Objective:To abrogate pathogenic vibrios in aquaculture by testing the potential of Pseudomonas isolates from fresh water,brackish and marine environments as probiotics.Methods:Antagonistic activity of the compound ag...Objective:To abrogate pathogenic vibrios in aquaculture by testing the potential of Pseudomonas isolates from fresh water,brackish and marine environments as probiotics.Methods:Antagonistic activity of the compound against 7 Vibrio spp.was performed.Influence of salinity on the production of pyocyanin and the toxicity was done through the compound using brine shrimp lethality assay.Molecular characterization was performed to confirm that the isolates werePseudomonas aeruginosa.Results:Salinity was found to regulate the levels of pyocyanin production,with 5-10 g/L as the optimum.All Pseudomonas isolates grew at salinities ranging from 5 to 70 g/L.Isolates of marine origin produced detectable levels of pyocyanin up to 45 g/L salinity.Brackish and freshwater isolates ceased to produce pyocyanin at salinities above 30 g/L and 20 g/L,respectively.Culture supernatants of all 5 Pseudomonas isolates possessed the ability to restrict the growth of Vibrio spp.and maximum antagonistic effect on Vibrio harveyi was obtained when they were grown at salinities of 5 to 10 g/L.The marine isolate MCCB117,even when grown at a salinity of 45 g/L possessed the ability to inhibit Vibrio spp.Conclusions:Purification and structural elucidation of antagonistic compound were carried out.ideal for application in freshwater,MCCB102 and MCCB103 in brackish water and MCCB117 and The present investigation showed that Pseudomonas aeruginosa MCCB119 would be MCCB118 in marine aquaculture systems as putative probiotics in the management of vibrios.展开更多
The aim of this work was to study the pyocyanin pigment from Pseudomonas aeruginosa DN9.The work involves optimiza-tion of process parameters for enhanced production of pyocyanin pigment under submerged fermentation c...The aim of this work was to study the pyocyanin pigment from Pseudomonas aeruginosa DN9.The work involves optimiza-tion of process parameters for enhanced production of pyocyanin pigment under submerged fermentation condition.During optimization process,maximum pyocyanin production(92.12μg/ml)was obtained with carbon source mannitole,nitrogen source peptone,inorganic salt NaCl and metal ion FeSO_(4).Plackett Burman design and Response Surface Methodology(RSM)showed peptone,NaCl and KH_(2)PO_(4)are significant variable in the production of pyocyanin pigment.The FTIR and GC-MS study was done to evaluate structural properties of pyocyanin pigment.The purified pigment was further analyzed as colouring agent and for inhibitory action against pathogenic microorganisms.Thus,present study showed Pseudomonas aeruginosa DN9 as promising culture for pigment production with potential biotechnological application.展开更多
基金Supported by Department of Biotechnology,Government of India under the project,Program Support in Marine Biotechnology(Grant no.:BT/PR4012/AAQ/03/204/2003).
文摘Objective:To abrogate pathogenic vibrios in aquaculture by testing the potential of Pseudomonas isolates from fresh water,brackish and marine environments as probiotics.Methods:Antagonistic activity of the compound against 7 Vibrio spp.was performed.Influence of salinity on the production of pyocyanin and the toxicity was done through the compound using brine shrimp lethality assay.Molecular characterization was performed to confirm that the isolates werePseudomonas aeruginosa.Results:Salinity was found to regulate the levels of pyocyanin production,with 5-10 g/L as the optimum.All Pseudomonas isolates grew at salinities ranging from 5 to 70 g/L.Isolates of marine origin produced detectable levels of pyocyanin up to 45 g/L salinity.Brackish and freshwater isolates ceased to produce pyocyanin at salinities above 30 g/L and 20 g/L,respectively.Culture supernatants of all 5 Pseudomonas isolates possessed the ability to restrict the growth of Vibrio spp.and maximum antagonistic effect on Vibrio harveyi was obtained when they were grown at salinities of 5 to 10 g/L.The marine isolate MCCB117,even when grown at a salinity of 45 g/L possessed the ability to inhibit Vibrio spp.Conclusions:Purification and structural elucidation of antagonistic compound were carried out.ideal for application in freshwater,MCCB102 and MCCB103 in brackish water and MCCB117 and The present investigation showed that Pseudomonas aeruginosa MCCB119 would be MCCB118 in marine aquaculture systems as putative probiotics in the management of vibrios.
文摘The aim of this work was to study the pyocyanin pigment from Pseudomonas aeruginosa DN9.The work involves optimiza-tion of process parameters for enhanced production of pyocyanin pigment under submerged fermentation condition.During optimization process,maximum pyocyanin production(92.12μg/ml)was obtained with carbon source mannitole,nitrogen source peptone,inorganic salt NaCl and metal ion FeSO_(4).Plackett Burman design and Response Surface Methodology(RSM)showed peptone,NaCl and KH_(2)PO_(4)are significant variable in the production of pyocyanin pigment.The FTIR and GC-MS study was done to evaluate structural properties of pyocyanin pigment.The purified pigment was further analyzed as colouring agent and for inhibitory action against pathogenic microorganisms.Thus,present study showed Pseudomonas aeruginosa DN9 as promising culture for pigment production with potential biotechnological application.
