehicle-treated mdr1a-/- mice. Oral administration of the P. vulgaris extract resulted in reduced(P < 0.05) serum levels of IL-10(4.6 ± 2 vs 19.4 ± 4), CXCL9(1319.0 ± 277 vs 3901.0 ± 858), and TNFα(9.9 ± 3 vs 14.8 ± 1) as well as reduced gene expression by more than two-fold for Ccl2, Ccl20, Cxcl1, Cxcl9, IL-1 α, Mmp10, VCAM-1, ICAM, IL-2, and TNFα in the colonic mucosa of mdr1a-/- mice compared to vehicle-treated mdr1a-/-mice. Histologically, several microscopic parameters were reduced(P < 0.05) in P. vulgaris-treated mdr1a-/-mice, as was myeloperoxidase activity in the colon(2.49 ± 0.16 vs 3.36 ± 0.06, P < 0.05). The numbers of CD4+ T cells(2031.9 ± 412.1 vs 5054.5 ± 809.5) and germinal center B cells(2749.6 ± 473.7 vs 4934.0 ± 645.9) observed in the cecal tonsils of P. vulgaris-treated mdr1a-/- were significantly reduced(P < 0.05) from vehicle-treated mdr1a-/- mice. Vehicle-treated mdr1a-/- mice were found to produce serum antibodies to antigens derived from members of the intestinal microbiota, indicative of severe colitis and a loss of adaptive tolerance to the members of the microbiota. These serum antibodies were greatly reduced or absent in P. vulgaris-treated mdr1a-/- mice. CONCLUSION: The anti-inflammatory activity of P. vulgaris ethanolic extract effectively attenuated the severity of intestinal inflammation in mdr1a-/- mice.

Prunella vulgaris L. extract improves cellular immunity in MDR-TB challenged rats

Objective： To study the effect of the extract of Prunella vulgaris L. on multiple drugs resistant bacillus tuberculosis （MDR-TB）. Methods： Experimental animal model in rats was induced by MDR-TB. Normal group model group and Prunella vulgaris L. group were set up. The contents of IFN-7, IL-4, IL-10 and IL-12 were examined by ELISA. Their genome mRNAs were extracted, the target genes were amplified by PCR. RT-PCR was used to detect the mRNA levels of them. Results： The content of IFN-q, of the extract of Prunella vulgaris L. group was 1.98±0.67 pg/ml, IL-4 was 6.47±1.46 pg/ml, IL-10 was 12.13±3.43 pg/ml and IL-12 was 3.02±0.86 pg/ml. Compared with the model group, Prunella vulgaris L. group was notable difference in serum IFN-γ, IL-12 and IL-10 （P〈0.05）. The mRNA levels of IFN-γ, IL-12 increased and IL-10 decreased obviously, the differences were quite significant （P〈0.05）, but IL-4 had no obvious change. Conclusion： The extract of Prunella vulgaris L. can enhance the cellar immunological function in rats from up-regulation of the level of genetic transcription, accordingly provide the theory basis of healing of tuberculosis with it.

The effects and mechanism of action of Prunella vulgaris L extract on Jurkat human T lymphoma cell proliferation

Objective:The aim of this study was to observe the effect of the Prunella vulgaris L extract on the Jurkat human T lymphoma cell line.Methods:Jurkat cells were cultivated with different concentrations of the extract from Prunella vulgaris L.The MTT assay and flow cytometry were employed to determine the cells' proliferation inhibition ratio and the apoptosis rates,respectively.Agarose gel electrophoresis was used to observe cellular DNA fragmentation,and western blotting was used to observe changes in Bcl-2 and Bax protein expression.Results:The Prunella vulgaris L extract remarkably inhibited the proliferation of Jurkat cells.This inhibition exhibited dose dependence,with an IC50 of 20.23 ± 0.31 μg/mL.Agarose gel electrophoresis showed that the apoptosis strap became wider and brighter,and flow cytometry showed that the apoptosis rate increased in a concentration-dependent manner.Western blotting showed that Bcl-2 protein was down-regulated and Bax protein was up-regulated during apoptosis.Conclusion:The extract from Prunella vulgaris L induced apoptosis of Jurkat cells by down-regulating Bcl-2 protein and up-regulating Bax protein.These actions inhibited the growth of Jurkat cells.

A Metabolomics Study of the Volatile Oil from Prunella vulgaris L.on Pelvic Inflammatory Disease

Objective Pelvic inflammatory disease(PID)is one of the most common gynaecological diseases.Here,this thesis aims to investigate the therapeutic effects of Prunella vulgaris L.oil on the PID by using metabolomics based on gas chromatographymass spectrometry(GC-MS)to address this challenge.Methods First,measurements of pro-inflammatory cytokines and histological analysis of the uterus were conducted to validate the successful generation of a PID rat model.Furthermore,the volatile oil from Prunella vulgaris L.was administered to treat PID rats.Serum samples were collected before and after treatment and analyzed by GC-MS to generate metabolite profiles for each sample.The information generated from the qualitative and quantitative analysis of these metabolites was applied to distinguish between the PID model and normal control groups.Results Some metabolites,such as acetic acid,succinic acid,glyceric acid,(R*,S*)-3,4-dihydroxybutanoic acid,3-hydroxyphenylacetic acid,D-ribose and myo-inositol showed a higher contribution in the classification model;thus,they can be considered as potential biomarkers.Furthermore,the therapeutic effect of the volatile oil extracted from Prunella vulgaris L.could also be visualized using GC-MS-based metabolomics.Conclusions The results show that metabolomics studies are invaluable for disease diagnosis and therapeutic effect estimation.

Mechanism of Prunella vulgaris in treating Graves' disease based on network pharmacology

Objective:To use the network pharmacology method to screen the effective components and targets of Prunella vulgaris to treat Graves disease,and to explore the relationship between its components and targets and diseases.Methods:Collect and screen active components and targets of Prunella vulgaris through TCMSP;use GeneCards and DisGeNET databases to screen disease-related genes of Graves disease,construct a"drug-component-disease-target"network and screen The core target of Prunella vulgaris to treat Graves disease.Then further protein interaction network analysis(PPI),GO biological function annotation,KEGG pathway analysis and molecular docking verification.Results:The ten active components of Prunella vulgaris can control 57 Graves'disease targets such as PPARG,PIK3CG,CASP9,AKT1,TNF,ICAM1,BCL2,BAX,etc.,affecting the inflammatory response,negative regulation of apoptosis and other related organisms.Academic process and TNF signaling pathway,HIF-1 signaling pathway,PI3K-Akt signaling pathway and cancer-related pathways and other related signaling pathways,thus playing a role in the treatment of Graves disease.Conclusion:This study initially verified the target and mode of action of Prunella vulgaris for Graves disease,which can lay the foundation for further revealing its clinical mechanism of action.

Review of the Studies on the Anti-Tumoral Effect of <i>Prunella vulgaris</i>

Prunella vulgaris (PV) is a herb which grows widely around the world. It is used in traditional medicine in different continents worldwide. This article reviewed the research studies in the last three decades about the use of this herb in the treatment of cancer. Specifically, this study concentrates on the scientific in-vitro methods used, as the in-vitro methods were the most preferred methods used in the past. Cell viability/apoptosis, migration, anti-oxidative activities, and the underlying molecular mechanisms were the features which most of the research focused. The aim of this article was to summarize on what molecular mechanisms, which these previous research found responsible for the anti-tumoral effect of PV. The assays to investigate the aforementioned items were organized and displayed, including the proteomic methods which study the underlying molecular mechanisms. By categorizing and organizing these methods, the directions and emphases taken by the research efforts were revealed.

The Characterization of Steam Distillation as an Extraction Method to Extract Volatile Compounds from <i>Prunella vulgaris</i>and the Investigation of Their Anti-Tumorous Effect

Prunella vulgaris (PV) is a perennial plant which is widely grown around the world. It has been widely used as a medicinal treatment for generations. Previous studies showed extracts from this plant had a wide range of therapeutic efficacy, including anti-tumorous effect. However, the volatile organic compounds (VOCs) extracted from it were rarely explored. This paper reports on the characterization of a steam distillation process to extract VOCs in PV and also the anti-tumorous effects of the PV distillate using the tetrazolium-based Cell Counting Kit-8 (CCK-8) as the test agent, when the VOCs were used to treat oral squamous cancer cells, SSC154. It was found that most abundant VOCs came out steadily and continuously for as long as the duration of the steam extraction could extend. However, some compounds such as benzaldehyde did show depletion as the distillation process progressed, while some compounds such as caryophyllene oxide was only sparsely found at the beginning of distillation. The PV distillate was mildly effective in its cytotoxicity to cancer cells SCC154, in a dosage dependent manner.

夏枯草制剂治疗Graves病的有效性与安全性的系统评价

目的系统评价口服夏枯草制剂联合西医治疗Graves病的疗效及安全性,以期为Graves病的临床诊治提供循证医学证据。方法文章全面系统检索中文学术期刊全文数据库(CNKI)、中国生物医学文献数据库(CBM)、万方数据库(WanfangData)、维普中文期刊全文数据库(VIP)四大中文数据库和PubMed、Embase、CochraneLibrary三大英文数据库,检索时间均从建库至2022年08月。采用Cochrane系统偏倚风险评估工具对纳入研究进行质量评估,并用RevMan 5.4.1软件对结局指标进行Meta分析。结果最终纳入8项随机对照试验,共784例患者。试验组均为口服夏枯草制剂联合西医治疗,对照组均为西医治疗。Meta分析结果表明,口服夏枯草制剂联合西医治疗在甲状腺功能及自身抗体的改善、缩小甲状腺腺体等方面的临床治疗总有效率高于对照组(RR=1.13,95%CI[1.10,1.26],P<0.00001),并且能够显著改善甲状腺肿的临床症状(MD=-0.39,95%CI[-0.67,-0.11],P=0.006),降低血清FT3水平(MD=-1.76,95%CI[-3.10,-0.43],P=0.01)及血清FT4水平(MD=-3.89,95%CI[-6.79,-1.00],P=0.008),提高血清促甲状腺激素(TSH)水平(MD=0.45,95%CI[0.05,0.86],P=0.03),改善甲状腺肿大情况(MD=-0.39,95%CI[-0.67,-0.11],P=0.006),缩小甲状腺左叶前后径(MD=-0.31,95%CI[-0.57,-0.06],P=0.02)、左叶左右径(MD=-0.24,95%CI[-0.32,-0.16],P<0.00001)、右叶前后径(MD=-0.30,95%CI[-0.47,-0.13],P=0.0004)、右叶左右径(MD=-0.21,95%CI[-0.33,-0.10],P=0.0004)。结论夏枯草制剂联合西医治疗对比单用西医治疗Graves病在整体疗效、改善甲状腺肿的临床症状、甲状腺激素水平,缩小甲状腺腺体有明显的优势,且较安全,但仍需后续大样本、高质量文献的支持。

整合网络药理学和体外实验探究夏枯草-半枝莲药对治疗乳腺癌的作用及机制

目的采用网络药理学和体外细胞实验探究夏枯草-半枝莲药对抗乳腺癌的作用机制。方法运用中药系统药理学数据库与分析平台筛选出夏枯草-半枝莲药对的有效成分和靶点;利用GeneCards、OMIM数据库找到乳腺癌靶点,进而构建药物-活性成分-关键靶点网络和蛋白-蛋白相互作用(PPI)网络;运用R语言进行GO功能和KEGG通路富集分析以及生存分析;将筛选出的活性成分和核心靶点进行分子对接验证;采用CCK-8法检测细胞活力;EdU、流式实验检测细胞增殖和凋亡情况;Western blot法检测p-AKT1、AKT1、β-catenin和c-MYC的蛋白表达水平。结果通过数据分析共筛选出有效成分36个,交集靶点105个,其核心成分是槲皮素、木犀草素、山奈酚、汉黄芩素、黄芩素;通过PPI和生存分析得出其关键靶点是AKT1、ESR1、CASP3、MYC;GO分析共包含4303条富集结果,KEGG分析共包含232条通路;分子对接显示核心成分与关键靶点均具有较强的结合能力。细胞实验证明,核心活性成分槲皮素可以抑制乳腺癌细胞的增殖并促进其凋亡(P<0.05),下调p-AKT1、β-catenin和c-MYC蛋白的表达水平(P<0.05)。结论夏枯草-半枝莲药对中活性成分槲皮素可能通过AKT1/β-catenin信号通路来发挥作用,为其治疗乳腺癌的作用机制研究提供了科学参考。

基于GC-MS的血清代谢组学探究夏枯草茎叶酚酸抗慢性盆腔炎的作用机制

目的基于GC-MS的血清代谢组学探究夏枯草茎叶酚酸抗慢性盆腔炎的有效成分及其作用机制。方法本实验采用60只雌性大鼠,采用混合菌液加机械损伤对40只大鼠进行慢性盆腔炎造模处理,正常组10只大鼠不做处理,假手术组10只大鼠只做机械损伤。造模成功后,将模型大鼠分为模型组、康妇炎胶囊组、头孢克洛分散片组和夏枯草茎叶酚酸组,每组10只,连续给药21 d;取正常组、假手术组、模型组和夏枯草茎叶酚酸组、康妇炎胶囊组、头孢克洛分散片组大鼠血清进行代谢组学分析,并对筛选后的差异表达基因进行GO功能分析和KEGG通路富集分析。结果夏枯草茎叶酚酸能修复慢性盆腔炎组织病理损伤;代谢组学鉴定出47种代谢物,主要以糖类、氨基酸、有机酸、脂肪酸、多元醇等多种内源性物质为主;多因素分析表明,夏枯草可改善炎症大鼠血清代谢产物异常。主要差异代谢通路为氨基酸代谢、抗坏血酸和醛糖二酸代谢途径,主要差异代谢物为尿素、葡萄糖酸、D-葡萄糖醛酸、L-赖氨酸、甘氨酸、L-丝氨酸、L-苏氨酸、L-脯氨酸、L-苯丙氨酸等,还影响机体苯丙氨酸、酪氨酸和色氨酸生物合成,抗坏血酸和醛糖二酸代谢,苯丙氨酸代谢,花生四烯酸代谢,甘氨酸、丝氨酸和苏氨酸代谢。GO功能分析表明,对激素的反应、细胞对氮化合物的反应和磷酸化的正向调节等是主要参与的生物过程。KEGG通路富集结果显示,PI3K-Akt信号通路、甲状腺激素信号通路和内分泌抵抗等为主要富集的通路。结论夏枯草茎叶酚酸对慢性盆腔炎大鼠具有明显的治疗作用,其机制可能与作用于COBT、ADH1C、MAOB等靶点,干预PI3K-Akt信号通路、甲状腺激素信号通路和内分泌抵抗等信号通路,引起差异代谢物变化有关。

夏枯草抑制人胃癌细胞上皮间质化的机制

目的探讨夏枯草对胃癌细胞上皮间质化(epithelial mesenchymal transitions,EMT)的抑制作用及其可能的作用机制。方法选取人胃癌细胞株HGC-27,将细胞随机分为对照组及夏枯草低、中、高剂量组(20、40、80μg·mL^(−1)夏枯草提取物)。用四甲基偶氮唑盐[3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazolium bromide,MTT]法检测细胞增殖抑制率;划痕实验检测细胞迁移率;Transwell小室实验检测细胞侵袭能力;反转录·聚合酶链反应(reverse transcription-polymerase chain reaction,RT-PCR)检测miR-155、糖原合成酶激酶-3β(glycogen synthase kinase-3β,GSK-3β)和β连环蛋白(β-catenin)mRNA表达情况;蛋白质印迹法(Western blotting)检测GSK-3β、β-catenin、E-钙黏蛋白(E-cadherin)、神经性钙黏蛋白(N-cadherin)、波形蛋白(Vimentin)和锌指蛋白(Snail)表达情况。结果与对照组比较,夏枯草3组胃癌细胞增殖抑制率、GSK-3βmRNA、GSK-3β和E-cadherin蛋白表达水平升高,细胞迁移率、细胞侵袭数量、miR-155、β-catenin mRNA水平、β-catenin、N-cadherin、Vimentin和Snail蛋白的表达水平降低(P<0.05)。与夏枯草低剂量组比较,夏枯草中剂量组、夏枯草高剂量组胃癌细胞增殖抑制率、GSK-3βmRNA、GSK-3β和E-cadherin蛋白的表达水平升高,细胞迁移率、细胞侵袭数量、miR-155、β-catenin mRNA水平、β-catenin、N-cadherin、Vimentin和Snail蛋白的表达水平降低(P<0.05),夏枯草高剂量组诸项指数变化尤其明显(P<0.05)。结论夏枯草对胃癌细胞增殖、迁移和侵袭有一定的抑制作用,并能有效阻断胃癌细胞发生EMT,可能是通过降低miRNA-155表达水平、抑制Wnt/β-catenin信号通路发挥作用。

乳癖消片治疗乳腺良性结节的效果及对乳腺高频超声指标的影响

目的:探讨乳癖消片治疗乳腺良性结节的效果及对乳腺高频超声指标的影响。方法:选取2023年9~12月期间于某院诊断为乳腺良性结节的60例患者作为研究对象,采用随机数字表法分为对照组和观察组,每组30例。对照组患者给予夏枯草口服液治疗,观察组患者给予乳癖消片治疗,两组均连续服用2个月。比较两组患者临床症状改善时间、性激素(雌二醇、催乳素和孕酮)水平、乳腺高频超声指标(乳腺低回声区直径、囊肿直径、输乳管内径和乳腺腺体层厚度)、临床疗效及不良反应发生情况。结果:治疗后,观察组患者乳房肿块消退时间、乳房压痛消失时间均短于对照组(P<0.05)。两组患者雌二醇和催乳素水平均降低(P<0.05),且观察组低于对照组(P<0.05);两组患者孕酮水平均升高(P<0.05),且观察组高于对照组(P<0.05)。两组患者乳腺低回声区直径、囊肿直径、输乳管内径和乳腺腺体层厚度均降低(P<0.05),且观察组低于对照组(P<0.05)。观察组患者临床治疗总有效率(96.67%)高于对照组(73.33%,P<0.05)。两组患者不良反应总发生率比较无统计学差异(P>0.05)。结论:乳癖消片治疗乳腺良性结节患者临床疗效较好,可快速消除患者乳房肿块、疼痛等临床症状,改善性激素水平和乳腺高频超声指标,促进乳腺增生结节消散。

夏枯草口服液联合常规西药治疗格雷夫斯甲状腺功能亢进症的效果观察

目的:探讨夏枯草口服液联合常规西药治疗格雷夫斯甲状腺功能亢进症(GH)的效果。方法:选取2020年1月—2023年1月揭阳市揭西县人民医院收治的208例GH患者作为研究对象,随机分为对照组(n=106)和治疗组(n=102)。对照组实施常规西药治疗,治疗组在对照组基础上应用夏枯草口服液治疗。比较两组甲状腺功能、肝功能、不良反应发生情况。结果:治疗前后,两组血清游离甲状腺素(FT_(4))、游离三碘甲状腺原氨酸(FT_(3))、促甲状腺激素(TSH)水平比较,差异无统计学意义(P>0.05);治疗后,两组FT_(3)、FT_(4)水平低于治疗前,TSH水平高于治疗前,差异有统计学意义(P<0.05)。治疗前,两组丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)水平比较,差异无统计学意义(P>0.05);治疗后,治疗组ALT、AST水平低于治疗前和同期对照组,差异有统计学意义(P<0.05)。两组用药不良反应发生率比较,差异无统计学意义(P>0.05)。结论:夏枯草口服液联合常规西药治疗GH的效果显著,可改善患者肝功能与甲状腺功能,安全性较高。

The Effect of <i>Prunella</i>on Anti-Inflammatory Activity in RAW264.7 Mouse Macrophage Cells

The extracts of Prunella vulgaris L. (Labiatae), a popular Western and Chinese herbal medicine, was shown to have anti-inflammatory properties, which might be due to partially, their rosmarinic acid content. Inhition of prostaglandine E2 (PGE2) production in lipopolysaccharide (LPS) stimulated RAW264.7 mouse macrophage cells was assessed with an enzyme immunoassay (EIA) following 8-hour treatments with Prunella vulgaris extracts or fractions. Results showed that 95% ethanol extracts from P. vulgaris significantly inhibited PGE2 production. In further studies, fraction 2 from the 95% ethanol extract of P. vulgaris significantly reduced PGE2 production at 66 μg/ml (72% reduction). Cytotoxic-ity did not play a role in the noted reduction of PGE2 seen in either the extracts or fractions from P. vulgaris. High performance liquid chromatography analysis showed that there was 1.4 mM rosmarinic acid in 95% ethanol Prunella extract (201 mg/ml crude extract). Our results suggest that rosmarinic acid may contribute toward the anti-inflammatory activity of Prunella in a dose-response manner. Prunella might have a potential to be used as a functional food for anti-inflammatory activity.

基于主成分分析和响应面法优化夏枯草活性成分超声提取工艺

建立了夏枯草的指纹图谱,通过主成分分析法进行降维处理,确定了2个主成分,以其综合得分为评价指标,在单因素实验的基础上,采用响应面法优化夏枯草活性成分的超声提取工艺。结果表明,除提取温度外,其它因素均对综合得分有显著影响,影响大小顺序为:超声时间>乙醇体积分数>料液比;确定最佳提取工艺为:超声时间23 min、料液比1∶20(g∶mL)、乙醇体积分数65%。该方法不仅能全面考察夏枯草活性成分的提取效果,同时简化数据,具有一定的实用性,为夏枯草药材的质量控制及进一步开发利用奠定了基础。

夏枯草开音合剂治疗血瘀痰凝型声带白斑伴轻中度异型增生的临床观察

目的 观察夏枯草开音合剂治疗血瘀痰凝型声带白斑伴轻中度异型增生的临床疗效。方法 将2019年1月~2022年1月上海市中医医院的86例声带白斑患者随机分为治疗组和对照组,治疗组采用夏枯草开音合剂治疗,每次25m L,每日2次;对照组采用金嗓散结胶囊治疗,每次2粒,每日2次。治疗时间均为8周。采用症状体征积分评价临床疗效,用VHI量表、基频微扰、振幅微扰、谐噪比、MPT评价主客观嗓音功能。结果 实际完成79例,治疗组40例,对照组39例。治疗组总有效率87.5%,高于对照组66.67%(P<0.05);两组治疗后症状体征积分均下降,治疗组总积分低于对照组(P<0.05);治疗后两组嗓音功能均得到改善,治疗组基频微扰、振幅微扰低于对照组,谐噪比高于对照组(P<0.05)。结论 夏枯草开音合剂治疗血瘀痰凝型声带白斑伴轻中度异型增生疗效确切,在缓解声嘶、缩小白斑面积和声门裂宽度、改善声音粗糙嘶哑程度、以及恢复声带闭合运动方面,疗效均优于金嗓散结胶囊。

夏枯草中主要化学成分群的时空积累及抗氧化活性的动态变化研究

目的:研究不同时期不同部位夏枯草的抗氧化活性的动态变化规律及主要药用成分迷迭香酸、齐墩果酸和熊果酸的时空积累,明确抗氧化活性与化学成分的相关性,找到最佳采收期和药用部位,确保药效的最大化和资源的充分利用。方法:以不同时期、不同部位夏枯草70%乙醇提取液为研究对象,以DPPH自由基清除率为指标,进行抗氧化活性评价,并用固相萃取的方法将酚酸类成分和三萜类成分分离,采用HPLC法对不同时期的夏枯草各部位进行含量测定。同时运用SPSS26.0软件进行关联度分析。结果:不同时期不同部位的夏枯草样本中,花开期的夏枯草穗DPPH自由基清除率最高,表现出较强的抗氧化活性。运用固相萃取的方法,酚酸类成分和三萜类成分的分离度良好;夏枯草中主要的酚酸类成分迷迭香酸在夏枯草的生长发育中呈先下降后上升的趋势,其在花开期的夏枯草穗中含量最高,关联度分析显示迷迭香酸与抗氧化活性具有相关性,是发挥抗氧化活性的主要成分;三萜类成分齐墩果酸和熊果酸在夏枯草各部位的生长发育中变化趋势相同,均在花中含量最高,随着生长时期的成熟,三萜类成分在夏枯草穗中富集,其他部位的三萜类成分含量变化波动较小。结论:本研究结果确定了夏枯草抗氧化活性最高的采收期和药用部位,为夏枯草资源的充分利用和完善夏枯草质量控制标准提供了依据。