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炭素制品在石墨化过程中的“Puffing”
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作者 张松威 张云峰 《炭素技术》 CAS CSCD 2008年第6期48-49,58,共3页
炭素制品在石墨化过程中时常会出现导致大量裂纹废品的"Puffing"现象,严重影响了产品的质量,本文旨在探讨引起"Puffing"现象的因素、产生机理以及介绍几种在石墨制品生产过程中已经被广泛采用的抑制、减少该现象产... 炭素制品在石墨化过程中时常会出现导致大量裂纹废品的"Puffing"现象,严重影响了产品的质量,本文旨在探讨引起"Puffing"现象的因素、产生机理以及介绍几种在石墨制品生产过程中已经被广泛采用的抑制、减少该现象产生的有效措施,以提高石墨制品的质量及成品率指标。 展开更多
关键词 炭素制品 石墨化 puffing
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Temperature Simulation of Berry Slices Under Microwave Vacuum Puffing Conditions 被引量:1
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作者 Liu Hai-jun Lin Zhen +3 位作者 Zheng Xian-zhe Liu Cheng-hai Song Xiang-yu Liu Dai-ya 《Journal of Northeast Agricultural University(English Edition)》 CAS 2012年第4期75-78,共4页
In order to study the temperature simulation of berry slices in microwave vacuum puffing process, numerical simulation method was used to simulate the temperature in different time according to theoretical calculation... In order to study the temperature simulation of berry slices in microwave vacuum puffing process, numerical simulation method was used to simulate the temperature in different time according to theoretical calculation result and the deviation had been verificated throughout the experiment. The better uniform of temperature between experiment and simulation could be concluded except in the period of rapid temperature increase. The temperature reached the high point of 130.25℃ in the middle point of slices at 100 s, while the temperature was over 50℃ in external parts of slices for the reason of thermal diffusion. Conclusions could be drawn that the model was effective and numerical analysis could simulate the temperature simulation for berry leather in microwave vacuum puffing process. 展开更多
关键词 microwave vacuum puffing SIMULATION TEMPERATURE RASPBERRY
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Analysis of the Gas Puffing Performance for Improving the Repeatability of Ohmic Discharges in the SUNIST Spherical Tokamak 被引量:1
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作者 谢会乔 谭熠 +2 位作者 柯锐 王文浩 高喆 《Plasma Science and Technology》 SCIE EI CAS CSCD 2014年第8期732-737,共6页
The gas puffing performance plays a key role in repeatable discharges in the Sino- UNited Spherical Tokamak (SUNIST) experiments. In this paper, temporal evolution of the gas pressure in the vacuum vessel and the de... The gas puffing performance plays a key role in repeatable discharges in the Sino- UNited Spherical Tokamak (SUNIST) experiments. In this paper, temporal evolution of the gas pressure in the vacuum vessel and the dependence of the repeatability of plasma discharges on different timing arrangements between the gas puffing pulse and the Ohmic field have been experimentally investigated. The results show that, after a fast rising phase, the gas pressure becomes quasi-stationary. In the regime of the discharges being started up when the gas pressure has already reached the quasi-stationary state for about 37 ms, an improved repeatability of the plasma discharges is achieved. 展开更多
关键词 gas puffing performance repeatability of plasma discharges SUNIST
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The Effect of Atmospheric Oxygen on the Puffing and Bursting Phenomena during Vegetable Oils Droplets Vaporization Process for Their Use as Biofuel in Diesel Engine 被引量:1
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作者 A. S. Zongo T. Daho +6 位作者 G. Vaitilingom B. Piriou J. Valette C. Caillol B. G. Segda P. Higelin J. Koulidiati 《Energy and Power Engineering》 2018年第12期518-533,共16页
The past literature on the use of vegetable oils as fuel in diesel engine revealed that utilizing vegetable oil fuels in diesel engines may require property changes in the oil or perhaps, some minor engine modificatio... The past literature on the use of vegetable oils as fuel in diesel engine revealed that utilizing vegetable oil fuels in diesel engines may require property changes in the oil or perhaps, some minor engine modifications or operating changes. This study was conducted to search for the effect of atmospheric oxygen on the puffing and bursting phenomena that occur during vegetable oils droplet vaporization process in their use as fuel in diesel engine. The fiber-suspended droplet technique was used, and the normalized square droplet diameter as well as the temperature evolution vicinity the droplet was analyzed. The results show that puffing and bursting phenomena highly depend on oxygen. In presence of atmospheric oxygen, there is an increase of the puffing and bursting intensity and therefore the evaporation rate of the vegetable oil droplets, but in an inert environment or when the environment is oxygen-depleted puffing and bursting phenomena disappearing and make place of a series of explosions with lower magnitude. The lack of oxygen reduces the thermal degradation, polymerization and oxidation reactions and consequently the vaporization rate of vegetable oils droplets;and could therefore lead to the formation of deposits in the form of polymers. This is unsuitable for their use as a fuel in diesel engines. It can also be concluded that atmospheric oxygen has some positive effects on engine performance and emissions when operating with vegetable oil. These results help to address the challenge for the use of alternative fuels such as non-edible vegetable oils. 展开更多
关键词 puffing BURSTING Oxygen Nitrogen LINSEED Oil VAPORIZATION Engine Energy
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OEDGE modeling of plasma contamination efficiency of Ar puffing from different divertor locations in EAST
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作者 张鹏飞 张凌 +14 位作者 吴振伟 许棕 高伟 王亮 杨清泉 许吉禅 刘建斌 屈浩 刘永 黄娟 吴成瑞 侯玉梅 金钊 J D ELDER 郭后扬 《Plasma Science and Technology》 SCIE EI CAS CSCD 2018年第4期121-126,共6页
Modeling with OEDGE was carried out to assess the initial and long-term plasma contamination efficiency of Ar puffing from different divertor locations, i.e. the inner divertor, the outer divertor and the dome, in the... Modeling with OEDGE was carried out to assess the initial and long-term plasma contamination efficiency of Ar puffing from different divertor locations, i.e. the inner divertor, the outer divertor and the dome, in the EAST superconducting tokamak for typical ohmic plasma conditions. It was found that the initial Ar contamination efficiency is dependent on the local plasma conditions at the different gas puff locations. However, it quickly approaches a similar steady state value for Ar recycling efficiency >0.9. OEDGE modeling shows that the final equilibrium Ar contamination efficiency is significantly lower for the more closed lower divertor than that for the upper divertor. 展开更多
关键词 DIVERTOR Ar puffing impurity transport OEDGE EAST
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Divertor Experiments with MBI and Strong Gas Puffing on HL-2A
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作者 段旭如 丁玄同 +18 位作者 杨青巍 严龙文 姚良骅 洪文玉 宣伟民 刘德权 陈燎原 宋显明 张锦华 曹曾 崔正英 李伟 刘仪 潘宇东 潘莉 郑银甲 周艳 毛维成 刘永 《Plasma Science and Technology》 SCIE EI CAS CSCD 2006年第1期19-23,共5页
In the HL-2A 2004 experiment campaign, pulsed molecular beam injection (MBI) and strong hydrogen gas puffing under the divertor configuration were used for gas fueling. The experimental results show that the MBI of ... In the HL-2A 2004 experiment campaign, pulsed molecular beam injection (MBI) and strong hydrogen gas puffing under the divertor configuration were used for gas fueling. The experimental results show that the MBI of hydrogen can reduce the heat flux to the divertor target plate. The electron temperature measured by the Langmuir probe array decreases significantly during the injection of the molecular beam whereas the electron density increases. This indicates that the plasma pressure near the target plates tends to be constant at a new equilibrium level. In the divertor plasmas with strong hydrogen gas puffing a high plasma density up to 4.4 × 10^19 m^-3 was achieved. In addition, a phenomenon similar to the partially detached divertor regime was observed, which is being studied in open divertor tokamaks such as DIII-D to reduce the peak heat flux on the target plates near the separatrix. After a strong gas puffing the electron temperature measured on the outer divertor target plate near the separatrix decreases till below 5 eV or even lower, but that of the farther outer divertor target plate does not change obviously; and the CIII and the Ha emissions at the plasma edge decrease as expected, but the Ha emission near the Xpoint increases. These results reflects some interesting characteristics, which needs to be studied by further modeling and experiments. 展开更多
关键词 DIVERTOR molecular beam injection gas puffing plasma
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Analysis of Process of Microwave Puffing Blueberry Snacks
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作者 Zheng Xian-zhe Chen Kai-di +4 位作者 Su Xiao-lin Liu Han Xu Hao Wang Xiao-rui Han Ling-yu 《Journal of Northeast Agricultural University(English Edition)》 CAS 2018年第1期62-69,共8页
In order to reveal the mechanism of microwave puffing berry snack, a center combination experiment method was employed to explore the puffing process of blueberry snacks. Blueberry as the raw material, the microwave p... In order to reveal the mechanism of microwave puffing berry snack, a center combination experiment method was employed to explore the puffing process of blueberry snacks. Blueberry as the raw material, the microwave power, initial moisture content, puffing time and mass were employed as experimental factors. The pressure, temperature, amount of water vapor and puffing properties in terms of the volume, hardness and brittleness were employed as target parameters. The higher microwave intensity and initial moisture content resulted in the more amount of water vapor in the snacks and more holes in the appropriate puffing time. The shape and structure of the snacks tended to be stable with the removal of moisture. 展开更多
关键词 BLUEBERRY MICROWAVE puffing SNACK PRESSURE
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Simulation Study for Divertor Geometry and Gas Puffing to Handle Huge Exhaust Power in HL-2M with SOLPS5.0
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作者 崔学武 潘宇东 +2 位作者 李佳鲜 张锦华 毛瑞 《Plasma Science and Technology》 SCIE EI CAS CSCD 2013年第6期489-492,共4页
One of the critical issues to be solved for HL-2M is the power exhaust.Divertor target plate geometry strongly influences the plasma profiles by controlling the neutral recycling pattern,which in turn has a strong eff... One of the critical issues to be solved for HL-2M is the power exhaust.Divertor target plate geometry strongly influences the plasma profiles by controlling the neutral recycling pattern,which in turn has a strong effect on the symmetry and stability of the divertor plasma and finally on the whole edge region.The numerical simulation SOLPS5.0 package is used to design and explore the divertor target plates for HL-2M.We start with the choice of a proper target plate geometry,which has a smaller incidence angle in the permissible space,and then discuss the method of gas puffing to reduce the heat flux density on the target and the effects of gas puffing on the divertor plasma performance. 展开更多
关键词 SOLPS5.0 numerical simulation gas puffing
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Design of the Pumping Stations and the Gas Puffing Systems for the Superconducting Tokamak HT-7U
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作者 辜学茂 王小明 +5 位作者 秦品建 杨道文 胡建生 罗南昌 李生发 李高云 《Plasma Science and Technology》 SCIE EI CAS CSCD 2000年第6期557-563,共7页
The design of the pumping stations and the gas injection systems for the inner Chamber and the cryostat on HT-7U superconducting tokamak is described in this paper.
关键词 Design of the Pumping Stations and the Gas puffing Systems for the Superconducting Tokamak HT-7U HT
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面向物联网安全认证的RO PUF模型的研究
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作者 张学军 曹瑞浒 +2 位作者 周寅峰 谷群远 梅年松 《电子设计工程》 2024年第2期56-60,共5页
物联网设备的普及与应用极大地改变了生活方式,设备之间的身份安全问题也是使用者关注的焦点。该文基于物联网独特的体系结构,详细分析各层次中安全认证技术的必要性和使用PUF技术对物联网设备进行身份认证的可行性。该文采用一种RO PU... 物联网设备的普及与应用极大地改变了生活方式,设备之间的身份安全问题也是使用者关注的焦点。该文基于物联网独特的体系结构,详细分析各层次中安全认证技术的必要性和使用PUF技术对物联网设备进行身份认证的可行性。该文采用一种RO PUF结构,该方案通过混阶模型提高响应位的稳定性,使用可重构结构能加激励响应对的个数,使用并行架构保证结果的独立与安全。该设计通过Xilinx的Spartan6系列XC6SLX16-2CSG324 FPGA开发板进行结果测试,其可靠性可达到99.69%。 展开更多
关键词 物联网 物联网安全 身份认证技术 RO PUF
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Influences of microwave vacuum puffing conditions on anthocyanin content of raspberry snack 被引量:6
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作者 Mu Yanqiu Zhao Xinhuai +2 位作者 Liu Bingxin Liu Chenghai Zheng Xianzhe 《International Journal of Agricultural and Biological Engineering》 SCIE EI CAS 2013年第3期80-87,共8页
Microwave technology is fit for the processing of berry products,but it may affect nutrition components of berry fruit.To improve the nutritional value of the berry products,the influences of microwave vacuum puffing ... Microwave technology is fit for the processing of berry products,but it may affect nutrition components of berry fruit.To improve the nutritional value of the berry products,the influences of microwave vacuum puffing conditions on the anthocyanin content of raspberry snack were investigated using central composite experiments.Results indicated that vacuum pressure had the most significant effect on the anthocyanins of berry snack,followed by the puffing time,microwave power,and initial moisture content.The interaction between microwave power and puffing time on the anthocyanins was extremely significant.Under microwave power of 2.68 kW and the puffing time of 81.00 s,the anthocyanin content of raspberry snack was retained at high level.The results can provide some technological basis for the berry fruit products processed with high quality. 展开更多
关键词 raspberry snack microwave vacuum puffing processing condition anthocyanin content ABSORBANCE
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基于PUF技术的物联网应用技术方案
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作者 杨光 廖浩祥 《广东通信技术》 2024年第9期47-51,共5页
分析了物联网应用的各种安全问题,针对仿冒终端接入业务平台的安全风险,设计了一种基于PUF安全技术的IPv6地址的生成和验证方法,实现了平台对终端/卡进行身份认证的安全方案并定义了详细的交互流程,有助于业务平台防范非法接入攻击、保... 分析了物联网应用的各种安全问题,针对仿冒终端接入业务平台的安全风险,设计了一种基于PUF安全技术的IPv6地址的生成和验证方法,实现了平台对终端/卡进行身份认证的安全方案并定义了详细的交互流程,有助于业务平台防范非法接入攻击、保障平台和业务数据的安全。 展开更多
关键词 PUF IPV6 DHC Pv6 密钥协商 安全 身份认证
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基于阻变存储器的物理不可克隆函数设计
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作者 冯平 廖文丽 +2 位作者 左石凯 陈铖颖 黄渝斐 《半导体技术》 CAS 北大核心 2024年第4期341-349,共9页
物理不可克隆函数(PUF)将集成电路制造过程中产生的工艺变化作为一种安全原语,已被广泛应用于硬件安全领域,特别是身份认证和密钥存储。提出了一种基于阻变存储器(RRAM)阵列的PUF优化设计,采用2T2R差分存储结构,并利用阵列中RRAM单元的... 物理不可克隆函数(PUF)将集成电路制造过程中产生的工艺变化作为一种安全原语,已被广泛应用于硬件安全领域,特别是身份认证和密钥存储。提出了一种基于阻变存储器(RRAM)阵列的PUF优化设计,采用2T2R差分存储结构,并利用阵列中RRAM单元的阻值变化产生PUF的随机性,以实现更高安全级别所需的大量激励-响应对(CRP)。RRAM PUF的存储单元基于28 nm工艺实现,其面积仅为0.125μm~2,相比传统PUF存储单元面积开销减小,在入侵和侧信道攻击方面具有更好的鲁棒性。实验数据表明,RRAM PUF唯一性达到了约49.78%,片内汉明距离为0%,一致性良好,具有较好的随机性。 展开更多
关键词 硬件安全 阻变存储器(RRAM) 物理不可克隆函数(PUF) 激励-响应对(CRP) 灵敏放大器
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Effects of Fermented Puffed Feather Meal on Growth Performance,Serum Biochemical Indices,Meat Quality,and Intestinal Microbiota in Broilers
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作者 Lin Yu Chen Zhihui +9 位作者 Wu Hongzhi Ji Fengjie Liu Yang Li Jianing Yu Xilong Dou Renkai Yuan Hua Yang Qinghui Li Zhongyu Xu Liangmei 《Journal of Northeast Agricultural University(English Edition)》 CAS 2024年第3期42-58,共17页
This study aimed to investigate the effects of fermented puffed feather meal(FPFM)on growth performance,serum biochemical indices,meat quality,and intestinal microbiota in Arbor Acres(AA)broilers.A single-factor desig... This study aimed to investigate the effects of fermented puffed feather meal(FPFM)on growth performance,serum biochemical indices,meat quality,and intestinal microbiota in Arbor Acres(AA)broilers.A single-factor design was adopted,and four treatments were administered with five replicates to 240 one-day-old AA broilers.The control group(group A)received a basal diet,while the experimental groups received a basal diet plus 33%(group B),67%(group C)and 100%(group D)FPFM,respectively.Compared with group A,(1)the average daily gain(ADG)in group C decreased(P<0.05),and the feed conversion ratio(FCR)in group D increased(P<0.05);(2)the level of serum urea nitrogen in treatment groups decreased(P<0.05),and the levels of triglyceride,high density lipoprotein,low density lipoprotein,cholesterol,and glucose contents in group D increased(P<0.05)at day 21;(3)the serum immunoglobulin M and immunoglobulin G in group B and the immunoglobulin A in group C increased(P<0.05)at day 21,and the serum immunoglobulin M and immunoglobulin G in group D decreased(P<0.05)at day 42;(4)the share force of breast muscle and thigh muscle in group D increased(P<0.05);(5)the villus height to crypt depth ratio in the jejunum of group B increased(P<0.05)at day 21,and the villus height in group C and D increased(P<0.05)at day 42;(6)the proteobacteria counts in the cecum digesta in treatment groups decreased(P<0.05)at day 21.The basal diet supplemented with 33%FPFM promoted protein metabolism,enhanced immunity and improved meat quality,promoted the digestion and absorption of nutrients,increased intestinal microbial diversity,and improved the content of beneficial bacteria without affecting the growth performance,it was possible to be used as a good substitute for fish meal. 展开更多
关键词 fermented puffed feather meal(FPFM) growth performance serum biochemical indices meat quality intestinal microbiota
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嵌入式系统中的内生存储器PUF研究综述
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作者 陈帅 蔡敏 +1 位作者 杨志勇 曾集丰 《集成电路与嵌入式系统》 2024年第9期7-16,共10页
物理不可克隆函数(Physical Unclonable Functions,PUF)可视为一种“芯片指纹”,具有轻量级、不可预测、难以克隆等特性,已经成为物联网硬件安全机制的重要组成部分。然而,传统PUF的实现需要在硬件层面增加FPGA或专用集成电路,会增加硬... 物理不可克隆函数(Physical Unclonable Functions,PUF)可视为一种“芯片指纹”,具有轻量级、不可预测、难以克隆等特性,已经成为物联网硬件安全机制的重要组成部分。然而,传统PUF的实现需要在硬件层面增加FPGA或专用集成电路,会增加硬件成本。对于数以亿计的现有物联网嵌入式设备,难以通过硬件改造来增加PUF安全属性。因此,从现有设备的硬件结构中挖掘固有PUF(Intrinsic PUF,IPUF)成为PUF研究与产业化的关键组成部分。本工作将对已有的IPUF,尤其是基于存储器的IPUF(Memroy based Intrinsic PUF,MIPUF)相关工作进行系统综述,并针对IPUF在现有系统中的可实现性及未来的发展方向进行讨论,以推动该技术的工程应用。 展开更多
关键词 PUF SRAM DRAM FLASH
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基于PUF的无人机网络轻量级身份认证方案
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作者 朱庆森 宋翔飞 何智旺 《软件导刊》 2024年第6期114-120,共7页
在许多应用场景中,无人机收集的数据具有敏感性和私密性,必须考虑其安全性和隐私性。为此,提出基于PUF的无人机身份认证与密钥协商方案。该方案可以通过一次会话生成两架无人机之间的会话密钥,以及每架无人机与地面站之间的会话密钥,实... 在许多应用场景中,无人机收集的数据具有敏感性和私密性,必须考虑其安全性和隐私性。为此,提出基于PUF的无人机身份认证与密钥协商方案。该方案可以通过一次会话生成两架无人机之间的会话密钥,以及每架无人机与地面站之间的会话密钥,实现了多项安全功能。与现有无人机认证协商方案的性能比较结果表明,该方案在计算开销方面减少了17%,在平均通信开销方面减少了12%。该方案适用于实时性强、计算资源不足的无人机互联网。 展开更多
关键词 无人机 PUF 身份认证 密钥协商 安全性证明
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基于Xilinx Virtex-7系列FPGA器件配置存储空间PUF可行性探索
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作者 郭俊杰 王婧 谢达 《电子与封装》 2024年第9期71-79,共9页
在静态随机存取存储器(SRAM)型现场可编程门阵列(FPGA)电路中,当电源启动并进行初始化时,SRAM单元通常会经历一个全面复位的过程。复位过程会导致SRAM单元内部的电荷分布形成一种特有的模式。提出了一种新的方法,利用配置内存中尚未使... 在静态随机存取存储器(SRAM)型现场可编程门阵列(FPGA)电路中,当电源启动并进行初始化时,SRAM单元通常会经历一个全面复位的过程。复位过程会导致SRAM单元内部的电荷分布形成一种特有的模式。提出了一种新的方法,利用配置内存中尚未使用的部分来识别电路,对8个Xilinx Virtex-7 FPGA进行总计200 000次的测量,评估了这种方法在同一电路中的一致性和在不同电路之间的差异性,以及随温度变化时和随时间老化后的稳定性。研究结果显示,SRAM物理不可克隆函数(PUF)能够有效地区分不同的FPGA电路。 展开更多
关键词 SRAM PUF FPGA 配置内存空间
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Puffing Their Best Feet Forward
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作者 YAN WEI 《Beijing Review》 2008年第21期32-33,共2页
Foreign diplomats in Beijing hit the road in a solidarity fun run for the Olympics It was a day out of the office for Philippe le Gall.Clad in a baggy white T-shirt and black miming shorts,the Seychelles’ ambassador ... Foreign diplomats in Beijing hit the road in a solidarity fun run for the Olympics It was a day out of the office for Philippe le Gall.Clad in a baggy white T-shirt and black miming shorts,the Seychelles’ ambassador to China looked ready for action. Le Gall was running a 1-kin race in a villa complex in northeast Beijing,where the Slovenian Embassy is located.The race, hosted by the embassy on April 26,104 days before the opening of the Beijing Olympics, attracted diplomats from about 50 countries. Its theme was"diplomats running for peace and friendship." 展开更多
关键词 puffing Their Best Feet Forward
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Puffing quaternary FexCoyNi1-x-yP nanoarray via kinetically controlled alkaline etching for robust overall water splitting 被引量:9
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作者 Haiqing Wang Xiaowei Zhang +5 位作者 Jingang Wang Huiling Liu Shuxian Hu Weijia Zhou Hong Liu Xun Wang 《Science China Materials》 SCIE EI CSCD 2020年第6期1054-1064,共11页
Designing and constructing bifunctional electrocatalysts with high efficiency,high stability and low cost for overall water splitting to produce clean hydrogen fuel is attractive but highly challenging.Here we constru... Designing and constructing bifunctional electrocatalysts with high efficiency,high stability and low cost for overall water splitting to produce clean hydrogen fuel is attractive but highly challenging.Here we constructed puffed quaternary FexCoyNi1-x-yP nanoarrays as bifunctional electrodes for robust overall water splitting.The iron was used as the modulator to manipulate the electron density of NiCoP nanoarray,which could increase the positive charges of metal(Ni and Co)and P sites.The resultant electronic structure of FexCoyNi1-x-yP was supposed to balance the adsorption and desorption of H and accelerate the oxygen evolution reaction(OER)kinetics.Moreover,the morphological structure of FexCoyNi1-x-yP was modulated through the kinetically controlled alkaline etching by using the amphoteric features of initial FeCoNi hydroxide nanowires.The resultant puffed structure has rich porosity,cavity and defects,which benefit the exposure of more active sites and the transport of mass/charge.As a result,the cell integrated with the puffed quaternary FexCoyNi1-x-yP nanoarrays as both the cathode and anode only requires the overpotentials of 25 and 230 mV for hydrogen evolution reaction(HER)and OER at the current density of 10 mA cm^-2 in alkaline media and a cell voltage of 1.48 V to drive the overall water splitting.Moreover,the puffed FexCoyNi1-x-yP demonstrates remarkable durability for continuous electrolysis even at a large current density of 240 mA cm^-2. 展开更多
关键词 puffed nanoarray morphology control alkaline etching ELECTROCATALYSIS water splitting
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基于多组学数据分析PUF家族基因在肝癌中的表达及其临床意义
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作者 唐超峰 柳科军 +1 位作者 陈本栋 卜阳 《宁夏医科大学学报》 2023年第7期668-675,共8页
目的 探讨PUF家族基因(PUM1、PUM2、PUM3)作为肝癌的潜在预后标志物及其可能的分子调控机制。方法 TCGA数据库分析PUM1、PUM2、PUM3在正常肝组织、癌旁组织和肝癌组织中的表达及差异;Kaplan-Meier曲线分析PUM1、PUM2、PUM3表达与肿瘤患... 目的 探讨PUF家族基因(PUM1、PUM2、PUM3)作为肝癌的潜在预后标志物及其可能的分子调控机制。方法 TCGA数据库分析PUM1、PUM2、PUM3在正常肝组织、癌旁组织和肝癌组织中的表达及差异;Kaplan-Meier曲线分析PUM1、PUM2、PUM3表达与肿瘤患者预后;c Bio Portal数据库分析PUM1、PUM2、PUM3在肝癌中的突变情况;String网站及Cytoscape软件构建PUM1、PUM2、PUM3共表达基因的蛋白互作网络;GO/KEGG对PUM1、PUM2、PUM3共表达基因进行功能富集分析;TIMER 2.0数据库分析PUM1、PUM2、PUM3与肿瘤免疫细胞浸润的相关性。结果 PUM1、PUM2、PUM3在肝癌组织的表达高于癌旁组织及正常肝组织(P均<0.001);PUM1、PUM2、PUM3阳性蛋白表达主要集中于细胞核或细胞质;PUM1表达与患者年龄、BMI及组织学分级相关(P均<0.05);PUM2表达与患者性别、年龄及病理分期相关(P均<0.05);PUM3表达与肿瘤T分期及病理分期相关(P均<0.05);PUM1、PUM2、PUM3高表达的肝癌患者预后差(P<0.05);PUMs共表达基因主要富集于RNA剪切、P53类介质对信号转导的正向调节作用、剪接体组装、转录因子复合体、钙粘蛋白(Ecadherin)结合、P53结合位点及细胞周期等通路。同时PUM1、PUM2、PUM3表达与辅助性T细胞、中枢记忆性T细胞、辅助性T细胞2及嗜酸性粒细胞浸润水平呈正相关关系。结论 PUM1、PUM2、PUM3在肝癌发生发展过程中发挥重要作用,可作为潜在的分子治疗靶点和预后评价标志物。 展开更多
关键词 肝细胞癌 PUF家族基因 预后 免疫浸润 生物信息学
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