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血红素氧合酶-1高表达抗兔心肌缺血再灌注损伤研究 被引量:1
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作者 黄维义 张英 +2 位作者 严丽 彭永权 陈蓉 《中国现代医学杂志》 CAS CSCD 北大核心 2006年第10期1477-1479,1482,共4页
目的探讨血红素氧合酶-1(HO-1)高表达抗兔心肌IR损伤的效果及其作用机制。方法32只兔随机均分为单纯IR组与HO-1诱导组,分别预先给予生理盐水5mL及血晶素25mg/kg腹腔内注入,24h后,开胸结扎冠脉左前降支缺血40min,松开再灌注4h,测定心肌中... 目的探讨血红素氧合酶-1(HO-1)高表达抗兔心肌IR损伤的效果及其作用机制。方法32只兔随机均分为单纯IR组与HO-1诱导组,分别预先给予生理盐水5mL及血晶素25mg/kg腹腔内注入,24h后,开胸结扎冠脉左前降支缺血40min,松开再灌注4h,测定心肌中HO-1表达及活性、中性粒细胞浸润、丙二醛(MDA)含量、心肌梗死范围及心肌细胞凋亡指数。结果与单纯IR组比较,HO-1预诱导组心肌中HO-1表达及活性均明显升高(P<0.01),中性粒细胞浸润及MDA含量明显降低(P<0.01),心肌梗死范围及心肌细胞凋亡指数均显著减少(P<0.01)。结论HO-1可通过抗炎、抗氧化等作用减轻急性心肌IR损伤。 展开更多
关键词 血红素氧合酶-1 再灌注损伤 凋亡 中性粒细胞
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利用椭圆小球藻硝酸还原酶缺失突变体为生物反应器表达兔防御素NP-1蛋白 被引量:4
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作者 张小宇 王鹏 +5 位作者 赵世民 李霞 沈昕 孙勇如 储成才 王义琴 《遗传》 CAS CSCD 北大核心 2006年第12期1580-1584,共5页
利用转基因小球藻为生物反应器生产兔防御素NP-1蛋白具有重要的应用价值。本研究利用椭圆小球藻(Chlorellaellipsoidea)硝酸还原酶(nitratereductase)缺失突变体为受体,构建了包含NPTII基因和硝酸还原酶基因两个筛选标记的兔防御素蛋白... 利用转基因小球藻为生物反应器生产兔防御素NP-1蛋白具有重要的应用价值。本研究利用椭圆小球藻(Chlorellaellipsoidea)硝酸还原酶(nitratereductase)缺失突变体为受体,构建了包含NPTII基因和硝酸还原酶基因两个筛选标记的兔防御素蛋白表达载体,采用电激法将目的基因转入椭圆小球藻硝酸还原酶缺失突变体nrm-4,获得了正确表达防御素蛋白的转基因藻,从而表明通过硝酸还原酶作为筛选标记基因并结合硝酸还原酶缺失突变体可作为较好的小球藻生物反应器生产模式。 展开更多
关键词 椭圆小球藻 硝酸还原酶缺失突变体 兔防御素
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外伤性兔眼内炎房水中肿瘤坏死因子-α、白细胞介素-1β及中性粒细胞趋化因子-1的相对含量变化及意义 被引量:9
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作者 黄云云 罗予 +1 位作者 张金嵩 魏丽娟 《眼科新进展》 CAS 北大核心 2013年第8期713-716,共4页
目的通过分析兔眼外伤后不同时间点房水中肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白细胞介素-1β(interleukin-1β,IL-1β)、中性粒细胞趋化因子-1(cytokine induced neutrophil chemoattractant-1,CINC-1)含量的动态变化... 目的通过分析兔眼外伤后不同时间点房水中肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白细胞介素-1β(interleukin-1β,IL-1β)、中性粒细胞趋化因子-1(cytokine induced neutrophil chemoattractant-1,CINC-1)含量的动态变化,探讨促炎细胞因子在外伤性眼内炎中的作用机制。方法将18只兔随机分为3组,每组6只,其中Ⅰ组为正常对照组,Ⅱ组为无菌外伤组,Ⅲ组为有菌(金黄色葡萄球菌)外伤组。Ⅱ组和Ⅲ组兔右眼建立外伤性眼内炎模型,并将金黄色葡萄球菌接种于Ⅲ组兔右眼的玻璃体内。于造模后6h、12h、1d、2d、4d、5d、7d、14d用裂隙灯及间接检眼镜观察眼部炎症情况并进行临床炎症评分。ELISA法测定各组造模后6h、12h、1d、2d、4d、5d房水中TNF-α、IL-1β、CINC-1的含量变化。结果造模后12h、1d、2d、4d、5d、7d、14d,Ⅱ组各时间点临床炎症评分分别为(2.50±0.55)分、(3.67±0.82)分、(4.00±0.63)分、(6.12±0.75)分、(7.00±0.63)分、(7.33±0.52)分、(5.50±0.54)分,Ⅲ组分别为(4.00±0.63)分、(6.17±0.75)分、(7.67±0.52)分、(10.60±0.82)分、(11.50±0.55)分、(11.80±0.41)分、(10.33±0.51)分,两组各时间点临床炎症评分间差异均有统计学意义(均为P<0.05),造模12h后Ⅲ组炎症反应明显较Ⅱ组重。Ⅱ组和Ⅲ组兔房水中TNF-α在炎症初期就开始升高,1d时达到高峰,与Ⅰ组相比差异均有统计学意义(均为P<0.05)。Ⅱ组和Ⅲ组兔房水中IL-1β含量于造模后1d时达到高峰,与Ⅰ组相比差异均有统计学意义(均为P<0.05)。Ⅱ组和Ⅲ组兔房水中CINC-1含量在造模后12h时即达到高峰,与Ⅰ组相比差异均有统计学意义(均为P<0.05)。Ⅱ组和Ⅲ组兔房水中TNF-α、IL-1β、CINC-1各时间点相比差异均无统计学意义(均为P>0.05)。结论外伤后机体的免疫调节机制在外伤性眼内炎发生发展过程中发挥重要作用,房水中TNF-α、IL-1β、CINC-1表达升高与炎症程度相关,调控细胞因子网络可能成为未来眼内炎一个有效的联合治疗措施。 展开更多
关键词 外伤性眼内炎 肿瘤坏死因子-Α 白细胞介素-1Β 中性粒细胞趋化因子-1 金黄色葡萄球菌
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Protective effects of α_1 -antitrypsin on acute lung injury in rabbits induced by endotoxin 被引量:11
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作者 揭志军 蔡映云 +3 位作者 杨文兰 金美玲 朱威 祝慈芳 《Chinese Medical Journal》 SCIE CAS CSCD 2003年第11期1678-1682,共5页
Objective To investigate whether pretreatment with α1,-antitrypsin (AAT) can attenuate acute lung injury (ALI) in rabbits induced with endotoxin.Methods Thirty-two healthy adult New Zealand rabbits were anaesthetized... Objective To investigate whether pretreatment with α1,-antitrypsin (AAT) can attenuate acute lung injury (ALI) in rabbits induced with endotoxin.Methods Thirty-two healthy adult New Zealand rabbits were anaesthetized, tracheotomized and mechanically ventilated. They were then randomly divided into four groups (n =8): (1) Infusion of Escherichia coli endotoxin [ Lipopolysaccharide (LPS) 500μg/kg ] without AAT (Group LPS). (2) Infusion of AAT 120 mg/kg at 15 minutes after LPS (Group LAV). (3) Infusion of AAT 120 mg/kg without endotoxin (Group AAT). (4) Infusion of saline 4 ml/kg as control (Group NS). Arterial blood gases, peripheral leukocyte counts and airway pressure were recorded every hour for eight hours. Physiologic intrapulmonary shunting (Qs/Qt) was measured every four hours. After eight hours, blood samples were collected for measurement of plasma concentration and activity of AAT. Then, the animals were sacrificed, and bronchoalveolar lavage fluid (BALF) was collected for measurement of concentrations of total protein (TP), interleukin-8 (IL-8), tumor necrosis factor (TNFa, the activities of NE and AAT, total phospholipids (TPL) and disaturated phosphatidylcholine (DSPC). In addition, the wet-to-dry lung weight ratio (W/D) was measured.Results The infusion of endotoxin induced decreases in arterial oxygen pressure (PaO2), peripheral leukocyte counts, total respiratory compliance (TLC) and the increases in peak pressure (Ppeak), Qs/ Qt compared with the baseline values ( P < 0. 05). The increased plasma concentration but reduced activity of AAT was also found in contrast to that in Group NS (P<0. 05). In the BALF, the activity of AAT, TPL, DSPC/TPL were lower than those in Group NS (P<0. 05), but the concentrations of albumin, IL-8, TNFα, the activity of NE and the ratio of W/D were higher than those in Group NS (P <0. 05). The pretreatment of AAT attenuated the deterioration of oxygenation, the reduction of compliance and the deterioration of other physiological and biochemical parameters mentioned above.Conclusion Pretreatment with AAT could attenuate endotoxin-induced lung injury in rabbits. Those beneficial effects of AAT might be due, in part, to reduction in the levels of mediators that could activate neutrophils, in addition to the direct inhibitory effect on neutrophil elastase. 展开更多
关键词 acute lung injury ·α1-antitrypsin neutrophil elastase ·rabbits ENDOTOXIN
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