Bacterial wilt caused by the soil-borne plant pathogen Ralstonia solanacearum is a devastating disease worldwide.Upon plant colonization,R.solanacearum replicates massively,causing plant wilting and death;collapsed in...Bacterial wilt caused by the soil-borne plant pathogen Ralstonia solanacearum is a devastating disease worldwide.Upon plant colonization,R.solanacearum replicates massively,causing plant wilting and death;collapsed infected tissues then serve as a source of inoculum.In this work,we show that the plant metabolic pathway mediated by pyruvate decarboxylases(PDCs)contributes to plant tolerance to bacterial wilt disease.Arabidopsis and tomato plants resp ond to R.solanacearum infection by in creasing PDC activity,and plants with deficient PDC activity are more susceptible to bacterial wilt.Treatment with either pyruvic acid or acetic acid(substrate and product of the PDC pathway,respectively)enhances plant tolerance to bacterial wilt disease.An effector protein secreted by R.solanacearum,RipAK,interacts with PDCs and inhibits their oligomerization and enzymatic activity.Collectively,our work reveals a metabolic pathway involved in plant resistance to biotic and abiotic stresses,and a bacterial virulence strategy to promote disease and the completion of the pathogenic life cycle.展开更多
基金supported by the Strategic Priority Research Program of the Chinese Academy of Sciences(grant XDB27040204)the National Natural Science Foundation of China(grant 31571973)+1 种基金the Chinese 1000 Talents Programthe Shanghai Center for Plant Stress Biology(Chinese Academy of Sciences).
文摘Bacterial wilt caused by the soil-borne plant pathogen Ralstonia solanacearum is a devastating disease worldwide.Upon plant colonization,R.solanacearum replicates massively,causing plant wilting and death;collapsed infected tissues then serve as a source of inoculum.In this work,we show that the plant metabolic pathway mediated by pyruvate decarboxylases(PDCs)contributes to plant tolerance to bacterial wilt disease.Arabidopsis and tomato plants resp ond to R.solanacearum infection by in creasing PDC activity,and plants with deficient PDC activity are more susceptible to bacterial wilt.Treatment with either pyruvic acid or acetic acid(substrate and product of the PDC pathway,respectively)enhances plant tolerance to bacterial wilt disease.An effector protein secreted by R.solanacearum,RipAK,interacts with PDCs and inhibits their oligomerization and enzymatic activity.Collectively,our work reveals a metabolic pathway involved in plant resistance to biotic and abiotic stresses,and a bacterial virulence strategy to promote disease and the completion of the pathogenic life cycle.
