为屠宰环节PCV3分子流行病学调查诊断提供参考,根据猪圆环病毒3型(Porcine circovirus type 3,PCV3)特异性序列设计引物,通过敏感性试验、特异性试验、重复性试验及测序验证,建立的PCR方法能扩增出649 bp的目标片段,其具有较好的敏感性...为屠宰环节PCV3分子流行病学调查诊断提供参考,根据猪圆环病毒3型(Porcine circovirus type 3,PCV3)特异性序列设计引物,通过敏感性试验、特异性试验、重复性试验及测序验证,建立的PCR方法能扩增出649 bp的目标片段,其具有较好的敏感性,可扩增的最低模板浓度为0.143 ng/μL;并用建立的PCR方法对屠宰场送检的376份组织样品进行检测结果表明,PCV3平均阳性率为12.9%。展开更多
Instead of the usual Hirota ansatz,i.e.,the functions in bilinear equations being chosen as exponentialtypes,a generalized Hirota ansatz is proposed for a (3+1)-dimensional nonlinear evolution equation.Based on theres...Instead of the usual Hirota ansatz,i.e.,the functions in bilinear equations being chosen as exponentialtypes,a generalized Hirota ansatz is proposed for a (3+1)-dimensional nonlinear evolution equation.Based on theresulting generalized Hirota ansatz,a family of new explicit solutions for the equation are derived.展开更多
文摘Instead of the usual Hirota ansatz,i.e.,the functions in bilinear equations being chosen as exponentialtypes,a generalized Hirota ansatz is proposed for a (3+1)-dimensional nonlinear evolution equation.Based on theresulting generalized Hirota ansatz,a family of new explicit solutions for the equation are derived.