[Objective] The paper was to explore the cause of disease with symptoms of swelling joint, blanc-loose stool and few deaths, so as to provide suggestions and effective measures for diagnosis and control of the disease...[Objective] The paper was to explore the cause of disease with symptoms of swelling joint, blanc-loose stool and few deaths, so as to provide suggestions and effective measures for diagnosis and control of the disease in chicken farm. [Method] The diseased chick samples were collected for bacteriological examination. Through bacterial isolation culture, PCR identification, biochemical test and animal test, 5 strains of S. pullorum were identified. [Result] When the isolates were inoculated into SPF chicks, the same disease cases with natural infection could be reproduced. [Conclusion] S. pullorum is the major cause of the disease in chicken farm.展开更多
Background:Salmonella pullorum is one of the most harmful pathogens to avian species.Magnolol and honokiol,natural compounds extracted from Magnolia officinalis,exerts anti-inflammatory,anti-oxidant and antibacterial ...Background:Salmonella pullorum is one of the most harmful pathogens to avian species.Magnolol and honokiol,natural compounds extracted from Magnolia officinalis,exerts anti-inflammatory,anti-oxidant and antibacterial activities.This study was conducted to evaluate the effects of dietary supplemental magnolol and honokiol in broilers infected with S.pullorum.A total of 360 one-day-old broilers were selected and randomly divided into four groups with six replicates:the negative control group(CTL),S.pullorum-infected group(SP),and the S.pulloruminfected group supplemented with 300 mg/kg honokiol(SPH)or magnolol(SPM).Results:The results showed that challenging with S.pullorum impaired growth performance in broilers,as indicated by the observed decreases in body weight(P<0.05)and average daily gains(P<0.05),along with increased spleen(P<0.01)and bursa of Fabricus weights(P<0.05),serum globulin contents,and the decreased intestine villus height and villus/crypt ratios(P<0.05).Notably,supplemental magnolol and honokiol attenuated these adverse changes,and the effects of magnolol were better than those of honokiol.Therefore,we performed RNA-Seq in ileum tissues and 16S rRNA gene sequencing of ileum bacteria.Our analysis revealed that magnolol increased the α-diversity(observed species,Chao1,ACE,and PD whole tree)and β-diversity of the ileum bacteria(P<0.05).In addition,magnolol supplementation increased the abundance of Lactobacillus(P<0.01)and decreased unidentified Cyanobacteria(P<0.05)both at d 14 and d 21.Further study confirmed that differentially expressed genes induced by magnolol and honokiol supplementation enriched in cytokine-cytokine receptor interactions,in the intestinal immune network for IgA production,and in the cell adhesion molecule pathways.Conclusions:Supplemental magnolol and honokiol alleviated S.pullorum-induced impairments in growth performance,and the effect of magnolol was better than that of honokiol,which could be partially due to magnolol’s ability to improve the intestinal microbial and mucosal barrier.展开更多
In this study, a safety enhanced Salmonella Pullorum （S. Pullorum） ghost was constructed using an antimicrobial peptide gene, and evaluated for its potential as a Pullorum disease （PD） vaccine candidate. The antim...In this study, a safety enhanced Salmonella Pullorum （S. Pullorum） ghost was constructed using an antimicrobial peptide gene, and evaluated for its potential as a Pullorum disease （PD） vaccine candidate. The antimicrobial peptide SMAP29 was co-expressed with lysis gene E to generate S. Pullorum ghosts. No viable bacteria were detectable either in the fermentation culture after induction of gene E- and SMAP29-mediated lysis for 24 h or in the lyophilized ghost products. Specific-pathogen- free （SPF） chicks were intraperitoneally immunized with ghosts at day 7 of age and no mortality, clinical symptoms or signs of PD such as anorexia, depression and diarrhea were observed. On challenge with a virulent S. Pullorum strain at 4 wk post-immunization, a comparatively higher level of protection was observed in the S. Pullorum ghost immunized chickens with a minimum of pathological lesions and bacterial loads compared to the birds in inactivated vaccine groups. In addition, immunization with the S. Pullorum ghosts induced a potent systemic IgG response and was associated with significantly increased levels of cytokine IFN-y and IL-4 and relative percentages of CD4＋ and CD8＋ T lymphocytes. Our results indicate that SMAP29 can be employed as a new secondary lethal protein to enhance the safety of bacterial ghosts, and to prepare a non-living bacterial vaccine candidate that can prevent PD in chickens.展开更多
The isolation and culture of conventional detection method of salmonella can not meet the testing requirements of quick and easy detection at the grassroots level. In this study, we prepare the fluorescent nanoparticl...The isolation and culture of conventional detection method of salmonella can not meet the testing requirements of quick and easy detection at the grassroots level. In this study, we prepare the fluorescent nanoparticles as a marker, covalently conjugate with monoclonal antibodies of Salmonella pullorum. The whole Salmonella pullorum antigen and goat anti-mouse antibody sprayed on the nitrocellulose membranes are used as test line and control line. The fluorescence nanoparticles immune based lateral flow strips are made according to the principle of antigen-antibody immune response. The test strips may interpret results within 30 min. The results of the salmonella A, S. agona, S. chester and S. arechavaleta are positive, including, S. agona for weakly positive. After analysis, it is found that in addition to the salmonella of group A, the other positive salmonella are in group B. But it is negative of S. derby, S. rissen, and other 6 kinds of salmonella, with good specificity. The fluorescence nanoparticles immune based lateral flow strips are a little of sample can be detected fast, easily, inexpensive, easy to universal without professional technical personnel detection method. It provides a new detection method for the detections of Salmonella pullorum.展开更多
supported by the Earmarked Fund for the Modern Agroindustry Technology Research System, China （CARS-41）;the National High Technology Research and Development Program of China （2011AA100301）
BACKGROUND： For many years, yeast cell walls （YCW） and mannan oligosaccharides （MOS） have been used as alternatives to antibiotics and health feed additives to enhance the growth performance and health of food an...BACKGROUND： For many years, yeast cell walls （YCW） and mannan oligosaccharides （MOS） have been used as alternatives to antibiotics and health feed additives to enhance the growth performance and health of food animals. In the present study, the inhibitory effects of YCW and MOS on the adhesion of enteropathogenic bacteria to intestinal epithelial cells were tested. METHODS： YCW and MOS were extracted from Saccharomyces cerevisiae （XM 0315）, and the morphology of YCW and MOS bound to pathogenic bacteria was observed by scanning electron microscopy （SEM）. Real-time fluorescent quantitative PCR was used to quantitatively analyze the effects of YCW and MOS on the adhesion of Escherichia coli （CVCC3367） and Salmonella pullorum （CVCC520） to Caco-2 cells. RESULTS： The results showed that YCW inhibited E. coli and S. pullorum binding to Caco-2 cells by 95% and 74%, respectively, whereas MOS prevented E. coli and S. pullorum binding by 67% and 50%, respectively. CONCLUSIONS： These data suggest that YCW has a stronger ability than MOS to inhibit pathogenic bacteria from adhering to Caco-2 cells in vitro.展开更多
基金Supported by National Natural Science Foundation of China (30871867)Youth Fund Project of Beijing Academy of Agriculture and Forestry Science (QNJJ201012)~~
文摘[Objective] The paper was to explore the cause of disease with symptoms of swelling joint, blanc-loose stool and few deaths, so as to provide suggestions and effective measures for diagnosis and control of the disease in chicken farm. [Method] The diseased chick samples were collected for bacteriological examination. Through bacterial isolation culture, PCR identification, biochemical test and animal test, 5 strains of S. pullorum were identified. [Result] When the isolates were inoculated into SPF chicks, the same disease cases with natural infection could be reproduced. [Conclusion] S. pullorum is the major cause of the disease in chicken farm.
基金supported by the project of Hubei innovation center of agricultural science and technology(grant number 2016-620-000-001-028)National Natural Science Foundation of China(31702309)the Youth Fund of Hubei Academy of Agricultural Sciences(2019NKYJJ03).
文摘Background:Salmonella pullorum is one of the most harmful pathogens to avian species.Magnolol and honokiol,natural compounds extracted from Magnolia officinalis,exerts anti-inflammatory,anti-oxidant and antibacterial activities.This study was conducted to evaluate the effects of dietary supplemental magnolol and honokiol in broilers infected with S.pullorum.A total of 360 one-day-old broilers were selected and randomly divided into four groups with six replicates:the negative control group(CTL),S.pullorum-infected group(SP),and the S.pulloruminfected group supplemented with 300 mg/kg honokiol(SPH)or magnolol(SPM).Results:The results showed that challenging with S.pullorum impaired growth performance in broilers,as indicated by the observed decreases in body weight(P<0.05)and average daily gains(P<0.05),along with increased spleen(P<0.01)and bursa of Fabricus weights(P<0.05),serum globulin contents,and the decreased intestine villus height and villus/crypt ratios(P<0.05).Notably,supplemental magnolol and honokiol attenuated these adverse changes,and the effects of magnolol were better than those of honokiol.Therefore,we performed RNA-Seq in ileum tissues and 16S rRNA gene sequencing of ileum bacteria.Our analysis revealed that magnolol increased the α-diversity(observed species,Chao1,ACE,and PD whole tree)and β-diversity of the ileum bacteria(P<0.05).In addition,magnolol supplementation increased the abundance of Lactobacillus(P<0.01)and decreased unidentified Cyanobacteria(P<0.05)both at d 14 and d 21.Further study confirmed that differentially expressed genes induced by magnolol and honokiol supplementation enriched in cytokine-cytokine receptor interactions,in the intestinal immune network for IgA production,and in the cell adhesion molecule pathways.Conclusions:Supplemental magnolol and honokiol alleviated S.pullorum-induced impairments in growth performance,and the effect of magnolol was better than that of honokiol,which could be partially due to magnolol’s ability to improve the intestinal microbial and mucosal barrier.
基金supported by grants from the National Key Research and Development Program of China (2016YFD0501608)the National Natural Science Foundation of China (31470893)+1 种基金the Special Fund for Agro-scientific Research in the Public Interest,China (201403054)the National High Technology Research and Development Program of China (2011AA10A210)
文摘In this study, a safety enhanced Salmonella Pullorum （S. Pullorum） ghost was constructed using an antimicrobial peptide gene, and evaluated for its potential as a Pullorum disease （PD） vaccine candidate. The antimicrobial peptide SMAP29 was co-expressed with lysis gene E to generate S. Pullorum ghosts. No viable bacteria were detectable either in the fermentation culture after induction of gene E- and SMAP29-mediated lysis for 24 h or in the lyophilized ghost products. Specific-pathogen- free （SPF） chicks were intraperitoneally immunized with ghosts at day 7 of age and no mortality, clinical symptoms or signs of PD such as anorexia, depression and diarrhea were observed. On challenge with a virulent S. Pullorum strain at 4 wk post-immunization, a comparatively higher level of protection was observed in the S. Pullorum ghost immunized chickens with a minimum of pathological lesions and bacterial loads compared to the birds in inactivated vaccine groups. In addition, immunization with the S. Pullorum ghosts induced a potent systemic IgG response and was associated with significantly increased levels of cytokine IFN-y and IL-4 and relative percentages of CD4＋ and CD8＋ T lymphocytes. Our results indicate that SMAP29 can be employed as a new secondary lethal protein to enhance the safety of bacterial ghosts, and to prepare a non-living bacterial vaccine candidate that can prevent PD in chickens.
文摘The isolation and culture of conventional detection method of salmonella can not meet the testing requirements of quick and easy detection at the grassroots level. In this study, we prepare the fluorescent nanoparticles as a marker, covalently conjugate with monoclonal antibodies of Salmonella pullorum. The whole Salmonella pullorum antigen and goat anti-mouse antibody sprayed on the nitrocellulose membranes are used as test line and control line. The fluorescence nanoparticles immune based lateral flow strips are made according to the principle of antigen-antibody immune response. The test strips may interpret results within 30 min. The results of the salmonella A, S. agona, S. chester and S. arechavaleta are positive, including, S. agona for weakly positive. After analysis, it is found that in addition to the salmonella of group A, the other positive salmonella are in group B. But it is negative of S. derby, S. rissen, and other 6 kinds of salmonella, with good specificity. The fluorescence nanoparticles immune based lateral flow strips are a little of sample can be detected fast, easily, inexpensive, easy to universal without professional technical personnel detection method. It provides a new detection method for the detections of Salmonella pullorum.
基金supported by the Earmarked Fund for the Modern Agroindustry Technology Research System, China (CARS-41)the National High Technology Research and Development Program of China (2011AA100301)
文摘supported by the Earmarked Fund for the Modern Agroindustry Technology Research System, China （CARS-41）;the National High Technology Research and Development Program of China （2011AA100301）
文摘BACKGROUND： For many years, yeast cell walls （YCW） and mannan oligosaccharides （MOS） have been used as alternatives to antibiotics and health feed additives to enhance the growth performance and health of food animals. In the present study, the inhibitory effects of YCW and MOS on the adhesion of enteropathogenic bacteria to intestinal epithelial cells were tested. METHODS： YCW and MOS were extracted from Saccharomyces cerevisiae （XM 0315）, and the morphology of YCW and MOS bound to pathogenic bacteria was observed by scanning electron microscopy （SEM）. Real-time fluorescent quantitative PCR was used to quantitatively analyze the effects of YCW and MOS on the adhesion of Escherichia coli （CVCC3367） and Salmonella pullorum （CVCC520） to Caco-2 cells. RESULTS： The results showed that YCW inhibited E. coli and S. pullorum binding to Caco-2 cells by 95% and 74%, respectively, whereas MOS prevented E. coli and S. pullorum binding by 67% and 50%, respectively. CONCLUSIONS： These data suggest that YCW has a stronger ability than MOS to inhibit pathogenic bacteria from adhering to Caco-2 cells in vitro.
