前言

前言中国毒理学会工业毒理学专业委员会在中国毒理学会大力支持下，于１９９４年１１月２９日至１２月１日在广州隆重召开成立大会并举行第一次学术交流会。卫生部卫生监督司向大会发来贺信，向工业毒理学专业委员会的成立表示热烈的祝贺。贺信中说：工业毒理学专业委员会...

TNT诱导动、植物微核效应的观察

ＴＮＴ诱导动、植物微核效应的观察戴敏，李斌（西安兵器工业卫生研究所，西安７１００６１）作者应用蚕豆根尖细胞微核和小鼠骨髓微核测试法，观察ＴＮＴ对动物及植物的诱变效应。结果表明：不同剂量的ＴＮＴ浸泡蚕豆根尖染毒３．５ｈ后，各剂量组（０．５、５、５０、１...

Once upon a time biomass burning in the western Alps: Nesting effects of climate and local drivers on long-term subalpine fires

Background:The present article questions the relative importance of local-and large-scale processes on the long-term dynamics of fire in the subalpine belt in the western Alps.The study is based on soil charcoal dating and identification,several study sites in contrasting environmental conditions,and sampling of soil charcoal along the elevation gradient of each site.Based on local differences in biomass combustion,we hypothesize that local-scale or landscape-scale processes have driven the fire history,while combustion homogeneity supports the hypothesis of the importance of large-scale or macro-ecological processes,especially climate.Results:Biomass burning during the Holocene resulted from the nesting effects of climate,land use,and altitude,but was little influenced by slope exposure(north versus south),soil(dryness,pH,depth),and vegetation.The mid-Holocene(6500–2700 cal BP)was an important period for climate-driven biomass burning in the subalpine ecosystems of the western Alps,while fires over the last 2500 years appear much more episodic,prompting us to speculate that human activity has played a vital role in their occurrence.Conclusion:Our working hypothesis that the strength of local drivers should offset the effects of regional climate is not validated.The homogeneity of the fire regime between sites thus underscores that climate was the main driver during the Holocene of the western Alps.Long-term subalpine fires are controlled by climate at the millennial scale.Local conditions matter for little in determining variability at the century scale.The mid-Holocene was a chief period for climatic biomass burning in the subalpine zone,while fires during the late Holocene appear much more episodic,suggesting that social drivers has exercised key function on their control.

LAL test and RPT for endotoxin detection of CPT-11/DSPE-mPEG_(2000) nanoformulation: What if traditional methods are not applicable?

Endotoxin detection is an important step in drug characterization. Herein we found that a chemotherapeutic drug nanoformulation composed of irinotecan hydrochloride(CPT-11) and an amphiphilic molecule DSPE-mPEG_(2000) can interfere with the limulus amebocyte lysate assay(LAL). Furthermore, the rabbit pyrogen test(RPT) results indicated that at a relatively high dosage, the drug irinotecan hydrochloride can induce a hypothermia effect which may render the RPT results ambiguous in determination of the safety of the drug formulation.Our findings demonstrate limitations of endotoxin detection in micellar drugs,and call for the necessity of developing reliable endotoxin detection methods that can overcome the interference of nanomaterials in order to better ensure the drug safety of patients in future pharmaceutical drug development.

Chemical characterization of smoke from the production process of wood-plastic composites

The chemical composition of unpleasant smell, emitted from the production process of wood-plastic composites using Manchurian ash sawdust （Fraxinus mandschurica Rupr.） and polypropylene powder as the raw material, was investigated. Wood sawdust and polypropylene powder were subjected to heat treatment to 290℃ during 8 min （the conditions were similar to those employed on an industrial scale）. The emitted compounds were collected and analyzed by gas chromatography-mass spectrometry （GC-MS）. The analytical results showed that the unpleasant smell was emitted from the pyrogenation of wood sawdust rather than from the polypropylene powder. Nine types of compounds （hydrocarbons, ethers, phenols, aldehydes, ketones, alcohols, acids and their derivatives, furan and its derivatives, and nitrogen-containing compounds） were collected in the gas phase during heating. Among those 126 components detected by GC-MS, 112 compounds were identified.

Streptococcal Pyrogenic Exotoxin Genes SpeA and SpeB in Isolates of Streptococcus pyogenes from Children with Pharyngitis, Gezira State, Sudan

Background: Streptococcus pyogenes (group A streptococcus, GAS) is an important human bacterial pathogen. This organism possesses many virulence factors, Streptococcal pyrogenic exotoxinone of these. Aim: Detection of Streptococcal pyrogenic exotoxin SpeA and SpeB in isolated Streptococcus pyogenes. Methods: Tow hundred throat swab samples were collected from children with pharyngitis referred to Pediatric Teaching hospital and ENT hospital Wad medani, Sudan, from January to November 2021. The questionnaire was filled out to collect clinical and demographic data. Throat swabs were collected and processed with the standard microbiological procedure to isolate Streptococcus pyogenes. Antimicrobial susceptibility testing was done on all GAS isolates using the Kirby Bauer disk diffusion method according to clinical laboratory standard institute (CLSI) guidelines. Detection of Spy 1258 gene and Streptococcal pyrogenic exotoxins SpeA and SpeB were done by using Multiplex PCR. Results: Amongst the Tow hundred collected samples fifty-one isolates (25.5%) were identified as S. pyogenes. Antibiotic susceptibility testing showed that all the GAS isolates were sensitive to Azithromycin and Penicillin. Sensitivity to Erythromycin, Gentamicin, Clarithromycin, Amoxicillin and Cephalexin were 88.2%, 86.3%, 45.1%, 41.2%, 13.7%, respectively. SpeA was detected in 17 (33.3%) and SpeB in 48 (94.1%). Conclusion: Streptococcal pyrogenic exotoxin genes SpeA and SpeB were detected in 17 (33.3%) and 48 (94.1%) respectively of Streptococcus pyogenes isolates.

Environmental Impact Assessment of the Application of Pyrogenic Carbon in Soil

World increasing population and use of energy for transportation and electricity are demanding more extensive and more efficient use of land for agriculture;aiming to both food and biofuel supplies. This communication assesses the possible improvements in soil fertility, capture of greenhouse gas, and rainfall, as a result of the large scale terrestrial application of pyrogenic carbon aiming for desert greening. Fossil hydrocarbon coke is taken into account for this proposal because of the exhaustion of light petroleum proven reserves that is leading to a scenario of abundant coke production from the processing of non-conventional reserves.

Assessment of Standard Criteria Implementation in Heamodialysis Water Treatment Units in Sharkia Governorate

Introduction: Haemodialysis is the most well-established form of treatment for ESRD. Method: To evaluate the implementation of standard criteria in heamodialysis water treatment units in Sharkia governorate and to determine the weak points in application of standard criteria, and reach the optimal standards to improve pt. outcomes, across the sectional study was conducted at 30 heamodialysis units of Sharkia governorate, using a modified questionnaire was developed based on MOH protocol and international guidelines such as CARI guidelines, AAMI guidelines and others by the researchers. All data were collected, tabulated and statistically analyzed using SPSS 22.0 for windows (SPSS Inc., Chicago, IL, USA). Results: Of the 30 units, the majority more than 80% of the units achieved the infrastructure and schematic structure, contain water purification devices, good infection control policies, proper chemical disinfection, good monitoring and quality control, accepted maintenance technician evaluation and collected processed water samples results matched decree of 63 for 1996. Conclusion: Most of the studied units nearly fulfilled the standard specifications of both MOH and AAMI. Ensuring that water quality meets AAMI standards and recommendations will minimize patient exposure to potential contaminants such as chemical hazards and endotoxemia associated with the use of the treated water for HD.

Nutrients Available and Stable Carbon from Archaeological Black Earth

The present study aims affinity between available concentrations of phosphorus, micronutrients (Cu, Zn and Mn) and stable carbon (SC) in archaeological black earth (ABE) from area “Ilha de Terra”/Caxiuanã—Pará. The relevance of this study refers especially to the determination of carbon concentrations (total, oxidable and semi-labile carbon) directly associated with those of pyrogenic carbon, common in ABE, which should add their contributions to the understanding of soil organic matter recalcitrance. Chemical properties such as CEC and base saturation were determined for ABE and subjacent Latossoil. The available concentrations of nutrients were performed from sequential extraction F1 to F5 phases. Phosphorus was obtained by spectrophotometric method and micronutrients by MP-AES. TOC was determined by the combustion method;stable carbon was obtained from thermoxidation method CTO-375. The following results were obtained from ABE: CEC effective = 21 to 28 (cmolc·L-1);base saturation = 58% to 69%;Carbon concentrations (%): TOC = 2.95 to 3.94;SC = 0.25 to 0.88;semi-labile carbon = 1.75 to 3.63;oxidable = 2.11 to 3.65;inorganic carbon 0.01 to 0.38;P2O5 concentrations (mg·kg-1) in the ABE phases F3 (Fe-Mn oxides, F4 (organic ) and F5 (residual)) in the following order: 35 to 65;200 to 400;140 to 230. It was concluded that the chemical properties in ABE from Ilha de Terra site fall within the range of fertile and the high concentrations of phosphorus are biogenic origin. Phases F3 and F4 are those considered nutrient stocks from ABE studied area.

A novel alternative for pyrogen detection based on a transgenic cell line

Pyrogen,often as a contaminant,is a key indicator affecting the safety of almost all parenteral drugs(including biologicals,chemicals,traditional Chinese medicines and medical devices).It has become a goal to completely replace the in vivo rabbit pyrogen test by using the in vitro pyrogen test based on the promoted‘reduction,replacement and refinement’principle,which has been highly considered by regulatory agencies from different countries.We used NF-κB,a central signalling molecule mediating inflammatory responses,as a pyrogenic marker and the monocyte line THP-1 transfected with a luciferase reporter gene regulated by NF-κB as an in vitro model to detect pyrogens by measuring the intensity of a fluorescence signal.Here,we show that this test can quantitatively and sensitively detect endotoxin(lipopolysaccharide from different strains)and nonendotoxin(lipoteichoic acid,zymosan,peptidoglycan,lectin and glucan),has good stability in terms of NF-κB activity and cell phenotypes at 39 cell passages and can be applied to detect pyrogens in biologicals(group A&C meningococcal polysaccharide vaccine;basiliximab;rabies vaccine(Vero cells)for human use,freeze-dried;Japanese encephalitis vaccine(Vero cells),inactivated;insulin aspart injection;human albumin;recombinant human erythropoietin injection(CHO Cell)).The within-laboratory reproducibility of the test in three independent laboratories was 85%,80%and 80%and the interlaboratory reproducibility among laboratories was 83.3%,95.6%and 86.7%.The sensitivity(true positive rate)and specificity(true negative rate)of the test were 89.9%and 90.9%,respectively.In summary,the test provides a novel alternative for pyrogen detection.

THE EFFECTS OF PERFUSION OF LATERAL VENTRICLE WITH CaCl_2 ON THE FEBRILE RESPONSE AND cAMP CONTENT IN PLASMA AND CEREBROSPINAL FLUID DURING LP-INDUCED FEVER

Artificial cerebrospinal fluid (c.s.f.) containing 40 mmol/L excess calcium was perfus-ed through the lateral ventricles of New Zealand white rabbits in order to reduce the Na^+/Ca^(++) ratio in the brain and the effects on both the febrile response and adenosine cyclic mo-nophosphate (cAMP) concentration in plasma and c.s.f, during leucocytic pyrogen (LP)-induced fever were observed. The results showed that cAMP concentration in c.s.f, increas-ed significantly during LP-induced fever while the cAMP level in Plasma remained unchang-ed, and the perfusion of artificial c.s,f, containing 40 mmol/L excess calcium can signif-icantly inhibit not only the febrile response but also the increase in c.s.f, cAMP level,while there appears no effect on plasma cAMP concentration, thus demonstrating that theincrease of Na^+/Ca^(++) ratio causing the increase of cAMP content in the brain may be anessential link in the pathogenesis of LP-induced fever.

Calciomics:prediction and analysis of EF-hand calcium binding proteins by protein engineering

Ca2+ plays a pivotal role in the physiology and biochemistry of prokaryotic and mammalian organisms.Viruses also utilize the universal Ca2+ signal to create a specific cellular environment to achieve coexistence with the host,and to propagate.In this paper we first describe our development of a grafting approach to understand site-specific Ca2+ binding properties of EF-hand proteins with a helix-loop-helix Ca2+ binding motif,then summarize our prediction and identification of EF-hand Ca2+ binding sites on a genome-wide scale in bacteria and virus,and next report the application of the grafting approach to probe the metal binding capability of predicted EF-hand motifs within the streptococcal hemoprotein receptor(Shr) of Streptococcus pyrogenes and the nonstructural protein 1(nsP1) of Sindbis virus.When methods such as the grafting approach are developed in conjunction with prediction algorithms we are better able to probe continuous Ca2+-binding sites that have been previously underrepresented due to the limitation of conventional methodology.

Biochar Increases Diuron Sorption and Reduces the Potential Contamination of Subsurface Water with Diuron in a Sandy Soil

The herbicide diuron is widely used in agricultural areas in Brazil, whereas it has high potential for subsurface water contamination due to its physicochemical characteristics. Recent studies have demonstrated the potential of biochar as a sorbent and possible pesticide leaching mitigation. The objective of this study was to investigate the long-term effect of biochar application on the kinetics of sorption and desorption of diuron in a Cerrado Haplic Plinthosol. Samples were collected in an experiment conducted in the field in a randomized block design consisting of the combination of two levels of fertilizer application (0 and 300 kg ha −1 of 5-25-15 formula of NPK fertilizers) and three doses of biochar (0, 16, and 32 Mg ha −1 ). The Freundlich isotherm accurately described the sorption of diuron in all treatments. Biochar application increased the sorption and reduced the desorption of diuron. This effect was attributed to the contribution of biochar to total organic carbon (C) and C in the humin fraction and to the increase in the reactivity of the humic acid and humin fractions, which was significantly highly correlated with the sorption coefficient ( K f ). A positive correlation between the partition coefficient of organic C and K f confirmed the importance of the soil organic compartment for the sorption of diuron. The higher diuron sorption and lower diuron desorption capacities of sandy soils after biochar application could reduce the potential risk of diuron leaching and contamination of subsurface water.

Sources and environmental fate of pyrogenic polycyclic aromatic hydrocarbons(PAHs)in the Arctic

Polycyclic aromatic hydrocarbons(PAHs)are large class of hydrophobic,semi-volatile organic contaminants that may enter the environment from both natural sources and anthropogenic activities.Pyrogenic PAHs arise from the incomplete combustion of fossil fuels and organic matter and following dispersal via long-range transport and may subsequently deposit in surface waters,soils and sediments of remote regions,including the Arctic.The current review summarizes and discusses Arctic data that is available for combustion-derived PAHs between 2004 and early 2018,focusing largely on data collected from remote,unexploited Arctic regions and from studies that provide some evidence of a pyrogenic origin.The increasing use of attribution ratios,which aid in discriminating PAHs from petrogenic or pyrogenic sources,suggest PAHs found in Arctic marine waters and sediment predominantly originate from natural underwater seeps,while those measured in air,freshwater,and terrestrial environments are likely to have originated from atmospheric and combustion-derived sources.Modeling efforts indicate that atmospheric PAHs in the Canadian and Norwegian Arctic are likely to have originated in the northern hemisphere e predominantly from Western Russia,northern Europe,and North America.East Asia appears to be a minor source of PAHs to the Arctic,despite contributing more than 50%of global PAH emissions.In comparison to the growing data for atmospheric PAHs,environmental data for these compounds in terrestrial and freshwater environments remain scarce.PAHs have been detected in Arctic biota from terrestrial,freshwater and marine environments,indicating exposure,however,levels are generally low,as most organisms efficiently metabolize parent PAHs.Globally,PAH emissions are expected to decline in the future,however models suggest the Arctic may not experience the same magnitude of decline projected for other world regions.Furthermore,future changes in climate may contribute to a re-volatilization of environmental PAHs,providing a source of secondary emissions to the Arctic atmosphere,emphasizing the importance of future monitoring for understanding the sources,fate and impacts of PAHs in the Arctic.

A nanoscale observation to explain the discrepancy of electron exchange capacities between biochar containing comparable surface redox-active moieties

Biochar,possessing electron exchange capacities(EEC),is generally involved in environmental redox reactions due to the presence of redox-active moieties(RAMs).The phenomenon that chars containing comparable RAMs possess differential EEC revealed that the accessibility of RAMs is important to the redox properties.However,many studies have focused on the type of RAMs,whereas the distribution has been insufficiently investigated.Herein,we achieved nanoscale observation of electroactive moieties on the surface of six chars using a conductive atomic force microscope.For the two specific kinds of chars with submicron particles and opposite current distributions,the submicron particles took up only 1-4‰wt of biochar accounting for approximately 30-50%of electron-donating capacity(EDC),and electron-accepting capacity(EAC)became 87%and 1.40 times as before after removing submicron particles,respectively.Meanwhile,the combined impact of RAMs and surface topography(that uneven distribution of RAMs resulted in outstanding EEC by enhancing accessibility)was clarified.Furthermore,direct evidence of the link between char structure and EEC(that condensed aromatic structures were indispensable to EAC while both heteroatoms and amorphous aromatics contributed to EDC)was established.These findings can aid in understanding the functions of biochar in biotic and abiotic redox processes.

Synthesis and luminescence properties of Mn^(3+),Bi^(3+) co-doped Y_6W0_(12) for blue phosphor

Mn^3＋ and Bi^3＋ co-doped Y6WO12 samples with hexagonal structure were synthesized via an improved salt pyrogenation method at a temperature region of 700-1100 ℃ for 3 h. In Y6WO12, Mn^3＋, substituting y^3＋, occupies a seven-coordination site and its energy levels are treated in near Oh symmetry. The samples doped by Mn^3＋ alone emit the most intensive blue light at 420 nm under excitation at 247 nm due to charge transition （CT）. The mechanism of sensitization of Bi^3＋ for Y6WO12：Mn^3＋ was also analyzed by taking account of metal-to-metal chargetransfer （MMCT） from Bi^3＋ to Mn^3＋. As a consequence, the phosphor Y6WO12：Mn^3＋/Bi^3＋ can emit blue light under radiation of 370 nm, and the emission intensity is enhanced about five times by the sensitizer Bi^3＋. The optimal doping concentration of Bi^3＋ is determined as 1 at% for the emission at 420 nm in Y6WO12：0.5 at% Mn^3＋ phosphors.

Microwave-assisted combustion to produce benzene polycarboxylic acids as molecular markers for biochar identification and quantification

Biochar is a promising carbon dioxide removal(CDR)technology for climate change mitigation.Current procedures for its determination are lengthy,labor-intensive,and difficult to conduct.Benzene polycarboxylic acids(BPCA)are the most promising molecular markers for identification and quantification of biochar and its quality as they specifically represent the stable polyaromatic backbone of biochar.Therefore,using the BPCA method,its stability and,thus,its C sequestration potential could be used for CDR accounting.The current BPCA method relies on a specific high-pressure digestion apparatus,which is not available around the world.Therefore,the aims of the present work were(i)to compare the conventional high-pressure nitric acid oxidation with a microwave-assisted digestion technique and optimize the oxidation conditions in such a way that previous results are comparable with future ones,and(ii)to significantly reduce the digestion time of soil samples of 8 h and to develop a suitable routine method that produces comparable and reproducible results.For this purpose,soil and control sample series were prepared for different temperature-time-program.Obtained results were compared with the values of the conventional method both for individual samples and for the whole dataset separately.To ensure the representative-ness of the results,in addition to various soil samples with different properties,we included two reference materials into our data set,one without biochar(wheat flour)and a biochar sample.Our results showed that conventional nitric acid oxidation in the BPCA determination at 170°C and 8 h can be substituted by digestion in a microwave reaction system(CEM Mars6)at 190°C and 1 h.Our results further showed that this condition needs to be strictly matched,because,otherwise,over-or underestimation of biochar quantity and/or quality will be the consequence.The goal of a less time-consuming BPCA extrac-tion from soil samples was achieved by reducing the extraction time from 8 to 1 h using the microwave-assisted method.However,one disadvantage of the new method is that five times more sample material and chemicals are needed for further BPCA analysis,compared to the original method.

Actions of four organic acids in radix isatidis on endotoxin-neutralization investigated by kinetic turbidimetric assay

OBJECTIVE:To investigate anti-endotoxin action of four OAs reacted with endotoxin by the LAL assay with KTA.METHODS:Using a incubating kinetic tube reader and kinetic turbidimetric assay(KTA),the concentration-response time curve of endotoxin reacted with limulus amebocyte lysate(LAL) at 37℃ were obtained and the action of four organic acids(OAs) on it were investigated.The four OAs were benzoic acid,salicylic acid,syringic acid and 2-amino-benzoic acid from Radix isatidis.Meanwhile,the temperature variation caused by endotoxin with the four OAs was studied by the rabbit pyrogen test(RPT).RESULTS:It was showed that a low concentration(1 mg/mL) of the four OAs had a little effect of anti-endotoxin,and when the concentrations of the four OAs were 30 mg/mL,the endotoxin was neutralized completely.The relationships between the concentrations of endotoxin and the OAs were all linear with correlation coefficients of greater than 0.9995,indicating that the four OAs all had strong anti-endotoxin action,while syringic acid had the strongest action among the four OAs with IC50 of 12.84 mg/mL.CONCLUSION:The investigations of KTA agreed well with the results obtained by means of RPT.

Investigation of a novel pyrogenic pulser in a laboratory motor

In this paper,a novel pyrogenic pulser was designed both analytically and numerically and was evaluated with empirical tests.The motivation of this study was the need for active control of the aero acoustic pressure oscillations by injecting the secondary flow into the solid rocket motor.First,in brief,pyrotechnic and pyrogenic pulsers were introduced,and then analytical governing equations were presented in three transient,sinusoidal and Hercules methods.In order to understand the internal pressure of the pulsar and its plume length,the injection flow field was evaluated using the ANSYS-Fluent software with both k-ωSST and k-εRealizable models both at ambient and motor pressure.After that,the design and manufacturing of the pulser hardware and the test process were described.Finally,analytical,numerical and experimental results were discussed.The results show that there is a good correlation between the transient analysis in theory and the numerical solution by the k-ωSST model and the empirical test data.In addition,pyrogenic pulsers design depends on various parameters of motor and pulser charge performance prediction.The quality of pulser charge bonding to its insulator and erosion of its throat path due to injection have an important role to obtain a desirable pulser mass flow rate and plume length.

EFFECTS OF SODIUM SALICYLATE ON THE FEBRILE RESPONSE AND INCREASED LEVELS OF CYCLIC AMP IN CEREBRO-SPINAL FLUID DURING ENDOGENOUS PYROGEN-INDUCED FEVER IN RABBITS

To further evaluate the causality between endogenous pyrogen (EP)-induced fever andcyclic adenosine- 3’, 5’- monophosphate (cyclic AMP) level. the effects of sodium salicylate(SS) on the febrile response and increased levels of cyclic AMP in both cerebrospinal fluid(c.s.f.) and plasma during EP- induced fever in rabbits were observed. The results suggestthat cyclic AMP is probably involved in the central mediation of EP-induced fever and thatincreased concentration of cyclic AMP in c.s.f. associated with EP- induced fever is not theresult of temperature elevation but appears to be caused by the increased synthesis in the cen-tral nervous system. In addition it is confirmed that blood is impossibly a contributorysource of increased cyclic AMP in c.s.f. during EP fever, and that SS may act subsequentto the increase in cyclic AMP.