QuEChERS-超高效液相色谱法同时测定硝基酚类物质方法的研究

建立了超高效液相色谱法同时测定三硝基苯酚（苦味酸PA）、三硝基间苯二酚（斯蒂酚酸TNR）和三硝基均苯三酚（TNPG）等多硝基酚类含能材料在生产环境中土壤、地面沉积物中残留的14种硝基酚类物质的检测方法。样品经改进的QuE ChERS（快速、简单、价廉、高效、耐用、安全）前处理方法一步完成提取,经C18和石墨化炭黑（GCB）净化,采用Accucore PFP色谱柱（2.1 mm×150 mm i.d.,2.6μm）分离,用梯度洗脱,流动相为乙腈＋0.02 mol·L-1乙酸铵盐缓冲溶液＋0.1%甲酸,流速0.3 mL·min-1,注射量5μL。在波长220 nm处检测14种硝基酚类物质。结果表明,14种硝基酚类物质在0.2～50 mg·L-1浓度范围内呈良好线性关系,相关系数为0.9985～0.9995,相对标准偏差为3.6%～5.9%,平均回收率为81.3%～105.7%,检出限（LOD）为0.04～0.06 mg·kg-1。14种硝基酚类物质在22 min内完成分离。所建方法有良好的重复性和精度,可用于快速测定土壤中的酚类物质。

QuEChERS-GC/ECD法分析土壤和沉积物中残留有机氯农药和多氯联苯

建立了用快速、简单、廉价、有效、可靠及安全（QuEChERS）的方法净化、GC／ECD法测定沉积物中有机氯农药和多氯联苯的分析方法。目标化合物质量浓度在1．0～100μg/L范围内线性良好，相关系数均〉0．999，检出限为0．08～0．17μg/L。以沉积物标准样品SRM1944为研究对象，用QuEChERS方法和传统方法进行提取比较，并用沉积物样品进行验证，回收率为87．8％～100．3％。方法快速、简单、可靠、安全。

Validation of the General <i>Health Questionnaire</i>(GHQ-12) adapted to a work-related context

Introduction: The aim of this study is to test the factorial structure and the internal consistency of the 12-items General Health Questionnaire adapted to work-related psychological distress (GHQW). Methods: A validated French version of the GHQ-12 was used and transformed to remind of the occupational context. A sample of 1014 workers completed the GHQW. Internal consis- tency was assessed by Cronbach’s α coefficient. The factorial structure was extracted with an exploratory factorial analysis (EFA). Results: The EFA run on the data yield to a one- factor structure explaining 60.5% of the total variance of the scale. The Cronbach’s alpha showed a very good internal consistency of the scale (α = 0.94). Conclusion: The findings support that the GHQW is a reliable and valid instrument for measuring work-related psychological distress in workers. This work-related version could find some applications in epide-miological research at work, in the study of psychosocial risk factors and in the occupational health physi- cian’s daily activity.

基于QUE$TOR软件的地面工程投资估算

石油工程投资估算软件QUE$TOR具有工程方案描述准确、计算精度高、投资数据持续更新等特点。总结了QUE$TOR软件特点和操作流程,包括项目定义、油气田特征描述、确定开发方案、刻画工程方案、计算CAPEX和OPEX、完成投资规划等。通过与实际投资进行对比,验证了软件的计算精度。QUE$TOR软件适用于项目概念设计、可行性研究以及资料较少的海外项目的投资估算。

XI AWARDS CAMBODIAN QUEEN MOTHER FRIENDSHIP MEDAL

Chinese President Xi Jinping awarded Cambodian Queen Mother,Norodom Monineath Sihanouk,the Friendship Medal of the People’s Republic of China at a ceremony at the Great Hall of the People in Beijing on November 6.Queen Mother Monineath is an important witness and promoter of China-Cambodia friendship,who has forged a tight bond with China since she accompanied Norodom Sihanouk,then Cambodian prince,to China in 1958 for the first time,Xi said when delivering a speech.

作为关系代词及连词的法语QUE与英语THAT的比较——基于认知语法的角度

从认知语法的角度对法语que与英语that的共同点、差异性与惯用法进行全面系统地比较:在句法功能上两者都可以作连词与关系代词;作连词时,需要注意que的一些特殊用法以及两者后面所跟从句语式的差别;作代词时,在关注两者共同点的同时要注意that的代替性与可省略性。法语学习者可利用法语和英语两种语言在句法上的象似性提高学习效率。

Empirical studies about quercetin increasing chemosensitivity on human lung adenocarcinoma cell line A549

Objective： The present study was designed to investigate whether quercetin exerts increasing chemosensitivity on human lung adenocarcinoma cells when quercetin combined with cisplatin （DDP） and vincristine （VCR） in vitro respectively and its possible antitumor mechanism. To provide experimental proof for clinical combination application. Methods： Using intermittent administration of high dose VCR, human lung adenocarcinoma sensitive cell line （A549/S） was induced to VCR- resistant human lung adenocarcinoma cell line （A549NCR）. MTT assay was adapted for examing the 50% inhibition （IC50） value of DDP and VCR on A549/S and A549/VCR when quercetin combined with DDP and VCR respectively. Results： IC50 of DDP on A549/S and A549/VCR was 10.18 and 12.35 mg/L, and the IC50 of VCR on the two cell lines was 1.21 and 12.77 rag/L, respectively. The resistance fold of A549/VCR on VCR and DDP was 10.55 and 121, respectively. When quercetin at concentration of 50, 100 and 200 pmol/L in combination with DDP and VCR respectively, the IC50 of DDP and VCR on A549/S and A549/VCR were obvious decreased （P 〈 0.05 - P 〈 0.01）. Conclusion： The experiment results suggested that quercetin could increase the chemosensitivity and partly revise the resistance of A549NCR.

EFFECTS OF QUERCETIN ON MEMBRANE FLUIDITY OF INJURED VASCULAR ENDOTHELIAL CELLS WITH HYPOXIA AND THE LACK OF GLUCOSE

Objective To study the effects of different concentrations of Quercetin on nitric oxide (NO) production and membrane fluidity of the injured human umbilical vein vascular endothelial cell line(ECV 304) with hypoxia and the lack of glucose. Methods The experiments were performed in the culture of ECV 304 injured with hypoxia and the lack of glucose in vitro. The releases of intracellular lactate dehydrogenase(LDH) of ECV 304 was measured with automatic biochemistry analysis. NO level of ECV 304 was monitored with colorimetry. The membrane fluidity of ECV 304 was measured with the fluorescence polarization method. Results After ECV 304 was cultured in hypoxia and the the lack of glucose for 24 hours, the release of LDH and the membrane fluidity were increased significantly; NO level was decreased. Preincubation of ECV 304 with 20, 80,160 μ mol·L -1 of Quercetin for 24 hours reduced LDH activity, membrane fluidity and increased the level of NO in hypoxia and the lack of glucose induced ECV 304. Conclusion These results demonstrate that Quercetin can produce the protective effect on hypoxia and the lack of glucose induced injury of ECV 304 by increasing release of NO and changing membrane fluidity.

应用Omni pa Que脊髓造影的体会与评价

一、造影方法1.下行性造影患者俯卧位,双手握紧检查床的固定把手、下肢伸直、固定双侧踝关节、头低5°,前屈,电视监视下定位,缓慢的将造影剂注入椎管内。下行性造影检查,常用于颈椎病,颈椎外伤,上胸椎血管畸形、肿瘤、外伤等疾病。摄颈椎正位。

新发现的月球陨石QUE93069

新发现的月球陨石ＱＵＥ９３０６９为了考察和研究太阳系形成演化及人类对宇宙空间的开发利用，美国和前苏联等先进国家不惜花费巨资实施Ａｐｏｌｌｏ登月和飞船探测计划，从月球取回昂贵的月岩标本。然而，截止１９９１年，从美国、日本和欧洲等南极科学考察队在南极地区...

ERATO,REINE D’ARMÉNIE,ÉTUDE HISTORIQUE ET NUMISMATIQUE

Introduction Les découvertes numismatiques récentes justifient un réexamen de la période mal connue des dernières années de la dynastie artaxiade en Grande-Arménie(fondée vers 189 av.J.-C.par Artaxias Ier),et plus particulièrement la période concernant le ou les règnes d’Erato,reine d’Arménie.

CLINICAL OBSERVATION ON THE TREATMENT OF KNEE OSTEOARTHRITIS BY ACUPUNCTURE IN BA-HE TECHNIQUE

Objective: To observe the clinical therapeutic effect of Ba-He techniqu e of acupuncture in the treatment of knee osteoarthritis. Methods: A total of 90 cases of knee osteoarthritis were evenly randomized into Ba-He technique group (observation group) and common technique group (control group), with 45 cases in each group. Zusanli (足三里 ST 36), Heding (鹤顶 EX-LE 2) and Dubi (犊鼻 ST 35 ) were punctured respectively in Ba-He technique and common technique in the two gr oups. The therapeutic effects of the two groups were compared after one course o f treatment (10 sessions) in accordance with Japanese assessment criteria for kn ee-joint functions. Results: After treatment, among the 74 and 7 1 affected knees in the observation and control groups, the therapeutic effect was excellent in 42 (56.8%) and 26 (36.6%) knees, fine in 14 (18.9%) and 20 (28.2%), OK in 10 (13 .5%) and 11 (15.5%), and poor in 8 (10.8%) and 14 (19.7%) respectively. The ther apeutic effect of the observation group was significantly better than that of co ntrol group (P<0.01). Conclusion: The Ba- He technique of acupuncture applied to the above-mentioned three acupoints exerted remarkable therapeutic effect for k n ee osteoarthritis, which is obviously better than that of the common needling te chnique.

浅谈QUE$TOR软件的应用

随着我国海外油气业务的不断壮大和发展,工程投资估算对于新项目未来的盈利能力起着至关重要的影响。对于一些前端工程设计的项目评价工作,由于已知参数和条件比较少,如何客观准确的对项目进行评价,是项目评价人员应该深刻思考的问题。美国IHS Markit公司开发的QUE$TOR软件,因其良好的精确度和可靠性得到了很多国际石油公司的认可。QUE$TOR在各个产油国和地区拥有自己的研发团队,其后台成本数据库每年更新两次,这种强大的专业信息搜集和整理的团队是很多国家不具备的,因此该软件将会在各国际石油公司中变得越来越重要。QUE$TOR软件适用于项目概念设计、可行性研究及资料较少的海外项目。本文目的是通过对石油工程投资估算软件QUE$TOR的流程研究,从中找出国际化石油公司对于项目投资和费用估算的关键节点,有助于我国石油公司与其竞争时了解竞争对手的最低报价,及调整投标策略。

Information in the Structure of Reality:the Scientific Worldview and the Fundamental Question of Philosophy

We offer a new understanding of the scientific world and the contents of the basic question of philosophy,which is based on an analysis of the phenomenon of the specific information in the various components of the structure of reality.It is shown that,in addition to material objects,processes,and events,in the real world are also intangible objects,processes and conditions that are in its information content.So the basic question of philosophy is proposed to treat the relationship as a tangible and intangible component of reality.More succinctly it can be formulated as the ratio of matter and information.This allows you to point to some new challenges studying the phenomenon of information in various components of the structure of reality.

Single-cell profiling reveals a potent role of quercetin in promoting hair regeneration

Hair loss affects millions of people at some time in their life,and safe and efficient treatments for hair loss are a significant unmet medical need.We report that topical delivery of quercetin(Que)stimulates resting hair follicles to grow with rapid follicular keratinocyte proliferation and replenishes perifollicular microvasculature in mice.We construct dynamic single-cell transcriptome landscape over the course of hair regrowth and find that Que treatment stimulates the differentiation trajectory in the hair follicles and induces an angiogenic signature in dermal endothelial cells by activating HIF-1αin endothelial cells.Skin administration of a HIF-1αagonist partially recapitulates the pro-angiogenesis and hair-growing effects of Que.Together,these findings provide a molecular understanding for the efficacy of Que in hair regrowth,which underscores the translational potential of targeting the hair follicle niche as a strategy for regenerative medicine,and suggest a route of pharmacological intervention that may promote hair regrowth.

槲皮素对Erastin诱导的软骨细胞铁死亡的影响

目的探究槲皮素(quercetin,Que)对Erastin诱导的软骨细胞铁死亡模型中铁死亡的影响及可能的机制。方法使用Erastin诱导C28/I2软骨细胞铁死亡模型。采用不同浓度的Que处理C28/I2软骨细胞,分别用MTT法检测细胞活性,LDH法检测细胞毒性。Western blot检测软骨细胞Prdx6和铁死亡相关蛋白ACSL4、GPX4的表达水平。流式细胞术检测软骨细胞脂质ROS的产生情况。RH123染色法检测软骨细胞线粒体膜电位的变化情况。RT-qPCR检测软骨细胞Prdx6 mRNA表达水平。免疫荧光染色法观察铁死亡相关蛋白ACSL4、GPX4的表达变化情况。结果与Erastin诱导的铁死亡模型组相比,Que可以明显提高C28/I2软骨细胞活性,降低细胞毒性。降低铁死亡相关蛋白ACSL4的表达,升高GPX4的表达。同时抑制软骨细胞脂质ROS的产生,并增强其线粒体膜电位。此外,与Control组相比,Erastin组Prdx6的表达明显降低,而使用Que可以上调Prdx6的表达。同时,在使用Prdx6的抑制剂MJ33后,Que对软骨细胞铁死亡的抑制作用下降。结论Que可以抑制Erastin诱导的C28/I2软骨细胞铁死亡,并可能通过上调Prdx6抑制软骨细胞铁死亡,从而发挥保护软骨细胞的作用。

舞剧《铜雀伎》叙事研究

舞剧《铜雀伎》作为中国当代舞蹈发展史中最具代表性的剧作之一,一方面体现了20世纪80年代以来中国舞剧向内求索的民族艺术精神;另一方面为中国舞剧的戏剧建构与叙事实践做出了积极的贡献。文章运用叙事理论挖掘舞蹈艺术中的戏剧叙事形式,从文本叙事、叙事组织及人物叙事三个方面,对舞台表现的戏剧性要素与结构性要素进行分析,理清“戏”之于“舞”的前置性规约与重要作用。同时,在《铜雀伎》中探知历史文化中的传统戏剧美学基因,以期探索民族舞剧的叙事表意机制的构建,让舞剧艺术更好地讲述中国故事、传承民族文化精神。

基于Super-SILAC定量技术系统解析槲皮素调控巨噬细胞的组蛋白翻译后修饰图谱

槲皮素(quercetin,Que)是一种具有多种生物活性的黄酮类化合物,在自然界中分布广泛,具有抗炎和抗肿瘤等生物活性。研究发现,槲皮素的抗炎活性与其对组蛋白翻译后修饰的调控密切相关。然而,槲皮素调控组蛋白翻译后修饰发挥抗炎作用的精细机制尚未有详细报道。为了探讨槲皮素调控组蛋白翻译后修饰发挥抗炎活性的作用机制,我们首先评估了槲皮素对M1型巨噬细胞极化的影响,结果发现,槲皮素可以明显下调M1型巨噬细胞的白细胞介素-1β(interleukin-1β,IL-1β)和白细胞介素-6(interleukin-6,IL-6)水平;其次,利用生物质谱的细胞稳定同位素标记(stable isotope labeling by amino acids in cell culture,SILAC)技术衍生的super-SILAC方法,进行了组蛋白翻译后修饰的解析,系统定量分析了槲皮素处理后的巨噬细胞中组蛋白修饰水平的全局性变化;最终,共定量到30个组蛋白修饰位点,其中包括12个发生下调的组蛋白乙酰化修饰位点和4个发生上调的甲基化修饰位点(fold change>1.2,P<0.05);进一步对部分差异变化位点进行了蛋白质免疫印迹(western blot,WB)验证,其差异变化与质谱结果一致性良好。综上,本研究系统解析了槲皮素调控巨噬细胞的组蛋白翻译后修饰图谱,为槲皮素抗炎作用机制的研究提供了可靠的数据参考和新的线索。

阳雀花纹样的艺术特征及在服饰中的设计应用

阳雀花纹样是中国土家族典型的纹样图案。土家族独特的民俗风情为阳雀花纹样增添了热情活力,而新时代需要在产品中融入新的设计元素以适应现代化的市场需求。采用文献研究法着重探析了阳雀花纹样的文化内涵、创新应用和传承意义,以发扬这一传统纹样。对传统纹样中的阳雀花纹样进行深入研究,并对阳雀花的元素进行提取和创新性设计运用。一方面深入挖掘阳雀花纹的文化价值,使古老文明重新焕发光彩,得到继承和发展;另一方面,重新提取阳雀花纹的元素进行产品设计,为产品设计提供新的灵感,为文化产品赋能以及增加其附加价值,使古老的传统艺术在新时期焕发出新的美学价值。