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Visual Analysis of Knowledge Map of Traditional Chinese Medicine in Prevention and Treatment of Pulmonary Interstitial Fibrosis Based on CiteSpace
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作者 张慧莹 蒋先伟 马战平 《World Journal of Integrated Traditional and Western Medicine》 2023年第2期24-36,共13页
Objective: To analyze the relevant research literature on the prevention and treatment of pulmonary interstitial fibrosis with traditional Chinese medicine (TCM), understand the current research status, hot spots and ... Objective: To analyze the relevant research literature on the prevention and treatment of pulmonary interstitial fibrosis with traditional Chinese medicine (TCM), understand the current research status, hot spots and future development trend in this field, and provide basis and feasible suggestions for further research in this field. Methods: The journal literatures related to the prevention and treatment of pulmonary interstitial fibrosis with TCM in recent 20 years in CNKI database were searched and passed through CiteSpace 5.8.R3 generates the knowledge map of relevant literature authors, document issuing institutions and keywords, and makes visual analysis. Results: A total of 1,576 documents were included, and the annual number of documents showed a fluctuating upward trend, forming a relatively stable research team represented by authors such as LYU Xiaodong, PANG Lijian and LIU Chuang;According to the atlas of document issuing institutions, Shandong University of Traditional Chinese Medicine and its affiliated hospitals ranked first in the number of documents issued, and the cooperation between institutions is dominated by the University of traditional Chinese medicine and its affiliated hospitals;Keyword cluster analysis shows that a large number of studies have been carried out in the field of etiology and pathogenesis, TCM compound, clinic and experiment. Conclusion: The research on the prevention and treatment of pulmonary interstitial fibrosis with TCM has a high degree of attention, but the cooperation network between the research authors and institutions needs to be strengthened. The research on the pathogenesis and improving the quality of life of patients is the trend of development in the future. 展开更多
关键词 pulmonary interstitial fibrosis CiteSpace software Knowledge atlas Visual analysis Traditional Chinese medicine
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Research progress of Chinese medicine in treatment of IPF(idiopathic pulmonary fibrosis)
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作者 Luo Haili Cai Lingling +5 位作者 Sun Song Cao Ting WU Meichao Wu Zhisong Fan Maorong Wang Shuchen 《World Journal of Integrated Traditional and Western Medicine》 2017年第2期18-21,共4页
Idiopathic pulmonary interstitial fibrosis is one of the respiratory refractory diseases,and the incidence rate is on the rise.At present,the effect of western medicine is not ideal and the side effects are obvious,wh... Idiopathic pulmonary interstitial fibrosis is one of the respiratory refractory diseases,and the incidence rate is on the rise.At present,the effect of western medicine is not ideal and the side effects are obvious,while the traditional Chinese medicine shows good curative effect on the disease.This paper makes a summary on the traditional Chinese medicine theory in treating idiopathic pulmonary interstitial fibrosis in recent years. 展开更多
关键词 Idiopathic pulmonary interstitial fibrosis Chinese medicine Research progress
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Interstitial lung disease and diabetes 被引量:10
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作者 Venkat Rajasurya Kulothungan Gunasekaran Salim Surani 《World Journal of Diabetes》 SCIE CAS 2020年第8期351-357,共7页
Diabetes mellitus (DM) is a chronic metabolic disease and its prevalence has beensteadily increasing all over the world. DM and its associated micro andmacrovascular complications result in significant morbidity and m... Diabetes mellitus (DM) is a chronic metabolic disease and its prevalence has beensteadily increasing all over the world. DM and its associated micro andmacrovascular complications result in significant morbidity and mortality. Themicrovascular complications are usually manifested as retinopathy, neuropathy,nephropathy and macrovascular complications generally affect the cardiovascularsystem. In addition to these complications, DM also affects the lungs because of itsrich vascularity and abundance in connective tissue (collagen and elastin). DMhas been found to cause microvascular complications and proliferation ofextracellular connective tissue in the lungs, leading to decline in lung function in arestrictive pattern. Interstitial lung disease (ILD) includes a diverse group ofdisease conditions characterized by different degrees of inflammation and fibrosisin the pulmonary parenchyma. Idiopathic pulmonary fibrosis (IPF) is one of thecommon type of idiopathic interstitial pneumonia with a high mortality rate. IPFis characterized by chronic progressive fibrosis leading to progressive respiratoryfailure. In this review we focus on lung as the target organ in DM and theassociation of DM and ILD with special emphasis on IPF. 展开更多
关键词 Diabetes mellitus interstitial lung disease Idiopathic pulmonary fibrosis METFORMIN Diabetes complications pulmonary function test Restrictive lung disease HYPERGLYCEMIA
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Sleep disordered breathing in interstitial lung disease: A review 被引量:2
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作者 Lauren K Troy Tamera J Corte 《World Journal of Clinical Cases》 SCIE 2014年第12期828-834,共7页
Patients with interstitial lung disease commonly exhibit abnormal sleep architecture and increased sleep fragmentation on polysomnography. Fatigue is a frequent complaint, and it is likely that poor sleep quality is a... Patients with interstitial lung disease commonly exhibit abnormal sleep architecture and increased sleep fragmentation on polysomnography. Fatigue is a frequent complaint, and it is likely that poor sleep quality is a significant contributor. A number of studies have shown that sleep disordered breathing is prevalent in this population, particularly in the idiopathic pulmonary fibrosis subgroup. The factors that predispose these patients to obstructive sleep apnoea are not well understood, however it is believed that reduced caudal traction on the upper airway can enhance collapsibility. Ventilatory control system instability may also be an important factor, particularly in those with increased chemo-responsiveness, and in hypoxic conditions. Transient, repetitive nocturnal oxygen desaturation is frequently observed in interstitial lung disease, both with and without associated obstructive apnoeas. There is increasing evidence that sleep-desaturation is associated with increased mortality, and may be important in the pathogenesis of pulmonary hypertension in this population. 展开更多
关键词 SLEEP DISORDERED breathing interstitial lung disease pulmonary fibrosis NOCTURNAL hypoxia OBSTRUCTIVE SLEEP APNOEA
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Polymyxin B-immobilized fiber columns:A column to breathe new life into the treatment of interstitial lung disease? 被引量:2
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作者 Katsuaki Asakawa Toshinori Takada 《World Journal of Respirology》 2015年第1期1-3,共3页
Acute exacerbations of idiopathic pulmonary fibrosis(IPF) is a severe respiratory condition with high mortality rate. Direct hemoperfusion with polymyxin B-immobilized fiber columns(PMX-DHP) was originally introduced ... Acute exacerbations of idiopathic pulmonary fibrosis(IPF) is a severe respiratory condition with high mortality rate. Direct hemoperfusion with polymyxin B-immobilized fiber columns(PMX-DHP) was originally introduced for the treatment of septic shock. Application of PMX-DHP to the treatment of acute exacerbations of IPF may improve oxygenation and survival of the patients with the disease. In addition to acute exacerbations of IPF, PMXDHP has been applied to acute respiratory failure fromvarious causes; an amyopathic dermatomyositis patient who developed rapidly progressive interstitial lung disease(ILD) with elevated anti-CADM-140/MDA5 autoantibody and a patient with severe amiodarone pulmonary toxicity. It is also demonstrated that PMX-DHP performed on the first day of steroid pulse therapy may improve the prognosis of patients with rapidly progressive ILDs in a case-control setting. PMX treatment decreases not only various circulating molecules but also inflammatory cells, in particular activated monocytes, producing such mediators. Although the incidence of acute exacerbations of IPF is too low for proper randomization, in order to test the effects of PMX-DHP on the disease, a cohort or casecontrol analytic study needs to be conducted, preferably from more than one center or research group. 展开更多
关键词 Acute EXACERBATION IDIOPATHIC pulmonary fibrosis POLYMYXIN B HEMOPERFUSION interstitial lung disease
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Acute exacerbation of interstitial lung disease in the intensive care unit:Principles of diagnostic evaluation and management
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作者 Muhammad K Hayat Syed Or Bruck +1 位作者 Anupam Kumar Salim Surani 《World Journal of Critical Care Medicine》 2023年第3期153-164,共12页
Interstitial lung disease(ILD)is typically managed on an outpatient basis.Critical care physicians manage patients with ILD in the setting of an acute exacerbation(ILD flare)causing severe hypoxia.The principles of ma... Interstitial lung disease(ILD)is typically managed on an outpatient basis.Critical care physicians manage patients with ILD in the setting of an acute exacerbation(ILD flare)causing severe hypoxia.The principles of management of acute exacerbation of ILD are different from those used to manage patients with acute respiratory distress syndrome from sepsis,etc.Selected patients may be candidates for aggressive measures like extracorporeal membrane oxygenation and lung transplantation,while almost all patients will benefit from early palliative care.This review focused on the types of ILD,diagnosis,and management pathways for this challenging condition. 展开更多
关键词 interstitial lung disease pulmonary fibrosis Acute exacerbation of interstitial lung disease Extracorporeal membrane oxygenation interstitial lung disease flare IMMUNOSUPPRESSION
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Acute exacerbation of idiopathic pulmonary fibrosis: usual interstitial pneumonitis vs.possible usual interstitial pneumonitis pattern
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作者 Meng-Shu Cao Jian Sheng +5 位作者 Tian-Zhen Wang Xiao-Hua Qiu Dong-Mei Wang Yang Wang Yong-Long Xiao Hou-Rong Cai 《Chinese Medical Journal》 SCIE CAS CSCD 2019年第18期2177-2184,共8页
Background:The prognosis of acute exacerbation of idiopathic pulmonary fibrosis (AE-IPF) is very poor with a high mortality.The aim of this study was to describe the clinical features and survival of patients with AE-... Background:The prognosis of acute exacerbation of idiopathic pulmonary fibrosis (AE-IPF) is very poor with a high mortality.The aim of this study was to describe the clinical features and survival of patients with AE-IPF with usual pulmonary fibrosis (UIP) and possible UIP (P-UIP) pattern on chest high resolution computed tomography (HRCT).Methods:This retrospective study included 107 patients with AE-IPF admitted to Nanjing Drum Tower Hospital from January 2010 to December 2016.The subjects were divided into UIP (n =86) and P-UIP group (n=21) based on chest HRCT.Continuous variables were analyzed using Student's t test or Mann-Whimey U test.Categorical variables were analyzed using x2 test.Log-rank test was used for the survival analysis.Cox proportional models evaluated the risk factors for AE occurrence and survival.Results:The male,older patients,previous N-acetylcysteine use,elevated white blood cell (WBC) counts,and microbiology infection were more common in the UIP group than the P-UIP group (X2 =13.567,P < 0.001;z =-2.936,P =0.003;X2 =5.901,P =0.015;t =2.048,P =0.043;x2 =10.297,P =0.036,respectively).The percentage of AE with UIP pattern in idiopathic interstitial pneumonia (ⅡP) was significantly higher than P-UIP pattern (X2 =40.011,P < 0.001).Smoking was the risk factor for AE within 6 months after IPF diagnosis in the UIP group.The cumulative proportion survival of 30-days was significantly higher in the UIP group compared with the P-UIP group (x2 =5.489,P =0.019) despite of the similar overall survival in the two groups.Multivariate Cox regression analysis indicated WBC count,partial pressure of oxygen in artery (PaO2)/ffactional concentration of inspired oxygen (FiOz),and computed tomography (CT) score were the independent predictors for survival in the UIP group (hazard ratio [HR]:1.070,95% confidential interval [CI]:1.027-1.114,P=0.001;HR:0.992,95% CI:0.986-0.997,P=0.002;and HR:1.649,95% CI:1.253-2.171,P < 0.001,respectively).Conclusions:AE occurrence of UIP patients in IIP was significantly more than P-UIP cases.The short-term survival was better in the UIP group despite of the similar overall survival in the two groups.WBC count,PaO2/FiO2,and CT score were the independent predictors for survival in UIP subjects. 展开更多
关键词 IDIOPATHIC pulmonary fibrosis Acute EXACERBATION Usual interstitial PNEUMONITIS
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补肾益肺消癥方治疗老年COPD合并PIF的疗效及对患者血清VEGF、PAI-1、VCAM-1和肺功能的影响 被引量:4
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作者 苏垠旭 吴丹 +1 位作者 李婧 曲萌 《海南医学》 CAS 2023年第10期1388-1392,共5页
目的 观察补肾益肺消癥方治疗老年慢性阻塞性肺疾病(COPD)合并肺间质纤维化(PIF)的疗效,并探讨其对患者血清中血管内皮生长因子(VEGF)、纤溶酶原激活物抑制物1 (PAI-1)、血管细胞黏附因子(VCAM-1)和肺功能的影响。方法 收集2019年4月至2... 目的 观察补肾益肺消癥方治疗老年慢性阻塞性肺疾病(COPD)合并肺间质纤维化(PIF)的疗效,并探讨其对患者血清中血管内皮生长因子(VEGF)、纤溶酶原激活物抑制物1 (PAI-1)、血管细胞黏附因子(VCAM-1)和肺功能的影响。方法 收集2019年4月至2022年4月就诊于宝鸡市人民医院的102例老年COPD合并PIF患者为研究对象,采用随机数表法将患者分为观察组和对照组各51例,对照组患者以西药方式进行对症治疗,观察组患者在对照组基础上加用补肾益肺消癥方,疗程为2个月。比较两组患者的临床治疗效果,治疗前后的中医症状积分、血清VEGF、PAI-1、VCAM-1水平及肺功能指标[用力肺活量(FVC)、一秒用力呼气容积(FEV1)、FEV1/FVC]变化。结果 观察组患者的治疗总有效率为90.20%,明显高于对照组的72.55%,差异具有统计学意义(P<0.05);治疗前,两组患者的中医症状积分比较差异无统计学意义(P>0.05),治疗后,观察组患者的喘促、咳嗽、胸闷积分分别为(1.77±0.51)分、(1.88±0.59)分、(1.57±0.45)分,明显低于对照组的(2.67±0.83)分、(2.55±0.76)分、(2.17±0.63)分,差异均具有统计学意义(P<0.05);治疗后,两组患者的血清VEGF、PAI-1、VCAM-1水平均较治疗前明显降低,且观察组分别为(160.97±20.17) ng/L、(49.98±12.58) pg/mL、(359.17±103.05)μg/L,明显低于对照组的(171.17±21.53) ng/L、(57.65±13.36) pg/mL、(413.17±124.13)μg/L,差异均有统计学意义(P<0.05);治疗前,两组患者的肺通气功能比较差异无统计学意义(P>0.05),治疗后,观察组患者的FEV1、FVC、FEV1/FVC%分别为(3.07±0.87) L、(3.58±1.09) L、(80.19±8.05)%,明显高于对照组的(1.97±0.43) L、(2.95±0.86) L、(66.26±7.19)%,差异均具有统计学意义(P<0.05)。结论 补肾益肺消癥方对老年COPD合并PIF的治疗效果较好,且能够降低患者的血清VEGF、PAI-1、VCAM-1水平,改善肺脏通气功能。 展开更多
关键词 慢性阻塞性肺疾病 肺间质纤维化 补肾益肺消癥方 肺功能 疗效
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Promising new treatment targets in patients with fibrosing lung disorders
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作者 Martina Sterclova Martina Vasakova 《World Journal of Clinical Cases》 SCIE 2014年第11期668-675,共8页
The processes of lung fibrogenesis and fibrotic healing are common to a number of conditions with different etiologies. The lungs are the only affected organ in some cases, whereas in others, several organ systems are... The processes of lung fibrogenesis and fibrotic healing are common to a number of conditions with different etiologies. The lungs are the only affected organ in some cases, whereas in others, several organ systems are involved. Therapeutic options can be discussed from various perspectives. In this review, we address the localization of therapeutic targets with regard to cell compartments, including secreted ligands, cell surface, plasma membrane-cytosol interplay, cytosol and nucleus. Complex approach using stem cell therapy is also discussed. As the prognosis of patients with these disorders remains grim, treatment combinations targeting different molecules within the cell should sometimes be considered. It is reasonable to assume that blocking specific pathways will more likely lead to disease stabilization, while stem cell-based treatments could potentially restore lung architecture. Gene therapy could be a candidate for preventive care in families with proven specific gene polymorphisms and documented familial lung fibrosis. Chronobiology, that takes into account effect of circadian rhythm on cell biology, has demonstrated that timed drug administration can improve treatment outcomes. However, the specificrecommendations for optimal approaches are still under debate. A multifaceted approach to interstitial lung disorders, including cooperation between those doing basic research and clinical doctors as well as tailoring research and treatment strategies toward(until now) unmet medical needs, could improve our understanding of the diseases and, above all, provide benefits for our patients. 展开更多
关键词 interstitial LUNG DISEASE TREATMENT IDIOPATHIC pulmonary fibrosis CONNECTIVE tissue DISEASE Cell COMPARTMENTS Signaling molecules Signal transducers Transcription factors
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白三烯受体拮抗剂治疗PIF-COPD的临床效果 被引量:2
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作者 冀新红 《临床医学研究与实践》 2019年第9期30-31,共2页
目的评价白三烯受体拮抗剂治疗慢性阻塞性肺疾病继发肺间质纤维化(PIF-COPD)的临床效果。方法选择收治的96例PIF-COPD患者为研究对象,随机将其分为对照组(n=48)与观察组(n=48)。对照组患者给予祛痰、支气管扩张剂、肺功能锻炼等常规治疗... 目的评价白三烯受体拮抗剂治疗慢性阻塞性肺疾病继发肺间质纤维化(PIF-COPD)的临床效果。方法选择收治的96例PIF-COPD患者为研究对象,随机将其分为对照组(n=48)与观察组(n=48)。对照组患者给予祛痰、支气管扩张剂、肺功能锻炼等常规治疗,观察组在常规治疗基础上行白三稀受体拮抗剂治疗。比较两组患者治疗效果。结果治疗后,两组MMRC评分均降低,FVC%、FEV1%及6MWT均显著升高,且观察组优于对照组(P<0.05)。结论白三烯受体拮抗剂治疗PIF-COPD效果显著,值得推广。 展开更多
关键词 白三烯受体拮抗剂 慢性阻塞性肺疾病 肺间质纤维化 肺功能
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The Expression of Matrix Metalloproteinases and Tissue Inhibitors of Metalloproteinases in Idiopathic Interstitisal Pneumonia
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作者 Ji Young Shin Yu Jin Kim +2 位作者 Sun Young Kyung Seung Yeon Ha Sung Hwan Jeong 《Open Journal of Respiratory Diseases》 2014年第3期101-109,共9页
Background: Idiopathic interstitial pneumonia is characterized by fibroblast proliferation and extracellular matrix (ECM) accumulation. Matrix metalloproteases (MMPs) and tissue inhibitors of metalloproteases (TIMPs) ... Background: Idiopathic interstitial pneumonia is characterized by fibroblast proliferation and extracellular matrix (ECM) accumulation. Matrix metalloproteases (MMPs) and tissue inhibitors of metalloproteases (TIMPs) have been shown to regulate remodeling of the ECM, which indicates that they are important factors in the process of lung fibrosis. Therefore, we evaluated the expression of MMPs and TIMPs in tissues obtained from patients with idiopathic interstitial pneumonia and control tissues. Methods: Thirty-seven patients who were diagnosed with IIP (22: IPF, 13: NSIP, 2: COP) and 5 controls were enrolled in this study. The MMP-2 and -9 activity in lung tissue obtained from these patients was analyzed using gelatin zymography and the levels of TIMP-1 and -2 were measured by western blotting. We also evaluated the expression of MMP-2 and -9, as well as that of TIMP-1 and -2 in lung tissue using immunohistochemistry. Results: The levels of MMP-2 and MMP-9 were significantly increased in patients with IPF compared to those with NSIP and COP. The activities of TIMP-1 and -2 were also higher in patients with IPF than NSIP/COP patients and control subjects. There were no significant differences observed in the activities of MMPs and TIMPs obtained from patients with NSIP/COP and control subjects. The immunohistochemical analysis showed that TIMP-2 and MMP-2 were strongly stained at the fibroblasts of the fibroblastic foci in patients with IPF. Conclusions: These results suggest that over-expression of gelatinases and TIMPs in patients with IPF are important factors in the irreversible fibrosis that is associated with lung parenchyma. 展开更多
关键词 IDIOPATHIC interstitial PNEUMONIA IDIOPATHIC pulmonary fibrosis Matrix METALLOPROTEINASES Tissue Inhibitor of Matalloproteinases NONSPECIFIC interstitial PNEUMONIA CRYPTOGENIC Organizing PNEUMONIA
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CTD-ILD合并肺部感染患者肺部微生物菌群特点及对肺纤维化的影响
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作者 王小丽 张旭静 李英 《河南医学研究》 CAS 2024年第6期997-1001,共5页
目的探讨结缔组织病相关间质性肺疾病(CTD-ILD)合并肺部感染患者肺部微生物菌群特点及对肺纤维化的影响。方法选取2021年2月至2022年5月在医院治疗的45例CTD-ILD合并肺部感染患者作为观察组,同时选取45例CTD-ILD未合并肺部感染患者作为... 目的探讨结缔组织病相关间质性肺疾病(CTD-ILD)合并肺部感染患者肺部微生物菌群特点及对肺纤维化的影响。方法选取2021年2月至2022年5月在医院治疗的45例CTD-ILD合并肺部感染患者作为观察组,同时选取45例CTD-ILD未合并肺部感染患者作为对照组,比较两组肺功能、高分辨CT(HRCT)肺纤维化评分,同时分析观察组病原菌分布情况。结果观察组用力肺活量占预计值百分比(FVC%pred)、第1秒用力呼气容积占预计值百分比(FEV 1%pred)、一氧化碳弥散量占预计值百分比(DLCO%pred)、用力肺活量(FVC)、最大呼气流量(MEF)和残气容积(RV)低于对照组(P<0.05);观察组HRCT影像学斑片状、支气管扩张比率高于对照组(P<0.05),CT纤维化评分高于对照组(P<0.05)。观察组病原菌分布:细菌分布中以肺炎克雷伯菌、铜绿假单胞菌为主,分别占24.44%和15.56%,病毒检出巨细胞病毒、人类疱疹病毒,分别占11.11%和6.67%,真菌检出白念珠菌,占11.11%。观察组年龄≥60岁患者CT纤维化评分高于年龄<60岁患者(P<0.05)。结论CTD-ILD合并肺部感染患者以细菌感染为主;相比较未合并肺部感染患者,合并肺部感染患者肺纤维化较重。 展开更多
关键词 结缔组织病相关间质性肺疾病 病原菌 肺纤维化 肺部感染
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保真汤治疗气阴两虚型肺间质纤维化的临床效果
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作者 庞彩苓 王培 王萍 《反射疗法与康复医学》 2024年第1期39-41,45,共4页
目的分析气阴两虚型肺间质纤维化患者采用保真汤治疗的效果.方法选取2018年9月—2020年12月潍坊市中医院收治的80例气阴两虚型肺间质纤维化患者为研究对象,以随机抽签法将其分为对照组及观察组,每组40例.对照组采用常规西药治疗,观察组... 目的分析气阴两虚型肺间质纤维化患者采用保真汤治疗的效果.方法选取2018年9月—2020年12月潍坊市中医院收治的80例气阴两虚型肺间质纤维化患者为研究对象,以随机抽签法将其分为对照组及观察组,每组40例.对照组采用常规西药治疗,观察组采用保真汤治疗.比较两组的治疗效果.结果观察组的治疗总有效率为95.00%,高于对照组的77.50%,差异有统计学意义(P﹤0.05).治疗后,观察组的用力肺活量(FVC)、第1秒用力呼气容积(FEV_(1))及FEV_(1)/FVC水平均高于对照组,组间差异有统计学意义(P﹤0.05);观察组的各项中医证候积分均低于对照组,组间差异有统计学意义(P﹤0.05);观察组的生活质量综合评定问卷中各项评分均高于对照组,组间差异有统计学意义(P﹤0.05).结论气阴两虚型肺间质纤维化患者采用保真汤治疗的效果显著,可改善其肺功能,减轻症状,提高生活质量,值得临床推广使用. 展开更多
关键词 肺间质纤维化 气阴两虚型 保真汤 治疗效果
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基于网络药理学探讨白芍总苷治疗类风湿关节炎肺间质纤维化的机制研究 被引量:1
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作者 邢清桦 李松伟 +2 位作者 龚晓红 胡文盈 陆超群 《中国畜牧兽医》 CAS CSCD 北大核心 2024年第2期850-863,共14页
[目的]通过网络药理学结合动物试验探究白芍总苷(TGP)主要药物成分与类风湿关节炎(RA)肺间质纤维化之间的作用靶点,揭示白芍总苷治疗RA肺间质纤维化的分子机制。[方法]通过检索多个数据库,根据2个ADEM参数口服生物利用度(oral bioavaila... [目的]通过网络药理学结合动物试验探究白芍总苷(TGP)主要药物成分与类风湿关节炎(RA)肺间质纤维化之间的作用靶点,揭示白芍总苷治疗RA肺间质纤维化的分子机制。[方法]通过检索多个数据库,根据2个ADEM参数口服生物利用度(oral bioavailability, OB)>30%、类药性(drug likeness, DL)>0.18,筛选出白芍总苷中的主要活性成分及作用的基因靶点;检索DrugBank、GeneCards、OMIM、TTD、DisGenet等数据库筛选RA和肺间质纤维化的基因靶点,通过UniProt数据库进行靶点蛋白的基因名转换,收集白芍总苷治疗RA和肺间质纤维化的作用靶点。获取化合物和疾病靶点取交集,制作韦恩图;将靶点交集导入到STRING数据库绘制PPI网络图,利用DAVID数据库进行GO功能和KEGG信号通路富集分析,深入分析其治疗类风湿关节炎合并肺间质纤维化的机制。将Wistar大鼠分为空白组、模型组、白芍总苷组和托法替布组,通过测定肺脏指数反映肺脏组织的水肿炎症反应,HE染色观察各组肺组织和踝关节滑膜租住的炎症反应,Masson染色观察肺脏组织的胶原沉积状况。[结果]检索TCMSP数据库,筛选得到85种白芍总苷的药物成分,根据OB>30%、类药性>0.18,共筛选9种主要成分;从DrugBank、DisGenet、OMIM、TTD和GeneCards等数据库筛选RA疾病靶点1 625个,肺间质纤维化的疾病靶点1 930个。通过GO功能和KEGG信号通路富集分析共获得分子功能65条目,细胞组分37条目,生物过程350条目和141个富集通路。通过中药-靶点-通路网络图,将Degree排名前十的肿瘤坏死因子(TNF)、白细胞介素-6(IL6)、AKT丝氨酸/苏氨酸激酶1(AKT1)、血管内皮生长因子A(VEGFA)、基质金属蛋白酶9 (MMP9)、转录因子Jun(JUN)、过氧化物酶体增殖物激活受体γ(PPARG)、胱天蛋白酶3(CASP3)、前列腺素内过氧化物合酶2(PTGS2)、细胞间黏附分子-1(ICAM1)作为白芍总苷治疗RA肺间质纤维化的关键作用靶点。动物试验结果显示,与模型组相比,治疗组的关节炎评分和肺脏指数降低;HE染色发现,白芍总苷组的关节和肺脏组织炎性细胞的浸润减少;Masson染色发现,白芍总苷可减少条索状的纤维化灶,表明白芍总苷不但可治疗RA,还能延缓肺间质纤维化的胶原沉积和减少炎症反应。[结论]白芍总苷通过多种成分作用于炎症反应、免疫调节和细胞分化增殖等方面的多靶点和多通路,通过靶点和通路间的协同相互作用来延缓RA肺间质纤维化疾病的发展进程。 展开更多
关键词 网络药理学 白芍总苷 类风湿关节炎 肺间质纤维化 作用机制
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雷公藤甲素通过抑制细胞铁死亡减轻博来霉素诱发的小鼠肺纤维化
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作者 张致琴 吴洁 +6 位作者 秦艺璐 梁舒 左淑飞 张超 高晓 范文强 张志强 《中国病理生理杂志》 CAS CSCD 北大核心 2024年第4期662-669,共8页
目的:探讨雷公藤甲素(TPL)对博来霉素(BLM)诱导肺纤维化小鼠的改善效果,揭示铁死亡参与TPL改善肺纤维化的潜在机制。方法:选择SPF级小鼠,随机分为3组:对照组、模型组(BLM处理组)和实验组(BLM+TPL处理组),每组10只。Day 0,模型组和实验... 目的:探讨雷公藤甲素(TPL)对博来霉素(BLM)诱导肺纤维化小鼠的改善效果,揭示铁死亡参与TPL改善肺纤维化的潜在机制。方法:选择SPF级小鼠,随机分为3组:对照组、模型组(BLM处理组)和实验组(BLM+TPL处理组),每组10只。Day 0,模型组和实验组小鼠气管灌注BLM水溶液(50μL,5 mg/kg),对照组小鼠接受等体积的生理盐水。Day 1起,实验组小鼠开始灌胃TPL悬液(200μL,0.25 mg/kg),每3 d一次,共7次,对照组和模型组分别接受等体积生理盐水。Masson染色检测肺组织纤维化,TUNEL染色检测肺组织细胞凋亡;体外CCK8法检测细胞活力,流式细胞术及活死染色检测细胞凋亡,免疫荧光法检测细胞脂质过氧化,Western blot检测肺组织及细胞中目的蛋白表达。结果:TPL处理通过下调肺组织中Ⅰ型胶原(Col Ⅰ)和α-平滑肌肌动蛋白(α-SMA)的表达(P<0.05),显著降低BLM诱导小鼠肺部纤维化程度(P<0.01);同时,TPL处理可显著上调肺组织中谷胱甘肽过氧化酶4(GPX4)和铁死亡抑制蛋白1(FSP1)表达(P<0.05),下调转铁蛋白受体1(TfR1)表达(P<0.05),从而抑制BLM诱导的肺部细胞铁死亡和凋亡(P<0.01)。体外研究结果表明,TPL处理可显著抑制肺上皮细胞凋亡(P<0.01),进一步检测发现TPL处理可显著降低BLM诱导的肺泡上皮细胞内脂质过氧化(P<0.01),显著上调细胞内GPX4和FSP1蛋白表达(P<0.01),下调TfR1蛋白表达(P<0.05),通过抑制细胞铁死亡来显著降低BLM诱导的肺上皮细胞凋亡率。结论:TPL可在体内外通过抑制BLM诱导的细胞铁死亡,改善肺部细胞活力,降低细胞凋亡率,从而减轻肺小鼠纤维化程度。本研究为临床使用TPL治疗肺间质病变提供理论依据。 展开更多
关键词 雷公藤甲素 肺纤维化 肺间质病变 铁死亡 博来霉素
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人工智能在特发性肺纤维化中的研究进展 被引量:1
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作者 王艳艳 吴华伟(审校) 《国际医学放射学杂志》 2024年第1期48-52,共5页
特发性肺纤维化(IPF)是一种可致死的慢性进行性纤维化间质性肺炎。早期诊断并及时开始抗纤维化治疗是延长IPF病人生存时间的关键。人工智能(AI)技术能够从影像数据中自动学习人眼无法识别的特征,在计算机辅助检测(CAD)系统中的应用可以... 特发性肺纤维化(IPF)是一种可致死的慢性进行性纤维化间质性肺炎。早期诊断并及时开始抗纤维化治疗是延长IPF病人生存时间的关键。人工智能(AI)技术能够从影像数据中自动学习人眼无法识别的特征,在计算机辅助检测(CAD)系统中的应用可以提供决策支持,有助于提高IPF的诊断准确性并预测疾病进展。就AI的相关概念及其在IPF诊断、疾病进展与预后预测以及药物治疗反应评估中的研究现状予以综述。 展开更多
关键词 特发性肺纤维化 间质性肺炎 人工智能 计算机辅助检测 体层摄影术 X线计算机
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LncRNA FEZF1-AS1通过调控EZH2对肺间质细胞增殖、迁移及侵袭的作用 被引量:1
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作者 王春燕 王萍 +2 位作者 宋龙飞 刘永全 满君 《基础医学与临床》 2024年第1期43-50,共8页
目的研究长链非编码RNA FEZ家族锌指1-反义RNA 1(lncRNA FEZF1-AS1)调控zeste同源物增强子2(EZH2)对肺间质细胞增殖、迁移、侵袭能力及上皮细胞-间质转化(EMT)的影响及其作用机制。方法将人肺腺癌细胞系A549分为对照组(control)和模型组... 目的研究长链非编码RNA FEZ家族锌指1-反义RNA 1(lncRNA FEZF1-AS1)调控zeste同源物增强子2(EZH2)对肺间质细胞增殖、迁移、侵袭能力及上皮细胞-间质转化(EMT)的影响及其作用机制。方法将人肺腺癌细胞系A549分为对照组(control)和模型组[model,用转化生长因子β1(TGF-β1)20 ng/mL作用48 h,诱导成为肺间质细胞]。用Western blot检测细胞中E-钙黏蛋白(E-cadherin)、N-钙黏蛋白(N-cadherin)及波形蛋白(vimentin)的蛋白表达。RT-qPCR检测细胞中lncRNA FEZF1-AS1和EZH2基因表达。转染组细胞分为转染si NC组、si lncRNA FEZF1-AS1+OE vector组和si lncRNA FEZF1-AS1+OE EZH2组。CCK-8法检测细胞增殖、细胞划痕检测细胞迁移、Transwell小室法检测细胞侵袭;用Western blot检测细胞中E-cadherin、N-cadherin、vimentin及EZH2的蛋白表达,用RNA免疫沉淀(RIP)测定FEZF1-AS1与EZH2的直接结合作用。结果与对照组比较,模型组E-cadherin的蛋白表达水平减少(P<0.05);N-cadherin及vimentin的蛋白表达水平升高(P<0.05);与对照组比较,模型组lncRNA FEZF1-AS1与EZH2基因的表达水平明显升高(P<0.05);与si NC组相比,si lncRNA FEZF1-AS1+OE vector组细胞增殖、迁移、侵袭能力降低,E-cadherin蛋白表达升高,N-cadherin、vimentin、EZH2蛋白表达降低(P<0.05);与si lncRNA FEZF1-AS1+OE vector组比较,si lncRNA FEZF1-AS1+OE EZHZ组细胞增殖、侵袭、迁移能力升高,E-cadherin蛋白表达降低,N-cadherin、vimentin、EZH2蛋白表达升高(P<0.05);RIP实验进一步证实了lncRNA FEZF1-AS1与EZH2具有结合作用。结论LncRNA FEZF1-AS1通过调控EZH2促进肺间质细胞增殖、侵袭、转移和EMT过程。 展开更多
关键词 特发性肺间质纤维化 FEZ家族锌指1-反义RNA 1(FEZF1-AS1) 上皮细胞-间充质转化(EMT) zeste基因增强子同源物2(EZH2) 人非小细胞肺癌细胞系A549
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系统性硬化患者间质性肺病发生及进展的影响因素分析
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作者 刘娟 张瑾 +4 位作者 俞秋霞 刘冰冰 刘伟丽 王晓东 丁健 《浙江医学》 CAS 2024年第17期1830-1834,1839,共6页
目的探讨系统性硬化(SSc)发生间质性肺病(ILD)以及出现进展性肺纤维化(PPF)的临床特点和影响因素。方法回顾性收集2017年1月至2021年12月宁波市医疗中心李惠利医院风湿科门诊收治的75例SSc患者为研究对象。根据是否合并ILD,将患者分为SS... 目的探讨系统性硬化(SSc)发生间质性肺病(ILD)以及出现进展性肺纤维化(PPF)的临床特点和影响因素。方法回顾性收集2017年1月至2021年12月宁波市医疗中心李惠利医院风湿科门诊收治的75例SSc患者为研究对象。根据是否合并ILD,将患者分为SSc合并ILD(SSc-ILD)组41例和SSc未合并ILD(SSc-nILD)组34例。采用单因素和多因素logistic回归分析SSc患者发生ILD的影响因素。采用单因素logistic回归分析SSc-ILD进展为PPF的影响因素。以肺部高分辨率CT诊断ILD为观察起点,出现PPF为观察终点,绘制Kaplan-Meier生存曲线,采用log-rank检验比较抗Scl-70抗体(ATA)阳性组和ATA阴性组患者发生PPF的中位时间。结果SSc-ILD组患者病程长于SSc-nILD组,弥漫型SSc比例、指/趾端溃疡和(或)凹陷性瘢痕、胃食管反流、肺动脉高压发生率、修订Rodnan评分、ATA阳性率均高于SSc-nILD组,抗着丝点抗体(ACA)阳性率低于SScnILD组,差异均有统计学意义(均P<0.05)。多因素logistic回归分析发现,病程长是SSc患者发生ILD的独立危险因素(OR=1.017,95%CI:1.005~1.029,P=0.005),而ACA是SSc患者发生ILD的独立保护因素(OR=0.102,95%CI:0.017~0.621,P=0.013)。41例SSc-ILD患者中,进展为PPF(PPF组)11例,未进展为PPF(nPPF组)30例。PPF组和nPPF组患者ATA阳性率比较,差异有统计学意义(P<0.05)。单因素logistic回归分析发现ATA阳性是SSc-ILD进展为PPF的危险因素(OR=5.330,95%CI:1.156~24.598,P=0.032)。将PPF患者进一步分为ATA阳性组8例和ATA阴性组3例,进行生存分析,结果显示ATA阳性组患者发生PPF的中位时间为12.0(95%CI:6.8~17.2)个月,明显短于ATA阴性组的48.0(95%CI:37.5~58.5)个月,两组比较差异有统计学意义(P=0.033)。结论长病程可以作为SSc发生ILD的影响因素,而ATA阳性可以作为SSc-ILD患者出现PPF的影响因素。 展开更多
关键词 系统性硬化 间质性肺病 进展性肺纤维化 临床特点 影响因素
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进展性肺纤维化表现的结缔组织病相关间质性肺疾病患者临床管理(四川省)专家共识
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作者 四川省医疗卫生与健康促进会呼吸与危重症医学专业委员会间质性肺疾病多学科诊治学组 郭璐 +1 位作者 孙伟 刘倩茜 《实用医院临床杂志》 2024年第2期29-38,共10页
结缔组织病相关间质性肺疾病(connective tissue diseases associated with interstitial lung disease,CTD-ILD)很容易出现进展性肺纤维化(progressive pulmonary fibrosis,PPF)表现而预后不良。然而,如何管理PPF表现的CTD-ILD患者的... 结缔组织病相关间质性肺疾病(connective tissue diseases associated with interstitial lung disease,CTD-ILD)很容易出现进展性肺纤维化(progressive pulmonary fibrosis,PPF)表现而预后不良。然而,如何管理PPF表现的CTD-ILD患者的临床认识仍然非常有限;如何规范化治疗这部分患者也是经常面临的临床问题。为此,四川省医疗卫生与健康促进会呼吸与危重症医学专业委员会间质性肺疾病学组邀请了省内ILD相关领域专家组成了共识编写组,根据临床收集问题,组织专家讨论,最终确定纳入了5个方面的内容。相关内容主要包括CTD-ILD出现PPF的高危因素、临床表现与诊断、病情评估、随访管理和治疗等方面。基于国内外指南、临床研究数据等循证证据,经过多次讨论和投票表决,形成9条推荐意见,共同制定了《PPF表现的CTD-ILD患者临床管理(四川省内)专家共识》,旨在提升出现PPF表现的CTD-ILD的临床认识,为临床决策及管理提供依据,提高临床救治水平。 展开更多
关键词 间质性肺疾病 进展性肺纤维化 结缔组织疾病相关间质性肺疾病 共识
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仿生类器官芯片——多学科交叉研究肺纤维化疾病
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作者 孙伟 赵芸慕兰 +2 位作者 侯文钰 王平 徐作军 《实用医院临床杂志》 2024年第2期8-12,共5页
肺纤维化是肺间质单元内肺泡上皮细胞、肺间质固有成纤维细胞、巨噬细胞、炎性细胞、血管内皮细胞与周细胞等多种纤维化行为共同驱动了肺纤维化进展。受限于目前传统的研究技术,体外试验与动物模型均不能对肺脏各型细胞的生物学行为进... 肺纤维化是肺间质单元内肺泡上皮细胞、肺间质固有成纤维细胞、巨噬细胞、炎性细胞、血管内皮细胞与周细胞等多种纤维化行为共同驱动了肺纤维化进展。受限于目前传统的研究技术,体外试验与动物模型均不能对肺脏各型细胞的生物学行为进行实时观察与监测;传统的体外细胞培养模式多与人体肺间质单元的微环境相差甚远,也无法满足研究需求。仿生类器官芯片技术的迅速发展使体外模拟“肺间质”及充分研究“肺纤维化”成为可能。所以本文重点综述类器官芯片的发展及在肺脏模型中的变革与应用,为充分研究肺纤维化疾病提供全新技术平台与检测思路。 展开更多
关键词 间质性肺疾病 肺纤维化 仿生类器官芯片 肺泡上皮细胞 成纤维细胞
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