Low Selenium and Low Protein Exacerbate Myocardial Damage in Keshan Disease by Affecting the PINK1/Parkin-mediated Mitochondrial Autophagy Pathway

Objective Keshan disease(KD)is a myocardial mitochondrial disease closely related to insufficient selenium(Se)and protein intake.PTEN induced putative kinase 1(PINK1)/Parkin mediated mitochondrial autophagy regulates various physiological and pathological processes in the body.This study aimed to elucidate the relationship between PINK1/Parkin-regulated mitochondrial autophagy and KD-related myocardial injury.Methods A low Se and low protein animal model was established.One hundred Wistar rats were randomly divided into 5 groups(control group,low Se group,low protein group,low Se+low protein group,and corn from KD area group).The JC-1 method was used to detect the mitochondrial membrane potential(MMP).ELISA was used to detect serum creatine kinase MB(CK-MB),cardiac troponin I(cTnI),and mitochondrial-glutamicoxalacetic transaminase(M-GOT)levels.RT-PCR and Western blot analysis were used to detect the expression of PINK1,Parkin,sequestome 1(P62),and microtubule-associated proteins1A/1B light chain 3B(MAP1LC3B).Results The MMP was significantly decreased and the activity of CK-MB,cTnI,and M-GOT significantly increased in each experimental group(low Se group,low protein group,low Se+low protein group and corn from KD area group)compared with the control group(P<0.05 for all).The mRNA and protein expression levels of PINK1,Parkin and MAP1LC3B were profoundly increased,and those of P62 markedly decreased in the experimental groups compared with the control group(P<0.05 for all).Conclusion Low Se and low protein levels exacerbate myocardial damage in KD by affecting the PINK1/Parkin-mediated mitochondrial autophagy pathway.

The Concepts of "Putting People First" and "Regarding Harmony as the Fundamentality" under the Framework of Cultural Diversity

Human civilization is currently undergoing another important turn. Following the economic globalization,cultural globalization has become an inevitable trend.

Putting People First and Global Human Rights Governance--A Summary of Academic Viewpoints of“2021 South-South Human Rights Forum”

On December 8, 2021, China’s State Council Information office(SCIo) and Ministry of Foreign Affairs co-hosted the “2021 South-South Human Rights Forum” in Beijing. The forum is themed on “putting people First and Global Human Rights Governance”. Four sub-themes, namely, “Building a Global Community of Health for All and protecting Human Rights”, “Anti-poverty Ffforts and Realization of the Right to Development”, “Multilateralism and Global Human Rights Governance”, and “The Role of Developing Countries in Global Human Rights Governance”, were discussed by delegates from more than 100 countries and international organizations. Within the framework of the theme of the forum, the delegates fully discussed on how to better respect and protect human rights, advance equal cooperation, share development outcomes, seek common and sustainable development, maintain fairness and justice in the international community, and improve the basic experience of the protection of human rights, and so on. As an important platform for the exchanges and cooperation of developing countries in human rights, the South-South Human Rights Forum has effectively promoted mutual understanding, inclusiveness and mutual learning on human rights governance.

A Centennial Journey of Putting People First——“The Communist Party of China and the Progress on Human Rights in China” International Conference Summary

The International Conference on"The Communist Party of China and the Progress on Human Rights in China"was held on April 8,2021.It was sponsored by the China Society for Human Rights Studies and the Publicity Department of the CPC Jilin Provincial Committee and organized by Jilin University Human Rights Center,Jilin University School of Law,and Jilin University Center for Jurisprudence Research.At the conference,representatives from more than 20 countries discussed in depth the achievements,experience,and theories of the Communist Party of China in leading the Chinese people to strive for a better life and promoting the protection of human rights from dimensions of history and reality.The conference fully demonstrated the historical role and significance of the CPC’s path,thoughts,theories,and practices concerning human rights.

Putting Down Roots

Cameroon plans to plant one million trees a year to try and limit the effects of climate change dance of equatorial forests,which make up part of the Congo Basin recognized as the second largest equatorial forest in the world after the Amazon Basin.

Putting Justice First Africa and the ICC must find common ground

It is true that there is a double standard still at work in international justice, however, it is not the ICC but international politics and states＇ perceived political interests that are to blame.

Putting Down Wheels

China donates 100 police vehicles on the 10th anniversary of diplomatic ties with Malawi

Lesson 10 Putting Up at a Hotel

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PTEN-induced kinase 1-induced dynamin-related protein 1 Ser637 phosphorylation reduces mitochondrial fission and protects against intestinal ischemia reperfusion injury

BACKGROUND Intestinal ischemia reperfusion(I/R)occurs in various diseases,such as trauma and intestinal transplantation.Excessive reactive oxygen species(ROS)accumulation and subsequent apoptotic cell death in intestinal epithelia are important causes of I/R injury.PTEN-induced putative kinase 1(PINK1)and phosphorylation of dynamin-related protein 1(DRP1)are critical regulators of ROS and apoptosis.However,the correlation of PINK1 and DRP1 and their function in intestinal I/R injury have not been investigated.Thus,examining the PINK1/DRP1 pathway may help to identify a protective strategy and improve the patient prognosis.AIM To clarify the mechanism of the PINK1/DRP1 pathway in intestinal I/R injury.METHODS Male C57BL/6 mice were used to generate an intestinal I/R model via superior mesenteric artery occlusion followed by reperfusion.Chiu’s score was used to evaluate intestinal mucosa damage.The mitochondrial fission inhibitor mdivi-1 was administered by intraperitoneal injection.Caco-2 cells were incubated in vitro in hypoxia/reoxygenation conditions.Small interfering RNAs and overexpression plasmids were transfected to regulate PINK1 expression.The protein expression levels of PINK1,DRP1,p-DRP1 and cleaved caspase 3 were measured by Western blotting.Cell viability was evaluated using a Cell Counting Kit-8 assay and cell apoptosis was analyzed by TUNEL staining.Mitochondrial fission and ROS were tested by MitoTracker and MitoSOX respectively.RESULTS Intestinal I/R and Caco-2 cell hypoxia/reoxygenation decreased the expression of PINK1 and p-DRP1 Ser637.Pretreatment with mdivi-1 inhibited mitochondrial fission,ROS generation,and apoptosis and ameliorated cell injury in intestinal I/R.Upon PINK1 knockdown or overexpression in vitro,we found that p-DRP1 Ser637 expression and DRP1 recruitment to the mitochondria were associated with PINK1.Furthermore,we verified the physical combination of PINK1 and p-DRP1 Ser637.CONCLUSION PINK1 is correlated with mitochondrial fission and apoptosis by regulating DRP1 phosphorylation in intestinal I/R.These results suggest that the PINK1/DRP1 pathway is involved in intestinal I/R injury,and provide a new approach for prevention and treatment.

Putative roles of cathepsin B in Alzheimer's disease pathology: the good, the bad, and the ugly in one?

Alzheimer＇s disease（AD）is a fatal progressive neurodegenerative disorder characterized by loss in memory,cognition,and executive function and activities of daily living.AD pathogenesis has been shown to involve loss of neurons and synapses,cholinergic deficits,amyloid-beta protein（Aβ）deposition,tau protein hyperphosphorylation, and neuroinflammation.

Genetic diversity in two threatened species in Vietnam:Taxus chinensis and Taxus wallichiana

Taxus chinensis and T. wallichiana in have been threatened in their distribution areas in recent decades because of their over-exploitation and reduction and destruction of native habitats. Determining the genetic diversity in populations of the two species will provide guidelines for their protection and preservation. Two hundred and fifteen trees from six populations of T. chinensis and150 sampled trees of T. wallichiana were sampled. Six microsatellite primer pairs selected from 16 primer pairs were used to investigate genetic variation at the population and species levels. Five yielded polymorphic alleles, and among the 13 putative alleles amplified, 11 were polymorphic（accounting for 76.33 %）.Shannon＇s information index（I） and percentage of polymorphic bands（PPB）（I = 0.202 and PPB = 67.22 % for T. chinensis; I = 0.217 and PPB = 65.03 % for T. wallichiana）. Both species had low levels of genetic diversity（mean Ho= 0.107, He= 0.121 for T. chinensis; Ho= 0.095, He= 0.109 for T. wallichiana）. Genetic differentiation among populations was higher（FST= 0.189） for T. chinensis and lower（0.156） for T.wallichiana, indicating limited gene flow（Nm） among populations for T. chinensis（0.68） and T. wallichiana（0.65）.Variation among individuals of T. chinensis was 63.59 and73.12 % for T. wallichiana. Thus, the threatened status of the two conifers is related to a lack of genetic diversity. All populations are isolated in small forest remnants. An ex situ conservation site should be established with a new population for these species that comprises all the genetic groups for the best chance to improve their fitness under environmental stresses.

Putting Faces to Names： Snapshots of Two Committee Meetings, 95 Years Apart, Emphasize Continuous International Cooperation in the Atmospheric Sciences

1.Introduction Spectacular advances have been made in the atmospheric sciences on a global level during a period of one hundred years or more,which is arguably most evident through"the quiet revolution of numerical weather prediction"(Bauer et

Flank gland-secreted putative chemosignals pertaining to photoperiod,endocrine states,and sociosexual behavior in golden hamsters

Behavioral studies have shown that flank glands are involved in chemical communication in golden hamsters Mesocricetus auratus but little chemical analysis has been conducted on volatiles arising from these glands. Using gas chromatography-mass spectrometry, we detected compounds from the flank glands of males, only eight of which were also produced in females. Based on these chemical data we performed a number of further experiments. By manipulating light we found that males exposed to short-photoperiods developed smaller flank glands than those exposed to long-photoperiods. Six flank gland volatiles reduced in relative abundance, which possibly coded for reproductive status of males of this seasonally breeding hamster species. Through dyadic encounters, we were able to induce the formation of dominant-subordinate relationships and show that two glandular compounds became high in relative abundance and may function as dominance pheromones. Castration eliminated all male-specific compounds resulting from flank glands, but bilateral ovariectomies only affected one compound in females. Once these ovariectomized females were treated with testosterone, their glandular compounds resembled those of males, suggesting these compounds are under the main control of androgen. Two female putative pheromones, tetradeeanoic acid and hexadecanoic acid, were used in binary choice tests and were both found to attract males over females. Applying a solution of these pheromone compounds to adult males also suppressed their agonistic behavior [Current Zoology 56 （6）： 800-812, 2010].

ENVIRONMENTAL CARCINOGENESIS:HOST/GENETIC AND ENVIRONMENTAL FACTORS

Understanding environmental carcinogenesis is criticalfor its effective management through prevention andintervention.

Identification and Characterization of Putative Virulent Genes in Streptococcus equi ssp. zooepidemicus

Suppression subtractive hybridization （SSH） was performed with virulent strain ATCC35246 and avirulent strain ST171 to identify novel genes associated with virulence in Streptococcus equi ssp. zooepidemicus （SEZ）. There were fourteen genomic regions that only presented in virulent strain ATCC35246. These regions encoded 14 proteins, some of them were homologous to proteins associated with cellular surface structure, molecular synthesis, energy metabolism, regulation, transport systems, and other unknown functions. Primers for 6 particular regions were designed from the already published SEZ sequence. Then, we used PCR to evaluate the distribution and conservation of these 6 DNA fragments in various SEZ strains collected from different sources, regions, groups, and times. The results showed that these 6 DNA fragments were widely distributed in SEZ strains, yet they were not existence in the avirulent strain ST171. Moreover, these fragments could not be detected in other Streptococcus groups.

Guangxi Beihai China Resources Cement Shoots at Putting into Production in May 2014

China Resources Cement in Hepu County of Beihai City is building two new type dry method cement production lines with two sets of 9 MW pure low temperatures residual heat power generating systems matched. Phase I of the project will be invested CNY I billion, and had been invested CNY 0.13 billion in 2012. China Resources Cement is shooting at putting the lines into production in May 2014.

Aggressive Exploration and Sustainable Development——ZTE Puts Emphasis on R&D to Forge Competitive Edge

ZTE Corporation released its 2002 annualreport on March 12, 2003, proclaimingthat its main business line revenue andnet profit reached 11 billion RMB and0.57 billion RMB respectively.As a major manufacturer of telecommunica-tion equipment in China, ZTE constantly putsemphasis on R&D so as to forge competitiveedge.Establishing Scientific Management

An Elephant and a Gentleman

At a country house in Belgium,there lived a young elephant (1) was apet (宠物) to the people,it used to (2) the dining-room after dinner andasked for food from the visitors.One day,when visitors were (3) the tablethe elephant came round and put its trunk between the visitors,(4) fruit

My UNDERSTANDING OF METHODOLOGy

with the development of commerce and the increase of foreign affairactivities in our country, English as a communicative means is getting moreand more important It is taken as a re quired course in almost all middleschools and universities The Centlral Committee of the Chinese

Making Up

THE girls in my college were fond of forming cliques,I admit,I was one of them.We dined together,studied together,and if we fell out,factions broke off until we got over whatever grudge we were holding and the gang could reform.In Dorm 315,there were two groups among the six girls：one included five girls,Wu Shasha,Tan Fang,Zeng Li,Liu Siqi and me;the other,had only one member,Lu Xiaolu.We had to admit that