Pyrogallol(pyrogallic acid)is easy to be autooxidized,forming a free radical under alkaline condition,but the mechanism has not been clear.The mechanism of the autooxidation of pyrogallol was studied with ESR,UV and N...Pyrogallol(pyrogallic acid)is easy to be autooxidized,forming a free radical under alkaline condition,but the mechanism has not been clear.The mechanism of the autooxidation of pyrogallol was studied with ESR,UV and NMR techniques and it was found that pyrogallol was autooxidized to a quinone radical under alkaline condition.And at the same time oxygen recieved an electron from the reaction to form superoxide anions,the hydroxyl free radical was then produced by HaberWeiss reaction.The oxygen free radicals generated from this process promoted the autooxidation of pyrogallol.展开更多
Isoproterenol （ISPR） is an important catecholamine‐based drug that is widely used in the treatment of heart disease. However, overdose of this drug is very dangerous to the human body. In this study, a new sensor b...Isoproterenol （ISPR） is an important catecholamine‐based drug that is widely used in the treatment of heart disease. However, overdose of this drug is very dangerous to the human body. In this study, a new sensor based on a pyrogallol red modified‐multiwalled carbon nanotube paste electrode （PGRMMWCNTPE） was prepared and used for high sensitivity determination of ISPR in aqueous solution. Electrocatalytic oxidation of ISPR at the PGRMMWCNTPE was investigated by chronoam‐perometry, cyclic voltammetry, and square‐wave voltammetry. The values of the catalytic rate con‐stant, electron transfer coefficient, and diffusion coefficient for ISPR oxidation were then calculated using voltammetric data. A linear calibration curve was constructed for ISPR concentration in the range 0.8–570μmol/L with a detection limit of 0.47μmol/L ISPR. The sensor was then applied to the determination of ISPR in urine and drug samples with satisfactory results.展开更多
The interactions of pyrogallol red (PR) with proteins and a derivative method for the determination of proteins were proposed. In the pH range 2 56-4 10, PR interacts with proteins, including bovine serum albumin, hu...The interactions of pyrogallol red (PR) with proteins and a derivative method for the determination of proteins were proposed. In the pH range 2 56-4 10, PR interacts with proteins, including bovine serum albumin, human serum albumin, lysozyme, chymotrypsin, resulting in enhanced resonance light scattering(RLS) with the maximal peak at 360 0 nm. With the enhanced RLS, proteins in the range 0-5 0(μg/mL can be determined with the determination limits between 10 3 and 32 2 ng/mL depending on different protein. The effects of foreign substances were tested. The method has been applied to the determination of protein in the synthetic samples and human urine with satisfaction results.展开更多
A novel and facile seed-mediated method for the preparation of monodispersed gold nanorods(GNRs) is presented by introducing pyrogallol as a reductant. Fast Fourier transformation of high-resolution transmission elect...A novel and facile seed-mediated method for the preparation of monodispersed gold nanorods(GNRs) is presented by introducing pyrogallol as a reductant. Fast Fourier transformation of high-resolution transmission electron microscopy reveals that the synthesized GNRs are single crystalline. The longitudinal surface plasmon resonance of GNRs can be finely tuned by varying silver ion concentrations or seed amounts. Also, both thick(diameter >30 nm) and thin(diameter <10 nm) GNRs with exceptional monodispersity can be well prepared by this method. These findings indicate that this method has a greater performance in controlling the morphology of GNRs than that of traditional approach with ascorbic acid as a reductant.展开更多
Two novel 3D metal-organic frameworks(MOFs)with cds network,{[Me NH_3]_7[Ln_8(Pg C_2)_2(H_2O)_y(HCOO)_7]}_n·x(Solvent)(FJI-Y4,FJI=Fujian Institute,Ln=Gd,y=12;FJI-Y5,Ln=Dy,y=11;Pg C_2=C-ethylpyrogallol[4]arene),ba...Two novel 3D metal-organic frameworks(MOFs)with cds network,{[Me NH_3]_7[Ln_8(Pg C_2)_2(H_2O)_y(HCOO)_7]}_n·x(Solvent)(FJI-Y4,FJI=Fujian Institute,Ln=Gd,y=12;FJI-Y5,Ln=Dy,y=11;Pg C_2=C-ethylpyrogallol[4]arene),based on unprecedented dimeric pyrogallol[4]arene-based Ln_8metal-organic nanocapsule(MONC)supramolecular building blocks and formate linkers,have been prepared under solvothermal conditions.To our best of knowledge,they present not only the first two examples of 3D hierarchical structures constructed from MONCs in metal-pyrogallol[4]arene system,but also the first two examples of MOFs based on lanthanide MONCs.Remarkably,the inner cavity volume of the Ln_8capsule in FJI-Y4 and FJI-Y5 is approximately151?~3,which is larger than those found in previous transition metal-seamed dimeric Pg C_n-based MONCs.Magnetic investigation on FJI-Y4 suggests a significant magnetocaloric effect(23.97 J kg^(-1)K^(-1),ΔH=7 T,2.5 K),while FJI-Y5 exhibits slow relaxation of the magnetization.展开更多
The controlled self-assembly of discrete metal-organic nanocapsules(MONCs),and metal-organic frameworks(MOFs)based on the MONCs are achieved.Specifically,the solvothermal reaction of nickel nitrate hexahydrate and C-m...The controlled self-assembly of discrete metal-organic nanocapsules(MONCs),and metal-organic frameworks(MOFs)based on the MONCs are achieved.Specifically,the solvothermal reaction of nickel nitrate hexahydrate and C-methylpyrogallol[4]arene in mixed DMF/MeOH solution leads to the unexpected form of discrete nickel-seamed hexameric pyrogallol[4]arene MONCs,and MONC-based three-dimensional(3D)MOF.Notably,the latter MOF is constructed from the aforementioned nickelseamed MONC building blocks and formate linkers in-situ generated from the hydrolysis of DMF solve nt.Interestingly,introducing pyridine and formic acid in the reaction conditions leads to the controlled assemblies of the discrete MONC and MONC-based 3D MOF structures.Moreover,the variabletemperature magnetic susceptibilities of both the abovementioned compounds have been investigated,indicating typical antiferromagnetic interactions between the metal centers.展开更多
The roasting process of pyrogallol,a polyphenol compound distributed in coffee beverages,significantly enhanced itsα-glucosidase inhibitory activity.In this study,a bioassay-guided isolation of the thermal reaction p...The roasting process of pyrogallol,a polyphenol compound distributed in coffee beverages,significantly enhanced itsα-glucosidase inhibitory activity.In this study,a bioassay-guided isolation of the thermal reaction products of pyrogallol led to the identification of two potentα-glucosidase inhibitors,4-4′dimer of pyrogallol(4,4′-DP)and 4-5′dimer of pyrogallol(4,5′-DP).Theirα-glucosidase inhibitory activity was higher than that of pyrogallol,as evidenced by comparing the IC_(50)values(206.2±1.2μM for 4,4′-DP,187.6±2.6μM for 4,5′-DP,2660±60.1μM for pyrogallol).And the roasting products were more potentα-glucosidase inhibitors compared to acarbose(IC_(50)=695±12.7μM).Enzyme kinetics demonstrated that 4,4′-DP and 4,5′-DP inhibitedα-glucosidase in an uncompetitive and a non-competitive manner,respectively.Docking simulations revealed that the main interaction forces between these two compounds andα-glucosidase were hydrogen bonding and hydrophobic effect.These results suggested that a simple roasting process might increase theα-glucosidase inhibitory activity of pyrogallol-containing foods such as coffee beverages.展开更多
The content of flavones of the seabuckthorn ( Hippophae rhamnoides L. )root bark,branch bark and leaves were determined using UV vis spectrophotometry.In the meantime,the scavenging hydroxyl free radical(OH ·)and...The content of flavones of the seabuckthorn ( Hippophae rhamnoides L. )root bark,branch bark and leaves were determined using UV vis spectrophotometry.In the meantime,the scavenging hydroxyl free radical(OH ·)and superoxide anion free radical(O 2 ·- )of the extracts of seabuckthorn root bark,branch bark and leaves were measured through Fenton reaction and pyrogallol autoxidation method.The results showed that the seabuckthorn root bark,branch bark and leaves had a strong capacity on scavenging free radical.The scavenging rates of hydroxyl free radical were 69.2%,62.2% and 74.1%,respectively.The inhibition rates of superoxide anion free radical were 10.5%,12.3% and 33.3%,respectively.Finally the antioxidant mechanism of seabuckthorn flavones were tentatively explored.展开更多
The ability of water soluble β alanine C 60 adducts for superoxide anion radical scavenging has been studied by spectrophotometry and the modified pyrogallol auto oxidation method. It is found that β alanine C 60 ad...The ability of water soluble β alanine C 60 adducts for superoxide anion radical scavenging has been studied by spectrophotometry and the modified pyrogallol auto oxidation method. It is found that β alanine C 60 adducts have an excellent efficiency in eliminating superoxide radical generated from auto oxidation of pyrogallol. At a mass concentration level of 150 μg/mL for β alanine C 60 adducts, the radical scavenging efficiency is approximately equivalent to one enzyme unit, in vitro experiments show that β alanine C 60 adducts can enhance the activity of mouse thymus cells and prolong their life time span. It reveals the potential use of these β alanine C 60 adducts as the novel potent free radical scavengers in biological systems.展开更多
文摘Pyrogallol(pyrogallic acid)is easy to be autooxidized,forming a free radical under alkaline condition,but the mechanism has not been clear.The mechanism of the autooxidation of pyrogallol was studied with ESR,UV and NMR techniques and it was found that pyrogallol was autooxidized to a quinone radical under alkaline condition.And at the same time oxygen recieved an electron from the reaction to form superoxide anions,the hydroxyl free radical was then produced by HaberWeiss reaction.The oxygen free radicals generated from this process promoted the autooxidation of pyrogallol.
基金Majlesi Branch, Islamic Azad University, for their support
文摘Isoproterenol （ISPR） is an important catecholamine‐based drug that is widely used in the treatment of heart disease. However, overdose of this drug is very dangerous to the human body. In this study, a new sensor based on a pyrogallol red modified‐multiwalled carbon nanotube paste electrode （PGRMMWCNTPE） was prepared and used for high sensitivity determination of ISPR in aqueous solution. Electrocatalytic oxidation of ISPR at the PGRMMWCNTPE was investigated by chronoam‐perometry, cyclic voltammetry, and square‐wave voltammetry. The values of the catalytic rate con‐stant, electron transfer coefficient, and diffusion coefficient for ISPR oxidation were then calculated using voltammetric data. A linear calibration curve was constructed for ISPR concentration in the range 0.8–570μmol/L with a detection limit of 0.47μmol/L ISPR. The sensor was then applied to the determination of ISPR in urine and drug samples with satisfactory results.
文摘The interactions of pyrogallol red (PR) with proteins and a derivative method for the determination of proteins were proposed. In the pH range 2 56-4 10, PR interacts with proteins, including bovine serum albumin, human serum albumin, lysozyme, chymotrypsin, resulting in enhanced resonance light scattering(RLS) with the maximal peak at 360 0 nm. With the enhanced RLS, proteins in the range 0-5 0(μg/mL can be determined with the determination limits between 10 3 and 32 2 ng/mL depending on different protein. The effects of foreign substances were tested. The method has been applied to the determination of protein in the synthetic samples and human urine with satisfaction results.
基金supported by the National Natural Science Foundation of China(61271056,61301039,61102031,21205036)Hunan Provincial Natural Science Foundation of China(13JJ4091,12JJ6060)+2 种基金China Postdoctoral Science Foundation funded projects(2014M550261,2014T70459)the Scientific Research Fund of Hunan Provincial Education Department(13A003)the Economical Forest Cultivation and Utilization of 2011 Collaborative Innovation Center in Hunan Province[(2013)448]
文摘A novel and facile seed-mediated method for the preparation of monodispersed gold nanorods(GNRs) is presented by introducing pyrogallol as a reductant. Fast Fourier transformation of high-resolution transmission electron microscopy reveals that the synthesized GNRs are single crystalline. The longitudinal surface plasmon resonance of GNRs can be finely tuned by varying silver ion concentrations or seed amounts. Also, both thick(diameter >30 nm) and thin(diameter <10 nm) GNRs with exceptional monodispersity can be well prepared by this method. These findings indicate that this method has a greater performance in controlling the morphology of GNRs than that of traditional approach with ascorbic acid as a reductant.
基金supported by the Strategic Priority Research Program of the Chinese Academy of Sciences (XDB20000000)the National Natural Science Foundation of China (21390392, 51603206)the Nature Science Foundation of Fujian Province (2016J05056)
文摘Two novel 3D metal-organic frameworks(MOFs)with cds network,{[Me NH_3]_7[Ln_8(Pg C_2)_2(H_2O)_y(HCOO)_7]}_n·x(Solvent)(FJI-Y4,FJI=Fujian Institute,Ln=Gd,y=12;FJI-Y5,Ln=Dy,y=11;Pg C_2=C-ethylpyrogallol[4]arene),based on unprecedented dimeric pyrogallol[4]arene-based Ln_8metal-organic nanocapsule(MONC)supramolecular building blocks and formate linkers,have been prepared under solvothermal conditions.To our best of knowledge,they present not only the first two examples of 3D hierarchical structures constructed from MONCs in metal-pyrogallol[4]arene system,but also the first two examples of MOFs based on lanthanide MONCs.Remarkably,the inner cavity volume of the Ln_8capsule in FJI-Y4 and FJI-Y5 is approximately151?~3,which is larger than those found in previous transition metal-seamed dimeric Pg C_n-based MONCs.Magnetic investigation on FJI-Y4 suggests a significant magnetocaloric effect(23.97 J kg^(-1)K^(-1),ΔH=7 T,2.5 K),while FJI-Y5 exhibits slow relaxation of the magnetization.
基金the Strategic Priority Research Program of the Chinese Academy of Sciences(No.XDB20000000)the National Nature Science Foundation of China(No.51603206)the Nature Science Foundation of Fujian Province(No.2016J05056)。
文摘The controlled self-assembly of discrete metal-organic nanocapsules(MONCs),and metal-organic frameworks(MOFs)based on the MONCs are achieved.Specifically,the solvothermal reaction of nickel nitrate hexahydrate and C-methylpyrogallol[4]arene in mixed DMF/MeOH solution leads to the unexpected form of discrete nickel-seamed hexameric pyrogallol[4]arene MONCs,and MONC-based three-dimensional(3D)MOF.Notably,the latter MOF is constructed from the aforementioned nickelseamed MONC building blocks and formate linkers in-situ generated from the hydrolysis of DMF solve nt.Interestingly,introducing pyridine and formic acid in the reaction conditions leads to the controlled assemblies of the discrete MONC and MONC-based 3D MOF structures.Moreover,the variabletemperature magnetic susceptibilities of both the abovementioned compounds have been investigated,indicating typical antiferromagnetic interactions between the metal centers.
基金This work was supported by the National Natural Science Foundation of China[grant number 82003650]TCM Science and Technology Project of Shandong Province,China[grant number 2020Q050].
文摘The roasting process of pyrogallol,a polyphenol compound distributed in coffee beverages,significantly enhanced itsα-glucosidase inhibitory activity.In this study,a bioassay-guided isolation of the thermal reaction products of pyrogallol led to the identification of two potentα-glucosidase inhibitors,4-4′dimer of pyrogallol(4,4′-DP)and 4-5′dimer of pyrogallol(4,5′-DP).Theirα-glucosidase inhibitory activity was higher than that of pyrogallol,as evidenced by comparing the IC_(50)values(206.2±1.2μM for 4,4′-DP,187.6±2.6μM for 4,5′-DP,2660±60.1μM for pyrogallol).And the roasting products were more potentα-glucosidase inhibitors compared to acarbose(IC_(50)=695±12.7μM).Enzyme kinetics demonstrated that 4,4′-DP and 4,5′-DP inhibitedα-glucosidase in an uncompetitive and a non-competitive manner,respectively.Docking simulations revealed that the main interaction forces between these two compounds andα-glucosidase were hydrogen bonding and hydrophobic effect.These results suggested that a simple roasting process might increase theα-glucosidase inhibitory activity of pyrogallol-containing foods such as coffee beverages.
文摘The content of flavones of the seabuckthorn ( Hippophae rhamnoides L. )root bark,branch bark and leaves were determined using UV vis spectrophotometry.In the meantime,the scavenging hydroxyl free radical(OH ·)and superoxide anion free radical(O 2 ·- )of the extracts of seabuckthorn root bark,branch bark and leaves were measured through Fenton reaction and pyrogallol autoxidation method.The results showed that the seabuckthorn root bark,branch bark and leaves had a strong capacity on scavenging free radical.The scavenging rates of hydroxyl free radical were 69.2%,62.2% and 74.1%,respectively.The inhibition rates of superoxide anion free radical were 10.5%,12.3% and 33.3%,respectively.Finally the antioxidant mechanism of seabuckthorn flavones were tentatively explored.
文摘The ability of water soluble β alanine C 60 adducts for superoxide anion radical scavenging has been studied by spectrophotometry and the modified pyrogallol auto oxidation method. It is found that β alanine C 60 adducts have an excellent efficiency in eliminating superoxide radical generated from auto oxidation of pyrogallol. At a mass concentration level of 150 μg/mL for β alanine C 60 adducts, the radical scavenging efficiency is approximately equivalent to one enzyme unit, in vitro experiments show that β alanine C 60 adducts can enhance the activity of mouse thymus cells and prolong their life time span. It reveals the potential use of these β alanine C 60 adducts as the novel potent free radical scavengers in biological systems.
