Quantitative trait loci identification reveals zinc finger protein CONSTANS-LIKE 4 as the key candidate gene of stigma color in watermelon(Citrullus lanatus)

Stigma color is a critical agronomic trait in watermelon that plays an important role in pollination.However,there are few reports on the regulation of stigma color in watermelon.In this study,a genetic analysis of the F2 population derived from ZXG1553(P1,with orange stigma)and W1-17(P2,with yellow stigma)indicated that stigma color is a quantitative trait and the orange stigma is recessive compared with the yellow stigma.Bulk segregant analysis sequencing(BSA-seq)revealed a 3.75 Mb segment on chromosome 6 that is related to stigma color.Also,a major stable effective QTL Clqsc6.1(QTL stigma color)was detected in two years between cleaved amplified polymorphic sequencing(CAPS)markers Chr06_8338913 and Chr06_9344593 spanning a~1.01 Mb interval that harbors 51 annotated genes.Cla97C06G117020(annotated as zinc finger protein CONSTANS-LIKE 4)was identified as the best candidate gene for the stigma color trait through RNA-seq,quantitative real-time PCR(qRT-PCR),and gene structure alignment analysis among the natural watermelon panel.The expression level of Cla97C06G117020 in the orange stigma accession was lower than in the yellow stigma accessions with a significant difference.A nonsynonymous SNP site of the Cla97C06G117020 coding region that causes amino acid variation was related to the stigma color variation among nine watermelon accessions according to their re-sequencing data.Stigma color formation is often related to carotenoids,and we also found that the expression trend of ClCHYB(annotated asβ-carotene hydroxylase)in the carotenoid metabolic pathway was consistent with Cla97C06G117020,and it was expressed in low amounts in the orange stigma accession.These data indicated that Cla97C06G117020 and ClCHYB may interact to form the stigma color.This study provides a theoretical basis for gene fine mapping and mechanisms for the regulation of stigma color in watermelon.

Zinc finger protein 296 promotes hepatocellular carcinoma progression via intervening interaction between macrophages and B cells

Objective:Hepatocellular carcinoma(HCC)is a prevalent malignancy with poor survival.Different cell types in the tumor microenvironment participate in the tumorigenesis and progression of HCC.This study aimed to analyze the immune microenvironment of HCC and its relationship with clinical outcomes.Methods:We analyzed HCC RNA-seq for cell type identification and prognosis by estimating relative subsets of RNA transcripts using CIBERSORTx.The interaction between B cells and macrophages in HCC was analyzed using a Hepa1-6 orthotopic transplantation mouse model and flow cytometry.The effect of Zinc finger protein 296(ZNF296)on the interaction of B cells and macrophages was verified using human HCC tissues analyzed through western blot,quantitative real-time polymerase chain reaction(qPCR),and multiplex immunofluorescence.A comparative analysis of immune cells associated with HCC prognosis was performed using RNA-seq data from The Cancer Genome Atlas(TCGA),bulk multimodal data,and single-cell transcriptomic data from existing HCC single-cell transcriptomic data employing the Single Cell Inferred Site Specific Omics Resource for Tumor Microenvironments(SCISSOR).Results:Liver hepatocellular carcinoma(LIHC)RNA-seq analysis of TCGA showed that high eosinophil infiltration promoted HCC progression.The proportion of B cells correlated with that of macrophages(r=−0.24)and affected the infiltration and programmed death ligand 1(PD-L1)expression of macrophages in HCC.ZNF296 may participate in the interaction between B cells and macrophages to accelerate the HCC progression by regulating PAFAH1B3 and H2AFX.Moreover,ZNF296 expression positively correlated with LAG3(r=0.27)and CTLA4(r=0.31)expression levels.Among the immune cell phenotypes related to survival and death identified by SCISSOR analysis,T cells correlated with an excellent prognosis of HCC.The normal function of liver and dendritic cells was also associated with a good prognosis in HCC.Conclusions:This study analyzed the interaction of the immune microenvironment with HCC prognosis,identifying ZNF296 as a promising diagnostic and therapeutic target for HCC.

Analysis of the Effect of Intensive Nursing Care on Improving Sleep Quality During the Perioperative Period of Severed Finger Reimplantation

Objective:To investigate the clinical effect of perioperative intensive nursing on patients who underwent severed finger reimplantation and its influence on sleep quality.Methods:62 patients who underwent severed finger reimplantation between January 2023 and December 2023 were included.They were divided into two groups:the observation group(n=31)and the control group(n=31).The control group received basic nursing interventions,while the observation group received intensive nursing care.The comparison parameters included visual analog pain score(VAS),incidence of vascular crises,length of hospitalization,Pittsburgh Sleep Quality Index(PSQI),Hamilton Depression Score(HAM-D),Hamilton Anxiety Score(HAM-A),and patient satisfaction.Results:Postoperative VAS score,incidence of vascular crisis,hospitalization time,PSQI score,and HAM-A and HAM-D scores of the observation group were lower than those of the control group(P<0.05).Meanwhile,the patient satisfaction of the observation group was higher than that of the control group(P<0.05).Conclusion:Perioperative intensive nursing care for patients undergoing severed finger reimplantation demonstrates significant benefits.It reduces postoperative pain,lowers the incidence of vascular crises,shortens hospitalization durations,enhances sleep quality,alleviates negative emotions,and improves nursing satisfaction.These findings underscore the importance and applicability of such care practices.

Diagnostic value of methylated branched chain amino acid transaminase 1/IKAROS family zinc finger 1 for colorectal cancer

BACKGROUND The diagnostic value of combined methylated branched chain amino acid transaminase 1(BCAT1)/IKAROS family zinc finger 1(IKZF1)in plasma for colorectal cancer(CRC)has been explored since 2015.Recently,several related studies have published their results and showed its diagnostic efficacy.AIM To analyze the diagnostic value of methylated BCAT1/IKZF1 in plasma for screening and postoperative follow-up of CRC.METHODS The candidate studies were identified by searching the PubMed,Embase,Cochrane Library,CNKI,and Wanfang databases from May 31,2003 to June 1,2023.Sensitivity,specificity,and diagnostic accuracy were calculated by merging ratios or means.RESULTS Twelve eligible studies were included in the analysis,involving 6561 participants.The sensitivity of methylated BCAT1/IKZF1 in plasma for CRC diagnosis was 60%[95%confidence interval(CI)53-67]and specificity was 92%(95%CI:90-94).The positive and negative likelihood ratios were 8.0(95%CI:5.8-11.0)and 0.43(95%CI:0.36-0.52),respectively.Diagnostic odds ratio was 19(95%CI:11-30)and area under the curve was 0.88(95%CI:0.85-0.91).The sensitivity and specificity for CRC screening were 64%(95%CI:59-69)and 92%(95%CI:91-93),respectively.The sensitivity and specificity for recurrence detection during follow-up were 54%CONCLUSION The detection of methylated BCAT1/IKZF1 in plasma,as a non-invasive detection method of circulating tumor DNA,has potential CRC diagnosis,but the clinical application prospect needs to be further explored.

介导植物干旱应答的RING finger类型泛素连接酶的研究进展

干旱是影响植物生长和农作物产量的重要环境因素。泛素连接酶E3是蛋白质泛素化修饰的关键酶之一,对于植物生长发育和干旱胁迫应答具有重要的调控作用。RING finger类型泛素连接酶是其成员最多的一种类型。本文主要概述了植物对干旱胁迫的响应机制及植物中的RING finger类型E3,并详细综述了RING finger类型E3介导植物干旱胁迫应答的研究进展,可以供大家参考。

Nutritional Composition and Anti-Nutrient Levels in Raw and Processed Varieties of Finger Millet Promoted for Nutritional Security

Finger millet (FM) is rich in nutrients such as minerals, vitamins, and amino acids. However, the levels of nutrients and their bioaccessibility depend on the variety, the levels of ant nutrients, the chemical form of nutrients, and the type of processing methods used. The study determined the levels of selected nutrients, anti-nutrients, and bioaccessibility in raw and processed varieties of finger millet being developed by the Kenya Agricultural and Livestock Research Organization (KALRO) in Kenya. Raw finger millet seeds from KALRO Centers in Kenya were processed by malting for 60 hours and roasting at 110°C for 5 minutes as the optimal conditions. Levels of minerals were determined by AAS and AES, anti-nutrients by UV-visible spectrophotometer, proteins by the Pierce kit method, and vitamins by HPLC. The IE4115 and IE3779 showed the highest levels of nutrients and lowest levels of antinutrients hence preferred for processing and bioaccessibility studies. The level (mg/100 g) of selected minerals;K, Cr3+, Mg, Ca, P, Fe, and Zn were found to be highest in the following varieties of the FM;IE3779 (688.519 ± 1.57), IE 4115 (1.29 ± 0.07), IE4115 (294.38 ± 1.93), IE3779 (466.67 ± 4.17), IE4115 (250.92 ± 0.33), KERICHO P (16.98 ± 0.05) and IE4115 (64.10 ± 2.35) respectively. For β-carotene, vitamin B, B2, B3, B6 and B9 the levels were highest in the following varieties of FM;KAKW3 (0.023 ± 0.02), IE4115 (14.85 ± 0.16), IE4115 (12.998 ± 0.04), IE4115 (5.843 ± 0.07), IE3779 (0.06 ± 0.04) and KAKW4 (9.832 ± 0.08). Phytates, tannins, phenols, and oxalates were found to be lowest in the following varieties: IE3779 (14.20 ± 2.90, IE4115 (27.83 ± 0.73), NKFM1 (9.69 ± 0.07) and IE4115 (0.25 ± 0.01). The highest bioaccessibility values reported for K, Mg, Ca, P, Cr3+, Fe, and Zn were 89.53% (malting, IE3779), 49.28% (malting, IE4115), 60.41% (Malting, IE4115), 69.40% (malting, IE4115), 12.9% (malting, IE4115), 59.84% (malting, KAKW3) and 66.89% (roasting, IE3779) respectively (Table 8). For beta carotene, vitamin B1, B2, B3, B6 and B9 the values were 73.33% (malting, p224), 78.84% (malting, IE4115), 78.34 (malting, IE3779), 97.63% (malting, IE4115), 91.64% (malting, IE4115), and 77.52% (roasting, IE4115) (table The result on levels and bioaccessibility showed that IE4115 and IE3779 varieties were more nutritious and therefore should be promoted for nutritional security.

Description of the Factors Associated with Trigger Finger in Patients Treated at Petroleos Mexicanos “Pemex”

Background: The trigger finger, also known as stenosing tenosynovitis of the flexor tendon, is a common cause of hand disability;This can result in significant limitations in certain daily living activities. It is possible to treat this condition with conservative or surgical methods. Methods: This is a retrospective, observational, cross-sectional and descriptive study. The source population consisted of all patients with a diagnosis of trigger finger who had been treated in the department of plastic and reconstructive surgery of our institution between 2020 and 2023. The primary objective of this study is to determine the prevalence and characterize the clinical and surgical characteristics of the population affected by trigger finger in our hospital. Results: The total population was 237 patients, of which 165 were women (70%) and 72 men (30%). The most affected hand was the right hand, with a total of 158 patients (67%). In the right hand, the middle finger was the most affected (45%). Of the 237 patients, 80 of them had obesity (34%), 86 had type 2 diabetes mellitus (36%), 95 had systemic arterial hypertension (40%). Open technique was performed in 190 (80%) patients, while percutaneous technique was performed in 47 (20%) of them. Conclusion: The female sex was twice as frequent, and the middle finger and thumb were the most affected. Most of the patients received surgical treatment with open approach and percutaneous approach in 20% of them.

Investigation of Experimental Devices for Finger Active and Passive Tactile Friction Analysis

Complicated tribological behavior occurs when human fingers touch and perceive the surfaces of objects.In this process,people use their exploration style with different conditions,such as contact load,sliding speed,sliding direction,and angle of orientation between fingers and object surface consciously or unconsciously.This work addressed interlaboratory experimental devices for finger active and passive tactile friction analysis,showing two types of finger movement.In active sliding experiment,the participant slid their finger freely against the object surface,requiring the subject to control the motion conditions themselves.For passive sliding experiments,these motion conditions were adjusted by the device.Several analysis parameters,such as contact force,vibration acceleration signals,vibration magnitude,and fingerprint deformation were recorded simultaneously.Noticeable friction differences were observed when comparing active sliding and passive sliding.For passive sliding,stick-slip behavior occurred when sliding in the distal direction,evidenced by observing the friction force and the related deformation of the fingerprint ridges.The employed devices showed good repeatability and high reliability,which enriched the design of the experimental platform and provided guidance to the standardization research in the field of tactile friction.

Considerations for gluten free foods-pearl and finger millet processing and market demand

The market demand for gluten free foods is increasing due to frequent incidences of celiac disease and increasing awareness on consumption of gluten free foods.Millets have become the major constituent of diet as they are gluten-free and also excellent sources of micro and macro nutrients such as vitamins,minerals,dietary fibers and phenolic compounds.Among various millets,the finger millet and the pearl millet are the two most important and common millet varieties grown extensively.Since,they are regarded as the staple foods of the poor and vulnerable populations,development of new products and improvements in their nutritional quality will aid in the general health of these population.Processing of millets and production of variable gluten-free ready-to-eat and nutritional supplements has increased their market value in the recent years.Furthermore,processing can also help in shelf-life extension of the millets with nutritional enrichment,expanding its markets to non-traditional millet consumers.In this context,the present review is aimed to focus on the current processing methods to develop products from the two millet varieties that are gluten free and outline their nutritional benefits.

Biometric Finger Vein Recognition Using Evolutionary Algorithm with Deep Learning

In recent years,the demand for biometric-based human recog-nition methods has drastically increased to meet the privacy and security requirements.Palm prints,palm veins,finger veins,fingerprints,hand veins and other anatomic and behavioral features are utilized in the development of different biometric recognition techniques.Amongst the available biometric recognition techniques,Finger Vein Recognition(FVR)is a general technique that analyzes the patterns of finger veins to authenticate the individuals.Deep Learning(DL)-based techniques have gained immense attention in the recent years,since it accomplishes excellent outcomes in various challenging domains such as computer vision,speech detection and Natural Language Processing(NLP).This technique is a natural fit to overcome the ever-increasing biomet-ric detection problems and cell phone authentication issues in airport security techniques.The current study presents an Automated Biometric Finger Vein Recognition using Evolutionary Algorithm with Deep Learning(ABFVR-EADL)model.The presented ABFVR-EADL model aims to accomplish bio-metric recognition using the patterns of the finger veins.Initially,the presented ABFVR-EADL model employs the histogram equalization technique to pre-process the input images.For feature extraction,the Salp Swarm Algorithm(SSA)with Densely-connected Networks(DenseNet-201)model is exploited,showing the proposed method’s novelty.Finally,the Deep-Stacked Denoising Autoencoder(DSAE)is utilized for biometric recognition.The proposed ABFVR-EADL method was experimentally validated using the benchmark databases,and the outcomes confirmed the productive performance of the proposed ABFVR-EADL model over other DL models.

Finger compartment syndrome due to a high-pressure washer injury:A case report

BACKGROUND Although the finger compartment syndrome is not common,it compresses the neurovascular bundles in a limited space and blocks blood flow to the fingers,causing necrosis of the fingertips.Finger fasciotomy through unilateral or bilateral midline release of the finger can achieve decompression of the finger compartment.Herein,we report a case of the compartment syndrome in a finger injury caused by a high-pressure water flow which is commonly used in car washing stations.CASE SUMMARY A 60-year-old man injured his right middle finger while using a high-pressure washer at a car washing station.The patient complained of severe pain in his middle finger and a 0.2 cm punctured open wound on the volar side of the distal phalangeal joint of the middle finger.The fingertip was pale,numb,and characterized by severe swelling and a limited range of motion.Finger radiography showed that there was no fracture in the finger.Digital decompression was performed through finger fasciotomy by bilateral midline incision.On the second day after surgery,the color of the fingertip returned to pink,swelling was resolved,and the range of motion returned to normal.The sensation of the fingertip was completely restored,and the capillary refill test and pinprick test were positive.CONCLUSION The fingertip compartment syndrome can be caused by a high-pressure water flow damage to the fingers when using high-pressure washers at a car washing station.To avoid finger necrosis,rapid diagnosis of the finger compartment syndrome and appropriate digital decompression are essential to better outcome.

Prognostic role of ring finger and WD repeat domain 3 and immune cell infiltration in hepatocellular carcinoma

We have found that the expression of ring finger and WD repeat domain 3(RFWD3)is significantly higher in unpaired and paired hepatocellular carcinoma(HCC)tissues than in normal tissues.Moreover,this expression has a significant correlation with the infiltration level of 14 immune cell types and when the detected RFWD3 expression levels were grouped as high and low,a prominent difference was revealed for overall survival,disease-specific survival,and progression-free interval.Through statistical analysis(univariate Cox),we were also able to identify RFWD3 as an independent prognostic element for HCC,with RFWD3 having an ability to accurately predict HCC prognosis(area under the curve of 0.863).Finally,we have generated prognostic nomograms for probabilities of 1-,3-and 5-year overall survival in HCC via integrating the factors of age,pathologic stage,alpha-fetoprotein level,and RFWD3 expression.

Triggering,clicking,locking and crepitus of the finger:A comprehensive overview

Triggering,locking,clicking,and crepitus of the fingers are common symptoms patients present with.Even though crepitus and triggering can occur as part of the same underlying diagnosis,it is important to differentiate between them,as they usually indicate different possible diagnoses.The differential diagnoses that should be considered include trigger finger,metacarpophalangeal joint(MCPJ)arthritis,fractures or dislocations,extensor digitorum communis subluxation or dislocation,locked MCPJ,avascular necrosis of the metacarpal head,and Dupuytren’s disease.A thorough clinical examination with appropriate special investigations can permit the clinician to make the correct diagnosis.Appropriate management of a confirmed diagnosis is successful in providing symptomatic improvement.

The Effect of Modified Postoperative Treatment on Finger Replantation Survival Rate

Objective:To explore the clinical effect of modified treatment after finger replantation and its impact on the survival rate of replantation.Methods:The research was conducted from March 2022 to March 2023.A total of 58 patients who underwent finger replantation at our hospital were selected.These patients were divided into two groups using the digital table grouping method:the research group(n=29)and the control group(n=29).Patients in the control group received standard treatment following finger replantation,while patients in the study group received modified treatment after the procedure.The incidence of vascular crisis and the survival rate of replantation were compared between the two groups.Results:The incidence of vascular crisis in the study group was lower than that in the control group(P<0.05);the replantation survival rate in the study group was higher than that in the control group(P<0.05).Conclusion:Modified treatment after replantation of severed fingers can reduce the incidence of vascular crisis replantation and improve the survival rate of replantation,so it should be popularized and applied in medical institutions.

混合阻塞变刚度软手指设计与实验

为了解决单一阻塞介质变刚度软机械手抓取复杂外形物体效果不佳的问题,受人体手指结构启发,设计了一种仿指腹结构的混合阻塞变刚度软手指。将其设计为双层结构,外层为气动驱动器,内层为基于主动层干扰与被动颗粒阻塞的混合阻塞变刚度层,使得软手指自动贴合被抓取对象,实现主动驱动的被动适应,并通过调整刚度实现可靠抓取。基于传统缝纫工艺,采用超弹性硅胶材料制造软手指。采用Euler-Bernoulli梁理论和虚功原理,建立了基于悬臂梁结构下的多材料软手指的刚度控制模型,并据此提出增大整体刚度的材料筛选方法。弯曲特性实验表明软手指的弯曲性能优异。变刚度和抓取实验结果表明,混合阻塞软手指刚度增大4.6倍,建立的刚度模型最大相对误差为3.4%;在抓取对象表面粗糙度增大的条件下,软手指拉脱力仍增大17%,达到1.7 N;相较于单阻塞介质软手指,抓取成功率和承载能力均得到显著提高。

手指鲍恩病误诊3例

报告3例手指鲍恩病。例1.患者男,80岁。右手无名指红斑、丘疹及脱屑2余年,疼痛2个月,误诊为乳房外Paget病。例2.患者男,88岁。左手中指红斑、斑块及糜烂10余年,误诊为基底细胞癌。例3.患者男,37岁。右手示指红斑、斑块、糜烂伴瘙痒2年,误诊为湿疹。3例患者皮损组织病理检查均表现为表皮角化过度伴角化不全,表皮全层细胞极性紊乱,可见核大深染的细胞,病理性核分裂象增多;真皮浅层可见不等量淋巴细胞及组织细胞浸润。3例患者均诊断为手指鲍恩病。

玉米免耕播种机振动特性及对排种器排种性能的影响

为研究玉米免耕播种机振动特性以及振动对排种器漏播指数和重播指数的影响,搭建一套由振动加速度传感器、电荷放大器、USB采集卡组成的播种作业振动测试系统,在玉米免耕播种机免耕地表作业时进行振动测试采集播种作业的振动信号。因田间工作环境复杂,使用经典滤波法中的IIR滤波器滤除所采集振动信号中其他高频干扰信号,对田间采集振动信号进行时域分析,均方值作为时域分析指标值。结果表明:振动加速度均方根随着作业速度和旋耕机转速的增加呈线性增加,旋耕机转速和作业速度是引起玉米免耕播种机振动的主要因素,影响顺序为:旋耕机转速>作业速度。使用二次积分法对田间采集信号进行分析,得出播种机作业时最大振动位移为16.004mm,对振动信号进行频域分析,使用直接法求出功率谱密度。结果表明:玉米免耕播种机振动频率主要在0~100Hz之间,频率分布与旋耕机转速影响较大。根据田间振动信号所得工作参数搭建振动试验台,以播种机振动频率、振动幅值、作业速度为试验因素,合格指数、漏播指数为评价指标进行3因素3水平响应面试验。结果表明:作业速度、振动幅值和振动频率对合格指数和漏播指数影响较为显著。各因素对合格指数影响顺序为:振动幅值、作业速度、振动频率;各因素对漏播指数影响顺序为:振动幅值、振动频率、作业速度。研究结果可为降低玉米免耕播种机振动和优化指夹式排种器提供理论参考。

改良拇指桡背侧皮神经营养血管皮瓣在拇指缺损重建中的应用

目的探讨改良拇指桡背侧皮神经营养血管皮瓣在拇指缺损重建的手术方法和临床效果。方法回顾性分析2019年4月—2022年8月中国人民解放军联勤保障部队第九八七医院烧伤整形显微外科收治拇指末节离断伤无法再植或者再植失败患者7例,男性6例,女性1例;年龄20~51岁,平均39.4岁;左手4例,右手3例;挤轧伤5例,切割伤2例;因指体毁损严重无法再植4例,再植失败后行皮瓣修复3例。剔除离断指体近端的坏死组织后保留指骨,采用、改良大面积拇指桡背侧皮神经营养血管皮瓣包裹外露指骨重建拇指末节,供区行植皮覆盖。出院后随访皮瓣外观、感觉,以及供区和手功能恢复情况。结果患者缺损面积为2.5 cm×4.5 cm~3.0 cm×6.0 cm,皮瓣切取面积为2.5 cm×5.0 cm~3.5 cm×6.5 cm。7例皮瓣全部存活,供区植皮全部成活。术后随访3~20个月,平均4.6个月。术后3个月,皮瓣外形饱满,无臃肿,质地满意,感觉初步建立。根据中华医学会手外科分会上肢部分功能评定试用标准评价手指功能:优5例,良1例,可1例,差1例,优良率85.7%。结论经改良后进一步将拇指桡背侧皮神经营养血管皮瓣切取面积扩大,包裹外露指骨重建拇指末节,皮瓣血运可靠,手术操作简便、成功率高,供区损伤小,临床效果满意,值得推广。

斑点叉尾鮰ZBTB38的原核表达、多克隆抗体制备及应用

为获得斑点叉尾鮰(Ictalurus punctatus)ZBTB38的多克隆抗体,并研究其在性腺中的表达情况,将斑点叉尾鮰zbtb38基因部分序列经密码子优化后连接至pET32a(+)载体,构建重组质粒pET32a(+)-zbtb38,再转入大肠杆菌(Escherichia coli)感受态细胞BL21(DE3)中诱导表达,通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)、免疫印迹(Western blot)和液相色谱-质谱联用(LC-MS)等技术进行鉴定。用纯化后的重组蛋白制备兔抗斑点叉尾鮰ZBTB38多克隆抗体,通过酶联免疫吸附(ELISA)和Western blot技术检测抗体效价及其特异性,最后采用Western blot方法检测ZBTB38在性腺中的表达情况,并使用实时荧光定量PCR(qRT-PCR)技术对检测结果进行验证。结果表明,制备的兔抗斑点叉尾鮰ZBTB38多克隆抗体效价可达1:(5.12×10^(5)),能够特异性识别斑点叉尾鮰性腺组织中表达的ZBTB38蛋白,其中精巢组织中的表达水平显著高于卵巢,Western blot与qRT-PCR检测结果较为一致。综上所述,研究成功制备了斑点叉尾鮰ZBTB38的多克隆抗体,为下一步深入研究斑点叉尾鮰zbtb38基因的功能奠定了基础。

再植指功能恢复效果的列线图预测模型构建

目的探讨再植指功能恢复差的危险因素,建立患指功能恢复差的预测模型。方法回顾性分析2017年5月至2022年5月在唐山市人民医院行断指再植术的309例(断指376个)手指离断患者的资料,其中男性231例断指274个,女性78例断指102个;年龄19~67岁,平均(41.57±8.59)岁。术后1年对患指功能的恢复效果进行判定,并分成恢复差组和对照组。收集患者一般人口特征、病情信息、手术信息、术前血常规指标行单变量筛查,通过多因素分析确定患指功能恢复差的危险因素,以森林图展现各指标效应大小。依据预测变量构建列线图模型,并通过受试者工作特征(receiver operating characteristic,ROC)曲线下面积(area under curve,AUC)、校准曲线及决策曲线(decision curve analysis,DCA)检验其效能。结果309例(断指376个)患者中,有68例(断指81个)术后功能恢复差,占比21.54%。两组吸烟史、损伤性质、离断程度、离断平面、断指暴露时间、断指保存方式、是否遵医开展康复锻炼情况比较差异有统计学意义(P<0.05)。Logistic回归分析显示,术后患指功能恢复差的危险因素主要为有吸烟史、撕脱伤、离断平面为非末节指骨、断指暴露时间≥8 h、未遵医开展康复锻炼。内部验证显示,AUC为0.819(95%CI:0.762~0.875),模型预测患指功能恢复差的概率与实际概率高度吻合,拟合优度HL检验χ^(2)=7.389,P=0.286。DCA显示模型在风险阈值12%~75%时具有较好的实用性。结论根据有吸烟史、损伤性质、离断平面、断指暴露时间、是否遵医开展康复锻炼构建的列线图模型对断指再植术后患指功能恢复差的预测效果较好。