It is essential to prepare highly-efficiency reproducible adsorbent for purifying industrial dye wastewater. In this work, biscuit with a layered porous structure as a template is applied to prepare a photocatalytic r...It is essential to prepare highly-efficiency reproducible adsorbent for purifying industrial dye wastewater. In this work, biscuit with a layered porous structure as a template is applied to prepare a photocatalytic recyclable adsorbent of BiFeO3/Carbon nanocomposites for purifying simulative industrial dye wastewater. It is found that the structure of the prepared BiFeO3/Carbon nanocomposite is related to the natural structure of the biscuit, annealing temperatures and immersing times, demonstrated by XRD, TEM, UV-Vis and adsorptive activities. Kinetics data shows that the adsorption rate of the adsorbent to the dye is rapid and fitted well with the pseudo-second-order model, that more than 80% of dyes can be removed in the beginning 30 min. The adsorption isotherm can be perfectly described by the Langmuir model as well. It can be seen from the adsorption data that the adsorption performance can reach over 90% at pH ? 2–12, which can imply its universal utilization. The prepared BiFeO_3/Carbon nanocomposites have also displayed excellent capacities(over 90% within 30 min) for adsorption of seven different dyes and their mixed one. According to the five times photocatalytic reproducible experiments, it is proved that BiFeO_3/Carbon nanocomposites show the excellent stability and reproduction for purifying simulative industrial dyes, even the sample have been placed for one year. These research results indicate that the adsorbent BiFeO_3/Carbon can be a suitable material used in treating industrial dye wastewater potentially.展开更多
The mechanical properties of metal yttrium such as strength, plasticity, hardness and elasticity were introduced . The purifying techniques of yttrium were discussed in detail. The processing methods for metal yttrium...The mechanical properties of metal yttrium such as strength, plasticity, hardness and elasticity were introduced . The purifying techniques of yttrium were discussed in detail. The processing methods for metal yttrium including extruding, forging, rolling, wiredrawing and welding were also introduced. Finally, the potential use of yttrium and its alloys were prospected.展开更多
This article discusses the purifying method of the clayey diatomite or clay ——bearing diatotnite minerals found in China. And the processes of acidizing, beneficiation and gravitational fluidized dry sepa-ration are...This article discusses the purifying method of the clayey diatomite or clay ——bearing diatotnite minerals found in China. And the processes of acidizing, beneficiation and gravitational fluidized dry sepa-ration are reviewed.展开更多
We propose a scheme for purification of a general mixed entangled state. In this scheme, we start from a large number of general mixed entangled states and end up, after local operation and classical communication, wi...We propose a scheme for purification of a general mixed entangled state. In this scheme, we start from a large number of general mixed entangled states and end up, after local operation and classical communication, with a smaller number of Bell diagonal states with higher entanglement. In particular, the scheme can purify one maximally entangled state from two entangled pairs prepared in a class of mixed entangled state. Furthermore we propose a linear optical implementation of the present scheme with polarization beam splitters and photon detectors.展开更多
Next-generation sequencing technology has transformed our ability to assess the taxonomic composition functions of host-associated microbiota and microbiomes. More human microbiome research projects—particularly thos...Next-generation sequencing technology has transformed our ability to assess the taxonomic composition functions of host-associated microbiota and microbiomes. More human microbiome research projects—particularly those that explore genomic mutations within the microbiome—will be launched in the next decade. This review focuses on the coevolution of microbes within a microbiome, which shapes strain-level diversity both within and between host species. We also explore the correlation between microbial genomic mutations and common metabolic diseases, and the adaptive evolution of pathogens and probiotics during invasion and colonization. Finally, we discuss advances in methods and algorithms for annotating and analyzing microbial genomic mutations.展开更多
The distribution of the magnetic flux density in the magnetic field generator which provided travelling magnetic field was determined. The experiments using liquid gallium and aluminum silicon alloy to observe the ...The distribution of the magnetic flux density in the magnetic field generator which provided travelling magnetic field was determined. The experiments using liquid gallium and aluminum silicon alloy to observe the turbulent flow or remove inclusions were performed to obtain the basic principles how the distribution of the magnetic flux density took effect on removing inclusions from molten metal by electromagnetic field. The suitable area in the field for purifying metal was suggested.展开更多
The welding fume has significant effects on the weld workers' health. In order to overwhelm the shortcomings of the control measures of the welding fume, the research of the purifying of welding fume on the spot was ...The welding fume has significant effects on the weld workers' health. In order to overwhelm the shortcomings of the control measures of the welding fume, the research of the purifying of welding fume on the spot was carried out. The purifying device was designed. The relationship between the corona discharge voltage and the purifying efficiency was investigated through experiments with different needle electrode numbers, different ring electrode numbers and different needle-plate distances. The results show that 5-needle double-ring electrode has the best efficiency; the purifying efficiency is increase with the lessening of the needle-plate distance and the increase of the discharge voltage. Using 5-needle double-ring electrode, the purifying efficiency reaches its maximum value 97.9% while the needle-plate distance is 6 cm, the discharge voltage is -35 kV. The purifying device has an excellent purifying effect on the welding fume.展开更多
Genetic load and inbreeding are recognized as important factors to be considered in conservation programs.Elevated levels of both can increase the risk of population extinction by negatively impacting fitness-related ...Genetic load and inbreeding are recognized as important factors to be considered in conservation programs.Elevated levels of both can increase the risk of population extinction by negatively impacting fitness-related characters in many species of plants and animals,including humans(inbreeding depression).Genomic tech-niques are increasingly used in measuring and understanding genetic load and inbreeding and their importance in evolution and conservation.We used whole genome resequencing data from two sibling grouse species in subarctic Eurasia to quantify both.We found a large range of inbreeding measured as FROH(fraction of runs of homozygosity)in individuals from different populations of Chinese Grouse(Tetrastes sewerzowi)and Hazel Grouse(T.bonasia).FROH estimated from genome-wide runs of homozygosity(ROH)ranged from 0.02 to 0.24 among Chinese Grouse populations and from 0.01 to 0.44 in Hazel Grouse.Individuals from a population of Chinese Grouse residing in the Qilian mountains and from the European populations of Hazel Grouse(including samples from Sweden,Germany and Northeast Poland)were the most inbred(FROH ranged from 0.10 to 0.23 and 0.11 to 0.44,respectively).These levels are comparable to other highly inbred populations of birds.Hazel Grouse from northern China and Chinese Grouse residing in the Qinghai-Tibetan Plateau showed relatively lower inbreeding levels.Comparisons of the ratio between deleterious missense mutations and synonymous mutations revealed higher levels in Chinese Grouse as compared to Hazel Grouse.These results are possibly explained by higher fixation rates,mutational melt down,in the range-restricted Chinese Grouse compared to the wide-ranging Hazel Grouse.However,when we compared the relatively more severe class of loss-of-function muta-tions,Hazel Grouse had slightly higher levels than Chinese Grouse,a result which may indicate that purifying selection(purging)has been more efficient in Chinese Grouse on this class of mutations.展开更多
[Objective]The aim of this study was to reveal the molecular evolutionary characteristics of dihydroflavonol 4-Reductase(DFR)—a key enzyme in anthocyanin synthesis pathway in Lamiidae plants.[Method]Based on DFR am...[Objective]The aim of this study was to reveal the molecular evolutionary characteristics of dihydroflavonol 4-Reductase(DFR)—a key enzyme in anthocyanin synthesis pathway in Lamiidae plants.[Method]Based on DFR amino acid and cDNA sequences released by NCBI,molecular evolution of DFR gene was analyzed with bioinformatic software.[Result]Seventeen plants fell into two major branches,Lamiidae and Liliidae.Most of the plants within a same family cluster assembled together,which basically accords with the morphological development of species.Prediction results based on selection pressure using MEGA,DNAsp and PAML software showed that DFR gene in Lamiidae plants underwent strong purifying selection pressure.[Conclusion]Our results laid basis for analyzing the molecular mechanism of enzymatic characteristics of DFR and of the anthocyanin synthesis pathway.展开更多
The application of indium requires high purity indium as material.5N high purity indium had been prepared by the method of acombination of chemically smelting and electrolysis. Smelting timewas 10 min, the abstraction...The application of indium requires high purity indium as material.5N high purity indium had been prepared by the method of acombination of chemically smelting and electrolysis. Smelting timewas 10 min, the abstraction rate of cadmium was 80/100-90/100 whenused solution of I_2-KI and glycerine to smelt indium. 4N metalindium was used as anode, high purity indium as cathode, In-2(SO_4)_3-H_2SO_4 system as electrolyte, and In content is 100 g/L, pH2-3 and current density 80-100 a/m^2.展开更多
Two modes of regulating the water quality of experimental ponds in indoor raceway culture of Litopenaeus vannamei were evaluated using simple water treatment facilities. A self-made water purifying net, aeration stone...Two modes of regulating the water quality of experimental ponds in indoor raceway culture of Litopenaeus vannamei were evaluated using simple water treatment facilities. A self-made water purifying net, aeration stone, composite microbe preparation, and Ceratophyllum demersum were placed in the experimental ponds and the culture water was circulated along the raceway inside the pond using a paddle wheel aerator. In addition, the water quality in the experimental pond was improved by draining effluent from the pipeline at the bottom of ponds 7 and 8 (mode I) and exchanging the circulating water in pond 10 (mode Ⅱ) with the reservoir water in pond 9 using a pump and pipeline. The water quality in the experimental ponds was similar in response to regulation using mode Ⅰ or mode Ⅱ. Water quality parameters in the experimental ponds were controlled within a suitable range by simple facilities during culture period without using any chemical treatments. The rich content of dissolved oxygen was maintained by the circular flow and continuous aeration of the pond water. The respective average values of the main water parameters in experimental ponds 7 and 10 in response to regulation of the water quality using modes Ⅰ and Ⅱwere as follows: pH 8.17 and 7.99; DO 5.16 mg/L and 5.97 mg/L; CODMn18.45 and 12.61 mg/L; TAN (NH3-N) 0.854 mg/L (0.087 mg/L) and 0.427 mg/L (0.012 mg/L); NO2-N 0.489 mg/L and 0.337 mg/L. Moreover, the average body length and body weight of harvested shrimp of pond 7 and pond l0 were 7.56 cm and 8.99 cm, 5.10 g and 8.33 g, respectively. Furthermore, the survival rate, average biomass yield and average condition factor of the shrimp harvested were 70% and 60%, 2.54 kg/m2 and 2.14 kg/m2, and 0.675 g/cm and 0.927 g/cm, respectively. Linear equations describing the relationship between body length and culture time and cubic or power functions describing the relationship between body weight and body length were obtained based on evaluation of the growth data of shrimps throughout the culture period.展开更多
The effect of rare earths(RE) on purifying molten steels, modifying inclusion and micro-alloying are studied in J55 steel. The results show that RE improves the transverse impact energy and increases the resistance of...The effect of rare earths(RE) on purifying molten steels, modifying inclusion and micro-alloying are studied in J55 steel. The results show that RE improves the transverse impact energy and increases the resistance of perforation cracking. The extent on modifying MnS and Al2O3 inclusions is dependent on the activity ratios of RE to Mn and RE to Al. The(a(RE). a(s))/(a(o) . a(Al)) value determines the relative amount of REAlO(3) and RE(2)O(2)S and the properties of steel. RE segregation on grain boundary reduces the segregation of phosphorus and sulfur there.展开更多
The ribosomal proteins are crucial for the maintenance of ribosomal translational efficiency and fidelity.In the study,we characterized the ribosomal protein S30(RPS30)gene from Arachis hypogaea that has been isolated...The ribosomal proteins are crucial for the maintenance of ribosomal translational efficiency and fidelity.In the study,we characterized the ribosomal protein S30(RPS30)gene from Arachis hypogaea that has been isolated through Genefishing analysis during defense responses to Ralstonia solanacearum.The cDNA of RPS 30 contained a 189 base pair(bp)open-reading frame encoding 62 amino acids.The genomic DNA consists of 272 bp containing two exons and one 83 bp intron.The RPS 30 mRNA transcript was mainly expressed in roots and leaves.The expression level of the RPS 30 mRNA transcripts was up-regulated sharply 6 h after bacterial challenge and was 12 times greater than that of the control group.The phylogenetic analysis for genes encoding proteins showed that RPS30 were conserved within dicotyledonous and monocotyledonous plants.d S extremely exceeded d N in all branches of the tree(d N/d S<1.0),indicating that functional constraint have acted on RPS 30 throughout evolution.展开更多
The advanced hot-rolled transformation induced plasticity (TRIP) steel with high tensile strength of 775 MPa, high elongation of 29% and low ductile-brittle transmission temperature less than -80℃ has been develope...The advanced hot-rolled transformation induced plasticity (TRIP) steel with high tensile strength of 775 MPa, high elongation of 29% and low ductile-brittle transmission temperature less than -80℃ has been developed in laboratory based on the common silicon and manganese chemical composition. The experimental results showed that the microstructures were featured with ultra fine grain size less than 5μm, appropriate retained austenite volume fraction around 11.8% and purified ferrite grain, which may result in well balanced mechanical properties for the steel.展开更多
The air microbial species and quantities in a wood mill in Harbin, China weremeasured using sedimentation plate method. Results showed that the microbial quantity in the air atthe workshop without depurator (54939 cfu...The air microbial species and quantities in a wood mill in Harbin, China weremeasured using sedimentation plate method. Results showed that the microbial quantity in the air atthe workshop without depurator (54939 cfu·m^(-3)) was 2.1 times that of the workshop withdepu-rator (25768 cfu·m^(-3)). The depurator could purify air microorganisms at the workshop, witha purifying rate of 53.1%, but it did not reach the standards of clean air. Comparatively thedepurator is effective in reducing the quantity of air actinomyces, and some kinds of airactinomyces, such as Scabies, Cinereas and Hygroscopicas, can be clean out, but it is not veryeffective to bacteria and fungi. It is suggested that more effective and feasible methods should bedeveloped for purifying air microorganisms at the workshop in the future.展开更多
Comparisons of vertebrate mitochondrial genomes(mitogenomes)may yield significant insights into the evolution of organisms and genomes.However,no complete mitogenome from the snake family Psammophiidae has been report...Comparisons of vertebrate mitochondrial genomes(mitogenomes)may yield significant insights into the evolution of organisms and genomes.However,no complete mitogenome from the snake family Psammophiidae has been reported.In this study,we sequenced and annotated the complete mitogenome of Psammophis lineolatus,representing the first mitogenome of Psammophiidae.The total length is 17166 bp,consisting of 13 protein-coding genes(PCGs),22 transfer RNAs(tRNAs),two ribosomal RNAs(12 S rRNA and16 S rRNA),and duplicate control regions(CRs).This gene arrangement belongs to the TypeⅢpattern,which is a widely shared gene order in Alethinophidian snakes.All tRNAs exhibit cloverleaf structures with the exception of tRNA-Ser^(AGY) and tRNA-Pro,which lack a dihydrouridine(DHU)arm/stem and TΨC loop,respectively.The 13 PCGs include five start codons(ATG,GTG,ATA,ATT,and ATC),two complete stop codons(TAA and AGG),and two incomplete stop codon(T--and TA-).In addition,the Ka/Ks ratios indicate that all PCGs had undergone a strong purifying selection.Four types of CR domains rearrangement occurred among eight species of Elapoidea.The phylogenetic reconstructions with both Bayesian inference and maximum likelihood methods support the placement of Psammophiidae in the Elapoidea superfamily,with Homalopsidae being the sister taxon to Elapoidea and Colubroidea.However,the sister taxon of Psammophiidae is unclear due to the availability of Elapoidea mitogenomes being limited to the family Elapidae.More mitogenomes from different taxonomic groups in Elapoidea are needed to better understand the phylogenetic relationships within Elapoidea.展开更多
Pure Cu films and Cu alloy films containing insoluble substances(Zr and Cr)were deposited on Si(100)substrates,in the presence of interfacial native suboxide(SiOx),by magnetron sputtering.Samples were vacuum annealed ...Pure Cu films and Cu alloy films containing insoluble substances(Zr and Cr)were deposited on Si(100)substrates,in the presence of interfacial native suboxide(SiOx),by magnetron sputtering.Samples were vacuum annealed between 300℃and 500 ℃to investigate effects of Zr and Cr additions on the thermal performance of Cu films.After annealing,copper silicides were found in the Cu(Zr)films,while no detectable silicides were observed in Cu and Cu(Cr)films.Upon annealing,Zr accelerated the diffusion and reaction between the film and the substrate,and lowered the thermal stability of Cu(Zr)alloy films on Si substrates,which was ascribed to the‘purifying effect’of Zr on the Si substrates.Whereas,Cr prohibited the agglomeration of Cu films at 500℃and decreased the surface roughness.As a result,the diffusion of Cu in Si substrates for Cu(Cr)films was effectively inhibited.In contrast to the high resistivity of Cu(Zr)films,the final resistivity of about 2.76μΩ·cm was achieved for the Cu(Cr)film.These results indicate that Cu(Cr)films have higher thermal stability than Cu(Zr)films on Si substrates and are preferable in the advanced barrierless Cu metallization.展开更多
The major histocompatibility complex (MHC) is a dynamic genetic region with an essential role in the adaptive immunity of jawed vertebrates. The MHC polymorphism is affected by many processes such as birth-and- deat...The major histocompatibility complex (MHC) is a dynamic genetic region with an essential role in the adaptive immunity of jawed vertebrates. The MHC polymorphism is affected by many processes such as birth-and- death evolution, gene conversion, and concerted evolution. Studies investigating the evolution of MHC class I genes have been biased toward a few particular taxa and model species. However, the investigation of this region in nonavian reptiles is still in its infancy. We present the first characterization of MHC class I genes in a species from the family Lacertidae. We assessed genetic diversity and a role of selection in shaping the diversity of MHC class I exon 4 among 37 individuals of Eremias multiocellata from a population in Lanzhou, China. We generated 67 distinct DNA sequences using cloning and sequencing methods, and identified 36 putative functional variants as well as two putative pseudogene-variants. We found the number of variants within an individual varying between two and seven, indicating that there are at least four MHC class I loci in this species. Gene duplication plays a role in increasing copy numbers of MHC genes and allelic diversity in this species. The class I exon 4 sequences are characteristic of low nucleotide diversity. No signal of recombination is detected, but purifying selection is detected in β2-microglobulin interaction sites and some other silent sites outside of the function-constraint regions. Certain identical alleles are shared by Eremias multiocellata and E. przewalskii and E. brenchleyi, suggesting trans-species polymorphism. The data are compatible with a birth-and-death model of evolution.展开更多
基金financial support from the NSFC project(21501052 and 91622119)the China Postdoctoral Science Foundation(2015M570304)+2 种基金Special Funding for Postdoctoral of Heilongjiang Province(LBH-TZ06019)the Science Foundation for Excellent Youth of Harbin City of China(2016RQQXJ099)UNPYSCT-2016173
文摘It is essential to prepare highly-efficiency reproducible adsorbent for purifying industrial dye wastewater. In this work, biscuit with a layered porous structure as a template is applied to prepare a photocatalytic recyclable adsorbent of BiFeO3/Carbon nanocomposites for purifying simulative industrial dye wastewater. It is found that the structure of the prepared BiFeO3/Carbon nanocomposite is related to the natural structure of the biscuit, annealing temperatures and immersing times, demonstrated by XRD, TEM, UV-Vis and adsorptive activities. Kinetics data shows that the adsorption rate of the adsorbent to the dye is rapid and fitted well with the pseudo-second-order model, that more than 80% of dyes can be removed in the beginning 30 min. The adsorption isotherm can be perfectly described by the Langmuir model as well. It can be seen from the adsorption data that the adsorption performance can reach over 90% at pH ? 2–12, which can imply its universal utilization. The prepared BiFeO_3/Carbon nanocomposites have also displayed excellent capacities(over 90% within 30 min) for adsorption of seven different dyes and their mixed one. According to the five times photocatalytic reproducible experiments, it is proved that BiFeO_3/Carbon nanocomposites show the excellent stability and reproduction for purifying simulative industrial dyes, even the sample have been placed for one year. These research results indicate that the adsorbent BiFeO_3/Carbon can be a suitable material used in treating industrial dye wastewater potentially.
文摘The mechanical properties of metal yttrium such as strength, plasticity, hardness and elasticity were introduced . The purifying techniques of yttrium were discussed in detail. The processing methods for metal yttrium including extruding, forging, rolling, wiredrawing and welding were also introduced. Finally, the potential use of yttrium and its alloys were prospected.
文摘This article discusses the purifying method of the clayey diatomite or clay ——bearing diatotnite minerals found in China. And the processes of acidizing, beneficiation and gravitational fluidized dry sepa-ration are reviewed.
文摘We propose a scheme for purification of a general mixed entangled state. In this scheme, we start from a large number of general mixed entangled states and end up, after local operation and classical communication, with a smaller number of Bell diagonal states with higher entanglement. In particular, the scheme can purify one maximally entangled state from two entangled pairs prepared in a class of mixed entangled state. Furthermore we propose a linear optical implementation of the present scheme with polarization beam splitters and photon detectors.
基金supported by the National Natural Science Foundation of China(31701577).
文摘Next-generation sequencing technology has transformed our ability to assess the taxonomic composition functions of host-associated microbiota and microbiomes. More human microbiome research projects—particularly those that explore genomic mutations within the microbiome—will be launched in the next decade. This review focuses on the coevolution of microbes within a microbiome, which shapes strain-level diversity both within and between host species. We also explore the correlation between microbial genomic mutations and common metabolic diseases, and the adaptive evolution of pathogens and probiotics during invasion and colonization. Finally, we discuss advances in methods and algorithms for annotating and analyzing microbial genomic mutations.
文摘The distribution of the magnetic flux density in the magnetic field generator which provided travelling magnetic field was determined. The experiments using liquid gallium and aluminum silicon alloy to observe the turbulent flow or remove inclusions were performed to obtain the basic principles how the distribution of the magnetic flux density took effect on removing inclusions from molten metal by electromagnetic field. The suitable area in the field for purifying metal was suggested.
文摘The welding fume has significant effects on the weld workers' health. In order to overwhelm the shortcomings of the control measures of the welding fume, the research of the purifying of welding fume on the spot was carried out. The purifying device was designed. The relationship between the corona discharge voltage and the purifying efficiency was investigated through experiments with different needle electrode numbers, different ring electrode numbers and different needle-plate distances. The results show that 5-needle double-ring electrode has the best efficiency; the purifying efficiency is increase with the lessening of the needle-plate distance and the increase of the discharge voltage. Using 5-needle double-ring electrode, the purifying efficiency reaches its maximum value 97.9% while the needle-plate distance is 6 cm, the discharge voltage is -35 kV. The purifying device has an excellent purifying effect on the welding fume.
基金funded by the National Natural Science Foundation of China(NSFC,Grant No.31520103903 to Y.-H.Sun and J.Hoglund)the Biodiversity Conservation Project of the Second Forest and Grass Ecosystem Recovery(Grant No.QHTX-2021-016).
文摘Genetic load and inbreeding are recognized as important factors to be considered in conservation programs.Elevated levels of both can increase the risk of population extinction by negatively impacting fitness-related characters in many species of plants and animals,including humans(inbreeding depression).Genomic tech-niques are increasingly used in measuring and understanding genetic load and inbreeding and their importance in evolution and conservation.We used whole genome resequencing data from two sibling grouse species in subarctic Eurasia to quantify both.We found a large range of inbreeding measured as FROH(fraction of runs of homozygosity)in individuals from different populations of Chinese Grouse(Tetrastes sewerzowi)and Hazel Grouse(T.bonasia).FROH estimated from genome-wide runs of homozygosity(ROH)ranged from 0.02 to 0.24 among Chinese Grouse populations and from 0.01 to 0.44 in Hazel Grouse.Individuals from a population of Chinese Grouse residing in the Qilian mountains and from the European populations of Hazel Grouse(including samples from Sweden,Germany and Northeast Poland)were the most inbred(FROH ranged from 0.10 to 0.23 and 0.11 to 0.44,respectively).These levels are comparable to other highly inbred populations of birds.Hazel Grouse from northern China and Chinese Grouse residing in the Qinghai-Tibetan Plateau showed relatively lower inbreeding levels.Comparisons of the ratio between deleterious missense mutations and synonymous mutations revealed higher levels in Chinese Grouse as compared to Hazel Grouse.These results are possibly explained by higher fixation rates,mutational melt down,in the range-restricted Chinese Grouse compared to the wide-ranging Hazel Grouse.However,when we compared the relatively more severe class of loss-of-function muta-tions,Hazel Grouse had slightly higher levels than Chinese Grouse,a result which may indicate that purifying selection(purging)has been more efficient in Chinese Grouse on this class of mutations.
基金Supported by the Fundamental Research Funds for the Central Universities(SWJTU09BR208)~~
文摘[Objective]The aim of this study was to reveal the molecular evolutionary characteristics of dihydroflavonol 4-Reductase(DFR)—a key enzyme in anthocyanin synthesis pathway in Lamiidae plants.[Method]Based on DFR amino acid and cDNA sequences released by NCBI,molecular evolution of DFR gene was analyzed with bioinformatic software.[Result]Seventeen plants fell into two major branches,Lamiidae and Liliidae.Most of the plants within a same family cluster assembled together,which basically accords with the morphological development of species.Prediction results based on selection pressure using MEGA,DNAsp and PAML software showed that DFR gene in Lamiidae plants underwent strong purifying selection pressure.[Conclusion]Our results laid basis for analyzing the molecular mechanism of enzymatic characteristics of DFR and of the anthocyanin synthesis pathway.
基金This project is financially supported by Chinese Key Project of Science and Technology (No.96-119-04-01)
文摘The application of indium requires high purity indium as material.5N high purity indium had been prepared by the method of acombination of chemically smelting and electrolysis. Smelting timewas 10 min, the abstraction rate of cadmium was 80/100-90/100 whenused solution of I_2-KI and glycerine to smelt indium. 4N metalindium was used as anode, high purity indium as cathode, In-2(SO_4)_3-H_2SO_4 system as electrolyte, and In content is 100 g/L, pH2-3 and current density 80-100 a/m^2.
基金Supported by the Shanghai Commission of Science and Technology (No.063919112073919102)the Shanghai Agricultural Committee (No.2006,9-4)
文摘Two modes of regulating the water quality of experimental ponds in indoor raceway culture of Litopenaeus vannamei were evaluated using simple water treatment facilities. A self-made water purifying net, aeration stone, composite microbe preparation, and Ceratophyllum demersum were placed in the experimental ponds and the culture water was circulated along the raceway inside the pond using a paddle wheel aerator. In addition, the water quality in the experimental pond was improved by draining effluent from the pipeline at the bottom of ponds 7 and 8 (mode I) and exchanging the circulating water in pond 10 (mode Ⅱ) with the reservoir water in pond 9 using a pump and pipeline. The water quality in the experimental ponds was similar in response to regulation using mode Ⅰ or mode Ⅱ. Water quality parameters in the experimental ponds were controlled within a suitable range by simple facilities during culture period without using any chemical treatments. The rich content of dissolved oxygen was maintained by the circular flow and continuous aeration of the pond water. The respective average values of the main water parameters in experimental ponds 7 and 10 in response to regulation of the water quality using modes Ⅰ and Ⅱwere as follows: pH 8.17 and 7.99; DO 5.16 mg/L and 5.97 mg/L; CODMn18.45 and 12.61 mg/L; TAN (NH3-N) 0.854 mg/L (0.087 mg/L) and 0.427 mg/L (0.012 mg/L); NO2-N 0.489 mg/L and 0.337 mg/L. Moreover, the average body length and body weight of harvested shrimp of pond 7 and pond l0 were 7.56 cm and 8.99 cm, 5.10 g and 8.33 g, respectively. Furthermore, the survival rate, average biomass yield and average condition factor of the shrimp harvested were 70% and 60%, 2.54 kg/m2 and 2.14 kg/m2, and 0.675 g/cm and 0.927 g/cm, respectively. Linear equations describing the relationship between body length and culture time and cubic or power functions describing the relationship between body weight and body length were obtained based on evaluation of the growth data of shrimps throughout the culture period.
文摘The effect of rare earths(RE) on purifying molten steels, modifying inclusion and micro-alloying are studied in J55 steel. The results show that RE improves the transverse impact energy and increases the resistance of perforation cracking. The extent on modifying MnS and Al2O3 inclusions is dependent on the activity ratios of RE to Mn and RE to Al. The(a(RE). a(s))/(a(o) . a(Al)) value determines the relative amount of REAlO(3) and RE(2)O(2)S and the properties of steel. RE segregation on grain boundary reduces the segregation of phosphorus and sulfur there.
基金Natural Science Funds for Young Scholar of Shandong Academy of Agricultural Sciences,China(No.2015YQN13)Natural Science Foundation of Shandong Province,China(ZR2015YL064)+2 种基金Qingdao Science and Technology Plan Basic Research Project,China(No.12-1-4-11-(1)-jch)China Agricultural Research System(No.CARS-13)Agricultural Scientific and Technological Innovation Project of Shandong Academy of Agricultural Sciences,China(No.CXGC2018E21)
文摘The ribosomal proteins are crucial for the maintenance of ribosomal translational efficiency and fidelity.In the study,we characterized the ribosomal protein S30(RPS30)gene from Arachis hypogaea that has been isolated through Genefishing analysis during defense responses to Ralstonia solanacearum.The cDNA of RPS 30 contained a 189 base pair(bp)open-reading frame encoding 62 amino acids.The genomic DNA consists of 272 bp containing two exons and one 83 bp intron.The RPS 30 mRNA transcript was mainly expressed in roots and leaves.The expression level of the RPS 30 mRNA transcripts was up-regulated sharply 6 h after bacterial challenge and was 12 times greater than that of the control group.The phylogenetic analysis for genes encoding proteins showed that RPS30 were conserved within dicotyledonous and monocotyledonous plants.d S extremely exceeded d N in all branches of the tree(d N/d S<1.0),indicating that functional constraint have acted on RPS 30 throughout evolution.
文摘The advanced hot-rolled transformation induced plasticity (TRIP) steel with high tensile strength of 775 MPa, high elongation of 29% and low ductile-brittle transmission temperature less than -80℃ has been developed in laboratory based on the common silicon and manganese chemical composition. The experimental results showed that the microstructures were featured with ultra fine grain size less than 5μm, appropriate retained austenite volume fraction around 11.8% and purified ferrite grain, which may result in well balanced mechanical properties for the steel.
文摘The air microbial species and quantities in a wood mill in Harbin, China weremeasured using sedimentation plate method. Results showed that the microbial quantity in the air atthe workshop without depurator (54939 cfu·m^(-3)) was 2.1 times that of the workshop withdepu-rator (25768 cfu·m^(-3)). The depurator could purify air microorganisms at the workshop, witha purifying rate of 53.1%, but it did not reach the standards of clean air. Comparatively thedepurator is effective in reducing the quantity of air actinomyces, and some kinds of airactinomyces, such as Scabies, Cinereas and Hygroscopicas, can be clean out, but it is not veryeffective to bacteria and fungi. It is suggested that more effective and feasible methods should bedeveloped for purifying air microorganisms at the workshop in the future.
基金supported by the National Natural Science Foundation of China(Nos.31672270,32070433)supported by grant from the National Natural Science Foundation of China(No.31560591)。
文摘Comparisons of vertebrate mitochondrial genomes(mitogenomes)may yield significant insights into the evolution of organisms and genomes.However,no complete mitogenome from the snake family Psammophiidae has been reported.In this study,we sequenced and annotated the complete mitogenome of Psammophis lineolatus,representing the first mitogenome of Psammophiidae.The total length is 17166 bp,consisting of 13 protein-coding genes(PCGs),22 transfer RNAs(tRNAs),two ribosomal RNAs(12 S rRNA and16 S rRNA),and duplicate control regions(CRs).This gene arrangement belongs to the TypeⅢpattern,which is a widely shared gene order in Alethinophidian snakes.All tRNAs exhibit cloverleaf structures with the exception of tRNA-Ser^(AGY) and tRNA-Pro,which lack a dihydrouridine(DHU)arm/stem and TΨC loop,respectively.The 13 PCGs include five start codons(ATG,GTG,ATA,ATT,and ATC),two complete stop codons(TAA and AGG),and two incomplete stop codon(T--and TA-).In addition,the Ka/Ks ratios indicate that all PCGs had undergone a strong purifying selection.Four types of CR domains rearrangement occurred among eight species of Elapoidea.The phylogenetic reconstructions with both Bayesian inference and maximum likelihood methods support the placement of Psammophiidae in the Elapoidea superfamily,with Homalopsidae being the sister taxon to Elapoidea and Colubroidea.However,the sister taxon of Psammophiidae is unclear due to the availability of Elapoidea mitogenomes being limited to the family Elapidae.More mitogenomes from different taxonomic groups in Elapoidea are needed to better understand the phylogenetic relationships within Elapoidea.
基金Project(08520740200)supported by the Applied Materials Research and Development Fund of Shanghai,China
文摘Pure Cu films and Cu alloy films containing insoluble substances(Zr and Cr)were deposited on Si(100)substrates,in the presence of interfacial native suboxide(SiOx),by magnetron sputtering.Samples were vacuum annealed between 300℃and 500 ℃to investigate effects of Zr and Cr additions on the thermal performance of Cu films.After annealing,copper silicides were found in the Cu(Zr)films,while no detectable silicides were observed in Cu and Cu(Cr)films.Upon annealing,Zr accelerated the diffusion and reaction between the film and the substrate,and lowered the thermal stability of Cu(Zr)alloy films on Si substrates,which was ascribed to the‘purifying effect’of Zr on the Si substrates.Whereas,Cr prohibited the agglomeration of Cu films at 500℃and decreased the surface roughness.As a result,the diffusion of Cu in Si substrates for Cu(Cr)films was effectively inhibited.In contrast to the high resistivity of Cu(Zr)films,the final resistivity of about 2.76μΩ·cm was achieved for the Cu(Cr)film.These results indicate that Cu(Cr)films have higher thermal stability than Cu(Zr)films on Si substrates and are preferable in the advanced barrierless Cu metallization.
基金supported by the Science and Technology Project for Outstanding Youths in Life Science (KSCX2-EW-Q-6) from the Chinese Academy of SciencesNational Natural Science Foundation of China (31272281)
文摘The major histocompatibility complex (MHC) is a dynamic genetic region with an essential role in the adaptive immunity of jawed vertebrates. The MHC polymorphism is affected by many processes such as birth-and- death evolution, gene conversion, and concerted evolution. Studies investigating the evolution of MHC class I genes have been biased toward a few particular taxa and model species. However, the investigation of this region in nonavian reptiles is still in its infancy. We present the first characterization of MHC class I genes in a species from the family Lacertidae. We assessed genetic diversity and a role of selection in shaping the diversity of MHC class I exon 4 among 37 individuals of Eremias multiocellata from a population in Lanzhou, China. We generated 67 distinct DNA sequences using cloning and sequencing methods, and identified 36 putative functional variants as well as two putative pseudogene-variants. We found the number of variants within an individual varying between two and seven, indicating that there are at least four MHC class I loci in this species. Gene duplication plays a role in increasing copy numbers of MHC genes and allelic diversity in this species. The class I exon 4 sequences are characteristic of low nucleotide diversity. No signal of recombination is detected, but purifying selection is detected in β2-microglobulin interaction sites and some other silent sites outside of the function-constraint regions. Certain identical alleles are shared by Eremias multiocellata and E. przewalskii and E. brenchleyi, suggesting trans-species polymorphism. The data are compatible with a birth-and-death model of evolution.