Rose leaves with virus symptoms(mosaic,ring spot and line pattern) were detected by DAS-ELISA and RT-PCR.The results showed Prunus necrotic ring spot virus(PNRSV) infection in the leaves.CP gene of PNRSV isolated from...Rose leaves with virus symptoms(mosaic,ring spot and line pattern) were detected by DAS-ELISA and RT-PCR.The results showed Prunus necrotic ring spot virus(PNRSV) infection in the leaves.CP gene of PNRSV isolated from Yunnan province was cloned and sequenced.Analysis of sequence showed that CP gene of PNRSV-yunnan contained 683 nucleotides encoding 165 aminoacids(EMBL accession number: AY684271).Nucleotide similarity of CP gene was more than 98% between PNRSV-yunnan and group I isolates,and less than 95% between PNRSV-yunnan and group II and III isolates.According to the results,PNRSV-yunnan was identified as a member of group I.展开更多
Prunus necrotic ring spot virus （PNRSV） and grapevine fanleaf virus （GFLV） were detected by fluoroimmunoassay using bacterial magnetic particles （BMPs）, and a double antibody sandwich enzyme linked immunosorbent...Prunus necrotic ring spot virus （PNRSV） and grapevine fanleaf virus （GFLV） were detected by fluoroimmunoassay using bacterial magnetic particles （BMPs）, and a double antibody sandwich enzyme linked immunosorbent assay （DAS-ELISA）. For the fluoroimmunoassay, fluorescein isothiocyanate labeled anti-PNRSV antibody or anti-GFLV antibody was conjugated onto BMPs of Magnetospirillum gryphiswaldense MSR-1. With this method, a very low minimum antigen concentration （1×10^6 dilution of the original sample concentration） could be detected. Using DAS-ELISA, the minimum antigen detection concentration was the original sample concentration. Thus, comparing these two methods, a BMP-based method could increase the sensitivity up to six orders of magnitude （10^6） higher than an ELISA-based method of detection PNRSV and GFLV.展开更多
文摘Rose leaves with virus symptoms(mosaic,ring spot and line pattern) were detected by DAS-ELISA and RT-PCR.The results showed Prunus necrotic ring spot virus(PNRSV) infection in the leaves.CP gene of PNRSV isolated from Yunnan province was cloned and sequenced.Analysis of sequence showed that CP gene of PNRSV-yunnan contained 683 nucleotides encoding 165 aminoacids(EMBL accession number: AY684271).Nucleotide similarity of CP gene was more than 98% between PNRSV-yunnan and group I isolates,and less than 95% between PNRSV-yunnan and group II and III isolates.According to the results,PNRSV-yunnan was identified as a member of group I.
文摘Prunus necrotic ring spot virus （PNRSV） and grapevine fanleaf virus （GFLV） were detected by fluoroimmunoassay using bacterial magnetic particles （BMPs）, and a double antibody sandwich enzyme linked immunosorbent assay （DAS-ELISA）. For the fluoroimmunoassay, fluorescein isothiocyanate labeled anti-PNRSV antibody or anti-GFLV antibody was conjugated onto BMPs of Magnetospirillum gryphiswaldense MSR-1. With this method, a very low minimum antigen concentration （1×10^6 dilution of the original sample concentration） could be detected. Using DAS-ELISA, the minimum antigen detection concentration was the original sample concentration. Thus, comparing these two methods, a BMP-based method could increase the sensitivity up to six orders of magnitude （10^6） higher than an ELISA-based method of detection PNRSV and GFLV.
