A multiphase microscopic interference system is designed to measure the height of cell which is important to the research of collective cell migration in physiology and medicine. This sys- tem can quantitatively measu...A multiphase microscopic interference system is designed to measure the height of cell which is important to the research of collective cell migration in physiology and medicine. This sys- tem can quantitatively measure cell height across a living monolayer without knowing the refractive index of cells. For the interference pattern, because the phases are all wrapped between - π to π, it is necessary to get the real phase through phase unwrapping,a method to restore the wrapped phase data of the object by using numerical calculations. Three representative algorithms are selected to unwrap the interference pattern of ceils： branch-cut method, quality-guided method and network method. Although each of them can restore the phase, their performances are obviously different. We compare these methods and find that branch-cut method needs the smallest execution time and can obtain good unwrapped patterns when noises are not serious.展开更多
In phase unwrapping, how to control the residues and cut lines is critical to unwrap ill-state wrapped phase map successfully. Some new concepts in phase unwrapping field are proposed. Firstly, the residues in three t...In phase unwrapping, how to control the residues and cut lines is critical to unwrap ill-state wrapped phase map successfully. Some new concepts in phase unwrapping field are proposed. Firstly, the residues in three types, i.e., singular residues, normal residues and virtual residues, are classified, which leads to some rational unwrapping paths. Secondly, some differences between cut-line and curved line are analyzed. And finally, experiment is carried out to compare our new enhanced Goldstein algorithm with the original Goldstein algorithm.展开更多
基金Supported by the International Technology Cooperation Projects of BIT(GZ 20110451)
文摘A multiphase microscopic interference system is designed to measure the height of cell which is important to the research of collective cell migration in physiology and medicine. This sys- tem can quantitatively measure cell height across a living monolayer without knowing the refractive index of cells. For the interference pattern, because the phases are all wrapped between - π to π, it is necessary to get the real phase through phase unwrapping,a method to restore the wrapped phase data of the object by using numerical calculations. Three representative algorithms are selected to unwrap the interference pattern of ceils： branch-cut method, quality-guided method and network method. Although each of them can restore the phase, their performances are obviously different. We compare these methods and find that branch-cut method needs the smallest execution time and can obtain good unwrapped patterns when noises are not serious.
文摘In phase unwrapping, how to control the residues and cut lines is critical to unwrap ill-state wrapped phase map successfully. Some new concepts in phase unwrapping field are proposed. Firstly, the residues in three types, i.e., singular residues, normal residues and virtual residues, are classified, which leads to some rational unwrapping paths. Secondly, some differences between cut-line and curved line are analyzed. And finally, experiment is carried out to compare our new enhanced Goldstein algorithm with the original Goldstein algorithm.
