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中枢神经系统5-羟色胺受体显像剂WAY类似物的研究进展 被引量:4
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作者 刘飞 贺佑丰 罗志福 《同位素》 CAS 2001年第1期41-49,共9页
阐述了 WAY类似物在中枢神经系统 5-羟色胺 (5- HT)受体显像剂方面的研究状况 ,介绍了这类化合物在中枢神经系统 5- HT受体显像剂方面的优越性 ,并对 99Tcm 标记
关键词 中枢神经系统 受体显像剂 核医学 锝99标记物 5-羟色胺 抗结剂 way类似物
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α-Synuclein与14-3-3蛋白在体外分子间相互作用的鉴定 被引量:2
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作者 黄银霞 韩俊 +5 位作者 韩露 董辰方 高晨 王小凡 周伟 董小平 《中国生物化学与分子生物学报》 CAS CSCD 北大核心 2007年第6期469-473,共5页
帕金森病(Parkinson's disease,PD)涉及两种蛋白——α-synuclein蛋白(SNCP)与14-3-3蛋白.通过重组,将这两种蛋白在大肠杆菌DH5α中表达,通过谷胱甘肽-Sepharose4B亲和层析将其纯化,得到GST-14-3-3蛋白;利用凝血酶对纯化的融合蛋白G... 帕金森病(Parkinson's disease,PD)涉及两种蛋白——α-synuclein蛋白(SNCP)与14-3-3蛋白.通过重组,将这两种蛋白在大肠杆菌DH5α中表达,通过谷胱甘肽-Sepharose4B亲和层析将其纯化,得到GST-14-3-3蛋白;利用凝血酶对纯化的融合蛋白GST-SNCP切割,再经谷胱甘肽-Sepharose4B亲和层析获得SNCP.通过免疫共沉淀、GSTpull down和ELISA等技术,证明SNCP能够与14-3-3蛋白结合;为了进一步证明SNCP也与在脑组织中的天然14-3-3蛋白发生作用,利用His pull down方法进行实验.结果证明,SNCP能够和脑组织中的14-3-3蛋白发生相互作用.这些结果从分子水平提供了SNCP与14-3-3蛋白相互作用的实验证据,为进一步了解SNCP的结构和功能,及其在中枢神经系统退行性疾病的作用提供了必要的实验基础. 展开更多
关键词 退行性疾病 免疫共沉淀 pull down 蛋白相互作用 Α-SYNUCLEIN 14-3-3蛋白
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Optical and near-infrared photometric study of NGC 6724
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作者 Reda Bendary Ashraf Tadross +2 位作者 Priya Hasan Anas Osman Ahmed Essam 《Research in Astronomy and Astrophysics》 SCIE CAS CSCD 2018年第2期37-42,共6页
BVRI CCD photometry of the poorly studied open cluster NGC 6724 has been carried out down to a limiting magnitude of V-20 mag. The stars of the cluster have been observed using the Newtonian focus (f/4.84) of the 74... BVRI CCD photometry of the poorly studied open cluster NGC 6724 has been carried out down to a limiting magnitude of V-20 mag. The stars of the cluster have been observed using the Newtonian focus (f/4.84) of the 74-inch telescope at Kottamia Astronomical Observatory in Egypt. Also, the 2MASS - JHK system is used to confirm the results we obtained. The main photometric parameters have been estimated for the present object; the diameter is found to be 6 arcmin, the distance is 15304-60pc from the Sun and the age is 900+50Myr. The optical reddening E(B - V) = 0.65 mag, while the infrared reddening is E(J - H) = 0.20 mag. The slope of the mass function distribution and the relaxation time estimations indicate that cluster NGC 6724 is dynamically relaxed. 展开更多
关键词 Milky way -- open clusters and associations: individual (NGC 6724) - optical photom-etry - IR photometry - color-magnitude diagrams
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两种新型^99mTc配合物的制备和初步生物分布研究
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作者 王凤龙 王学斌 张现忠 《北京师范大学学报(自然科学版)》 CAS CSCD 北大核心 2009年第2期172-175,共4页
为了制备适于99mTc标记的5-HT1A受体显像剂,将WAY100635中具有生物活性的1-(2-甲氧基苯基)哌嗪(MPP)部分与双功能联接剂1,4,8,11-四氮杂环十四烷(cyclam)相连接制得MPPC配体,再分别利用99mTcN核和99mTcO2核进行标记,得到新型电中性的99m... 为了制备适于99mTc标记的5-HT1A受体显像剂,将WAY100635中具有生物活性的1-(2-甲氧基苯基)哌嗪(MPP)部分与双功能联接剂1,4,8,11-四氮杂环十四烷(cyclam)相连接制得MPPC配体,再分别利用99mTcN核和99mTcO2核进行标记,得到新型电中性的99mTcN-MPPC和正电荷的99mTcO2-MPPC配合物,2种配合物经薄层色谱(TLC)和高效液相色谱(HPLC)鉴定,放射化学纯度都在95%以上.这提供了1种将99mTc连接到WAY100635药效基团的新方法.2种配合物均呈水溶性,并可在室温下稳定存在6h以上.小鼠生物分布结果表明:2种配合物在脑中均有一定的摄取和滞留,且99mTcN-MPPC的初始脑摄取明显高于99mTcO2-MPPC. 展开更多
关键词 5-HT1A受体 1 4 8 11-四氮杂环十四烷 MPPC配体 way100635
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Effects of lead,cadmium,mercury chlorides and aquatic environmental samples on the growth of Escherichia coli 被引量:2
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作者 Xu Chao(Department of Environmental Science and Engineering , Nanjing University , Nanjing 210093 , China)Yuan Jing(Department of Plants , Soils , and Biometeorology , Utah State University , Logan , Utah 84322-4820, U. S. A)Xu Heping(Environmental Monito 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 1995年第4期455-460,共6页
Effectsoflead,cadmium,mercurychloridesandaquaticenvironmentalsamplesonthegrowthofEscherichiacoliXuChao(Depar... Effectsoflead,cadmium,mercurychloridesandaquaticenvironmentalsamplesonthegrowthofEscherichiacoliXuChao(DepartmentofEnvironmen... 展开更多
关键词 heavy metal ions Escherithia coli two - way growth effects.
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基于Push-Pull调制的分布反馈激光器带宽研究
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作者 周宁 左成亮 +4 位作者 林泽锟 兰明文 董智星 柯程 齐俊秋 《激光与光电子学进展》 CSCD 北大核心 2018年第6期287-294,共8页
为提高分布式反馈(DFB)激光器小信号曲线的通带平坦度和3dB带宽,利用一维行波模型,研究了一阶和二阶光栅结构下基于push-pull调制原理的DFB激光器,分析了器件参数(如光栅耦合系数、腔长、电极比例等)与激光器频率响应的关系。其中,仿真... 为提高分布式反馈(DFB)激光器小信号曲线的通带平坦度和3dB带宽,利用一维行波模型,研究了一阶和二阶光栅结构下基于push-pull调制原理的DFB激光器,分析了器件参数(如光栅耦合系数、腔长、电极比例等)与激光器频率响应的关系。其中,仿真模型所用的参数是与实际器件的光电特性对照后提取出来的参数。仿真结果表明:对于一阶光栅结构的DFB激光器,在合适的光栅耦合系数、腔长和电极比例下,push-pull调制的带宽比单电极直接调制的带宽大12GHz;对于二阶光栅结构的DFB激光器,在合适的参数下,push-pull调制的带宽比单电极直接调制的带宽大40GHz;并且,一阶和二阶光栅结构的push-pull调制激光器的通带平坦度都优于普通的直接调制激光器。同时,实验结果也表明,即使对于普通的有源区设计,采用push-pull调制技术后,其带宽也会大幅提升。 展开更多
关键词 激光器 带宽 push—pull 光子-光子谐振
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The LEGUE disk targets for LAMOST's pilot survey
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作者 Li Chen Jin-Liang Hou +18 位作者 Jin-Cheng Yu Chao Liu Li-Cai Deng Heidi Jo Newberg Jeffrey L. Carlin Fan Yang Yue-Yang Zhang Shi-Yin Shen Hao-Tong Zhang Jian-Jun Chen Yu-Qin Chen Norbert Christlieb Zhan-Wen Han Hsu-Tai Lee Xiao-Wei Liu Kai-Ke Pan Jian-Rong Shi Hong-Chi Wang Zi Zhu 《Research in Astronomy and Astrophysics》 SCIE CAS CSCD 2012年第7期805-812,共8页
We describe the target selection algorithm for the low latitude disk por- tion of the LAMOST Pilot Survey, which aims to test systems in preparation for the LAMOST spectroscopic survey. We use the PPMXL astrometric ca... We describe the target selection algorithm for the low latitude disk por- tion of the LAMOST Pilot Survey, which aims to test systems in preparation for the LAMOST spectroscopic survey. We use the PPMXL astrometric catalog, which provides positions, proper motions, B/R/I magnitudes (mostly) from USNO-B and d/H/Ks from the Two Micron All Sky Survey (2MASS) as well. We chose eight plates along the Galactic plane, in the region 0° 〈 α° 〈 67° and 42° 〈 δ 〈 59°, which cover 22 known open clusters with a range of ages. Adjacent plates may have some small overlapping area. Each plate covers an area of 2.5° in radius, with its cen- tral star (for the Shack-Hartmann guider) brighter than 8th magnitude. For each plate, we create an input catalog in the magnitude range 11.3 〈 Imag 〈 16.3 and Bmag available from PPMXL. The stars are selected to satisfy the requirements of the fiber positioning system and have a uniform distribution in the I vs. B - I color-magnitude diagram. Our final input catalog consists of 12 000 objects on each of eight plates that are observable during the winter observing season from the Xinglong Station of the National Astronomical Observatory of China. 展开更多
关键词 disk: Milky way - stars: spectroscopy -- survey: pilot
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5-羟色胺受体对小鼠异氟烷、七氟烷遗忘作用的影响 被引量:1
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作者 马丽丽 许鹏程 戴体俊 《国际麻醉学与复苏杂志》 CAS 2009年第6期501-503,共3页
目的探讨5-羟色胺(5-hydmxytry ptamine,5-HT)受体与吸入麻醉药异氟烷、七氟烷遗忘作用的关系。方法建立小鼠腹腔注射异氟烷、七氟烷遗忘模型,在跳台、避暗实验中观察和记录不同剂量5-HT受体拮抗剂(WAY100635)侧脑室注射对跳台潜... 目的探讨5-羟色胺(5-hydmxytry ptamine,5-HT)受体与吸入麻醉药异氟烷、七氟烷遗忘作用的关系。方法建立小鼠腹腔注射异氟烷、七氟烷遗忘模型,在跳台、避暗实验中观察和记录不同剂量5-HT受体拮抗剂(WAY100635)侧脑室注射对跳台潜伏期、步入潜伏期、错误次数的影响。结果侧脑室注射5-HT受体拮抗剂可延长异氟烷、七氟烷所致记忆障碍小鼠的跳台潜伏期(P〈O.05)、步入潜伏期(P〈0.05),减少错误次数(P〈0.05)。结论5-HT受体介导了异氟烷、七氟烷的遗忘作用。 展开更多
关键词 5-羟色胺受体 遗忘 跳台法 避暗法 way100635
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Size distribution,directional source contributions and pollution status of PM from Chengdu,China during a long-term sampling campaign 被引量:1
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作者 Guo-Liang Shi Ying-Ze Tian +5 位作者 Tong Ma Dan-Lin Song Lai-Dong Zhou Bo Han Yin-Chang Feng Armistead G.Russell 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2017年第6期1-11,共11页
Long-term and synchronous monitoring of PMIo and PM2.s was conducted in Chengdu in China from 2007 to 2013. The levels, variations, compositions and size distributions were investigated. The sources were quantified by... Long-term and synchronous monitoring of PMIo and PM2.s was conducted in Chengdu in China from 2007 to 2013. The levels, variations, compositions and size distributions were investigated. The sources were quantified by two-way and three-way receptor models (PMF2, ME2-2way and ME2-3way), Consistent results were found: the primary source categories contributed 63.4% (PMF2), 64.8% (ME2-2way) and 66.8% (ME2-Bway) to PMIo, and contributed 60.9% (PMF2), 65.5% (ME2-2way) and 61.0% (ME2-3way) to PM2.s. Secondary sources contributed 31.8% (PMF2), 32.9% (ME2-2way) and 31.7% (ME2-3way) to PMIo, and 35.0% (PMF2), 33.8% (ME2-2way) and 36.0% (ME2-3way) to PM2.s. The size distribution of source categories was estimated better by the ME2-3way method. The three-way model can simultaneously consider chemical species, temporal variability and PM sizes, while a two-way model independently computes datasets of different sizes. A method called source directional apportionment (SDA) was employed to quantify the contributions from various directions for each source category. Crustal dust from east-north-east (ENE) contributed the highest to both PM^o (12.7%) and PMzs (9.7%) in Chengdu, followed by the crustal dust from south-east (SE) for PMao (9.8%) and secondary nitrate & secondary organic carbon from ENE for PMzs (9.6%). Source contributions from different directions are associated with meteorological conditions, source locations and emission patterns during the sampling period. These findings and methods provide useful tools to better understand PM pollution status and tn dovolon offoctive nolhltion control gtrateMeg. 展开更多
关键词 PM10 PM2.5 PMF2 ME2-3way Size distribution Source directional apportionment
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重组人死亡受体6胞外结构域的制备及与淀粉样前体蛋白N端片段相互作用鉴定
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作者 牛纯青 高香 +4 位作者 罗金华 李伟 刘秋萍 陈韵 刘堰 《中国生物工程杂志》 CAS CSCD 北大核心 2016年第2期1-6,共6页
人淀粉样前体蛋白氨基端切割片段(a cleaved amino-terminal fragment of amyloid precursor protein,N-APP)结合死亡受体6(Death receptor-6,DR6),会触发神经元和轴突依赖天冬氨酸特异性半胱氨酸蛋白酶的自我毁灭过程,从而导致阿尔茨... 人淀粉样前体蛋白氨基端切割片段(a cleaved amino-terminal fragment of amyloid precursor protein,N-APP)结合死亡受体6(Death receptor-6,DR6),会触发神经元和轴突依赖天冬氨酸特异性半胱氨酸蛋白酶的自我毁灭过程,从而导致阿尔茨海默病(Alzheimer’s disease,AD)的发生。为了在分子水平上研究N-APP-DR6诱导神经元退化途径,制备大量纯化的重组DR6胞外结构域以及鉴定NAPP和DR6胞外结构域的结合位点是关键。从甲醇毕赤酵母中成功表达并纯化了重组DR6胞外域(aa42-349),得率高达90 mg/L。为了验证DR6和NAPP的相互作用关系,构建谷胱甘肽转移酶-死亡受体6(GST-DR6)(aa42-349)融合蛋白原核表达质粒,转化大肠杆菌(Escherichia coli,E.coli)BL21,表达融合蛋白GST-DR6(aa42-349),同时纯化得到DR6的配体NAPP,GST pull-down结果显示DR6与NAPP能够在细胞外发生相互作用。 展开更多
关键词 阿尔茨海默病 死亡受体-6 淀粉样前体蛋白 毕赤酵母 GST pull down
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