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Deep learning assisted variational Hilbert quantitative phase imaging 被引量:5
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作者 Zhuoshi Li Jiasong Sun +7 位作者 Yao Fan Yanbo Jin Qian Shen Maciej Trusiak Maria Cywińska Peng Gao Qian Chen Chao Zuo 《Opto-Electronic Science》 2023年第4期1-11,共11页
We propose a high-accuracy artifacts-free single-frame digital holographic phase demodulation scheme for relatively lowcarrier frequency holograms-deep learning assisted variational Hilbert quantitative phase imaging(... We propose a high-accuracy artifacts-free single-frame digital holographic phase demodulation scheme for relatively lowcarrier frequency holograms-deep learning assisted variational Hilbert quantitative phase imaging(DL-VHQPI).The method,incorporating a conventional deep neural network into a complete physical model utilizing the idea of residual compensation,reliably and robustly recovers the quantitative phase information of the test objects.It can significantly alleviate spectrum-overlapping-caused phase artifacts under the slightly off-axis digital holographic system.Compared to the conventional end-to-end networks(without a physical model),the proposed method can reduce the dataset size dramatically while maintaining the imaging quality and model generalization.The DL-VHQPI is quantitatively studied by numerical simulation.The live-cell experiment is designed to demonstrate the method's practicality in biological research.The proposed idea of the deep learning-assisted physical model might be extended to diverse computational imaging techniques. 展开更多
关键词 quantitative phase imaging digital holography deep learning high-throughput imaging
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Quantitative phase imaging(QPI)through random diffusers using a diffractive optical network 被引量:9
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作者 Yuhang Li Yi Luo +2 位作者 Deniz Mengu Bijie Bai Aydogan Ozcan 《Light(Advanced Manufacturing)》 2023年第3期206-221,共16页
Quantitative phase imaging(QPI)is a label-free computational imaging technique used in various fields,including biology and medical research.Modern QPI systems typically rely on digital processing using iterative algo... Quantitative phase imaging(QPI)is a label-free computational imaging technique used in various fields,including biology and medical research.Modern QPI systems typically rely on digital processing using iterative algorithms for phase retrieval and image reconstruction.Here,we report a diffractive optical network trained to convert the phase information of input objects positioned behind random diffusers into intensity variations at the output plane,all-optically performing phase recovery and quantitative imaging of phase objects completely hidden by unknown,random phase diffusers.This QPI diffractive network is composed of successive diffractive layers,axially spanning in total~70λ,where is the illumination wavelength;unlike existing digital image reconstruction and phase retrieval methods,it forms an all-optical processor that does not require external power beyond the illumination beam to complete its QPI reconstruction at the speed of light propagation.This all-optical diffractive processor can provide a low-power,high frame rate and compact alternative for quantitative imaging of phase objects through random,unknown diffusers and can operate at different parts of the electromagnetic spectrum for various applications in biomedical imaging and sensing.The presented QPI diffractive designs can be integrated onto the active area of standard CCD/CMOS-based image sensors to convert an existing optical microscope into a diffractive QPI microscope,performing phase recovery and image reconstruction on a chip through light diffraction within passive structured layers. 展开更多
关键词 quantitative phase imaging Optical neural network Diffractive deep neural network Diffusive media All-optical computing
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Iterative projection meets sparsity regularization:towards practical single-shot quantitative phase imaging with in-line holography 被引量:6
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作者 Yunhui Gao Liangcai Cao 《Light(Advanced Manufacturing)》 2023年第1期35-51,共17页
Holography provides access to the optical phase.The emerging compressive phase retrieval approach can achieve in-line holographic imaging beyond the information-theoretic limit or even from a single shot by exploring ... Holography provides access to the optical phase.The emerging compressive phase retrieval approach can achieve in-line holographic imaging beyond the information-theoretic limit or even from a single shot by exploring the signal priors.However,iterative projection methods based on physical knowledge of the wavefield suffer from poor imaging quality,whereas the regularization techniques sacrifice robustness for fidelity.In this work,we present a unified compressive phase retrieval framework for in-line holography that encapsulates the unique advantages of both physical constraints and sparsity priors.In particular,a constrained complex total variation(CCTV)regularizer is introduced that explores the well-known absorption and support constraints together with sparsity in the gradient domain,enabling practical high-quality in-line holographic imaging from a single intensity image.We developed efficient solvers based on the proximal gradient method for the non-smooth regularized inverse problem and the corresponding denoising subproblem.Theoretical analyses further guarantee the convergence of the algorithms with prespecified parameters,obviating the need for manual parameter tuning.As both simulated and optical experiments demonstrate,the proposed CCTV model can characterize complex natural scenes while utilizing physically tractable constraints for quality enhancement.This new compressive phase retrieval approach can be extended,with minor adjustments,to various imaging configurations,sparsifying operators,and physical knowledge.It may cast new light on both theoretical and empirical studies. 展开更多
关键词 phase retrieval quantitative phase imaging Compressive sensing Digital holography
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Multi-prior physics-enhanced neural network enables pixel super-resolution and twin-imagefree phase retrieval from single-shot hologram
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作者 Xuan Tian Runze Li +5 位作者 Tong Peng Yuge Xue Junwei Min Xing Li Chen Bai Baoli Yao 《Opto-Electronic Advances》 SCIE EI CAS CSCD 2024年第9期22-38,共17页
Digital in-line holographic microscopy(DIHM)is a widely used interference technique for real-time reconstruction of living cells’morphological information with large space-bandwidth product and compact setup.However,... Digital in-line holographic microscopy(DIHM)is a widely used interference technique for real-time reconstruction of living cells’morphological information with large space-bandwidth product and compact setup.However,the need for a larger pixel size of detector to improve imaging photosensitivity,field-of-view,and signal-to-noise ratio often leads to the loss of sub-pixel information and limited pixel resolution.Additionally,the twin-image appearing in the reconstruction severely degrades the quality of the reconstructed image.The deep learning(DL)approach has emerged as a powerful tool for phase retrieval in DIHM,effectively addressing these challenges.However,most DL-based strategies are datadriven or end-to-end net approaches,suffering from excessive data dependency and limited generalization ability.Herein,a novel multi-prior physics-enhanced neural network with pixel super-resolution(MPPN-PSR)for phase retrieval of DIHM is proposed.It encapsulates the physical model prior,sparsity prior and deep image prior in an untrained deep neural network.The effectiveness and feasibility of MPPN-PSR are demonstrated by comparing it with other traditional and learning-based phase retrieval methods.With the capabilities of pixel super-resolution,twin-image elimination and high-throughput jointly from a single-shot intensity measurement,the proposed DIHM approach is expected to be widely adopted in biomedical workflow and industrial measurement. 展开更多
关键词 optical microscopy quantitative phase imaging digital holographic microscopy deep learning SUPER-RESOLUTION
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Deep-learning-based prediction of living cells mitosis via quantitative phase microscopy 被引量:4
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作者 Ying Li Jianglei Di +1 位作者 Li Ren Jianlin Zhao 《Chinese Optics Letters》 SCIE EI CAS CSCD 2021年第5期54-59,共6页
We present a deep learning approach for living cells mitosis classification based on label-free quantitative phase imaging with transport of intensity equation methods.In the approach,we applied a pretrained deep conv... We present a deep learning approach for living cells mitosis classification based on label-free quantitative phase imaging with transport of intensity equation methods.In the approach,we applied a pretrained deep convolutional neural network using transfer learning for binary classification of mitosis and non-mitosis.As a validation,we demonstrated the performances of the network trained by phase images and intensity images,respectively.The convolutional neural network trained by phase images achieved an average accuracy of 98.9%on the validation data,which outperforms the average accuracy 89.6%obtained by the network trained by intensity images.We believe that the quantitative phase microscopy in combination with deep learning enables researchers to predict the mitotic status of living cells noninvasively and efficiently. 展开更多
关键词 cell classification quantitative phase imaging deep learning
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Optically monitoring and controlling nanoscale topography during semiconductor etching 被引量:7
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作者 Chris Edwards Amir Arbabi +1 位作者 Gabriel Popescu Lynford L Goddard 《Light(Science & Applications)》 SCIE EI CAS 2012年第1期41-46,共6页
We present epi-diffraction phase microscopy(epi-DPM)as a non-destructive optical method for monitoring semiconductor fabrication processes in real time and with nanometer level sensitivity.The method uses a compact M... We present epi-diffraction phase microscopy(epi-DPM)as a non-destructive optical method for monitoring semiconductor fabrication processes in real time and with nanometer level sensitivity.The method uses a compact Mach–Zehnder interferometer to recover quantitative amplitude and phase maps of the field reflected by the sample.The low temporal noise of 0.6 nm per pixel at 8.93 frames per second enabled us to collect a three-dimensional movie showing the dynamics of wet etching and thereby accurately quantify non-uniformities in the etch rate both across the sample and over time.By displaying a gray-scale digital image on the sample with a computer projector,we performed photochemical etching to define arrays of microlenses while simultaneously monitoring their etch profiles with epi-DPM. 展开更多
关键词 MICROSCOPY nanoscale metrology photochemical etching quantitative phase imaging semiconductor process monitoring
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Integrated self-referencing single shot digital holographic microscope and optical tweezer 被引量:2
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作者 Subhash Utadiya Sachin Joshi +6 位作者 Nimit Patel Chetna Patel Mugdha Joglekar Vani Cahhniwal Timothy O’Connor Bahram Javidi Arun Anand 《Light(Advanced Manufacturing)》 2022年第3期205-218,共14页
Digital holographic microscopy is a single-shot technique for quantitative phase imaging of samples,yielding thickness profiles of phase objects.It provides sample features based on their morphology,leading to their c... Digital holographic microscopy is a single-shot technique for quantitative phase imaging of samples,yielding thickness profiles of phase objects.It provides sample features based on their morphology,leading to their classification and identification.However,observing samples,especially cells,in fluids using holographic microscopes is difficult without immobilizing the object.Optical tweezers can be used for sample immobilization in fluids.The present manuscript provides an overview of our ongoing work on the development of a compact,low-cost microscopy system for digital holographic imaging of optically trapped samples.Integration of digital holographic microscopy system with tweezers is realized by using the optical pickup unit extracted from DVD burners to trap microsamples,which are then holographically imaged using a highly compact self-referencing interferometer along with a low-cost,in-house developed quadrant photodiode,providing morphological and spectral information of trapped particles.The developed integrated module was tested using polystyrene microspheres as well as human erythrocytes.The investigated system offers a multitude of sample features,including physical and mechanical parameters and corner frequency information of the sample.These features were used for sample classification.The proposed technique has vast potential in opening up new avenues for low-cost,digital holographic imaging and analysis of immobilized samples in fluids and their classification. 展开更多
关键词 Digital holographic microscopy Optical tweezers Red blood cells quantitative phase imaging
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Tumor heterogeneity and therapy resistance-implications for future treatments of prostate cancer
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作者 Fiona M.Frame Amanda R.Noble +6 位作者 Sandra Klein Hannah F.Walker Rakesh Suman Richard Kasprowicz Vin M.Mann Matt S.Simms Norman J.Maitland 《Journal of Cancer Metastasis and Treatment》 CAS 2017年第1期302-314,共13页
Aim:To develop new therapies for prostate cancer,disease heterogeneity must be addressed.This includes patient variation,multi-focal disease,cellular heterogeneity,genomic changes and epigenetic modification.This requ... Aim:To develop new therapies for prostate cancer,disease heterogeneity must be addressed.This includes patient variation,multi-focal disease,cellular heterogeneity,genomic changes and epigenetic modification.This requires more representative models to be used in more innovative ways.Methods:This study used a panel of cell lines and primary prostate epithelial cell cultures derived from patient tissue.Several assays were used;alamar blue,colony forming assays,γH2AX and Ki67 immunofluorescence and comet assays.Ptychographic quantitative phase imaging(QPI),a label-free imaging technique,combined with Cell Analysis Toolbox software,was implemented to carry out real-time analysis of cells and to retrieve morphological,kinetic and population data.Results:A combination of radiation and Vorinostat may be more effective than radiation alone.Primary prostate cancer stem-like cells are more resistant to etoposide than more differentiated cells.Analysis of QPI images showed that cell lines and primary cells differ in their size,motility and proliferation rate.A QPI signature was developed in order to identify two subpopulations of cells within a heterogeneous primary culture.Conclusion:Use of primary prostate epithelial cultures allows assessment of therapies whilst taking into account cellular heterogeneity.Analysis of rare cell populations and embracing novel techniques may ultimately lead to identifying and overcoming treatment resistance. 展开更多
关键词 PROSTATE PTYCHOGRAPHY live-cell imaging primary cells quantitative phase imaging
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